pCMV-USP24-3×HA-Neo plasmid

pCMV-USP24-3×HA-Neo plasmid

PVT95108

pCMV-USP24-3×HA-Neo plasmid Information

Plasmid name: pCMV-USP24(human)-3×HA-Neo
Alias: NM_015306.3
Gene lenght: 7860bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pCMV-USP24-3×HA-Neo plasmid Description

pCMV-USP24-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-USP24-3×HA-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatgga atcggaggag 721 gagcagcaca tgaccacgct gctgtgcatg ggcttctcag accccgccac catccgcaag 781 gccctgcgcc tggccaagaa cgacattaac gaggccgtgg cactgctcac caacgagcgg 841 ccgggcctcg actacggcgg ctacgagccc atggacagcg gcggtggccc cagccccggg 901 cccggcgggg gcccgcgggg cgacggcgga ggtgacggcg gcggcggcgg cccctcccgc 961 ggcgggagca ccggaggcgg gggcggcttc gaccccccgc ccgcctacca cgaggtggtg 1021 gacgcggaga agaatgatga gaatggaaac tgctcagggg aaggaattga attccctaca 1081 acaaatttat atgaactgga aagccgtgtt ttgactgatc attggtccat cccttacaag 1141 cgagaagaat cactaggcaa atgcctgttg gcatctacct acctagcaag acttggtctt 1201 tccgagtctg atgagaattg tagaaggttt atggacaggt gtatgcctga agcatttaaa 1261 aagctcctga catcaagtgc tgttcacaag tggggtactg aaattcatga aggaatttac 1321 aacatgttga tgctattaat agaactggtc gcagagagaa taaaacaaga tccaattccc 1381 actggtctcc tgggtgtgct tacaatggct ttcaatcctg ataatgaata ccattttaaa 1441 aacagaatga aagtgtctca aaggaattgg gcagaagtgt ttggagaggg aaatatgttt 1501 gctgtttcac ctgtatcgac tttccaaaag gagcctcatg gatgggttgt ggatttggta 1561 aataagtttg gagaattagg tggatttgca gcaatccaag ccaagctcca ttcagaagat 1621 atagaacttg gggctgtctc agcactgatt cagcccttag gagtgtgtgc agagtacctc 1681 aattcctccg tggtacagcc catgctagac ccagtcattc ttactacaat ccaggatgta 1741 cggagtgtag aagagaaaga cctcaaagac aagagattgg ttagcatccc tgagctcttg 1801 tctgccgtta agttactttg catgcgcttc caaccggatc tggtgacaat tgtggatgac 1861 cttcgactag atattctatt gcgcatgctg aaatcaccac atttcagtgc taagatgaat 1921 tctctcaaag aagtaaccaa actaatagaa gatagcactt tatccaaatc tgtgaagaat 1981 gctatagata cagacagatt attagattgg ctagttgaaa actcagttct gtcgattgca 2041 ctggaaggca acatagacca agcacaatac tgtgaccgta taaagggaat tattgaactc 2101 ttgggtagta aattgtcgtt agatgaactc actaaaattt ggaagataca gtcaggacaa 2161 tcatctactg tgattgagaa cattcatact attattgctg cagcggctgt gaaatttaat 2221 tcagatcagc ttaatcattt gtttgttctc attcagaaga gctgggagac tgagagtgat 2281 agagtaagac agaagctttt gagcctgatt ggacgaatag gccgggaagc tcgctttgag 2341 accacttctg gaaaggtttt agacgtactc tgggaactgg ctcaccttcc aaccctgccc 2401 agtagcctta ttcagcaggc cttggaggag cacctgacaa tccttagtga tgcatatgca 2461 gtgaaagaag caatcaagag gagctacatc atcaagtgca tagaagatat taagaggcct 2521 ggagaatggt caggtttgga aaaaaacaag aaggatggat tcaagtcatc tcagcttaat 2581 aatccccagt ttgtatgggt ggtaccagct ttgcgtcagc