pGL4.75-OpIE-2-hRluc plasmid

pGL4.75-OpIE-2-hRluc plasmid

PVT94761

pGL4.75-OpIE-2-hRluc plasmid Information

Plasmid name: pGL4.75-OpIE-2-hRluc
Alias:
Gene lenght:
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc,Rluc
Promoter: OplE2
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: RVP3
Reverse primer:
Copy number: High
Induction:

pGL4.75-OpIE-2-hRluc plasmid Description

pGL4.75-OpIE-2-hRluc is a plasmid suitable for promoter detection in mammalian cells.

pGL4.75-OpIE-2-hRluc plasmid Sequence

1 GGCCTAACTG GCCTCAATAT TGGCCATTAG CCATATTATT CATTGGTTAT ATAGCATAAA 61 TCAATATTGG CTATTGGCCA TTGCATACGT TGTATCTATA TCATAATATG TACACATGAT 121 GATAAACAAT GTATGGTGCT AATGTTGCTT CAACAACAAT TCTGTTGAAC TGTGTTTTCA 181 TGTTTGCCAA CAAGCACCTT TATACTCGGT GGCCTCCCCA CCACCAACTT TTTTGCACTG 241 CAAAAAAACA CGCTTTTGCA CGCGGGCCCA TACATAGTAC AAACTCTACG TTTCGTAGAC 301 TATTTTACAT AAATAGTCTA CACCGTTGTA TACGCTCCAA ATACACTACC ACACATTGAA 361 CCTTTTTGCA GTGCAAAAAA GTACGTGTCG GCAGTCACGT AGGCCGGCCT TATCGGGTCG 421 CGTCCTGTCA CGTACGAATC ACATTATCGG ACCGGACGAG TGTTGTCTTA TCGTGACAGG 481 ACGCCAGCTT CCTGTGTTGC TAACCGCAGC CGGACGCAAC TCCTTATCGG AACAGGACGC 541 GCCTCCATAT CAGCCGCGCG TTATCTCATG CGCGTGACCG GACACGAGGC GCCCGTCCCG 601 CTTATCGCGC CTATAAATAC AGCCCGCAAC GATCTGGTAA ACACAGTTGA ACAGCATCTG 661 TTCGTTTAGT GAACCGTCAG ATCACTAGAA GCTTTATTGC GGTAGTTTAT CACAGTTAAA 721 TTGCTAACGC AGTCAGTGGG CCTCGGCGGC CAAGCTTGGC AATCCGGTAC TGTTGGTAAA 781 GCCACCATGG CTTCCAAGGT GTACGACCCC GAGCAACGCA AACGCATGAT CACTGGGCCT 841 CAGTGGTGGG CTCGCTGCAA GCAAATGAAC GTGCTGGACT CCTTCATCAA CTACTATGAT 901 TCCGAGAAGC ACGCCGAGAA CGCCGTGATT TTTCTGCATG GTAACGCTGC CTCCAGCTAC 961 CTGTGGAGGC ACGTCGTGCC TCACATCGAG CCCGTGGCTA GATGCATCAT CCCTGATCTG 1021 ATCGGAATGG GTAAGTCCGG CAAGAGCGGG AATGGCTCAT ATCGCCTCCT GGATCACTAC 1081 AAGTACCTCA CCGCTTGGTT CGAGCTGCTG AACCTTCCAA AGAAAATCAT CTTTGTGGGC 1141 CACGACTGGG GGGCTTGTCT GGCCTTTCAC TACTCCTACG AGCACCAAGA CAAGATCAAG 1201 GCCATCGTCC ATGCTGAGAG TGTCGTGGAC GTGATCGAGT CCTGGGACGA GTGGCCTGAC 1261 ATCGAGGAGG ATATCGCCCT GATCAAGAGC GAAGAGGGCG AGAAAATGGT GCTTGAGAAT 1321 AACTTCTTCG TCGAGACCAT GCTCCCAAGC AAGATCATGC GGAAACTGGA GCCTGAGGAG 1381 TTCGCTGCCT ACCTGGAGCC ATTCAAGGAG AAGGGCGAGG TTAGACGGCC TACCCTCTCC 1441 TGGCCTCGCG AGATCCCTCT CGTTAAGGGA GGCAAGCCCG ACGTCGTCCA GATTGTCCGC 1501 AACTACAACG CCTACCTTCG GGCCAGCGAC GATCTGCCTA AGATGTTCAT CGAGTCCGAC 1561 CCTGGGTTCT TTTCCAACGC TATTGTCGAG GGAGCTAAGA AGTTCCCTAA CACCGAGTTC 1621 GTGAAGGTGA AGGGCCTCCA CTTCAGCCAG GAGGACGCTC CAGATGAAAT GGGTAAGTAC 1681 ATCAAGAGCT TCGTGGAGCG CGTGCTGAAG AACGAGCAGT AATTCTAGAG TCGGGGCGGC 1741 CGGCCGCTTC GAGCAGACAT GATAAGATAC ATTGATGAGT TTGGACAAAC CACAACTAGA 1801 ATGCAGTGAA AAAAATGCTT TATTTGTGAA ATTTGTGATG CTATTGCTTT ATTTGTAACC 1861 