pCMV-CBLB-3×Myc-Neo plasmid

pCMV-CBLB-3×Myc-Neo plasmid

PVT94818

pCMV-CBLB-3×Myc-Neo plasmid Information

Plasmid name: pCMV-CBLB(human)-3×Myc-Neo
Alias: NM_170662.5;Cbl-b; Nbla00127; RNF56
Gene lenght: 2946bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-CBLB-3×Myc-Neo plasmid Description

pCMV-CBLB-3×Myc-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-CBLB-3×Myc-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTGGCTA GCGTTTAAAC TTAAGCTTGG 661 TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGAATTCG CCACCatggc aaactcaatg 721 aatggcagaa accctggtgg tcgaggagga aatccccgaa aaggtcgaat tttgggtatt 781 attgatgcta ttcaggatgc agttggaccc cctaagcaag ctgccgcaga tcgcaggacc 841 gtggagaaga cttggaagct catggacaaa gtggtaagac tgtgccaaaa tcccaaactt 901 cagttgaaaa atagcccacc atatatactt gatattttgc ctgatacata tcagcattta 961 cgacttatat tgagtaaata tgatgacaac cagaaacttg cccaactcag tgagaatgag 1021 tactttaaaa tctacattga tagccttatg aaaaagtcaa aacgggcaat aagactcttt 1081 aaagaaggca aggagagaat gtatgaagaa cagtcacagg acagacgaaa tctcacaaaa 1141 ctgtccctta tcttcagtca catgctggca gaaatcaaag caatctttcc caatggtcaa 1201 ttccagggag ataactttcg tatcacaaaa gcagatgctg ctgaattctg gagaaagttt 1261 tttggagaca aaactatcgt accatggaaa gtattcagac agtgccttca tgaggtccac 1321 cagattagct ctggcctgga agcaatggct ctaaaatcaa caattgattt aacttgcaat 1381 gattacattt cagtttttga atttgatatt tttaccaggc tgtttcagcc ttggggctct 1441 attttgcgga attggaattt cttagctgtg acacatccag gttacatggc atttctcaca 1501 tatgatgaag ttaaagcacg actacagaaa tatagcacca aacccggaag ctatattttc 1561 cggttaagtt gcactcgatt gggacagtgg gccattggct atgtgactgg ggatgggaat 1621 atcttacaga ccatacctca taacaagccc ttatttcaag ccctgattga tggcagcagg 1681 gaaggatttt atctttatcc tgatgggagg agttataatc ctgatttaac tggattatgt 1741 gaacctacac ctcatgacca tataaaagtt acacaggaac aatatgaatt atattgtgaa 1801 atgggctcca cttttcagct ctgtaagatt tgtgcagaga atgacaaaga tgtcaagatt 1861 gagccttgtg ggcatttgat gtgcacctct tgccttacgg catggcagga gtcggatggt 1921 cagggctgcc ctttctgtcg ttgtgaaata aaaggaactg agcccataat cgtggacccc 1981 tttgatccaa gagatgaagg ctccaggtgt tgcagcatca ttgacccctt tggcatgccg 2041 atgctagact tggacgacga tgatgatcgt gaggagtcct tgatgatgaa tcggttggca 2101 aacgtccgaa agtgcactga caggcagaac tcaccagtca catcaccagg atcctctccc 2161 cttgcccaga gaagaaagcc acagcctgac ccactccaga tcccacatct aagcctgcca 2221 cccgtgcctc ctcgcctgga tctaattcag aaaggcatag ttagatctcc ctgtggcagc 2281 ccaacgggtt caccaaagtc ttctccttgc atggtgagaa aacaagataa accactccca 2341 gcaccacctc ctcccttaag agatcctcct ccaccgccac ctgaaagacc tccaccaatc 2401 ccaccagaca atagactgag tagacacatc catcatgtgg aaagcgtgcc ttccagagac 2461 ccgccaatgc ctcttgaagc atggtgccct cgggatgtgt ttgggactaa tcagcttgtg 2521 ggatgtcgac tcctagggga gggctctcca aaacctggaa tcacagcgag ttcaaatgtc 2581 aatggaaggc acagtagagt gggctctgac ccagtgctta tgcggaaaca cagacgccat 2641 gatttgcctt tagaaggagc taaggtcttt tccaatggtc accttggaag tgaagaatat 2701 gatgttcctc cccggctttc tcctcctcct ccagttacca ccctcctccc tagcataaag 2761 tgtactggtc cgttagcaaa ttctctttca gagaaaacaa gagacccagt agaggaagat 2821 gatgatgaat acaagattcc ttcatcccac cctgtttccc tgaattcaca accatctcat 2881 tgtcataatg taaaacctcc tgttcggtct tgtgataatg gtcactgtat gctgaatgga 2941 acacatggtc catcttcaga gaagaaatca aacatccctg acttaagcat atatttaaag 3001 ggagatgttt ttgattcagc ctctgatccc gtgccattac cacctgccag gcctccaact 3061 cgggacaatc caaagcatgg ttcttcactc aacaggacgc cctctgatta tgatcttctc 3121 atccctccat taggtgaaga tgcttttgat gccctccctc catctctccc acctccccca 3181 cctcctgcaa ggcatagtct cattgaacat tcaaaacctc ctggctccag tagccggcca 3241 tcctcaggac aggatctttt tcttcttcct tcagatccct ttgttgatct agcaagtggc 3301 caagttcctt tgcctcctgc tagaaggtta ccaggtgaaa atgtcaaaac taacagaaca 3361 tcacaggact atgatcagct tccttcatgt tcagatggtt cacaggcacc agccagaccc 3421 cctaaaccac gaccgcgcag gactgcacca gaaattcacc acagaaaacc ccatgggcct 3481 gaggcggcat tggaaaatgt cgatgcaaaa attgcaaaac tcatgggaga gggttatgcc 3541 tttgaagagg tgaagagagc cttagagata gcccagaata atgtcgaagt tgcccggagc 3601 atcctccgag aatttgcctt ccctcctcca gtatccccac gtctaaatct aCTCGAGGAA 3661 CAAAAACTAA TATCAGAGGA AGATTTGGAA CAGAAACTAA TCAGTGAAGA AGATTTAGAG 3721 CAAAAGTTAA TTTCCGAGGA GGACTTATAG TGAGGGCCCG ATATCTCGAC AATCAACCTC 3781 TGGATTACAA AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC 3841 TATGTGGATA CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA 3901 TTTTCTCCTC CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG 3961 TCAGGCAACG TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA 4021 TTGCCACCAC CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG 4081 CGGAACTCAT CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG 4141 ACAATTCCGT GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG 4201 CCACCTGGAT TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG 4261 ACCTTCCTTC CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGCCTTCGCC 4321 CTCAGACGAG TCGGATCTCC CTTTGGGCCG CCTCCCCGCC TGGAAACGGG GGAGGCTAAC 4381 TGAAACACGG AAGGAGACAA TACCGGAAGG AACCCGCGCT