pCMV-FBXW10-3×FLAG-Neo plasmid

pCMV-FBXW10-3×FLAG-Neo plasmid

PVT95073

pCMV-FBXW10-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-FBXW10(human)-3×FLAG-Neo
Alias: NM_001267585.2;Fbw10; HREP; SM25H2; SM2SH2
Gene lenght: 3156bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-FBXW10-3×FLAG-Neo plasmid Description

pCMV-FBXW10-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-FBXW10-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgga aaacctggaa tcaaggctca 781 agaatgcccc ctattttcgt tgtgagaagg gaaccgattc catccctcta tgccggaagt 841 gtgagacgtg tgtcttagcc tggaagatct tctctaccaa agagtggttc tgcaggatca 901 atgacatatc acagaggagg tttctagttg gcattctgaa gcagttaaat agcttatatt 961 tgttacacta tttccaaaat atccttcaga ccacacaggg aaaggatttc atctataaca 1021 ggtcccggat caacctcagc aagaaagagg ggaaagttgt gaagtcctcc ttgaaccaaa 1081 tgttggataa aacagtagaa cagaagatga aagagatctt gtactggttt gcgaacagca 1141 cccagtggac caaggcgaat tatactctct tactgctgca gatgtgcaac cccaaattac 1201 tgctcactgc tgccaatgtg atcagagtcc tgtttctgag agaggagaac aatatctcag 1261 ggctcaatca agacatcaca gatgtgtgtt tttcccctga gaaagaccac agctccaagt 1321 ctgcgacctc acaagtctat tggacagcca aaactcagca cacatccctt cctttgtcca 1381 aagccccaga aaatgaacac ttgcttgggg cagcatctaa ccctgaggaa ccatggagga 1441 attcactccg gtgtatatcc gaaatgaata ggctgttttc tggaaaagga gacataacca 1501 agccagggta cgatccctgc aatctattgg ttgacctgga tgacatcaga gacctgtctt 1561 ctgggttcag caaataccga gacttcatcc gttacctgcc catccacctc tccaagtaca 1621 ttctaagaat gctggataga cacaccctga acaagtgcgc ctctgtgagc cagcactggg 1681 ccgccatggc tcaacaggtc aagatggact tgtcagcgca cggcttcatt cagaaccaga 1741 ttaccttctt gcaggggtcc tacacaagag gaattgatcc taattatgcc aataaggttt 1801 ctatcccagt tcctaaaatg gtagatgacg ggaagagcat gcgtgtgaaa catccgaagt 1861 ggaagctgag aacgaagaat gagtacaacc tgtggactgc ataccagaac gaggaaacgc 1921 agcaggtcct gatagaggag agaaatgttt tctgtgggac ctacaatgtt cgcattctct 1981 ctgacacgtg ggatcaaaac cgagtcatcc actattccgg gggagatctg atagctgtgt 2041 catctaatcg aaagatccat cttctggaca tcatacaagt gaaagcgata cccgttgaat 2101 tccgaggcca tgctgggagt gtccgggccc tcttcctgtg tgaggaggaa aactttctcc 2161 taagcgggag ctatgaccta agtatcagat actgggatct gaaaagtggg gtttgcacac 2221 gaatcttcgg tggtcaccag gggactatca cttgcatgga cttgtgtaag aacaggctcg 2281 tatctggagg aagagattgc caggtaaaag tatgggatgt agacacaggg aagtgcctga 2341 agacgtttag acacaaagac cccatcttgg ccaccaggat caatgatacc tacattgtga 2401 gcagctgtga gcgagggctg gtgaaagtgt ggcacattgc catggcccag ttggtaaaga 2461 ctctcagtgg ccatgaggga gccgtgaaat gcctgttctt tgaccagtgg catctcctct 2521 caggaagtac tgatggcctg gtcatggcct ggagcatggt ggggaagtac gagcgctgcc 2581 tgatggcctt caagcatccc aaggaggtgc tcgacgtgtc ccttctcttc ctccgggtca 2641 tcagcgcctg tgcagatggc aagatccgaa tttacaattt cctcaacggg aactgtatga 2701 aggtgttaaa agccaatggc agaggtgatc ctgtgctgtc cttctttatt cagggcaaca 2761 ggatggtggt caacacagag agcaatgttc tcatgttcca gtttgagcac ataaagtggc 2821 agtatgccgt ggaaaaaacg aaacaaaaga agaataagga gaaagaggag gaaaaagaag 2881 aaaatagtct catggaaatt ctctctaagt gtaatattca ggttcacagc ccaagagagt 2941 ctgtatccag taaacaaact gtgatccaag agctcctacc aggcaaacct cccaagtccc 3001 gagtactcct gaagccggcc aagttctctt cagcagtgtt aatagaggaa cttcaaagtc 3061 aaggaaagtc aaaatcaccc cgaagagatg cagatgatgt ggagaaagca caaaaacaag 3121 gacaattgga aactcctgga aaactgccca gtcacccaaa gaaaaagtct tggaaaatcc 3181 ctatgtcacc tgaccaattc ctcctgactg ttagcgccct gcagcacgcc cataattccg 3241 gggaatttgc ctatccctgt aggccccaaa cagaaattac tgatgtctgg