pLenti-U6-PDE6G-sgRNA2-Cas9-Pu

pLenti-U6-PDE6G-sgRNA2-Cas9-Puro plasmid

PVT94794

pLenti-U6-PDE6G-sgRNA2-Cas9-Puro plasmid Information

Plasmid name: pLenti-U6-PDE6G(human)-sgRNA2-Cas9-Puro
Alias: TGAACCTGGAACCGCCCAAGG
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Gene editing
Fragment type: CRISPR
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLenti-U6-PDE6G-sgRNA2-Cas9-Puro plasmid Description

pLenti-U6-PDE6G-sgRNA2-Cas9-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLenti-U6-PDE6G-sgRNA2-Cas9-Puro plasmid Sequence

1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg 61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt 121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc 181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac 241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat 301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg 361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt 421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag 481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc 541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag 601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt 661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc 721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg 781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct 841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt 901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac 961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc 1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc 1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa 1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg 1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata 1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc 1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga 1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc 1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca 1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg 1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga 1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata 1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg 1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg 1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag 1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt 1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt 1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt 2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag 2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata 2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta 2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta 2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa 2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt 2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat 2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa 2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag 2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg 2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag 2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta 2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc 2821 ttggctttat atatcttGTG GAAAGGACGA AACACCgTGA ACCTGGAACC GCCCAAGGgt 2881 tttagagcta GAAAtagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg 2941 caccgagtcg gtgcTTTTTT gaattcgcta gctaggtctt gaaaggagtg ggaattggct 3001 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 3061 gggtcggcaa ttgatccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 3121 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 3181 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggac cggttctaga 3241 gcgctgccac cATGGACAAG AAGTACAGCA TCGGCCTGGA CATCGGCACC AACTCTGTGG 3301 GCTGGGCCGT GATCACCGAC GAGTACAAGG TGCCCAGCAA GAAATTCAAG GTGCTGGGCA 3361 ACACCGACCG GCACAGCATC AAGAAGAACC TGATCGGAGC CCTGCTGTTC GACAGCGGCG 3421 AAACAGCCGA GGCCACCCGG CTGAAGAGAA CCGCCAGAAG AAGATACACC AGACGGAAGA 3481 ACCGGATCTG CTATCTGCAA GAGATCTTCA GCAACGAGAT GGCCAAGGTG GACGACAGCT 3541 TCTTCCACAG ACTGGAAGAG TCCTTCCTGG TGGAAGAGGA TAAGAAGCAC GAGCGGCACC 3601 CCATCTTCGG CAACATCGTG GACGAGGTGG CCTACCACGA GAAGTACCCC ACCATCTACC 3661 ACCTGAGAAA GAAACTGGTG GACAGCACCG ACAAGGCCGA CCTGCGGCTG ATCTATCTGG 3721 CCCTGGCCCA CATGATCAAG TTCCGGGGCC ACTTCCTGAT CGAGGGCGAC CTGAACCCCG 3781 ACAACAGCGA CGTGGACAAG CTGTTCATCC AGCTGGTGCA GACCTACAAC CAGCTGTTCG 3841 AGGAAAACCC CATCAACGCC AGCGGCGTGG ACGCCAAGGC CATCCTGTCT GCCAGACTGA 3901 GCAAGAGCAG ACGGCTGGAA AATCTGATCG CCCAGCTGCC CGGCGAGAAG AAGAATGGCC 3961 TGTTCGGAAA CCTGATTGCC CTGAGCCTGG GCCTGACCCC CAACTTCAAG AGCAACTTCG 4021 ACCTGGCCGA GGATGCCAAA CTGCAGCTGA GCAAGGACAC CTACGACGAC GACCTGGACA 4081 ACCTGCTGGC CCAGATCGGC GACCAGTACG CCGACCTGTT TCTGGCCGCC AAGAACCTGT 4141 CCGACGCCAT CCTGCTGAGC GACATCCTGA GAGTGAACAC CGAGATCACC AAGGCCCCCC 4201 TGAGCGCCTC TATGATCAAG AGATACGACG AGCACCACCA GGACCTGACC CTGCTGAAAG 4261 CTCTCGTGCG GCAGCAGCTG CCTGAGAAGT ACAAAGAGAT TTTCTTCGAC CAGAGCAAGA 4321 ACGGCTACGC CGGCTACATT GACGGCGGAG CCAGCCAGGA AGAGTTCTAC AAGTTCATCA 4381 AGCCCATCCT GGAAAAGATG GACGGCACCG AGGAACTGCT CGTGAAGCTG AACAGAGAGG 4441 ACCTGCTGCG GAAGCAGCGG ACCTTCGACA ACGGCAGCAT CCCCCACCAG ATCCACCTGG 4501 GAGAGCTGCA CGCCATTCTG CGGCGGCAGG AAGATTTTTA CCCATTCCTG AAGGACAACC 4561 GGGAAAAGAT CGAGAAGATC CTGACCTTCC GCATCCCCTA CTACGTGGGC CCTCTGGCCA 4621 GGGGAAACAG CAGATTCGCC TGGATGACCA GAAAGAGCGA GGAAACCATC ACCCCCTGGA 4681 ACTTCGAGGA AGTGGTGGAC AAGGGCGCTT CCGCCCAGAG CTTCATCGAG CGGATGACCA 4741 ACTTCGATAA GAACCTGCCC AACGAGAAGG TGCTGCCCAA GCACAGCCTG CTGTACGAGT 4801 ACTTCACCGT GTATAACGAG CTGACCAAAG TGAAATACGT GACCGAGGGA ATGAGAAAGC 4861 CCGCCTTCCT GAGCGGCGAG CAGAAAAAGG CCATCGTGGA CCTGCTGTTC AAGACCAACC 4921 GGAAAGTGAC CGTGAAGCAG CTGAAAGAGG ACTACTTCAA GAAAATCGAG TGCTTCGACT 4981 CCGTGGAAAT CTCCGGCGTG GAAGATCGGT TCAACGCCTC CCTGGGCACA TACCACGATC 5041 TGCTGAAAAT TATCAAGGAC AAGGACTTCC TGGACAATGA GGAAAACGAG GACATTCTGG 5101 AAGATATCGT GCTGACCCTG ACACTGTTTG AGGACAGAGA GATGATCGAG GAACGGCTGA 5161 AAACCTATGC CCACCTGTTC GACGACAAAG TGATGAAGCA GCTGAAGCGG CGGAGATACA 5221 CCGGCTGGGG CAGGCTGAGC CGGAAGCTGA TCAACGGCAT CCGGGACAAG CAGTCCGGCA 5281 AGACAATCCT GGATTTCCTG AAGTCCGACG GCTTCGCCAA CAGAAACTTC ATGCAGCTGA 5341 TCCACGACGA CAGCCTGACC TTTAAAGAGG ACATCCAGAA AGCCCAGGTG TCCGGCCAGG 5401 GCGATAGCCT GCACGAGCAC ATTGCCAATC TGGCCGGCAG CCCCGCCATT AAGAAGGGCA 5461 TCCTGCAGAC AGTGAAGGTG GTGGACGAGC TCGTGAAAGT GATGGGCCGG CACAAGCCCG 5521 AGAACATCGT GATCGAAATG GCCAGAGAGA ACCAGACCAC CCAGAAGGGA CAGAAGAACA 5581 GCCGCGAGAG AATGAAGCGG ATCGAAGAGG GCATCAAAGA GCTGGGCAGC CAGATCCTGA 5641 AAGAACACCC CGTGGAAAAC ACCCAGCTGC AGAACGAGAA GCTGTACCTG TACTACCTGC 5701 AGAATGGGCG GGATATGTAC GTGGACCAGG AACTGGACAT CAACCGGCTG TCCGACTACG 5761 ATGTGGACCA TATCGTGCCT CAGAGCTTTC TGAAGGACGA CTCCATCGAC AACAAGGTGC 5821 TGACCAGAAG CGACAAGAAC CGGGGCAAGA GCGACAACGT GCCCTCCGAA GAGGTCGTGA 5881 AGAAGATGAA GAACTACTGG CGGCAGCTGC TGAACGCCAA GCTGATTACC CAGAGAAAGT 5941 TCGACAATCT GACCAAGGCC GAGAGAGGCG GCCTGAGCGA ACTGGATAAG GCCGGCTTCA 6001 TCAAGAGACA GCTGGTGGAA ACCCGGCAGA TCACAAAGCA CGTGGCACAG ATCCTGGACT 6061 CCCGGATGAA CACTAAGTAC GACGAGAATG ACAAGCTGAT CCGGGAAGTG AAAGTGATCA 6121 CCCTGAAGTC CAAGCTGGTG TCCGATTTCC GGAAGGATTT CCAGTTTTAC AAAGTGCGCG 6181 AGATCAACAA CTACCACCAC GCCCACGACG CCTACCTGAA CGCCGTCGTG GGAACCGCCC 6241 TGATCAAAAA GTACCCTAAG CTGGAAAGCG AGTTCGTGTA CGGCGACTAC AAGGTGTACG 6301 ACGTGCGGAA GATGATCGCC AAGAGCGAGC