tccatgaaat tactcgctca 2641 ttcataaaac aaacctatca aaagcaagac aagagcatta ttcaagactt gaagaagaat 2701 tttgaaatag tgaaattggt aacgggaagt ttgatcgctt gtcatcggct tgcagctgct 2761 gtggccgggc ctggaggctt aagtggctcg acactagtgg atggccggta cacttaccgg 2821 gagtatttag aggcacatct aaaatttcta gcgtttttct tgcaagaagc tactctgtat 2881 ctgggctgga atcgtgccaa ggagatctgg gagtgtcttg taactggcca ggatgtttgt 2941 gaattagata gagagatgtg ttttgaatgg tttacaaaag gacagcatga tcttgagagt 3001 gatgttcagc agcagctctt caaggagaaa attcttaaat tggagtcata tgaaatcact 3061 atgaatggtt ttaacttatt taaaactttt tttgaaaatg tgaatctttg tgatcatcga 3121 ttgaaaagac aaggagctca gttgtatgta gaaaagctgg aattgatagg aatggatttc 3181 atttggaaaa tagccatgga atcacctgat gaagaaattg ctaatgaagc tattcagcta 3241 atcataaact atagttacat taatctaaat cctagattaa agaaggattc agtatcttta 3301 cataagaaat tcattgctga ttgctacaca agattagaag cagccagttc agcacttggt 3361 ggccccactc taacacatgc tgtgaccaga gcaacaaaaa tgcttacagc aactgccatg 3421 ccaactgtag caacctcagt tcagtctcct tatagatcta ctaaacttgt aataattgag 3481 agattgctgc ttctggcaga gcgctatgtg atcactatag aggattttta ctctgttcca 3541 cgaactattc tacctcatgg tgcctcattt catggacatc ttttaaccct taatgttacc 3601 tatgagtcta ccaaagatac cttcactgtc gaggctcaca gtaatgaaac catagggagt 3661 gtccggtgga aaatagccaa gcagttgtgc tctcctgtgg ataatataca gatatttaca 3721 aatgatagcc tgctgacagt gaataaagat caaaagctac tccaccaact gggcttttct 3781 gatgaacaaa tccttacagt gaagacttct ggcagtggga ccccatctgg gagttcagca 3841 gattcttcaa ccagctccag cagcagcagc agtggggttt ttagttcttc atatgccatg 3901 gagcaggaga aatccctccc tggtgtagtg atggctctcg tatgtaacgt atttgacatg 3961 ctttatcagc tcgccaatct ggaagagcca aggataactc tacgagtacg gaagcttctg 4021 ctcttgatac ccactgatcc agccattcag gaagcccttg atcaacttga ttctttagga 4081 agaaagaaaa cattgctgtc tgaatcaagt tctcagtcct caaaatctcc atccctgtca 4141 tcaaagcaac agcaccagcc aagtgccagt tcaattttag aaagtctgtt tcgatctttt 4201 gccccgggaa tgtctacctt cagagtgctc tacaacttag aagttctaag ctccaaactc 4261 atgccaacag ctgatgatga catggccaga agctgtgcca aatccttctg tgaaaacttc 4321 ctcaaagctg gcggtttgag tttggttgta aatgtcatgc agagagactc catcccatca 4381 gaagtagact atgaaacaag gcagggtgtt tattccatct gtctacagct tgcaagattt 4441 ttacttgtcg gacaaacaat gcccacgtta ttagatgaag acctcaccaa agatggtata 4501 gaagcacttt cttcccgccc attccgaaat gtcagccggc agacaagcag acagatgtcc 4561 ttatgtggta ccccagaaaa gtcatcctac cgacagttgt ccgtgtctga taggtcttct 4621 attagggttg aggaaatcat ccctgctgct cgagttgcaa tacaaacaat ggaagtaagt 4681 gatttcactt ctactgtggc ttgcttcatg agattgtcat gggctgcggc tgcaggacgg 4741 cttgatcttg ttgggagtag ccagccaatt aaagaaagta attccctgtg tcctgctgga 4801 attcgaaaca gactcagcag ttcaggaagc aattgcagct ctggaagtga aggagaacca 4861 gtagccctgc atgcgggaat