pLV3-CMV-HERC5-3×FLAG-CopGFP-P

pLV3-CMV-HERC5-3×FLAG-CopGFP-Puro plasmid

PVT95064

pLV3-CMV-HERC5-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-HERC5(human)-3×FLAG-CopGFP-Puro
Alias: NM_016323.4;CEB1; CEBP1;
Gene lenght: 3072bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pLV3-CMV-HERC5-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-HERC5-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-HERC5-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCatgg agcggaggtc gcggaggaag 2341 tcgcggcgca acgggcgctc gaccgcgggc aaggccgccg cgacccagcc cgcgaagtct 2401 ccgggcgcac agctctggct ctttcccagc gccgcgggcc tccaccgcgc gctgctccgg 2461 agggtggagg tgacgcgcca actctgctgc tcgccggggc gcctcgcggt cttggaacgc 2521 ggcggggcgg gcgtccaggt tcaccagctg ctcgccggga gcggcggcgc ccggacgccg 2581 aaatgcatta aattaggaaa aaacatgaag atacattccg tggaccaagg agcagagcac 2641 atgctgattc tctcatcaga tggaaaacca tttgagtatg acaactatag catgaaacat 2701 ctaaggtttg aaagcatttt acaagaaaaa aaaataattc agatcacatg tggagattac 2761 cattctcttg cactctcaaa aggtggtgag ctttttgcct ggggacagaa cctgcatggg 2821 cagcttggag ttggaaggaa atttccctca accaccacac cacagattgt ggagcacctc 2881 gcaggagtac ccttggctca gatttctgcc ggagaagccc acagcatggc cttatccatg 2941 tctggcaaca tttattcatg gggaaaaaat gaatgtggac aactaggcct gggccacact 3001 gagagtaaag atgatccatc ccttattgaa ggactagaca atcagaaagt tgaatttgtc 3061 gcttgtggtg gctctcacag tgccctactc acacaggatg ggctgctgtt tactttcggt 3121 gctggaaaac atgggcaact tggtcataat tcaacacaga atgagctaag accctgtttg 3181 gtggctgagc ttgttgggta tagagtgact cagatagcat gtggaaggtg gcacacactt 3241 gcctatgttt ctgatttggg aaaggtcttt tcctttggtt ctggaaaaga tggacaactg 3301 ggaaatggtg gaacacgtga ccagctgatg ccgcttccag tgaaagtatc atcaagtgaa 3361 gaactcaaac ttgaaagcca tacctcagaa aaggagttaa taatgattgc tggagggaat 3421 caaagcattt tgctctggat aaagaaagag aattcatatg ttaatctgaa gaggacaatt 3481 cctactctga atgaagggac tgtaaagaga tggattgctg atgtggagac taaacggtgg 3541 cagagcacaa aaagggaaat ccaagagata ttttcatctc ctgcttgtct aactggaagt 3601 tttttaagga aaagaagaac tacagaaatg atgcctgttt atttggactt aaataaagca 3661 agaaacatct tcaaggagtt aacccaaaag gactggatta ctaacatgat aaccacctgc 3721 ctcaaagata atctgctcaa aagacttcca tttcattctc caccccaaga agctttagaa 3781 attttcttcc ttctcccaga atgtcctatg atgcatattt ccaacaactg ggagagcctt 3841 gtggttccat ttgcaaaggt tgtttgtaaa atgagtgacc agtcttcact ggttctggaa 3901 gagtattggg caactctgca agaatccact ttcagcaaac tggtccagat gtttaaaaca 3961 gccgtcatat gccagttgga ttactgggat gaaagtgctg aggagaatgg taatgttcaa 4021 gctctcctag aaatgttgaa gaagctgcac agggtaaacc aggtgaaatg tcaactacct 4081 gaaagtattt tccaagtaga cgaactcttg caccgtctca atttttttgt agaagtatgc 4141 agaaggtact tgtggaaaat gactgtggac gcttcagaaa atgtacaatg ctgcgtcata 4201 ttcagtcact ttccatttat ctttaataat ctgtcgaaaa ttaaactact acatacagac 4261 acacttttaa aaatagagag taaaaaacat aaagcttatc ttaggtcggc agcaattgag 4321 gaagaaagag agtctgaatt cgctttgagg cccacgtttg atctaacagt cagaaggaat 4381 cacttgattg aggatgtttt gaatcagcta agtcaatttg agaatgaaga cctgaggaaa 4441 gagttatggg tttcatttag tggagaaatt gggtatgacc tcggaggagt caagaaagag 4501 ttcttctact gtctgtttgc agagatgatc cagccggaat atgggatgtt catgtatcct 4561 gaaggggctt cctgcatgtg gtttcctgtc aagcctaaat ttgagaagaa aagatacttc 4621 ttttttgggg ttctatgtgg actttccctg ttcaattgca atgttgccaa ccttcctttc 4681 ccactggcac tgtttaagaa acttttggac caaatgccat cattggaaga cttgaaagaa 4741 ctcagtcctg atttgggaaa gaatttgcaa acacttctgg atgatgaagg tgataacttt 4801 gaggaagtat tttacatcca ttttaatgtg cactgggaca gaaacgacac aaacttaatt 4861 cctaatggaa gtagcataac tgtcaaccag actaacaaga gagactatgt ttctaagtat 4921 atcaattaca ttttcaacga ctctgtaaag gcggtttatg aagaatttcg gagaggattt 4981 tataaaatgt gcgacgaaga cattatcaaa ttattccacc ccgaagaact gaaggatgtg 5041 attgttggaa atacagatta tgattggaaa acatttgaaa agaatgcacg ttatgaacca 5101 ggatataaca gttcacatcc caccatagtg atgttttgga aggctttcca caaattgact 5161 ctggaagaaa agaaaaaatt ccttgtattt cttacaggaa ctgacagact acaaatgaaa 5221 gatttaaata atatgaaaat aacattttgc tgtcctgaaa gttggaatga aagagaccct 5281 ataagagcac tgacatgttt cagtgtcctc ttcctcccta aatattctac aatggaaaca 5341 gttgaagaag cgcttcaaga agccatcaac aacaacagag gatttggcgg atccGACTAC 5401 AAAGACCATG ACGGTGATTA TAAAGATCAT GACATCGACT ACAAGGATGA CGATGACAAG 5461 tagcggccgc Gaaggatctg cgatcgctcc ggtgcccgtc agtgggcaga gcgcacatcg 5521 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaacgggtgc ctagagaagg 5581 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt 5641 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt 5701 gccgccagaa cacagctgaa gcttcgaggg gctcgcatct ctccttcacg cgcccgccgc 5761 cctacctgag gccgccatcc acgccggttg agtcgcgttc tgccgcctcc cgcctgtggt 5821 gcctcctgaa ctgcgtccgc cgtctaggta agtttaaagc tcaggtcgag accgggcctt 5881 tgtccggcgc tcccttggag cctacctaga ctcagccggc tctccacgct ttgcctgacc 5941 ctgcttgctc aactctacgt ctttgtttcg ttttctgttc tgcgccgtta cagatccaag 6001 ctgtgaccgg cgcctacgct agacgccacc atggagagcg acgagagcgg cctgcccgcc 6061 atggagatcg agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc 6121 ggcggagagg gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc 6181 gccctgacct tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc 6241 ggcacctacc ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac 6301 accaacaccc gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac 6361 cgctacgagg ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag 6421 gacagcgtga tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac 6481 cccatgggcg ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc 6541 ggctactaca gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc 6601 atcctgcaga acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac 6661 accgagctgg gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga 6721 tcccgcgctc agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga 6781 tctcgcgagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct 6841 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta 6901 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac 6961 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac 7021 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag 7081 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt 7141 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag 7201 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc 7261 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg 7321 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc 7381 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga 7441 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct 7501 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt 7561 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg 7621 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact 7681 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct 7741 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg 7801 ttgggcactg acaattccgt ggtgttgtcg gggaaATCAT CGTCCTTTCC Ttggctgctc 7861 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc 7921 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctT 7981 cgccttcgcc ctcagacgag tcggatctcc ctttggGccg cctccccgcc tggtaccttt 8041 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg 8101 actggaaggg ctaattcact cccaacgaaG ataagatctg ctttttgctt gtactgggtc 8161 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 8221 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 8281 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag 8341 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag 8401 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 8461 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 8521 tgtatcttat catgtctggc tctagctaTC CCGCCCCTAA CTCCGCCCAt cccgccccta 8581 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca 8641 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga 8701 ggcctagact tttgcagaga ccaaattcgt aatcatgtca tagctgtttc ctgtgtgaaa 8761 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg 8821 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca 8881 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg 8941 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 9001 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 9061 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 9121 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 9181 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 9241 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 9301 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 9361 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 9421 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 9481 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 9541 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 9601 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 9661 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 9721 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 9781 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 9841 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 9901 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 9961 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 10021 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 10081 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 10141 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 10201 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 10261 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 10321 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 10381 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 10441 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 10501 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat 10561 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 10621 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 10681 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 10741 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 10801 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 10861 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 10921 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg 10981 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 11041 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct 11101 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc 11161 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa 11221 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 11281 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 11341 aacgacggcc agtgccaagc tg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.