pLV3-CMV-DPYSL2-EF1a-CopGFP-Pu

pLV3-CMV-DPYSL2-EF1a-CopGFP-Puro plasmid

PVT94834

pLV3-CMV-DPYSL2-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-DPYSL2(human)-EF1a-CopGFP-Puro
Alias: NM_001197293.3;CRMP-2; CRMP2; DHPRP2; DRP-2; DRP2; N2A3;ULIP-2; ULIP2
Gene lenght: 2034bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-DPYSL2-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-DPYSL2-EF1a-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-DPYSL2-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatgg ccgagagaaa gcaatccggg 2341 aaggcggcag aggacgaaga ggtccctgct ttttttaaaa acctgggctc cggcagcccc 2401 aagccccggc agaaattctg tggcatgttc tgcccggtgg aagggtcctc ggagaacaag 2461 accatcgact tcgactcgct gtcggtgggc cggggctcgg ggcaggtggt ggctcagcag 2521 cgggacgtcg cccacttggg cccggacccg cagccgccgt actcgcggca gggccggcgc 2581 gccggcggag agccatctgt tgaatcgggc cggaaggtgg agatccggag ggcctcgggc 2641 aaagaagccc tgcagaacat caacgaccag agcgatcgtc ttctgatcaa aggaggtaaa 2701 attgttaatg atgaccagtc gttctatgca gacatataca tggaagatgg gttgatcaag 2761 caaataggag aaaatctgat tgtgccagga ggagtgaaga ccatcgaggc ccactcccgg 2821 atggtgatcc ccggaggaat tgacgtccac actcgtttcc agatgcctga tcagggaatg 2881 acgtctgctg atgatttctt ccaaggaacc aaggcggccc tggctggggg aaccactatg 2941 atcattgacc acgttgttcc tgagcctggg acaagcctgc tcgctgcctt tgaccagtgg 3001 agggaatggg ccgacagcaa gtcctgctgt gactactctc tgcatgtgga catcagtgag 3061 tggcataagg gcatccagga ggagatggaa gcgcttgtga aggatcacgg ggtaaattcc 3121 ttcctcgtgt acatggcttt caaagatcgc ttccagctaa cggattgcca gatttatgaa 3181 gtactgagtg tgatccggga tattggcgcc atagcccaag tccacgcaga aaatggcgac 3241 atcattgcag aggagcagca gaggatcctg gatctgggca tcacgggccc cgagggacat 3301 gtgctgagcc gacctgagga ggtcgaggcc gaagccgtga atcgtgccat caccatcgcc 3361 aaccagacca actgcccgct gtatatcacc aaggtgatga gcaaaagctc tgctgaggtc 3421 atcgcccagg cacggaagaa gggaactgtg gtgtatggcg agcccatcac tgccagcttg 3481 ggaacggacg gctcccatta ctggagcaag aactgggcca aggctgctgc ctttgtcacc 3541 tccccaccct tgagccctga tccaaccact ccagactttc tcaactcctt gctgtcctgt 3601 ggagacctcc aggtcacggg cagtgcccat tgcacgttta acactgccca gaaggctgta 3661 ggaaaggaca acttcaccct gattccggag ggcaccaatg gcactgagga gcggatgtcc 3721 gtcatctggg acaaggctgt ggtcactggg aagatggatg agaaccagtt tgtggctgtg 3781 accagcacca atgcagccaa agtcttcaac ctttaccccc ggaaaggccg cattgctgtg 3841 ggatccgatg ccgacctggt catctgggac cccgacagcg ttaaaaccat ctctgccaag 3901 acacacaaca gctctctcga gtacaacatc tttgaaggca tggagtgccg cggctcccca 3961 ctggtggtca tcagccaggg gaagattgtc ctggaggacg gcaccctgca tgtcaccgaa 4021 ggctctggac gctacattcc ccggaagccc ttccctgatt ttgtttacaa gcgtatcaag 4081 gcaaggagca ggctggctga gctgagaggg gttcctcgtg gcctgtatga cggacctgtg 4141 tgtgaagtgt ctgtgacgcc caagacagtc actccagcct cctcggccaa gacgtctcct 4201 gccaagcagc aggccccacc tgtccggaac ctgcaccagt ctggattcag tttgtctggt 4261 gctcagattg atgacaacat tccccgccgc accacccagc gtatcgtggc gccccccggt 4321 ggccgtgcca acatcaccag cctgggctag ggatccCTGC GATATCCAGC gcggccgcGa 4381 aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc 4441 gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta 4501 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg 4561 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca 4621 cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc 4681 cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact 4741 gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc 4801 ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa 4861 ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg 4921 cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag 4981 tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc 5041 acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc 5101 agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc 5161 agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc 5221 atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc 5281 ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc 5341 ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat 5401 aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc 5461 ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac 5521 gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc 5581 atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag 5641 tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc 5701 agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac 5761 aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc 5821 gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag 5881 cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg 5941 tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg 6001 ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc 6061 gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc 6121 ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg 6181 ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg 6241 ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg 6301 cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg 6361 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta 6421 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt 6481 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc 6541 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt 6601 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg 6661 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac 6721 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc 6781 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac 6841 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtctccg ccttcgccct 6901 cagacgagtc ggatctccct ttggccgcct ccccgcctgg tacctttaag accaatgact 6961 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta 7021 attcactccc aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac 7081 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa 7141 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag 7201 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat 7261 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg 7321 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa 7381 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat 7441 gtctggctct agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 7501 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc 7561 agaagtagtg aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt 7621 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 7681 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 7741 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 7801 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 7861 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 7921 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 7981 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 8041 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 8101 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 8161 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 8221 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 8281 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 8341 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 8401 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 8461 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 8521 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 8581 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 8641 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 8701 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 8761 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 8821 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 8881 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 8941 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 9001 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 9061 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 9121 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 9181 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 9241 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 9301 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 9361 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 9421 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 9481 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 9541 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 9601 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 9661 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 9721 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg 9781 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc 9841 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg 9901 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt 9961 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac 10021 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 10081 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct 10141 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa 10201 cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.