AGGAAATCGG CAAGGCTACC GCCAAGTACT 6361 TCTTCTACAG CAACATCATG AACTTTTTCA AGACCGAGAT TACCCTGGCC AACGGCGAGA 6421 TCCGGAAGCG GCCTCTGATC GAGACAAACG GCGAAACCGG GGAGATCGTG TGGGATAAGG 6481 GCCGGGATTT TGCCACCGTG CGGAAAGTGC TGAGCATGCC CCAAGTGAAT ATCGTGAAAA 6541 AGACCGAGGT GCAGACAGGC GGCTTCAGCA AAGAGTCTAT CCTGCCCAAG AGGAACAGCG 6601 ATAAGCTGAT CGCCAGAAAG AAGGACTGGG ACCCTAAGAA GTACGGCGGC TTCGACAGCC 6661 CCACCGTGGC CTATTCTGTG CTGGTGGTGG CCAAAGTGGA AAAGGGCAAG TCCAAGAAAC 6721 TGAAGAGTGT GAAAGAGCTG CTGGGGATCA CCATCATGGA AAGAAGCAGC TTCGAGAAGA 6781 ATCCCATCGA CTTTCTGGAA GCCAAGGGCT ACAAAGAAGT GAAAAAGGAC CTGATCATCA 6841 AGCTGCCTAA GTACTCCCTG TTCGAGCTGG AAAACGGCCG GAAGAGAATG CTGGCCTCTG 6901 CCGGCGAACT GCAGAAGGGA AACGAACTGG CCCTGCCCTC CAAATATGTG AACTTCCTGT 6961 ACCTGGCCAG CCACTATGAG AAGCTGAAGG GCTCCCCCGA GGATAATGAG CAGAAACAGC 7021 TGTTTGTGGA ACAGCACAAG CACTACCTGG ACGAGATCAT CGAGCAGATC AGCGAGTTCT 7081 CCAAGAGAGT GATCCTGGCC GACGCTAATC TGGACAAAGT GCTGTCCGCC TACAACAAGC 7141 ACCGGGATAA GCCCATCAGA GAGCAGGCCG AGAATATCAT CCACCTGTTT ACCCTGACCA 7201 ATCTGGGAGC CCCTGCCGCC TTCAAGTACT TTGACACCAC CATCGACCGG AAGAGGTACA 7261 CCAGCACCAA AGAGGTGCTG GACGCCACCC TGATCCACCA GAGCATCACC GGCCTGTACG 7321 AGACACGGAT CGACCTGTCT CAGCTGGGAG GCGACAAGCG ACCTGCCGCC ACAAAGAAGG 7381 CTGGACAGGC TAAGAAGAAG AAAGATTACA AAGACGATGA CGATAAGGGA TCCGGCGCAA 7441 CAAACTTCTC TCTGCTGAAA CAAGCCGGAG ATGTCGAAGA GAATCCTGGA CCGACCGAGT 7501 ACAAGCCCAC GGTGCGCCTC GCCACCCGCG ACGACGTCCC CAGGGCCGTA CGCACCCTCG 7561 CCGCCGCGTT CGCCGACTAC CCCGCCACGC GCCACACCGT CGATCCGGAC CGCCACATCG 7621 AGCGGGTCAC CGAGCTGCAA GAACTCTTCC TCACGCGCGT CGGGCTCGAC ATCGGCAAGG 7681 TGTGGGTCGC GGACGACGGC GCCGCGGTGG CGGTCTGGAC CACGCCGGAG AGCGTCGAAG 7741 CGGGGGCGGT GTTCGCCGAG ATCGGCCCGC GCATGGCCGA GTTGAGCGGT TCCCGGCTGG 7801 CCGCGCAGCA ACAGATGGAA GGCCTCCTGG CGCCGCACCG GCCCAAGGAG CCCGCGTGGT 7861 TCCTGGCCAC CGTCGGAGTC TCGCCCGACC ACCAGGGCAA GGGTCTGGGC AGCGCCGTCG 7921 TGCTCCCCGG AGTGGAGGCG GCCGAGCGCG CCGGGGTGCC CGCCTTCCTG GAGACCTCCG 7981 CGCCCCGCAA CCTCCCCTTC TACGAGCGGC TCGGCTTCAC CGTCACCGCC GACGTCGAGG 8041 TGCCCGAAGG ACCGCGCACC TGGTGCATGA CCCGCAAGCC CGGTGCCTGA ACGCGTTAAG 8101 TCGACAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT GACTGGTATT CTTAACTATG 8161 TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC TTTGTATCAT GCTATTGCTT 8221 CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG GTTGCTGTCT CTTTATGAGG 8281 AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC TGTGTTTGCT GACGCAACCC 8341 CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC CGGGACTTTC GCTTTCCCCC 8401 TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC CCGCTGCTGG ACAGGGGCTC 8461 GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA ATCATCGTCC TTTCCTTGGC 8521 TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC CTTCTGCTAC GTCCCTTCGG 8581 CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC GGCTCTGCGG CCTCTTCCGC 8641 GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG GGCCGCCTCC CCGCGTCGAC 8701 TTTAAGACCA ATGACTTACA AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG 8761 GGGACTGGAA GGGCTAATTC ACTCCCAACG AAGACAAGAT CTGCTTTTTG CTTGTACTGG 8821 GTCTCTCTGG TTAGACCAGA TCTGAGCCTG GGAGCTCTCT GGCTAACTAG GGAACCCACT 8881 GCTTAAGCCT CAATAAAGCT TGCCTTGAGT GCTTCAAGTA GTGTGTGCCC GTCTGTTGTG 8941 TGACTCTGGT AACTAGAGAT CCCTCAGACC CTTTTAGTCA GTGTGGAAAA TCTCTAGCAG 9001 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 9061 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 9121 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 9181 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 9241 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc 9301 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 9361 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 9421 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 9481 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 9541 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 9601 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 9661 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 9721 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 9781 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 9841 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 9901 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 9961 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 10021 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 10081 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca 10141 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc 10201 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac 10261 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac 10321 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg 10381 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac 10441 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag 10501 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga 10561 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc 10621 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc 10681 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 10741 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 10801 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg 10861 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc 10921 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg 10981 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc 11041 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact 11101 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 11161 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 11221 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 11281 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 11341 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 11401 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 11461 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 11521 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 11581 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 11641 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 11701 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 11761 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 11821 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 11881 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 11941 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 12001 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 12061 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 12121 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 12181 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 12241 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 12301 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg 12361 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 12421 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 12481 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 12541 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 12601 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat 12661 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 12721 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 12781 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 12841 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 12901 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag 12961 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgac

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.