ATTATAAGCT GCAATAAACA AGTTAACAAC AACAATTGCA TTCATTTTAT GTTTCAGGTT 1921 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTAAAATC 1981 GATAAGGATC CGTCGACCGA TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG 2041 GGCGCGGGGC ATGACTATCG TCGCCGCACT TATGACTGTC TTCTTTATCA TGCAACTCGT 2101 AGGACAGGTG CCGGCAGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG 2161 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT 2221 CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA 2281 AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA 2341 ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC 2401 CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT 2461 CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA 2521 GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG 2581 ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT 2641 CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA 2701 CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT 2761 GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC 2821 AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA 2881 AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA 2941 ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT 3001 TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA 3061 GCGGCCGCAA ATGCTAAACC ACTGCAGTGG TTACCAGTGC TTGATCAGTG AGGCACCGAT 3121 CTCAGCGATC TGCCTATTTC GTTCGTCCAT AGTGGCCTGA CTCCCCGTCG TGTAGATCAC 3181 TACGATTCGT GAGGGCTTAC CATCAGGCCC CAGCGCAGCA ATGATGCCGC GAGAGCCGCG 3241 TTCACCGGCC CCCGATTTGT CAGCAATGAA CCAGCCAGCA GGGAGGGCCG AGCGAAGAAG 3301 TGGTCCTGCT ACTTTGTCCG CCTCCATCCA GTCTATGAGC TGCTGTCGTG ATGCTAGAGT 3361 AAGAAGTTCG CCAGTGAGTA GTTTCCGAAG AGTTGTGGCC ATTGCTACTG GCATCGTGGT 3421 ATCACGCTCG TCGTTCGGTA TGGCTTCGTT CAACTCTGGT TCCCAGCGGT CAAGCCGGGT 3481 CACATGATCA CCCATATTAT GAAGAAATGC AGTCAGCTCC TTAGGGCCTC CGATCGTTGT 3541 CAGAAGTAAG TTGGCCGCGG TGTTGTCGCT CATGGTAATG GCAGCACTAC ACAATTCTCT 3601 TACCGTCATG CCATCCGTAA GATGCTTTTC CGTGACCGGC GAGTACTCAA CCAAGTCGTT 3661 TTGTGAGTAG TGTATACGGC GACCAAGCTG CTCTTGCCCG GCGTCTATAC GGGACAACAC 3721 CGCGCCACAT AGCAGTACTT TGAAAGTGCT CATCATCGGG AATCGTTCTT CGGGGCGGAA 3781 AGACTCAAGG ATCTTGCCGC TATTGAGATC CAGTTCGATA TAGCCCACTC TTGCACCCAG 3841 TTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCGGGG TGTGCAAAAA CAGGCAAGCA 3901 AAATGCCGCA AAGAAGGGAA TGAGTGCGAC ACGAAAATGT TGGATGCTCA TACTCGTCCT 3961 TTTTCAATAT TATTGAAGCA TTTATCAGGG TTACTAGTAC GTCTCTCAAG GATAAGTAAG 4021 TAATATTAAG GTACGGGAGG TATTGGACAG GCCGCAATAA AATATCTTTA TTTTCATTAC 4081 ATCTGTGTGT TGGTTTTTTG TGTGAATCGA TAGTACTAAC ATACGCTCTC CATCAAAACA 4141 AAACGAAACA AAACAAACTA GCAAAATAGG CTGTCCCCAG TGCAAGTGCA GGTGCCAGAA 4201 CATTTCTCT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.