pT3-EF1a-Trnp1 plasmid

pT3-EF1a-Trnp1 plasmid

PVT94894

pT3-EF1a-Trnp1 plasmid Information

Plasmid name: pT3-EF1a-Trnp1(mouse)
Alias: NM_001081156.2;2300002D11Rik; Trnp
Gene lenght: 672bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: EF1a
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: T7
Reverse primer:
Copy number: High
Induction:

pT3-EF1a-Trnp1 plasmid Description

pT3-EF1a-Trnp1 is a plasmid suitable for protein expression in mammalian cells.

pT3-EF1a-Trnp1 plasmid Sequence

1 GGCTCCGGTG CCCGTCAGTG GGCAGAGCGC ACATCGCCCA CAGTCCCCGA GAAGTTGGGG 61 GGAGGGGTCG GCAATTGAAC CGGTGCCTAG AGAAGGTGGC GCGGGGTAAA CTGGGAAAGT 121 GATGTCGTGT ACTGGCTCCG CCTTTTTCCC GAGGGTGGGG GAGAACCGTA TATAAGTGCA 181 GTAGTCGCCG TGAACGTTCT TTTTCGCAAC GGGTTTGCCG CCAGAACACA GGTAAGTGCC 241 GTGTGTGGTT CCCGCGGGCC TGGCCTCTTT ACGGGTTATG GCCCTTGCGT GCCTTGAATT 301 ACTTCCACCT GGCTGCAGTA CGTGATTCTT GATCCCGAGC TTCGGGTTGG AAGTGGGTGG 361 GAGAGTTCGA GGCCTTGCGC TTAAGGAGCC CCTTCGCCTC GTGCTTGAGT TGAGGCCTGG 421 CCTGGGCGCT GGGGCCGCCG CGTGCGAATC TGGTGGCACC TTCGCGCCTG TCTCGCTGCT 481 TTCGATAAGT CTCTAGCCAT TTAAAATTTT TGATGACCTG CTGCGACGCT TTTTTTCTGG 541 CAAGATAGTC TTGTAAATGC GGGCCAAGAT CTGCACACTG GTATTTCGGT TTTTGGGGCC 601 GCGGGCGGCG ACGGGGCCCG TGCGTCCCAG CGCACATGTT CGGCGAGGCG GGGCCTGCGA 661 GCGCGGCCAC CGAGAATCGG ACGGGGGTAG TCTCAAGCTG GCCGGCCTGC TCTGGTGCCT 721 GGCCTCGCGC CGCCGTGTAT CGCCCCGCCC TGGGCGGCAA GGCTGGCCCG GTCGGCACCA 781 GTTGCGTGAG CGGAAAGATG GCCGCTTCCC GGCCCTGCTG CAGGGAGCTC AAAATGGAGG 841 ACGCGGCGCT CGGGAGAGCG GGCGGGTGAG TCACCCACAC AAAGGAAAAG GGCCTTTCCG 901 TCCTCAGCCG TCGCTTCATG TGACTCCACG GAGTACCGGG CGCCGTCCAG GCACCTCGAT 961 TAGTTCTCGA GCTTTTGGAG TACGTCGTCT TTAGGTTGGG GGGAGGGGTT TTATGCGATG 1021 GAGTTTCCCC ACACTGAGTG GGTGGAGACT GAAGTTAGGC CAGCTTGGCA CTTGATGTAA 1081 TTCTCCTTGG AATTTGCCCT TTTTGAGTTT GGATCTTGGT TCATTCTCAA GCCTCAGACA 1141 GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GAATTAGCTT GGTACTAATA 1201 CGACTCACTA TAGGGAGACC CAAGCTGGCT AGGTAAGCTT GGTACCGAGC TCGGATCCAC 1261 TAGTCCAGTG TGGTGGAATT CTGCAGATAT CAACAAGTTT GTACAAAAAA GCAGGCTatg 1321 ccgggctgcc gaatcagcgc ctgcggccca ggggcccagg aagggacggc agaaccgggg 1381 tcgcccccgc cgccaccccg ggagcccctg ccgtccctcc agcccccttc cccatcgccg 1441 acctcgactc cgacccccac taagtcaccg cccttacccg aggcggccga gacacccgtg 1501 gaagggcagg agctgcagcg ctggcgccag ggtgctagtg gggggtctgg gggcgcgggc 1561 ccggcgggga tcgcgggcgc ggcggcgggg gcagggggcc gtgcgctgga gctggccgaa 1621 gcgcggcggc gactgctaga agtggagggc cgccggcgcc tggtgtcgga gctggagagc 1681 cgggtgctgc agctgcaccg agtcttcttg gctgcggagc tgcgcctggc gcatcgcgcc 1741 gagagcctga gccgcctgag cggcggcgtg gcccaggccg agctctacct ggctgcgcac 1801 gggtcccgtc tcaagaaggg tgcgcgccgc ggtcgccggg gtcgtccccc ggcgctgctg 1861 gcgtccgcgc tcggcctggg gagctgcgtg ccctggggcg cagggcggct acggcgcggc 1921 cagggcccgg agcccgactc gcccttccgc cgcagcccgc cccgcggccc cgcctccccc 1981 cagcgctgaC CATGGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC 2041 CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG 2101 TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC 2161 TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG 2221 CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG 2281 GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAATAACTT CGTATAATGT ATGCTATACG 2341 AAGTTATATC GTATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 2401 ACACCCTAAC TGACACACAT TCCACAGAAT TCCTTGAAAT ACATCCACAG GTACACCTCC 2461 AATTGACTCA AATGATGTCA ATTAGTCTAT CAGAAGCTTC TAAAGCCATG ACATCATTTT 2521 CTGGAATTTT CCAAGCTGTT TAAAGGCACA GTCAACTTAG TGTATGTAAA CTTCTGACCC 2581 ACTGGAATTG TGATACAGTG AATTATAAGT GAAATAATCT GTCTGTAAAC AATTGTTGGA 2641 AAAATGACTT GTGTCATGCA CAAAGTAGAT GTCCTAACTG ACTTGCCAAA ACTATTGTTT 2701 GTTAACAAGA AATTTGTGGA GTAGTTGAAA AACGAGTTTT AATGACTCCA ACTTAAGTGT 