pLV3-CMV-CCL2-3×HA-EF1a-mCherr

pLV3-CMV-CCL2-3×HA-EF1a-mCherry-Puro plasmid

PVT95127

pLV3-CMV-CCL2-3×HA-EF1a-mCherry-Puro plasmid Information

Plasmid name: pLV3-CMV-CCL2(human)-3×HA-EF1a-mCherry-Puro
Alias: NM_002990.5 ;A-152E5.1; ABCD-1; DC/B-CK; MDC; SCYA22;STCP-1
Gene lenght: 279bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Red
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-CCL2-3×HA-EF1a-mCherry-Puro plasmid Description

pLV3-CMV-CCL2-3×HA-EF1a-mCherry-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-CCL2-3×HA-EF1a-mCherry-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatggatcg cctacagact gcactcctgg ttgtcctcgt 2341 cctccttgct gtggcgcttc aagcaactga ggcaggcccc tacggcgcca acatggaaga 2401 cagcgtctgc tgccgtgatt acgtccgtta ccgtctgccc ctgcgcgtgg tgaaacactt 2461 ctactggacc tcagactcct gcccgaggcc tggcgtggtg ttgctaacct tcagggataa 2521 ggagatctgt gccgatccca gagtgccctg ggtgaagatg attctcaata agctgagcca 2581 aggatccTAC CCATACGACG TACCTGATTA TGCAACTTAT CCTTATGATG TACCTGATTA 2641 TGCTAGCTAT CCTTATGATG TCCCAGACTA CGCATAGgcg gccgcGaagg atctgcgatc 2701 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg 2761 gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg 2821 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag 2881 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc 2941 gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc 3001 ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct 3061 aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac 3121 ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg 3181 tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgctagacg 3241 ccaccatggt gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct 3301 tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg 3361 agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc 3421 tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga 3481 agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg 3541 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc 3601 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc 3661 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg 3721 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg 3781 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca 3841 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt 3901 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagggatctc 3961 gcgagggcag aggaagtctt ctaacatgcg gtgacgtgga ggagaatccc ggccctatga 4021 ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca 4081 ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc 4141 acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg 4201 gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg 4261 tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc 4321 ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg 4381 cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg 4441 ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga 4501 cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg 4561 tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaaatc 4621 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 4681 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 4741 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 4801 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 4861 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 4921 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 4981 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct 5041 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 5101 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc 5161 ttcgccctca gacgagtcgg atctcccttt ggccgcctcc ccgcctggta cctttaagac 5221 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg 5281 aagggctaat tcactcccaa cgaaaataag atctgctttt tgcttgtact gggtctctct 5341 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc 5401 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg 5461 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt 5521 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga 5581 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca 5641 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat 5701 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc 5761 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga 5821 gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca gagacggccc 5881 aaattcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc 5941 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta 6001 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca 6061 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc 6121 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc 6181 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat 6241 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt 6301 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg 6361 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc 6421 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt 6481 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa 6541 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta 6601 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa 6661 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa 6721 ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt 6781 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt 6841 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat 6901 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat 6961 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc 7021 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc 7081 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta 7141 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga 7201 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg 7261 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc 7321 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat 7381 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag 7441 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat 7501 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa 7561 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa 7621 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga 7681 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg 7741 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc 7801 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg 7861 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact 7921 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat 7981 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt 8041 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 8101 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 8161 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 8221 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca 8281 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa 8341 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt 8401 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag 8461 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct 8521 g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.