ATGACGGCAA TAAAAAGACA 4441 GAATAAAACG CACGGGTGTT GGGTCGTTTG TTCATAAACG CGGGGTTCGG TCCCAGGGCT 4501 GGCACTCTGT CGATACCCCA CCGAGACCCC ATTGGGGCCA ATACGCCCGC GTTTCTTCCT 4561 TTTCCCCACC CCACCCCCCA AGTTCGGGTG AAGGCCCAGG GCTCGCAGCC AACGTCGGGG 4621 CGGCAGGCCC TGCCATAGCA GATCTGCGCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC 4681 CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC 4741 TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG 4801 CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GCATCCCTTT AGGGTTCCGA TTTAGTGCTT 4861 TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC 4921 CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT 4981 TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA 5041 TTTTGGGGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA 5101 ATTAATTCTG TGGAATGTGT GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG 5161 CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG 5221 CTCCCCAGCA GGCAGAAGTA TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC 5281 GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA 5341 TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT 5401 CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC 5461 TTGTATATCC ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA 5521 ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA 5581 CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG 5641 GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA 5701 GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT 5761 TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT 5821 GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT 5881 GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG 5941 AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA 6001 GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA 6061 TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT 6121 TTCTGGATTC ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT 6181 GGCTACCCGT GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT 6241 TTACGGTATC GCCGCTCCCG ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT 6301 CTTCTGAGCG GGACTCTGGG GTTCGCGAAA TGACCGACCA AGCGACGCCC AACCTGCCAT 6361 CACGAGATTT CGATTCCACC GCCGCCTTCT ATGAAAGGTT GGGCTTCGGA ATCGTTTTCC 6421 GGGACGCCGG CTGGATGATC CTCCAGCGCG GGGATCTCAT GCTGGAGTTC TTCGCCCACC 6481 CCAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA 6541 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT 6601 CTTATCATGT CTGTATACCG TCGACCTCTA GCTAGAGCTT GGCGTAATCA TGGTCATAGC 6661 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA 6721 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT 6781 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC 6841 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 6901 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 6961 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 7021 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 7081 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 7141 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 7201 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAA TGCTCACGCT 7261 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 7321 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 7381 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 7441 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG 7501 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 7561 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA 7621 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 7681 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 7741 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 7801 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 7861 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 7921 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 7981 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 8041 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 8101 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 8161 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 8221 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 8281 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 8341 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 8401 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 8461 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 8521 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 8581 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 8641 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 8701 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 8761 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC GACGGATCGG 8821 GAGATCTCCC GATCCCCTAT GGTCGACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT 8881 AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG CGAGCAAAAT 8941 TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC TTAGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.