2761 ATGTAAACTT CCGACTTCAA CTGTAGGGTA CCGAGCTCGA ATTCACTGGC CGTCGTTTTA 2821 CAACGTCGTG ACTGGGAAAA CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC 2881 CCTTTCGCCA GCTGGCGTAA TAGCGAAGAG GCCCGCACCG ATCGCCCTTC CCAACAGTTG 2941 CGCAGCCTGA ATGGCGAATG GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT 3001 ATTTCACACC GCATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC 3061 CAGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA 3121 TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG 3181 TCATCACCGA AACGCGCGAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT 3241 GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA 3301 ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA 3361 CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT 3421 GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG 3481 CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG 3541 GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG 3601 AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTATTGACGC CGGGCAAGAG 3661 CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA 3721 GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG 3781 AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC 3841 GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG 3901 AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGTAGCAAT GGCAACAACG 3961 TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC 4021 TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG 4081 TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG 4141 GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT 4201 ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA 4261 CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT 4321 AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG 4381 TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT 4441 TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT 4501 TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG 4561 CAGATACCAA ATACTGTTCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT 4621 GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC 4681 GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG 4741 TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA 4801 CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG 4861 GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG 4921 GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA 4981 TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT 5041 TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT 5101 GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA 5161 ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT ACGCAAACCG 5221 CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT TCCCGACTGG 5281 AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG 5341 GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT 5401 CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTTG CATGCCTGCA GGTCGACTCT 5461 AGACCTATAC AGTTGAAGTC GGAAGTTTAC ATACACTTAA GTTGGAGTCA TTAAAACTCG 5521 TTTTTCAACT ACTCCACAAA TTTCTTGTTA ACAAACAATA GTTTTGGCAA GTCAGTTAGG 5581 ACATCTACTT TGTGCATGAC ACAAGTCATT TTTCCAACAA TTGTTTACAG ACAGATTATT 5641 TCACTTATAA TTCACTGTAT CACAATTCCA GTGGGTCAGA AGTTTACATA CACTAAGTTG 5701 ACTGTGCCTT TAAACAGCTT GGAAAATTCC AGAAAATGAT GTCATGGCTT TAGAAGCTTC 5761 TGATAGACTA ATTGACATCA TTTGAGTCAA TTGGAGGTGT ACCTGTGGAT GTATTTCAAG 5821 GGGATCCAGA CATGATAAGA TACATTGATG AGTTTGGACA AACCACAACT AGAATGCAGC 5881 GGCCGCTAGC TCGAGATCTT AATTAAACTA GTATCGATAT AACTTCGTAT AATGTATGCT 5941 ATACGAAGTT ATATGTACGG GCCAGATATA CGCGTTGACA TTGATTATTG ACTAGGCTTT 6001 TGCAAAAAGC TTTGCAAAGA TGGATAAAGT TTTAAACAGA GAGGAATCTT TGCAGCTAAT 6061 GGACCTTCTA GGTCTTGAAA GGAGTGGGAA TT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.