ggaccttcaa 3301 tttcataccc aaggaaggtc ttgaatttca aaggaaaatc aatccaacgt gcagttgatc 3361 ggttgagatt gagcaatcct cctatagatg tgaaacgaac cagtattccc cttgaaatcc 3421 agaaactgca gcccaacttg aagatctctt tgcacagtcc tagagtccag tccaccatac 3481 cccagcccat gattatccgc tccaggttct ctggcagctt aaagggtgga gaccaagtga 3541 ccagttcaat tgaaagggct gtgtgcagta cgggtcccct gaccagtatg caggtcatta 3601 aaccaaaccg catgctagct ccacaagtgg gcacagccac cctgtctctt aagaaagaac 3661 ggcctcgcat ctatacagcc cttgatcctt ttagagtgaa cactgagttc gtgctgttga 3721 ccgtgaagga ggagaaggag caccaggaag ccaagatgaa ggaatatcag gccagggagt 3781 ccactggagt ggttgatcca ggaaaagtca gcaaagctgc atggatcagg aagatcaaag 3841 gcctgcctat tgataatttc acgaagcaag ggaaaacagc ggcccctgaa cttggacaaa 3901 atgtatttat cGGATCCGAC TACAAAGACC ATGACGGTGA TTATAAAGAT CATGACATCG 3961 ACTACAAGGA TGACGATGAC AAGTAGTGAG GGCCCGATAT CTCGACAATC AACCTCTGGA 4021 TTACAAAATT TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG 4081 TGGATACGCT GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT 4141 CTCCTCCTTG TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG 4201 GCAACGTGGC GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC 4261 CACCACCTGT CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA 4321 ACTCATCGCC GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA 4381 TTCCGTGGTG TTGTCGGGGA AGCTGACGTC CTTTCCATGG CTGCTCGCCT GTGTTGCCAC 4441 CTGGATTCTG CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT 4501 TCCTTCCCGC GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA 4561 GACGAGTCGG ATCTCCCTTT GGGCCGCCTC CCCGCCTGGA AACGGGGGAG GCTAACTGAA 4621 ACACGGAAGG AGACAATACC GGAAGGAACC CGCGCTATGA CGGCAATAAA AAGACAGAAT 4681 AAAACGCACG GGTGTTGGGT CGTTTGTTCA TAAACGCGGG GTTCGGTCCC AGGGCTGGCA 4741 CTCTGTCGAT ACCCCACCGA GACCCCATTG GGGCCAATAC GCCCGCGTTT CTTCCTTTTC 4801 CCCACCCCAC CCCCCAAGTT CGGGTGAAGG CCCAGGGCTC GCAGCCAACG TCGGGGCGGC 4861 AGGCCCTGCC ATAGCAGATC TGCGCAGCTG GGGCTCTAGG GGGTATCCCC ACGCGCCCTG 4921 TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC 4981 CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG 5041 CTTTCCCCGT CAAGCTCTAA ATCGGGGCAT CCCTTTAGGG TTCCGATTTA GTGCTTTACG 5101 GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG 5161 ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT 5221 CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT 5281 GGGGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTA 5341 ATTCTGTGGA ATGTGTGTCA GTTAGGGTGT GGAAAGTCCC CAGGCTCCCC AGCAGGCAGA 5401 AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT GTGGAAAGTC CCCAGGCTCC 5461 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAT AGTCCCGCCC 5521 CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG CCCATTCTCC GCCCCATGGC 5581 TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT CTGCCTCTGA GCTATTCCAG 5641 AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA AAAAGCTCCC GGGAGCTTGT 5701 ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA 5761 GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG 5821 GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC 5881 CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA 5941 GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC 6001 ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA 6061 TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT 6121 ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA 6181 CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG 6241 CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC 6301 GTCGTGACCC ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT 6361 GGATTCATCG ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT 6421 ACCCGTGATA TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC 6481 GGTATCGCCG CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC 6541 TGAGCGGGAC TCTGGGGTTC GCGAAATGAC CGACCAAGCG ACGCCCAACC TGCCATCACG 6601 AGATTTCGAT TCCACCGCCG CCTTCTATGA AAGGTTGGGC TTCGGAATCG TTTTCCGGGA 6661 CGCCGGCTGG ATGATCCTCC AGCGCGGGGA TCTCATGCTG GAGTTCTTCG CCCACCCCAA 6721 CTTGTTTATT GCAGCTTATA ATGGTTACAA ATAAAGCAAT AGCATCACAA ATTTCACAAA 6781 TAAAGCATTT TTTTCACTGC ATTCTAGTTG TGGTTTGTCC AAACTCATCA ATGTATCTTA 6841 TCATGTCTGT ATACCGTCGA CCTCTAGCTA GAGCTTGGCG TAATCATGGT CATAGCTGTT 6901 TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA 6961 GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT 7021 GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC 7081 GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC TTCCGCTTCC TCGCTCACTG ACTCGCTGCG 7141 CTCGGTCGTT CGGCTGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 7201 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 7261 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 7321 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 7381 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 7441 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCAATGCT CACGCTGTAG 7501 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 7561 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 7621 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 7681 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GGACAGTATT 7741 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 7801 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 7861 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 7921 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 7981 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAAACTTG 8041 GTCTGACAGT TACCAATGCT TAATCAGTGA GGCACCTATC TCAGCGATCT GTCTATTTCG 8101 TTCATCCATA GTTGCCTGAC TCCCCGTCGT GTAGATAACT ACGATACGGG AGGGCTTACC 8161 ATCTGGCCCC AGTGCTGCAA TGATACCGCG AGACCCACGC TCACCGGCTC CAGATTTATC 8221 AGCAATAAAC CAGCCAGCCG GAAGGGCCGA GCGCAGAAGT GGTCCTGCAA CTTTATCCGC 8281 CTCCATCCAG TCTATTAATT GTTGCCGGGA AGCTAGAGTA AGTAGTTCGC CAGTTAATAG 8341 TTTGCGCAAC GTTGTTGCCA TTGCTACAGG CATCGTGGTG TCACGCTCGT CGTTTGGTAT 8401 GGCTTCATTC AGCTCCGGTT CCCAACGATC AAGGCGAGTT ACATGATCCC CCATGTTGTG 8461 CAAAAAAGCG GTTAGCTCCT TCGGTCCTCC GATCGTTGTC AGAAGTAAGT TGGCCGCAGT 8521 GTTATCACTC ATGGTTATGG CAGCACTGCA TAATTCTCTT ACTGTCATGC CATCCGTAAG 8581 ATGCTTTTCT GTGACTGGTG AGTACTCAAC CAAGTCATTC TGAGAATAGT GTATGCGGCG 8641 ACCGAGTTGC TCTTGCCCGG CGTCAATACG GGATAATACC GCGCCACATA GCAGAACTTT 8701 AAAAGTGCTC ATCATTGGAA AACGTTCTTC GGGGCGAAAA CTCTCAAGGA TCTTACCGCT 8761 GTTGAGATCC AGTTCGATGT AACCCACTCG TGCACCCAAC TGATCTTCAG CATCTTTTAC 8821 TTTCACCAGC GTTTCTGGGT GAGCAAAAAC AGGAAGGCAA AATGCCGCAA AAAAGGGAAT 8881 AAGGGCGACA CGGAAATGTT GAATACTCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT 8941 TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA 9001 AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGTCGACG GATCGGGAGA 9061 TCTCCCGATC CCCTATGGTC GACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC 9121 CAGTATCTGC TCCCTGCTTG TGTGTTGGAG GTCGCTGAGT AGTGCGCGAG CAAAATTTAA 9181 GCTACAACAA GGCAAGGCTT GACCGACAAT TGCATGAAGA ATCTGCTTAG G

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.