pCMV-PHF20-EGFP-Neo plasmid

pCMV-PHF20-EGFP-Neo plasmid

PVT95061

pCMV-PHF20-EGFP-Neo plasmid Information

Plasmid name: pCMV-PHF20(human)-EGFP-Neo
Alias: NM_016436.5;C20orf104; GLEA2; HCA58; NZF; TDRD20A; TZP
Gene lenght: 3036bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-PHF20-EGFP-Neo plasmid Description

pCMV-PHF20-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-PHF20-EGFP-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atctcgagct caagcttcga attcGCCACC atgacaaagc atccacctaa 661 cagacgagga atcagctttg aagtgggagc ccagttggaa gcccgggacc gtttaaaaaa 721 ctggtatcca gctcacatag aagacattga ctacgaggaa ggaaaagtac tcatccattt 781 caagcgttgg aaccatcgtt atgatgagtg gttctgctgg gacagtcctt atttacgccc 841 tttagagaaa atacagctga ggaaagaggg cttgcatgaa gaggatggat cttctgaatt 901 tcaaataaat gagcaggtcc ttgcttgctg gtctgattgt cgtttttacc cggccaaagt 961 cactgctgtt aacaaggatg gtacttacac tgtgaaattt tatgatggag tagttcagac 1021 tgtcaaacat attcatgtca aagctttttc caaagatcag aatattgtgg gtaatgctag 1081 gcctaaagaa acagatcaca aaagtctttc atcatctcct gataaacgag agaagtttaa 1141 agaacagaga aaagcaacag tgaatgtgaa gaaagacaaa gaagataaac ccttaaagac 1201 agaaaagcga cccaagcagc ctgataaaga aggaaagtta atctgttctg aaaaggggaa 1261 agtgtcagag aaaagtcttc ccaagaacga gaaggaagac aaggaaaaca tttccgaaaa 1321 tgacagagag tattctggag atgcccaagt ggataagaaa cctgaaaatg acattgtgaa 1381 gagtccacaa gaaaacttga gggaacccaa aagaaaacga ggcagacccc cttccatagc 1441 tcctactgct gtggattcaa actctcaaac tttgcaacca ataacattgg aactgagaag 1501 aaggaaaata tcaaaaggat gtgaagtccc attaaaacgt cctcggcttg acaaaaattc 1561 atcccaggaa aagtcaaaaa actactcgga aaacactgac aaagacttat cgaggagacg 1621 ttcctccagg ctgtccacta atgggaccca tgagatccta gatcctgact tggttgtatc 1681 agatttggtt gatacggatc ctttgcaaga cacgttgtct agtaccaagg aatctgaaga 1741 aggtcagttg aagtctgctt tggaagctgg ccaggtctca tctgcactga cttgccactc 1801 ctttggggat ggatccgggg ctgcaggctt ggagttgaac tgcccatcaa tgggagaaaa 1861 cacgatgaaa acagaaccga cttctcccct tgtggaatta caagagattt cgactgtgga 1921 agtaacaaat acttttaaga aaacagatga ttttgggtca tctaatgcac cagctgtcga 1981 cctagaccat aagtttagat gcaaagttgt ggactgttta aaatttttcc gcaaagccaa 2041 actgttgcac tatcacatga agtatttcca tggaatggag aagtcactgg agccagaaga 2101 gagcccggga aagaggcatg tccaaaccag gggcccttca gcttcagaca agcccagcca 2161 ggagaccctg accaggaagc gggtctctgc cagttcccca actacaaaag acaaggaaaa 2221 gaataaagag aagaaattca aggagtttgt gagagtgaag ccaaagaaga aaaagaaaaa 2281 gaaaaagaaa accaaacctg aatgcccctg cagtgaggag atcagtgaca cctcccagga 2341 accttctcca cccaaggcat ttgctgttac caggtgtggg tcctcacaca agccaggggt 2401 ccatatgagc ccgcagcttc atggcccaga atctggacac cacaaaggga aagtgaaagc 2461 attggaggag gataatttga gtgagtcctc ttctgagagc tttctctgga gtgatgatga 2521 gtatggccaa gatgtggatg tgaccaccaa cccagatgag gaacttgatg gggatgaccg 2581 ctatgacttc gaggtggtcc gctgcatctg tgaggtccag gaggaaaatg acttcatgat 2641 tcagtgtgaa gagtgccagt gctggcagca tggggtctgc atgggattac tggaagaaaa 2701 tgtgcccgag aaatacacct gttatgtttg ccaagaccct ccaggtcaga ggcctggctt 2761 caagtactgg tatgacaagg agtggctgag caggggacat atgcatggcc tggcatttct 2821 agaagagaac tactcccatc agaatgccaa gaagatcgtg gccacccacc agcttcttgg 2881 tgatgtgcag agagtgattg aggttctgca tggcctgcag ctcaagatga gcatcttgca 2941 aagccgggag catcctgatc tgccgctgtg gtgccagcct tggaaacagc actcagggga 3001 ggggagatct catttcagaa acatccctgt cactgacacc aggagcaagg aggaagctcc 3061 aagctataga actttgaacg gggcagtgga gaagcccagg cccctggccc tgcccctgcc 3121 gcgttctgtg gaggaatcct atatcaccag tgagcattgc taccagaagc cccgcgccta 3181 ttaccctgcc gtggagcaga agctggtggt ggagacgagg ggctctgccc tcgacgatgc 3241 ggtcaacccc ctccatgaga acggcgatga ttccctttcc ccgcgcctgg gctggcctct 3301 agaccaagac aggagcaagg gggacagtga ccccaaaccc ggctccccaa aggtgaagga 3361 atatgtctcc aaaaaggccc taccagaaga agcccctgct cggaagctgc tggacagagg 3421 tggagagggg ctgctgagct cccagcacca gtggcagttt aacctgctga cccatgtgga 3481 atctcttcag gatgaagtta cgcacaggat ggactccatt gagaaggagt tggatgtgtt 3541 ggagagctgg ctggactaca ctggggaact ggagccccct gagccgctgg ccaggcttcc 3601 gcagctcaag cattgtatca agcagctgct gatggacctg ggcaaggtgc agcagatcgc 3661 cctctgctgc tcaacaggtg gaggcggatc aggaggcggt ggatctgcgg atccaccggt 3721 cgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 3781 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 3841 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 3901 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca 3961 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 4021 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 4081 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 4141 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca 4201 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca 4261 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga 4321 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca 4381 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta 4441 caagtaaagc ggccgcgact ctagatcata atcagccata ccacatttgt agaggtttta 4501 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt 4561 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 4621 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 4681 aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt 4741 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc 4801 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt 4861 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact 4921 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg 4981 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag 5041 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac 5101 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg 5161 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa 5221 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa 5281 gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc 5341 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg 5401 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag 5461 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc 5521 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 5581 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc 5641 aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca 5701 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 5761 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 5821 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc 5881 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 5941 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 6001 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 6061 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 6121 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 6181 cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca 6241 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 6301 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 6361 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 6421 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact 6481 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc 6541 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg 6601 atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact 6661 gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag 6721 aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg 6781 cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt 6841 tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg 6901 gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat 6961 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct 7021 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct 7081 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca 7141 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc 7201 agcagagcgc agataccaaa tactgtTctt ctagtgtagc cgtagttagg ccaccacttc 7261 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct 7321 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag 7381 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc 7441 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg 7501 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag 7561 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt 7621 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac 7681 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg 7741 ttatcccctg attctgtgga taaccgtatt accgccatgc at

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.