ctgtgttcga caacagtctg tatccaccaa agactcgctg 4921 attgcgggag aggctttgtc tcttcttgtt acgtgcctac agcttcggag ccagcaactg 4981 gcatctttct ataacttgcc ctgtgttgct gatttcatca ttgatattct gctcggatca 5041 ccaagtgctg agattcgccg ggttgcctgt gatcagctgt acactcttag tcagacagac 5101 acatcagcgc atccagatgt gcagaagcca aatcagtttc ttctaggcgt aatcctcacg 5161 gctcagctgc ctctctggtc tccaactagt attatgagag gagtcaatca gagactgtta 5221 tctcagtgta tggagtattt tgatttgaga tgccagttat tagatgatct gacaacttca 5281 gaaatggagc agttaaggat cagcccagct acgatgcttg aagatgagat tacttggctg 5341 gataactttg aacctaatcg tacagctgaa tgtgagacca gtgaagcgga caacatctta 5401 ctggcagggc acttacgcct catcaagacc cttctttcac tctgtggggc agaaaaggaa 5461 atgcttggtt catcactcat taaaccattg ttagatgact tccttttccg agcttctaga 5521 attattttaa atagtcattc tccagctggc agtgccgcca tcagtcaaca ggactttcat 5581 ccaaagtgta gtacagcgaa tagccgattg gcagcctatg aagtccttgt gatgttggct 5641 gatagttcac cttcaaatct tcaaattatt ataaaagaac tgctttctat gcatcaccag 5701 cctgaccctg ctcttaccaa ggagtttgat taccttcccc cagtggatag caggtccagt 5761 tcagggtttg tggggctgag aaatggtggt gcaacttgtt atatgaatgc agtcttccag 5821 cagctgtata tgcaacctgg gctccctgag tcattacttt cagtggatga tgacacagac 5881 aatccagatg atagcgtgtt ttaccaagtg cagtctctct ttggacattt aatggaaagc 5941 aagctgcagt actatgtacc tgagaatttt tggaagattt tcaagatgtg gaataaagaa 6001 ctttatgtga gagaacagca ggatgcatat gaattcttta ctagtctcat tgatcagatg 6061 gatgaatacc tcaagaaaat ggggagagac caaattttta agaatacatt tcagggcatc 6121 tactctgatc agaagatctg taaagactgt cctcacagat atgagcgtga agaagctttc 6181 atggctctca atctaggagt gacttcttgt cagagtttgg aaatttcttt ggaccaattt 6241 gttagaggag aagttctaga aggaagtaat gcgtactact gtgaaaagtg taaagaaaag 6301 agaataacag tgaaaaggac ctgtattaaa tctttaccta gcgtcttggt aattcaccta 6361 atgagatttg ggtttgactg ggaaagcgga cgctccatta aatatgatga acaaataagg 6421 tttccctgga tgctaaacat ggagccttac acagtttcag gaatggctcg ccaagattct 6481 tcttctgaag ttggggaaaa tgggcgaagt gtggatcagg gcggtggagg atccccacga 6541 aaaaaggttg ccctcacaga aaactatgaa cttgtcggtg tcatcgtaca cagtgggcag 6601 gcacacgcag gccactacta ttccttcatt aaggacaggc gagggtgtgg aaaaggaaag 6661 tggtataaat ttaatgacac agttatagaa gaatttgacc taaatgacga gaccctggag 6721 tatgaatgct ttggaggaga atatagacca aaagtttatg atcaaacaaa cccatacact 6781 gatgtgcgcc gaagatactg gaatgcctat atgcttttct accaaagggt gtctgatcag 6841 aactccccag tattaccaaa gaaaagtcga gtcagcgttg tacggcagga agctgaggat 6901 ctctctctgt cagctccatc ttcaccagaa atttcacctc agtcatcccc tcggccccat 6961 aggccgaaca atgaccggct gtctattctt accaagctgg ttaaaaaagg cgagaagaaa 7021 ggactgtttg tggagaaaat gcctgctcga atataccaga tggtgagaga tgagaacctc 7081 aagtttatga agaatagaga tgtatacagt agtgattatt tcagttttgt tttgtcttta 7141 