pAAV-EF1a-Kctd20-EGFP plasmid

pAAV-EF1a-Kctd20-EGFP plasmid

PVT95125

pAAV-EF1a-Kctd20-EGFP plasmid Information

Plasmid name: pAAV-EF1a-Kctd20(mouse)-EGFP
Alias: NM_025888.7;2410004N11Rik; D17Ertd562e
Gene lenght: 1257bp
Host: Mammalian cells,Adeno associated virus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EF1a-R-F
Reverse primer:
Copy number: High
Induction:

pAAV-EF1a-Kctd20-EGFP plasmid Description

pAAV-EF1a-Kctd20-EGFP is a plasmid suitable for use in mammalian cells, and can also be used for adeno-associated virus packaging.

pAAV-EF1a-Kctd20-EGFP plasmid Sequence

1 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg 61 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga 121 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt 181 ttgctcacat gtcctgcagg cagctgcgcg ctcgctcgct cactgaggcc gcccgggcgt 241 cgggcgacct ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc 301 aactccatca ctaggggttc ctgcggccgc acgcgtGAGG GCCTATTTCC CATGATTCCT 361 TCATATTTGC ATATACGATA CAAGGCTGTT AGAGAGATAA TTGGAATTAA TTTGACTGTA 421 AACACAAAGA TATTAGTACA AAATACGTGA CGTAGAAAGT AATAATTTCT TGGGTAGTTT 481 GCAGTTTTAA AATTATGTTT TAAAATGGAC TATCATATGC TTACCGTAAC TTGAAAGTAT 541 TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAA ACTCTAGACC GCAGGTATGC 601 ACGCGTGAAT TCacgcgtaa gctttgcaaa gatggataaa gttttaaaca gagaggaatc 661 tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc ggtgcccgtc 721 agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt 781 gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc gtgtactggc 841 tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc gccgtgaacg 901 ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg 961 ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc acctggctgc 1021 agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt 1081 gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg cgctggggcc 1141 gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat aagtctctag 1201 ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat agtcttgtaa 1261 atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc ggcgacgggg 1321 cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa 1381 tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc gcgccgccgt 1441 gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg tgagcggaaa 1501 gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag 1561 agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca gccgtcgctt 1621 catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc tcgagctttt 1681 ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt ccccacactg 1741 agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc ttggaatttg 1801 ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt caaagttttt 1861 ttcttccatt tcaggtgtcg tgaggtaccg gatccgccac catgaatgtt caccagggca 1921 gtgatggcga ctggtcattg cagccagagc tcagctgcct aggagatgag gccttagctg 1981 ccactcaaga gaaagaaggc agcagcttgg tgtcctctgg tctgcatagt gtcacttatc 2041 ccctagctgc caggagtgaa gaccttgcac ttgactatgc ctctcagcca gcaagccttc 2101 cgcatcctca cataatgcca cttcctgaag acaacaaggg ctcatgcttc cagagtggga 2161 gcaaacggag ccacgagccc ttcattgtgc cagagagatt tggaaacagc ggcttaggtt 2221 ttggaggtgg cgctcactct caagccccag agaaggtgac acttcttgtg gatggcactc 2281 gttttgtggt aaatcctcag attttcactg ctcatccgga taccatgttg ggaaggatgt 2341 ttgggccggg aagagaatac aacttcacaa ggcccaatga aaagggggag tatgtgatcg 2401 cggaggggat cagcgccacc gtgttccgca cagtcctgga ttattacaaa actggcatca 2461 tcaactgtcc agatggcatc tctatcccag acctcagaga cacgtgtgac tatctctgca 2521 ttaacttcga cttcaacact atccgatgtc aggatctgag tgctctactg cacgagctct 2581 ccaacgatgg tgcccacaag cagtttgacc actacctcga ggaactgatt ctgcccatca 2641 tggtgggctg tgccaagaaa ggcgagcgag agtgtcacat tgtcgtgctg acagacgagg 2701 actctgtgga ctgggatgaa gaccaccccc cacccatggg ggaagagtat tcccaaattc 2761 tttacagctc caagctctac agattcttca aatatatcga gaatagagat gttgctaaaa 2821 ctgtgttaaa ggaacgaggc ctgaagaaca tccgcattgg aattgaaggt taccctacct 2881 gtaaagagaa gatcaagagg agacctggcg ggcggtctga agtcatctac aattatgtgc 2941 agcgcccttt tatccagatg tcatgggaaa aagaggaagg aaagagtcgc catgtggatt 3001 tccagtgcgt tcgcagcaaa tccctcacta atttggtggc tgctggagag gatgtcttag 3061 aagaccagga gataataatg catcacccac cacaagtaga tgaacttgac cgcttaaatg 3121 ctccactctc tcagatggct ccgaatgact ttcaagatgg tggaggcgga tcaggaggcg 3181 gtggatctat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg 3241 agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg 3301 ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct 3361 ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc 3421 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca 3481 ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg 3541 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc 3601 tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc 3661 agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc 3721 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg 3781 acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc 3841 acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt 3901 acaagtaaag cggccgcact cgagatatca agcttatcga taatcaacct ctggattaca 3961 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat 4021 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct 4081 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac 4141 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca 4201 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca 4261 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg 4321 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctatgtt gccacctgga 4381 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt 4441 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga 4501 gtcggatctc cctttgggcc gcctccccgc atcgataccg agcgctgctc gagagatcta 4561 cgggtggcat ccctgtgacc cctccccagt gcctctcctg gccctggaag ttgccactcc 4621 agtgcccacc agccttgtcc taataaaatt aagttgcatc attttgtctg actaggtgtc 4681 cttctataat attatggggt ggaggggggt ggtatggagc aaggggcaag ttgggaagac 4741 aacctgtagg gcctgcgggg tctattggga accaagctgg agtgcagtgg cacaatcttg 4801 gctcactgca atctccgcct cctgggttca agcgattctc ctgcctcagc ctcccgagtt 4861 gttgggattc caggcatgca tgaccaggct cagctaattt ttgttttttt ggtagagacg 4921 gggtttcacc atattggcca ggctggtctc caactcctaa tctcaggtga tctacccacc 4981 ttggcctccc aaattgctgg gattacaggc gtgaaccact gctcccttcc ctgtccttct 5041 gattttgtag gtaaccacgt gcggaccgag cggccgcagg aacccctagt gatggagttg 5101 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga 5161 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg 5221 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa 5281 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc 5341 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt 5401 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag 5461 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt 5521 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt 5581 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt 5641 cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt 5701 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc 5761 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg 5821 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg 5881 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa 5941 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga 6001 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 6061 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 6121 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg 6181 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag 6241 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg 6301 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg 6361 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt 6421 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga 6481 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac 6541 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc 6601 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc 6661 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac 6721 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag 6781 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg 6841 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta 6901 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg 6961 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata 7021 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 7081 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 7141 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 7201 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 7261 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag 7321 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 7381 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 7441 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 7501 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 7561 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 7621 tcg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.