pLV2-CMV-SNCA-EGFP-Puro plasmi

pLV2-CMV-SNCA-EGFP-Puro plasmid

PVT95086

pLV2-CMV-SNCA-EGFP-Puro plasmid Information

Plasmid name: pLV2-CMV-SNCA(human)-EGFP-Puro
Alias: NM_000345.4;NACP; PARK1; PARK4; PD1
Gene lenght: 420bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-SNCA-EGFP-Puro plasmid Description

pLV2-CMV-SNCA-EGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-CMV-SNCA-EGFP-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCatggatgt attcatgaaa ggactttcaa aggccaagga 2881 gggagttgtg gctgctgctg agaaaaccaa acagggtgtg gcagaagcag caggaaagac 2941 aaaagagggt gttctctatg taggctccaa aaccaaggag ggagtggtgc atggtgtggc 3001 aacagtggct gagaagacca aagagcaagt gacaaatgtt ggaggagcag tggtgacggg 3061 tgtgacagca gtagcccaga agacagtgga gggagcaggg agcattgcag cagccactgg 3121 ctttgtcaaa aaggaccagt tgggcaagaa tgaagaagga gccccacagg aaggaattct 3181 ggaagatatg cctgtggatc ctgacaatga ggcttatgaa atgccttctg aggaagggta 3241 tcaagactac gaacctgaag ccggtggagg cggatcagga ggcggtggat ctggatccgc 3301 caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct 3361 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac 3421 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc 3481 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat 3541 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat 3601 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac 3661 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg 3721 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa 3781 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct 3841 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa 3901 ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat 3961 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa 4021 gtagtctaga taattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg 4081 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca 4141 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc 4201 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac 4261 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca 4321 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg 4381 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg 4441 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc 4501 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc 4561 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 4621 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 4681 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 4741 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 4801 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 4861 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 4921 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 4981 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 5041 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 5101 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 5161 ccgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct 5221 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc 5281 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct 5341 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga 5401 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc 5461 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac 5521 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt 5581 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt 5641 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc 5701 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc 5761 gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat 5821 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt 5881 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 5941 agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa 6001 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac 6061 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca 6121 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct 6181 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc 6241 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga 6301 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc 6361 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac 6421 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc 6481 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt 6541 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag 6601 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc 6661 agagagtgag aggccttgac attgctagcg ttttaccgtc gacctctagc tagagcttgg 6721 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 6781 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca 6841 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 6901 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 6961 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 7021 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 7081 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 7141 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 7201 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 7261 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 7321 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 7381 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 7441 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 7501 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 7561 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 7621 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 7681 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 7741 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 7801 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 7861 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 7921 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 7981 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 8041 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 8101 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 8161 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 8221 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 8281 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 8341 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 8401 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 8461 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 8521 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 8581 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 8641 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 8701 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 8761 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 8821 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 8881 ctgacgtcga cggatcggga gatcaacttg tttattgcag cttataatgg ttacaaataa 8941 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt 9001 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac 9061 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt 9121 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt 9181 taaatatgta ctacaaactt agtagttttt aaagaaattg tatttgttaa atatgtacta 9241 caaacttagt agt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.