pLV3-CMV-Pdgfrb-3×FLAG-CopGFP-

pLV3-CMV-Pdgfrb-3×FLAG-CopGFP-Puro plasmid

PVT94852

pLV3-CMV-Pdgfrb-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Pdgfrb(mouse)-3×FLAG-CopGFP-Puro
Alias: NM_001146268.1;CD140b; Pdgfr; PDGFR-1
Gene lenght: 3297bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Pdgfrb-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Pdgfrb-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Pdgfrb-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg ggcttccagg agtgatacca 2341 gctttagtcc tcagaggcca gttgttgctg tccgtgttat ggctcctggg accgcagacc 2401 tcccggggcc tagtcatcac gccccctggg ccagagtttg ttctcaacat ctccagcacc 2461 tttgttctga cctgctcggg ctcagctccg gtgatgtggg aacagatgtc ccaggtgccc 2521 tggcaagaag cggccatgaa tcaggacggc accttctcca gtgtgctgac actgaccaat 2581 gtcactgggg gagacactgg ggaatacttt tgtgtctaca acaactcact agggccggag 2641 ctcagtgaga ggaagcgtat ctatatcttt gtgccagatc ccaccatggg cttcctccct 2701 atggactccg aggacctgtt catttttgtc acggatgtca ctgagacgac aattccgtgc 2761 cgagtgacag acccccagct ggaggtgacg ctacatgaga agaaagtgga tatcccccta 2821 cacgtaccct acgaccacca gcgaggtttc actggtactt ttgaggacaa gacttacatt 2881 tgcaaaacca ccattgggga cagggaagtg gactccgata cttactacgt ctacagcctc 2941 caggtgtcat ccatcaacgt ctctgtgaat gccgtgcaga ctgtggtccg ccagggcgag 3001 agcatcacca tccggtgcat tgtgatgggc aatgatgtgg tgaacttcca atggacgtac 3061 ccccgcatga agagtgggcg gctggtggag ccagtgacag actacctctt tggagtgccc 3121 tcccgcattg gctccatcct gcatatcccc acggctgagc tgagtgattc gggcacctat 3181 acttgcaacg tgtcagtgag tgtgaacgac catggcgatg agaaagccat caacatctct 3241 gtgatcgaga atggctacgt gcggctgctg gagacactgg gagatgtaga aattgctgag 3301 ctgcaccgga gtcggacgct gcgggtggtg ttcgaggctt atccgatgcc ttctgtcctg 3361 tggctcaagg acaaccgtac cttgggtgac tccggcgctg gcgagttagt tttgtctact 3421 cgcaacatgt ctgagacccg gtacgtgtca gaactgatcc tggtacgtgt gaaggtgtca 3481 gaagcgggct actatactat gcgagccttc cacgaggacg atgaggtcca gctctccttc 3541 aagctgcagg tcaatgtccc cgtccgtgtg ctggagctga gtgagagtca ccctgccaat 3601 ggggagcaga caatccgctg tcgtggccgg ggcatgcctc agccaaatgt cacctggtct 3661 acctgcagag acctcaaaag taggtgtcca cgaaaactgt cacccacacc cttggggaat 3721 agttccaagg aggagagcca gctagaaacg aatgtgactt tctgggagga agatcaggaa 3781 tacgaggtgg tgagcacact gcgcctgcgc cacgtggatc agccactgtc cgtacgctgc 3841 atgctgcaga actccatggg tggagattcg caggaggtca ccgtggtccc acattccttg 3901 cccttcaaag tggtggtgat ctcagccatc ctggccttag tggtccttac cgtcatctct 3961 ctcatcatcc tcatcatgct gtggcagaag aagccacgct atgagatccg atggaaggtc 4021 attgagtctg tgagctctga cggtcatgag tacatctacg tggaccctgt gcagttgcct 4081 tacgactcca cctgggagct gccacgggac cagcttgttc tgggacgcac tcttggctct 4141 ggggctttcg gacaggtggt ggaggccaca gctcacggtc tgagccattc gcaggccacc 4201 atgaaagtgg ctgtcaagat gctgaaatcg acagccagaa gtagcgagaa gcaagcctta 4261 atgtccgagc tgaagattat gagtcatctt ggaccccacc tgaacgtggt caacctgctg 4321 ggggcctgca ccaaaggagg gcccatctac atcatcacgg aatactgccg atacggtgat 4381 ctggtggact acctgcaccg gaacaaacac accttcttgc agcgacactc caacaagcat 4441 tgtccgccca gtgctgagct ctacagcaac gccctgccag tggggttctc cctacccagc 4501 cacttgaacc tgactgggga gagtgacggt ggctacatgg atatgagcaa ggatgaatct 4561 atagattacg tgcccatgtt ggacatgaaa ggagacatca aatacgcaga cattgagtcc 4621 cccagctaca tggcccctta tgataactat gtcccatctg cccctgaaag gacctatcgc 4681 gccaccttaa tcaacgactc accagtgctc agctacacag acctcgtggg cttcagctac 4741 caagtggcca acggcatgga cttcttagcc tctaagaact gtgttcaccg agacttggcg 4801 gccaggaatg tgctcatctg cgagggcaag ctggtcaaga tctgtgactt cggcctggct 4861 cgagacatca tgagggactc aaactacatc tccaaaggca gcaccttcct gcctctgaag 4921 tggatggccc cagagagcat cttcaacagc ctctacacca ctttgagtga tgtctggtct 4981 tttgggatcc tactctggga gatcttcaca ctgggtggca ccccttaccc agagctgccc 5041 atgaacgacc agttctacaa tgccatcaag aggggctacc gcatggccca gcctgctcat 5101 gcctccgacg agatctatga gatcatgcag aaatgctggg aagaaaagtt tgagactcga 5161 ccccccttct cccagctggt gctgctcctg gagaggcttc tgggtgaagg ctataaaaag 5221 aagtaccagc aggtagatga ggagttcctg aggagtgacc atcctgccat cctgaggtcc 5281 caagcccgct ttccggggat ccacagcctc cgatcccctc tggacaccag ctctgttctc 5341 tacactgccg tgcagcccaa tgagagtgac aatgactaca tcatcccctt acctgacccc 5401 aagcctgacg ttgctgatga aggtctccca gaggggtccc ccagccttgc cagttccacc 5461 ttgaatgaag tcaacacttc ctccaccatc tcctgcgaca gtcccctgga gctccaagaa 5521 gagccacagc aagcagagcc tgaggcacaa ctggagcagc cacaggattc aggctgccca 5581 ggacctctgg ctgaagcaga ggacagcttc ctgggatccG ACTACAAAGA CCATGACGGT 5641 GATTATAAAG ATCATGACAT CGACTACAAG GATGACGATG ACAAGtagcg gccgcGaagg 5701 atctgcgatc gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag 5761 aagttggggg gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac 5821 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat 5881 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag 5941 ctgaagcttc gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc 6001 catccacgcc ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg 6061 tccgccgtct aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct 6121 tggagcctac ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc 6181 tacgtctttg tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct 6241 acgctagacg ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc 6301 cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc 6361 cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc 6421 ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc 6481 ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc 6541 gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc 6601 cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc 6661 accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac 6721 gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc 6781 gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg 6841 ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc 6901 gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg 6961 tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga 7021 ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag 7081 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc 7141 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg 7201 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg 7261 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg 7321 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg 7381 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg 7441 gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc 7501 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc 7561 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc 7621 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat 7681 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt 7741 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc 7801 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg 7861 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc 7921 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa 7981 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat 8041 tccgtggtgt tgtcggggaa ATCATCGTCC TTTCCTtggc tgctcgcctg tgttgccacc 8101 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt 8161 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtctTcgcct tcgccctcag 8221 acgagtcgga tctccctttg gGccgcctcc ccgcctggta cctttaagac caatgactta 8281 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat 8341 tcactcccaa cgaaGataag atctgctttt tgcttgtact gggtctctct ggttagacca 8401 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag 8461 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag 8521 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct 8581 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt 8641 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc 8701 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt 8761 ctggctctag ctaTCCCGCC CCTAACTCCG CCCAtcccgc ccctaactcc gcccagttcc 8821 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc 8881 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc 8941 agagaccaaa ttcgtaatca tgtcatagct gtttcctgtg tgaaattgtt atccgctcac 9001 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 9061 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 9121 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 9181 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 9241 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 9301 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 9361 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 9421 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 9481 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 9541 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 9601 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 9661 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 9721 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 9781 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 9841 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 9901 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 9961 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 10021 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 10081 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 10141 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 10201 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 10261 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 10321 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 10381 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 10441 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 10501 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 10561 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 10621 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 10681 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 10741 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 10801 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 10861 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 10921 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 10981 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 11041 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 11101 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 11161 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 11221 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 11281 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 11341 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 11401 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 11461 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 11521 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 11581 caagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.