pCMV-Sorbs2-3×FLAG-Neo plasmid

pCMV-Sorbs2-3×FLAG-Neo plasmid

PVT94769

pCMV-Sorbs2-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-Sorbs2(rat)-3×FLAG-Neo
Alias: NM_053770.3;Argbp2
Gene lenght: 3588bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: WPRE-R
Copy number: High
Induction:

pCMV-Sorbs2-3×FLAG-Neo plasmid Description

pCMV-Sorbs2-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Sorbs2-3×FLAG-Neo plasmid Sequence

1 GTTAGGCGTT TTGCGCTGCT TCGCGATGTA CGGGCCAGAT ATACGCGTTG ACATTGATTA 61 TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC ATATATGGAG 121 TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA CGACCCCCGC 181 CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC TTTCCATTGA 241 CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA AGTGTATCAT 301 ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG GCATTATGCC 361 CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT AGTCATCGCT 421 ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG GTTTGACTCA 481 CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG GCACCAAAAT 541 CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT GGGCGGTAGG 601 CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT 661 TACTGGCTTA TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA 721 AACTTAAGCT TGGTACCGAG CTCGAATTCG CCACCatgaa tacagatagc ggtgggtgtg 781 ctcgcaaacg tgccgccatg tctgtcacgt taacatctgt gaagagagtt caaagttctc 841 caaacctatt ggctgcaggg cgtgagtctc actctccaga ctcagcttgg agatcttaca 901 atggtcgaaa tccagagaca ctgaacggag atgccacata ttcctctctt gcagcaaaag 961 gttttagaag cgttcgacca aacctgcaag acaaaaagtc accaacccag agccatatca 1021 ctatcaacgg caactctggt ggcgccgtga gtccagtgag ttactatcag aggccattct 1081 ccccttctgc atactccctc ccagcctcac tcaactccag cattatcatg ccacacggca 1141 ggtcgcttga ttctgcggag acatattccc agcatgccca gtcgctagac ggcaccatgg 1201 gaagctccat cccactctac agatcctccg aggaagagaa gagggtcaca gtcatcaaag 1261 ccccgcatta cccagggatc ggccctgtgg atgagtctgg aatccccaca gccattagaa 1321 cgacagttga ccggccgaag gactggtaca agacaatgtt taaacaaatt cacatggtac 1381 acaagccgga tgaggacaca gacatgtata atactcctta cacatacaat gcaggtctgt 1441 acaactcgcc ctacagtgct cagtcacatc ctgctgcaaa gacccagacc tacagacctc 1501 tttccaaaag ccactcggac aatggcaccg atgcttttaa ggaggcaccc tcaccagtgc 1561 ctcccccaca cgttccacca cgaccaagag atcagtcttc aacagaaaag catgactggg 1621 atcccccaga cagaaaggtg gacaccagga aatttcgatc ggagccaagg agtatttttg 1681 aatacgagcc tgggaagtca tccatcctgc agcacgaaag acccgtctcc gtctaccagt 1741 cttccataga cagaagcttg gaaagaccca gcagctctgc aagcatggcg ggtgacttta 1801 gaaaacggag gaagagtgaa cctgcagtgg gcccgcccag gggcttgggg gatcacagtt 1861 caagcaggac cagccccggc cgggcagacc tcccaggatc aagttccacc tttaccacgt 1921 ctttcattag ttcttctcct tcctctccct cgagagcaca aggtggggat gatagcaaaa 1981 tgtgtccgcc cctttgcagt tactcggggc tcaatggctc gccctctagt gagttagagt 2041 gctgcggcgc ttatagaagg cacttggacg tcccccagga ctctcaaagg gccatcactt 2101 tcaagaacgg ctggcaaatg gcccggcaaa atgcagagat ctggagtagc actgaagagg 2161 cggtttcccc caaaatcaaa tcacgaagct gtgacgatct cctgaatgat gactgcggca 2221 gcttcccaga ccctaaaacc aagtcagaaa gcatgggttc tctgttatgt gacgaaggct 2281 ccaaagagag cgaccccatg acgtggactt ccccctacat cccggaagtg tgcgggaaca 2341 gcagatctag gctcaaacat aggtcagccc ataacgcccc aggcttcctc aaaatgtaca 2401 agaaaatgca ccgcatcaac cgcaaggatt tgatgaactc ggaggtcatt tgctctgtga 2461 aatccaggat ccttcagtac gagaaggaac agcagcacag ggggctgctc catggatgga 2521 gccagtcgtc caccgaggag gtgcccaggg acgtggtacc cactcgcatc tcggagtttg 2581 agaagctgat tcagaagtca aagtctatgc ccaatctagg agatgaaatg ttatctcctg 2641 taaccctaga acccccacaa aatggtttgt gccccaagag gcgattttct attgagtctc 2701 tgctggagga ggaaactcag gtccgacacc cttctcaggg tcagcgaagc tgcaagtcga 2761 acaccctggt acccatccac atcgaggtca ccagcgatga gcaacctaga acacatatgg 2821 agttttccga cagtgaccaa gatggggttg tgtctgacca cagcgactac gtccacgtcg 2881 aagggtcgtc cttttgtagt gaaagtgact tcgaccactt ttcattcaca tcctctgaaa 