pCMV-Nos2-3×Myc-Neo plasmid

pCMV-Nos2-3×Myc-Neo plasmid

PVT95090

pCMV-Nos2-3×Myc-Neo plasmid Information

Plasmid name: pCMV-Nos2(mouse)-3×Myc-Neo
Alias: NM_010927.4;i-NOS; iNOS; MAC-NOS; Nos-2; NOS-II; Nos2a
Gene lenght: 3432bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Nos2-3×Myc-Neo plasmid Description

pCMV-Nos2-3×Myc-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Nos2-3×Myc-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatggc ttgcccctgg 721 aagtttctct tcaaagtcaa atcctaccaa agtgacctga aagaggaaaa ggacattaac 781 aacaacgtga agaaaacccc ttgtgctgtt ctcagcccaa caatacaaga tgaccctaag 841 agtcaccaaa atggctcccc gcagctcctc actgggacag cacagaatgt tccagaatcc 901 ctggacaagc tgcatgtgac atcgacccgt ccacagtatg tgaggatcaa aaactggggc 961 agtggagaga ttttgcatga cactcttcac cacaaggcca catcggattt cacttgcaag 1021 tccaagtctt gcttggggtc catcatgaac cccaagagtt tgaccagagg acccagagac 1081 aagcctaccc ctctggagga gctcctgcct catgccattg agttcatcaa ccagtattat 1141 ggctccttta aagaggcaaa aatagaggaa catctggcca ggctggaagc tgtaacaaag 1201 gaaatagaaa caacaggaac ctaccagctc actctggatg agctcatctt tgccaccaag 1261 atggcctgga ggaatgcccc tcgctgcatc ggcaggatcc agtggtccaa cctgcaggtc 1321 tttgacgctc ggaactgtag cacagcacag gaaatgtttc agcacatctg cagacacata 1381 ctttatgcca ccaacaatgg caacatcagg tcggccatca ctgtgttccc ccagcggagt 1441 gacggcaaac atgacttcag gctctggaat tcacagctca tccggtacgc tggctaccag 1501 atgcccgatg gcaccatcag aggggatgct gccaccttgg agttcaccca gttgtgcatc 1561 gacctaggct ggaagccccg ctatggccgc tttgatgtgc tgcctctggt cttgcaagct 1621 gatggtcaag atccagaggt ctttgaaatc cctcctgatc ttgtgttgga ggtgaccatg 1681 gagcatccca agtacgagtg gttccaggag ctcgggttga agtggtatgc actgcctgcc 1741 gtggccaaca tgctactgga ggtgggtggc ctcgaattcc cagcctgccc cttcaatggt 1801 tggtacatgg gcaccgagat tggagttcga gacttctgtg acacacagcg ctacaacatc 1861 ctggaggaag tgggccgaag gatgggcctg gagacccaca cactggcctc cctctggaaa 1921 gaccgggctg tcacggagat caatgtggct gtgctccata gtttccagaa gcagaatgtg 1981 accatcatgg accaccacac agcctcagag tccttcatga agcacatgca gaatgagtac 2041 cgggcccgtg gaggctgccc ggcagactgg atttggctgg tccctccagt gtctgggagc 2101 atcacccctg tgttccacca ggagatgttg aactatgtcc tatctccatt ctactactac 2161 cagatcgagc cctggaagac ccacatctgg cagaatgaga agctgaggcc caggaggaga 2221 gagatccgat ttagagtctt ggtgaaagtg gtgttctttg cttccatgct aatgcgaaag 2281 gtcatggctt cacgggtcag agccacagtc ctctttgcta ctgagacagg gaagtctgaa 2341 gcactagcca gggacctggc caccttgttc agctacgcct tcaacaccaa ggttgtctgc 2401 atggaccagt ataaggcaag caccttggaa gaggagcaac tactgctggt ggtgacaagc 2461 acatttggga atggagactg tcccagcaat gggcagactc tgaagaaatc tctgttcatg 2521 cttagagaac tcaaccacac cttcaggtat gctgtgtttg gccttggctc cagcatgtac 2581 cctcagttct gcgcctttgc tcatgacatc gaccagaagc tgtcccacct gggagcctct 2641 cagcttgccc caacaggaga aggggacgaa ctcagtgggc aggaggatgc cttccgcagc 2701 tgggctgtac aaaccttccg ggcagcctgt gagacctttg atgtccgaag caaacatcac 2761 attcagatcc cgaaacgctt cacttccaat gcaacatggg agccacagca atataggctc 2821 atccagagcc cggagccttt agacctcaac agagccctca gcagcatcca tgcaaagaac 2881 gtgtttacca tgaggctgaa atcccagcag aatctgcaga gtgaaaagtc cagccgcacc 2941 accctcctcg ttcagctcac cttcgagggc agccgagggc ccagctacct gcctggggaa 3001 cacctgggga tcttcccagg caaccagacc gccctggtgc agggaatctt ggagcgagtt 3061 gtggattgtc ctacaccaca ccaaactgtg tgcctggagg ttctggatga gagcggcagc 3121 tactgggtca aagacaagag gctgcccccc tgctcactca gccaagccct cacctacttc 3181 ctggacatta cgacccctcc cacccagctg cagctccaca agctggctcg ctttgccacg 3241 gacgagacgg ataggcagag attggaggcc ttgtgtcagc cctcagagta caatgactgg 3301 aagttcagca acaaccccac gttcctggag gtgcttgaag agttcccttc cttgcatgtg 3361 cccgctgcct tcctgctgtc gcagctccct atcttgaagc cccgctacta ctccatcagc 3421 tcctcccagg accacacccc ctcggaggtt cacctcactg tggccgtggt cacctaccgc 3481 acccgagatg gtcagggtcc cctgcaccat ggtgtctgca gcacttggat caggaacctg 3541 aagccccagg acccagtgcc ctgctttgtg cgaagtgtca gtggcttcca gctccctgag 3601 gacccctccc agccttgcat cctcattggg cctggtacgg gcattgctcc cttccgaagt 3661 ttctggcagc agcggctcca tgactcccag cacaaagggc tcaaaggagg ccgcatgagc 3721 ttggtgtttg ggtgccggca cccggaggag gaccacctct atcaggaaga aatgcaggag 3781 atggtccgca agagagtgct gttccaggtg cacacaggct actcccggct gcccggcaaa 3841 cccaaggtct acgttcagga catcctgcaa aagcagctgg ccaatgaggt actcagcgtg 3901 ctccacgggg agcagggcca cctctacatt tgcggagatg tgcgcatggc tcgggatgtg 3961 gctaccacat tgaagaagct ggtggccacc aagctgaact tgagcgagga gcaggtggaa 4021 gactatttct tccagctcaa gagccagaaa cgttatcatg aagatatctt cggtgcagtc 4081 ttttcctatg gggcaaaaaa gggcagcgcc ttggaggagc ccaaagccac gaggctcCTC 4141 GAGGAACAAA AACTAATATC AGAGGAAGAT TTGGAACAGA AACTAATCAG TGAAGAAGAT 4201 TTAGAGCAAA AGTTAATTTC CGAGGAGGAC TTATAGTGAG GGCCCGATAT CTCGACAATC 4261 AACCTCTGGA TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT 4321 TTACGCTATG TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG 4381 CTTTCATTTT CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC 4441 CCGTTGTCAG GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT 4501 GGGGCATTGC CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG 4561 CCACGGCGGA ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG 4621 GCACTGACAA TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG CTGCTCGCCT 4681 GTGTTGCCAC CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC 4741 