pLV3-CMV-SELENOT-3×FLAG-SECIS-

pLV3-CMV-SELENOT-3×FLAG-SECIS-CopGFP-Puro plasmid

PVT95094

pLV3-CMV-SELENOT-3×FLAG-SECIS-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-SELENOT(human)-3×FLAG-SECIS-CopGFP-Puro
Alias: NM_016275.5;SELT
Gene lenght: 585bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-SELENOT-3×FLAG-SECIS-CopGFP-Puro plasmid Description

pLV3-CMV-SELENOT-3×FLAG-SECIS-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-SELENOT-3×FLAG-SECIS-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatga ggcttctgct gcttctccta 2341 gtggcggcgt ctgcgatggt ccggagcgag gcctcggcca atctgggcgg cgtgcccagc 2401 aagagattaa agatgcagta cgccacgggg ccgctgctca agttccagat ttgtgtttcc 2461 tgaggttata ggcgggtgtt tgaggagtac atgcgggtta ttagccagcg gtacccagac 2521 atccgcattg aaggagagaa ttacctccct caaccaatat atagacacat agcatctttc 2581 ctgtcagtct tcaaactagt attaataggc ttaataattg ttggcaagga tccttttgct 2641 ttctttggca tgcaagctcc tagcatctgg cagtggggcc aagaaaataa ggtttatgca 2701 tgtatgatgg ttttcttctt gagcaacatg attgagaacc agtgtatgtc aacaggtgca 2761 tttgagataa ctttaaatga tgtacctgtg tggtctaagc tggaatctgg tcaccttcca 2821 tccatgcaac aacttgttca aattcttgac aatgaaatga agctcaatgt gcatatggat 2881 tcaatcccac accatcgatc aggatccGAC TACAAAGACC ATGACGGTGA TTATAAAGAT 2941 CATGACATCG ACTACAAGGA TGACGATGAC AAGtaggtga ctgacattat gaaggcctgt 3001 actgaagaca gcaagctgtt agtacagacc agatgctttc ttggcaggct cggatccCTG 3061 CGATATCCAG CgcggccgcG aaggatctgc gatcgctccg gtgcccgtca gtgggcagag 3121 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc 3181 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 3241 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 3301 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc 3361 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc 3421 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga 3481 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt 3541 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac 3601 agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc 3661 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag 3721 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc 3781 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc 3841 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac 3901 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc 3961 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc 4021 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag 4081 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg 4141 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc 4201 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg 4261 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc 4321 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc 4381 tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat 4441 cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc 4501 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc 4561 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 4621 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 4681 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 4741 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg 4801 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 4861 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc 4921 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 4981 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 5041 ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 5101 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 5161 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 5221 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 5281 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 5341 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 5401 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca 5461 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 5521 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 5581 ccgcgtctcc gccttcgccc tcagacgagt cggatctccc tttggccgcc tccccgcctg 5641 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa 5701 aaggggggac tggaagggct aattcactcc caacgaaaat aagatctgct ttttgcttgt 5761 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 5821 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 5881 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 5941 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata 6001 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc 6061 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa 6121 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt 6181 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg 6241 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagactttt 6301 gcagagacgg cccaaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc 6361 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct 6421 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa 6481 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 6541 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 6601 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg 6661 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt 6721 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa 6781 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct 6841 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc 6901 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg 6961 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct 7021 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag 7081 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga 7141 agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga 7201 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg 7261 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag 7321 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag 7381 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat 7441 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct 7501 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac 7561 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa 7621 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg 7681 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt 7741 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca 7801 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt 7861 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct 7921 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg 7981 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg 8041 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg 8101 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa 8161 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt 8221 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt 8281 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt 8341 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca 8401 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat 8461 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata 8521 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc 8581 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca 8641 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg 8701 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat 8761 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg 8821 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg 8881 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg 8941 ccagtgccaa gctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.