gcttcattga atgctactaa attaaagcat ccatattatc cttgcatggc aaaggtgagc 7201 ttacagcttg ctattcaatt cctttttcaa acttatctac ggacaaagaa gaaactcagg 7261 gttgatactg aagaatggat tgctaccatt gaagcattgc tttcaaaaag ttttgatgct 7321 tgtcagtggt tagttgaata ttttattagt tctgaaggac gagaattgat aaagattttc 7381 ttactggagt gcaatgtgag agaagtacga gttgctgtgg ccaccattct ggagaaaacc 7441 ctagacagtg ccttgtttta tcaggataag ttaaaaagcc ttcatcagtt actggaggta 7501 ctacttgctc tgttggacaa agacgtccca gaaaattgta aaaactgtgc tcagtacttt 7561 ttcctgttca acacttttgt acaaaagcaa ggaattaggg ctggagatct tcttctgagg 7621 cattcagctc tgcggcacat gatcagcttc ctcctagggg ccagtcggca aaacaatcag 7681 atacgtcgat ggagttcagc acaagcacga gaatttggga atcttcacaa tacagtggcg 7741 ttacttgttt tgcattcaga tgtctcatcc caaaggaatg ttgctcctgg catatttaag 7801 caacgaccac ccattagcat tgctccctca agccctctgt tgcccctcca tgaggaggta 7861 gaagccttgt tgttcatgtc tgaagggaaa ccttacctgt tagaggtaat gtttgctttg 7921 cgggagctga caggctcgct cttggcactc attgagatgg tagtgtactg ctgtttctgt 7981 aatgagcatt tttccttcac aatgctgcat ttcattaaga accaactaga aacggctcca 8041 cctcatgagt taaagaatac gttccaacta cttcatgaaa tattggttat tgaagatcct 8101 atacaagtag agcgagtcaa atttgtgttt gagacagaaa atggattact agctttgatg 8161 caccacagta atcatgtgga cagtagtcgc tgctaccagt gtgtcaaatt tcttgtcact 8221 cttgctcaaa agtgtcctgc agctaaggag tacttcaagg agaattccca ccactggagc 8281 tgggctgtgc agtggctaca gaagaagatg tcagaacatt actggacacc acagagtaat 8341 gtctctaatg aaacatcaac tggaaaaacc tttcagcgaa ccatttcagc tcaggacacg 8401 ttagcgtatg ccacagcttt gttgaatgaa aaagagcaat caggaagcag taatgggtcg 8461 gagagtagtc ctgccaatga gaacggagac aggcatctac agcagggttc agaatctccc 8521 atgatgattg gtgagttgag aagtgacctt gatgatgttg atcccCTCGA GTACCCATAC 8581 GACGTACCTG ATTATGCAAC TTATCCTTAT GATGTACCTG ATTATGCTAG CTATCCTTAT 8641 GATGTCCCAG ACTACGCATA GTGAGGGCCC GATATCTCGA CAATCAACCT CTGGATTACA 8701 AAATTTGTGA AAGATTGACT GGTATTCTTA ACTATGTTGC TCCTTTTACG CTATGTGGAT 8761 ACGCTGCTTT AATGCCTTTG TATCATGCTA TTGCTTCCCG TATGGCTTTC ATTTTCTCCT 8821 CCTTGTATAA ATCCTGGTTG CTGTCTCTTT ATGAGGAGTT GTGGCCCGTT GTCAGGCAAC 8881 GTGGCGTGGT GTGCACTGTG TTTGCTGACG CAACCCCCAC TGGTTGGGGC ATTGCCACCA 8941 CCTGTCAGCT CCTTTCCGGG ACTTTCGCTT TCCCCCTCCC TATTGCCACG GCGGAACTCA 9001 TCGCCGCCTG CCTTGCCCGC TGCTGGACAG GGGCTCGGCT GTTGGGCACT GACAATTCCG 9061 TGGTGTTGTC GGGGAAGCTG ACGTCCTTTC CATGGCTGCT CGCCTGTGTT GCCACCTGGA 9121 TTCTGCGCGG GACGTCCTTC TGCTACGTCC CTTCGGCCCT CAATCCAGCG GACCTTCCTT 9181 CCCGCGGCCT GCTGCCGGCT CTGCGGCCTC TTCCGCGTCT TCGCCTTCGC CCTCAGACGA 9241 GTCGGATCTC CCTTTGGGCC GCCTCCCCGC CTGGAAACGG GGGAGGCTAA CTGAAACACG 9301 GAAGGAGACA ATACCGGAAG GAACCCGCGC TATGACGGCA ATAAAAAGAC AGAATAAAAC 9361 GCACGGGTGT TGGGTCGTTT GTTCATAAAC GCGGGGTTCG GTCCCAGGGC