CAGCGGACCT TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC 4801 TTCGCCCTCA GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCCTGGA AACGGGGGAG 4861 GCTAACTGAA ACACGGAAGG AGACAATACC GGAAGGAACC CGCGCTATGA CGGCAATAAA 4921 AAGACAGAAT AAAACGCACG GGTGTTGGGT CGTTTGTTCA TAAACGCGGG GTTCGGTCCC 4981 AGGGCTGGCA CTCTGTCGAT ACCCCACCGA GACCCCATTG GGGCCAATAC GCCCGCGTTT 5041 CTTCCTTTTC CCCACCCCAC CCCCCAAGTT CGGGTGAAGG CCCAGGGCTC GCAGCCAACG 5101 TCGGGGCGGC AGGCCCTGCC ATAGCAGATC TGCGCAGCTG GGGCTCTAGG GGGTATCCCC 5161 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG 5221 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA 5281 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGCAT CCCTTTAGGG TTCCGATTTA 5341 GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC 5401 CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG 5461 GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT 5521 AAGGGATTTT GGGGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA 5581 ACGCGAATTA ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC 5641 AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC 5701 CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT 5761 AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC 5821 GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA 5881 GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC 5941 GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT 6001 GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG 6061 CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC 6121 GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA 6181 GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT 6241 CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA 6301 TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG 6361 GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT 6421 CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA 6481 GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG 6541 CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG 6601 CCGCTTTTCT GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT 6661 AGCGTTGGCT ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT 6721 CGTGCTTTAC GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA 6781 CGAGTTCTTC TGAGCGGGAC TCTGGGGTTC GCGAAATGAC CGACCAAGCG ACGCCCAACC 6841 TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG 6901 TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG 6961 CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA 7021 ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA 7081 ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT 7141 CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG 7201 GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT 7261 TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG 7321 GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG 7381 ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA 7441 TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC 7501 AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC 7561 CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT 7621 AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC 7681 CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT 7741 CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG 7801 AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC 7861 CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA 7921 GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA 7981 GGACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA 8041 GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC 8101 AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG 8161 ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA 8221 TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG 8281 AGTAAACTTG GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT 8341 GTCTATTTCG TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG 8401 AGGGCTTACC ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC 8461 CAGATTTATC AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA 8521 CTTTATCCGC CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC 8581 CAGTTAATAG TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT 8641 CGTTTGGTAT GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC 8701 CCATGTTGTG CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT 8761 TGGCCGCAGT GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC 8821 CATCCGTAAG ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT 8881 GTATGCGGCG ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA 8941 GCAGAACTTT AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA 9001 TCTTACCGCT GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG 9061 CATCTTTTAC TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA 9121 AAAAGGGAAT AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT 9181 ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA 9241 AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCGACG 9301 GATCGGGAGA TCTCCCGATC CCCTATGGTC GACTCTCAGT ACAATCTGCT CTGATGCCGC 9361 ATAGTTAAGC CAGTATCTGC TCCCTGCTTG TGTGTTGGAG GTCGCTGAGT AGTGCGCGAG 9421 CAAAATTTAA GCTACAACAA GGCAAGGCTT GACCGACAAT TGCATGAAGA ATCTGCTTAG 9481 G

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.