pCMV-FAM83H-3×FLAG-mCMV-EGFP p

pCMV-FAM83H-3×FLAG-mCMV-EGFP plasmid

PVT94887

pCMV-FAM83H-3×FLAG-mCMV-EGFP plasmid Information

Plasmid name: pCMV-FAM83H(human)-3×FLAG-mCMV-EGFP
Alias: NM_198488.5;AI3; AI3A;
Gene lenght: 3537bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-FAM83H-3×FLAG-mCMV-EGFP plasmid Description

pCMV-FAM83H-3×FLAG-mCMV-EGFP is a plasmid suitable for protein expression in mammalian cells.

pCMV-FAM83H-3×FLAG-mCMV-EGFP plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc GCTAGCGCTA 601 CCGGACTCAG ATCTCGAGCT CCGAATTCGC CACCatggcc cgtcgctctc agagctcctc 661 gcagggggac aacccactgg cacccgggta cctgccgcct cactacaaag agtactaccg 721 cctggcggtg gatgcactgg ccgagggtgg ctcggaggcc tacagccgct tcctcgctac 781 cgagggggca ccagacttcc tgtgccctga agagctggaa catgtgagcc gacaccttcg 841 gcctccgcag tatgttaccc gagagccacc tgaaggcagc cttctcgacg tggacatgga 901 tggctcctcg ggtacatact ggccagtgaa ctcagaccag gccgtgcctg agcttgattt 961 gggctggcct ctgaccttcg gcttccaggg caccgaggtg accaccttgg tgcagccacc 1021 gccccccgac agccccagta tcaaggatga ggcccgcagg atgatccgtt ccgcccagca 1081 ggtggtggcc gtggtgatgg acatgttcac tgatgtggac ctgctcagcg aagtgctgga 1141 ggccgcggcc cgtcgggtcc cagtctacat cctgctggat gagatgaacg cgcagcactt 1201 cctggacatg gccgacaagt gccgtgtcaa cctgcagcac gtggatttcc tgcgcgtacg 1261 gactgtggcg ggccccacct actactgccg cactgggaag tccttcaagg gccacgtcaa 1321 ggagaagttc ctgctggtgg actgtgccgt ggtgatgagt gggagctaca gcttcatgtg 1381 gtcctttgag aagatccacc gcagcctggc gcacgtgttc caaggagagc tggtctccag 1441 cttcgacgag gagttccgca tcctcttcgc gcagtccgag ccgcttgtgc cctcggccgc 1501 ggccctggcc cgcatggacg cctatgccct ggctccgtat gccggggccg ggcctctcgt 1561 gggcgtccct ggggtcgggg cgccaacccc cttctccttc cctaaacgag cgcacctcct 1621 gttcccgcca ccccgggaag agggcctggg cttcccctcc ttcctcgacc cggaccgcca 1681 cttcctgtcg gccttccgcc gggaggagcc gccgcggatg ccggggggcg cgctggaacc 1741 gcacgcgggg ctgcggccgc tctcgcggcg cctggaggcc gaggccgggc cggctgggga 1801 gctcgcgggc gcgcggggct tcttccaggc gcggcacctg gagatggacg ccttcaagcg 1861 gcacagcttc gcgaccgagg gcgcgggcgc cgtggagaac ttcgcggccg cgcggcaggt 1921 gtcgcggcag acgttcctca gccacggcga cgacttccgc ttccagacca gccacttcca 1981 ccgtgaccag ctctaccagc agcagtacca gtgggacccg cagctcacgc cggcgcgccc 2041 gcaaggcctg ttcgagaagc ttcgcggggg ccgcgcgggt ttcgcggacc cggatgactt 2101 caccctgggc gccgggcccc gcttcccgga gctcggaccc gacgggcacc agcggctgga 2161 ctacgtgccg tccagcgcgt cccgcgaggt gcgccacggc tcggaccccg ccttcgcgcc 2221 cggaccccgc ggcctggagc ccagcggagc cccgcgcccc aacctgaccc agcgcttccc 2281 atgccaggcc gcggcgaggc cgggcccaga ccccgctccc gaggcggagc cggagcgcag 2341 gggcgggccc gaggggcggg cagggctgcg gcgctggcgt ttggcctcct acttgagcgg 2401 ctgccacggc gaggatgggg gcgacgacgg cctaccggcg cccatggaag cggaggctta 2461 cgaagacgac gtgctggctc ccgggggccg ggcacctgcc ggcgacctgc tcccctcggc 2521 cttccgcgtc ccagcagcct tccccaccaa ggtcccggtg ccaggcccgg gcagcggcgg 2581 caacggccca gagcgcgagg gcccggagga gcctggcctg gccaagcagg actcattccg 2641 ctcgcgcctg aaccccctgg tccagcgcag ctccaggctg cgctcctcgc tcatcttcag 2701 cacgtcacag gccgagggcg cggccggggc tgcggcggcc actgagaagg tgcagctgct 2761 gcacaaggag cagacggtca gcgagacgct ggggcccggc ggagaggccg tgcgctccgc 2821 ggcttccacc aaggtggcgg agctgctgga gaagtacaag ggcccagccc gtgatcccgg 2881 cggcggcgcg ggcgccatca ccgttgccag ccacagcaag gccgtcgtgt cccaggcgtg 2941 gcgggaagag gtggcggccc caggtgccgt ggggggcgag cgccgcagcc tcgagagctg 3001 cctgctggac ctgcgcgact cctttgcaca gcagctgcac caggaggcgg agcggcagcc 3061 gggagccgcg tcgctcaccg cggcgcagct gctcgacaca ctgggccgga gcggctccga 3121 ccgcctgcct tcccgcttcc tctctgccca gagccactca acgtccccgc aagggctgga 3181 cagccctctg ccgctggaag ggtccggagc gcaccaggtg ctccataatg agtcaaaagg 3241 gagccccacc tcggcttacc ctgagcggaa ggggagcccc acgcctgggt tttccactcg 3301 aagaggaagt ccaactacag gatttatcga gcagaagggg agccccacct cagcctaccc 3361 cgagcgcagg ggtagtccgg tgccccccgt gccggagcgc aggagcagtc cggtgccccc 3421 cgtgccggag cgcaggggca gcctcaccct taccatctcc ggggagtccc cgaaggccgg 3481 gcccgcggag gaggggccga gcggccccat ggaagtcctg cgcaaaggct ccttgcgtct 3541 taggcagctg ctgagcccca agggcgagcg gcgcatggag gatgagggtg gcttcccagt 3601 gccgcaggag aacggccaac ccgagagccc gcggcgtctg tcactgggcc agggtgacag 3661 cacggaggct gccacagaag agcggggtcc gcgggcgcgc ctgtcctcag ccacggccaa 3721 cgccttgtac agcagcaacc ttcgggatga cacgaaggcc attctggagc agatcagtgc 3781 ccacggccag aagcaccgtg cggtccctgc cccgagcccc ggcccgaccc acaacagccc 3841 cgagctaggc cgtccaccgg ctgctggcgt cctggcccca gatatgtccg acaaggacaa 3901 gtgttcagcc atcttccgct cggacagctt ggggacccag ggccggctga gccgcacgct 3961 gccagccagc gcggaggagc gcgatcggct gctgcgccgc atggagagca tgcgcaagga 4021 gaagcgcgtg tacagccgct tcgaggtctt ctgcaagaaa gaggaggcca gcagccctgg 4081 ggcaggggaa ggccccgcgg aggagggcac cagggacagc aaggtgggca agttcgtgcc 4141 caagatcctg ggcacgttca aaagcaagaa gAAGCTTACG CGTGACTACA AAGACCATGA 4201 CGGTGATTAT AAAGATCATG ACATCGACTA CAAGGATGAC GATGACAAGT GAGGATCCGG 4261 GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT 4321 TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA 4381 ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG 4441 GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG CTGGGGATGC 4501 GGTGGGCTCT ATGGTCTAGA GATATACTGA GTCATTAGGG ACTTTCCAAT GGGTTTTGCC 4561 CAGTACATAA GGTCAATAGG GGTGAATCAA CAGGAAAGTC CCATTGGAGC CAAGTACACT 4621 GAGTCAATAG GGACTTTCCA TTGGGTTTTG CCCAGTACAA AAGGTCAATA GGGGGTGAGT 4681 CAATGGGTTT TTCCCATTAT TGGCACGTAC ATAAGGTCAA TAGGGGTGAG TCATTGGGTT 4741 TTTCCAGCCA aTTTAATTAA AACGCCATGT ACTTTCCCAC CATTGACGTC AATGGGCTAT 4801 TGAAACTAAT GCAACGTGAC CTTTAAACGG TACTTTCCCA TAGCTGATTA ATGGGAAAGT 4861 ACCGTTCTCG AGCCAATACA CGTCAATGGG AAGTGAAAGG GCAGCCAAAA CGTAACACCG 4921 CCCCGGTTTT CCCCTGGAAA TTCCATATTG GCACGCATTC TATTGGCTGA GCTGCGTTCT 4981 ACGTGGGTAT AAGAGGCGCG ACCAGCGTCG GTACCGTCGC AGTCTTCGGT CTGACCACCG 5041 TAGAACGCAG ATCaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg 5101 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg 5161 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg 5221 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct 5281 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag 5341 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg 5401 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca 5461 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct 5521 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca 5581 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg 5641 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc 5701 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc 5761 tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac 5821 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa 5881 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa 5941 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg 6001 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt 6061 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg 6121 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt 6181 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct 6241 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt 6301 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa 6361 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc 6421 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc 6481 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat 6541 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc 6601 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca 6661 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 6721 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca 6781 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc 6841 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta 6901 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt 6961 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga 7021 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct 7081 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc 7141 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag 7201 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg 7261 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc 7321 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc 7381 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg 7441 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc 7501 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg 7561 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc 7621 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag 7681 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg 7741 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg 7801 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc 7861 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt 7921 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca 7981 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat 8041 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg 8101 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata 8161 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct 8221 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta 8281 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 8341 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 8401 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 8461 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 8521 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc 8581 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 8641 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 8701 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 8761 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 8821 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 8881 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 8941 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 9001 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc 9061 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca 9121 t

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.