2941 gtttctacgg atccagccat caccaccacc atcaccacca ccatcaccga cacctcatca 3001 gttcctgcaa aggccgatgc cccgcttctt acactcgatt taccacgatg ttaaaacacg 3061 aaagagctaa gcatgaaaat attgaccgac ccagaaggca agacatggat cctggcctat 3121 ctaaactcgc gtttctagtc agccctgtgc ctttccgaag gaaaaaagtt ttgactcccc 3181 aaaaacaaac tgagcaggca aaatgcaaag cctcggtagt tgaggctctg gactctgccc 3241 ttaaagacat ttgcgaccaa ataaaagctg aaaagcggag aggaagcttg ccggacaaca 3301 gcatcctgca caggcttatt agtgaactgc tgccacagat tcctgaaagg aattcatctc 3361 ttaatgctct aaaaaggagc cccatgcacc agcctttcca cccactgcct caagatggtg 3421 ctattcattg tcccctgtac caaaatgatt gtgggagaat gcctcacagt gcctctttcc 3481 cagacgtgga cacgaccagc agctaccacg cacaggacta tggtagtgtg ctgagtctcc 3541 aagatcacga gtcccctaga agttactcgt ctactctgac tgacttggga agaagtgtat 3601 cacgggaacg aagaggaact ccagaaaaag aggtaaaatt gcctgcaaaa gctgtctatg 3661 atttcaaagc tcagacttct aaggagctgt catttaagaa aggagacacc gtctacatcc 3721 tcaggaaaat tgaccagaac tggtatgagg gggagcacca cggaagagtg ggcattttcc 3781 caatctcata cgtagagaaa ctaacacccc cagaaaaagc gcagcccgcg agaccaccac 3841 ccccagtcca gccgggagag attggagaag ccatagccaa gtacaacttc aatgcagaca 3901 caaatgtgga actctccctg agaaagggtg acaggattat tcttctcaaa agagttgatc 3961 aaaactggta tgaaggtaaa atcccaggaa ccaacagaca aggcatcttc cctgtctcct 4021 acgtagaagt tgtcaagagg aacacgaaag gttctgagga ttaccccgac cctcctctac 4081 cccacagcta ctccagtgat agaatttaca gcctaagctc caataagcca cagcgtcctg 4141 tgttctctca cgaaaacatt caaggtggag gagaaccgtt tcaggctctg tataactata 4201 ctcctaggaa tgaagatgag ctggaactca gagaaagtga tgtcgtagat gtcatggaaa 4261 agtgtgatga cggatggttc gtgggaactt caagaagaac caaattcttt ggtacttttc 4321 ctggaaacta tgtcaaaagg ctgGGATCCG ACTACAAAGA CCATGACGGT GATTATAAAG 4381 ATCATGACAT CGACTACAAG GATGACGATG ACAAGTAGTG AGGGCCCGAT ATCTCGACAA 4441 TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC 4501 TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT 4561 GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG 4621 GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG 4681 TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT 4741 TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT 4801 GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAGCTGACG TCCTTTCCAT GGCTGCTCGC 4861 CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA 4921 TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG 4981 CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCCTG GAAACGGGGG 5041 AGGCTAACTG AAACACGGAA GGAGACAATA CCGGAAGGAA CCCGCGCTAT GACGGCAATA 5101 AAAAGACAGA ATAAAACGCA CGGGTGTTGG GTCGTTTGTT CATAAACGCG GGGTTCGGTC 5161 CCAGGGCTGG CACTCTGTCG ATACCCCACC GAGACCCCAT TGGGGCCAAT ACGCCCGCGT 5221 TTCTTCCTTT TCCCCACCCC ACCCCCCAAG TTCGGGTGAA GGCCCAGGGC TCGCAGCCAA 5281 CGTCGGGGCG GCAGGCCCTG CCATAGCAGA TCTGCGCAGC TGGGGCTCTA GGGGGTATCC 5341 CCACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC 5401 CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC 5461 CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGC ATCCCTTTAG GGTTCCGATT 5521 TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG 5581 GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG 5641 TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT 5701 ATAAGGGATT TTGGGGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT 5761 TAACGCGAAT TAATTCTGTG GAATGTGTGT CAGTTAGGGT GTGGAAAGTC CCCAGGCTCC 5821 CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT CAGCAACCAG GTGTGGAAAG 5881 TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC ATCTCAATTA GTCAGCAACC 5941 ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT 6001 CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCTGCCTCT 6061 GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC TAGGCTTTTG CAAAAAGCTC 6121 CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC 6181 ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC 6241 GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA 6301 GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG 6361 CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG 6421 CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG 6481 GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG 6541 CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC 6601 ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA 6661 GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC 6721 GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT 6781 GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC 6841 ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC 6901 CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT 6961 GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGCGAAATG ACCGACCAAG CGACGCCCAA 7021 CCTGCCATCA CGAGATTTCG ATTCCACCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT 7081 CGTTTTCCGG GACGCCGGCT GGATGATCCT CCAGCGCGGG GATCTCATGC TGGAGTTCTT 7141 CGCCCACCCC AACTTGTTTA TTGCAGCTTA TAATGGTTAC AAATAAAGCA ATAGCATCAC 7201 AAATTTCACA AATAAAGCAT TTTTTTCACT GCATTCTAGT TGTGGTTTGT CCAAACTCAT 7261 CAATGTATCT TATCATGTCT GTATACCGTC GACCTCTAGC TAGAGCTTGG CGTAATCATG 7321 GTCATAGCTG TTTCCTGTGT GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC 7381 CGGAAGCATA AAGTGTAAAG CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC 7441 GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT 7501 CGGCCAACGC GCGGGGAGAG GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC 7561 TGACTCGCTG CGCTCGGTCG TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 7621 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 7681 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 7741 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 7801 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 7861 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCAATG 7921 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 7981 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 8041 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 8101 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 8161 AAGGACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 8221 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 8281 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 8341 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 8401 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 8461 TGAGTAAACT TGGTCTGACA GTTACCAATG CTTAATCAGT GAGGCACCTA TCTCAGCGAT 8521 CTGTCTATTT CGTTCATCCA TAGTTGCCTG ACTCCCCGTC GTGTAGATAA CTACGATACG 8581 GGAGGGCTTA CCATCTGGCC CCAGTGCTGC AATGATACCG CGAGACCCAC GCTCACCGGC 8641 TCCAGATTTA TCAGCAATAA ACCAGCCAGC CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC 8701 AACTTTATCC GCCTCCATCC AGTCTATTAA TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC 8761 GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC CATTGCTACA GGCATCGTGG TGTCACGCTC 8821 GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG TTCCCAACGA TCAAGGCGAG TTACATGATC 8881 CCCCATGTTG TGCAAAAAAG CGGTTAGCTC CTTCGGTCCT CCGATCGTTG TCAGAAGTAA 8941 GTTGGCCGCA GTGTTATCAC TCATGGTTAT GGCAGCACTG CATAATTCTC TTACTGTCAT 9001 GCCATCCGTA AGATGCTTTT CTGTGACTGG TGAGTACTCA ACCAAGTCAT TCTGAGAATA 9061 GTGTATGCGG CGACCGAGTT GCTCTTGCCC GGCGTCAATA CGGGATAATA CCGCGCCACA 9121 TAGCAGAACT TTAAAAGTGC TCATCATTGG AAAACGTTCT TCGGGGCGAA AACTCTCAAG 9181 GATCTTACCG CTGTTGAGAT CCAGTTCGAT GTAACCCACT CGTGCACCCA ACTGATCTTC 9241 AGCATCTTTT ACTTTCACCA GCGTTTCTGG GTGAGCAAAA ACAGGAAGGC AAAATGCCGC 9301 AAAAAAGGGA ATAAGGGCGA CACGGAAATG TTGAATACTC ATACTCTTCC TTTTTCAATA 9361 TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA 9421 GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGTCGA 9481 CGGATCGGGA GATCTCCCGA TCCCCTATGG TCGACTCTCA GTACAATCTG CTCTGATGCC 9541 GCATAGTTAA GCCAGTATCT GCTCCCTGCT TGTGTGTTGG AGGTCGCTGA GTAGTGCGCG 9601 AGCAAAATTT AAGCTACAAC AAGGCAAGGC TTGACCGACA ATTGCATGAA GAATCTGCTT 9661 AGG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.