TGGCACTCTG 9421 TCGATACCCC ACCGAGACCC CATTGGGGCC AATACGCCCG CGTTTCTTCC TTTTCCCCAC 9481 CCCACCCCCC AAGTTCGGGT GAAGGCCCAG GGCTCGCAGC CAACGTCGGG GCGGCAGGCC 9541 CTGCCATAGC AGATCTGCGC AGCTGGGGCT CTAGGGGGTA TCCCCACGCG CCCTGTAGCG 9601 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG 9661 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC 9721 CCCGTCAAGC TCTAAATCGG GGCATCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC 9781 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA 9841 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA 9901 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGGGGA 9961 TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTAATTCT 10021 GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG GCAGAAGTAT 10081 GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG GCTCCCCAGC 10141 AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC CGCCCCTAAC 10201 TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC ATGGCTGACT 10261 AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCTGCC TCTGAGCTAT TCCAGAAGTA 10321 GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCCGGGAG CTTGTATATC 10381 CATTTTCGGA TCTGATCAAG AGACAGGATG AGGATCGTTT CGCATGATTG AACAAGATGG 10441 ATTGCACGCA GGTTCTCCGG CCGCTTGGGT GGAGAGGCTA TTCGGCTATG ACTGGGCACA 10501 ACAGACAATC GGCTGCTCTG ATGCCGCCGT GTTCCGGCTG TCAGCGCAGG GGCGCCCGGT 10561 TCTTTTTGTC AAGACCGACC TGTCCGGTGC CCTGAATGAA CTGCAGGACG AGGCAGCGCG 10621 GCTATCGTGG CTGGCCACGA CGGGCGTTCC TTGCGCAGCT GTGCTCGACG TTGTCACTGA 10681 AGCGGGAAGG GACTGGCTGC TATTGGGCGA AGTGCCGGGG CAGGATCTCC TGTCATCTCA 10741 CCTTGCTCCT GCCGAGAAAG TATCCATCAT GGCTGATGCA ATGCGGCGGC TGCATACGCT 10801 TGATCCGGCT ACCTGCCCAT TCGACCACCA AGCGAAACAT CGCATCGAGC GAGCACGTAC 10861 TCGGATGGAA GCCGGTCTTG TCGATCAGGA TGATCTGGAC GAAGAGCATC AGGGGCTCGC 10921 GCCAGCCGAA CTGTTCGCCA GGCTCAAGGC GCGCATGCCC GACGGCGAGG ATCTCGTCGT 10981 GACCCATGGC GATGCCTGCT TGCCGAATAT CATGGTGGAA AATGGCCGCT TTTCTGGATT 11041 CATCGACTGT GGCCGGCTGG GTGTGGCGGA CCGCTATCAG GACATAGCGT TGGCTACCCG 11101 TGATATTGCT GAAGAGCTTG GCGGCGAATG GGCTGACCGC TTCCTCGTGC TTTACGGTAT 11161 CGCCGCTCCC GATTCGCAGC GCATCGCCTT CTATCGCCTT CTTGACGAGT TCTTCTGAGC 11221 GGGACTCTGG GGTTCGCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT 11281 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG 11341 GCTGGATGAT CCTCCAGCGC GGGGATCTCA TGCTGGAGTT CTTCGCCCAC CCCAACTTGT 11401 TTATTGCAGC TTATAATGGT TACAAATAAA GCAATAGCAT CACAAATTTC ACAAATAAAG 11461 CATTTTTTTC ACTGCATTCT AGTTGTGGTT TGTCCAAACT CATCAATGTA TCTTATCATG 11521 TCTGTATACC GTCGACCTCT AGCTAGAGCT TGGCGTAATC ATGGTCATAG CTGTTTCCTG 11581 TGTGAAATTG TTATCCGCTC ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA 11641 AAGCCTGGGG TGCCTAATGA GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG 11701 CTTTCCAGTC GGGAAACCTG TCGTGCCAGC TGCATTAATG AATCGGCCAA CGCGCGGGGA 11761 GAGGCGGTTT GCGTATTGGG CGCTCTTCCG CTTCCTCGCT CACTGACTCG CTGCGCTCGG 11821 TCGTTCGGCT GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 11881 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 11941 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 12001 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 12061 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 12121 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA ATGCTCACGC TGTAGGTATC 12181 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 12241 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 12301 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 12361 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGGACA GTATTTGGTA 12421 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 12481 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 12541 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 12601 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 12661 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA ACTTGGTCTG 12721 ACAGTTACCA ATGCTTAATC AGTGAGGCAC CTATCTCAGC GATCTGTCTA TTTCGTTCAT 12781 CCATAGTTGC CTGACTCCCC GTCGTGTAGA TAACTACGAT ACGGGAGGGC TTACCATCTG 12841 GCCCCAGTGC TGCAATGATA CCGCGAGACC CACGCTCACC GGCTCCAGAT TTATCAGCAA 12901 TAAACCAGCC AGCCGGAAGG GCCGAGCGCA GAAGTGGTCC TGCAACTTTA TCCGCCTCCA 12961 TCCAGTCTAT TAATTGTTGC CGGGAAGCTA GAGTAAGTAG TTCGCCAGTT AATAGTTTGC 13021 GCAACGTTGT TGCCATTGCT ACAGGCATCG TGGTGTCACG CTCGTCGTTT GGTATGGCTT 13081 CATTCAGCTC CGGTTCCCAA CGATCAAGGC GAGTTACATG ATCCCCCATG TTGTGCAAAA 13141 AAGCGGTTAG CTCCTTCGGT CCTCCGATCG TTGTCAGAAG TAAGTTGGCC GCAGTGTTAT 13201 CACTCATGGT TATGGCAGCA CTGCATAATT CTCTTACTGT CATGCCATCC GTAAGATGCT 13261 TTTCTGTGAC TGGTGAGTAC TCAACCAAGT CATTCTGAGA ATAGTGTATG CGGCGACCGA 13321 GTTGCTCTTG CCCGGCGTCA ATACGGGATA ATACCGCGCC ACATAGCAGA ACTTTAAAAG 13381 TGCTCATCAT TGGAAAACGT TCTTCGGGGC GAAAACTCTC AAGGATCTTA CCGCTGTTGA 13441 GATCCAGTTC GATGTAACCC ACTCGTGCAC CCAACTGATC TTCAGCATCT TTTACTTTCA 13501 CCAGCGTTTC TGGGTGAGCA AAAACAGGAA GGCAAAATGC CGCAAAAAAG GGAATAAGGG 13561 CGACACGGAA ATGTTGAATA CTCATACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 13621 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 13681 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGT CGACGGATCG GGAGATCTCC 13741 CGATCCCCTA TGGTCGACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA 13801 TCTGCTCCCT GCTTGTGTGT TGGAGGTCGC TGAGTAGTGC GCGAGCAAAA TTTAAGCTAC 13861 AACAAGGCAA GGCTTGACCG ACAATTGCAT GAAGAATCTG CTTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.