pCMV-EGFP-PRICKLE2-3×FLAG-Neo

pCMV-EGFP-PRICKLE2-3×FLAG-Neo plasmid

PVT94720

pCMV-EGFP-PRICKLE2-3×FLAG-Neo plasmid Information

Plasmid name: pCMV-EGFP-PRICKLE2(human)-3×FLAG-Neo
Alias: NM_198859.4;EPM5
Gene lenght: 2532bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pCMV-EGFP-PRICKLE2-3×FLAG-Neo plasmid Description

pCMV-EGFP-PRICKLE2-3×FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFP-PRICKLE2-3×FLAG-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaacgg gccctctaga ctcgagcggc cgccactgtg ctggatatct gcagaattcG 961 CCACCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC CTGGTCGAGC 1021 TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG GGCGATGCCA 1081 CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC GTGCCCTGGC 1141 CCACCCTCGT GACCACCCTG ACCTACGGCG TGCAGTGCTT CAGCCGCTAC CCCGACCACA 1201 TGAAGCAGCA CGACTTCTTC AAGTCCGCCA TGCCCGAAGG CTACGTCCAG GAGCGCACCA 1261 TCTTCTTCAA GGACGACGGC AACTACAAGA CCCGCGCCGA GGTGAAGTTC GAGGGCGACA 1321 CCCTGGTGAA CCGCATCGAG CTGAAGGGCA TCGACTTCAA GGAGGACGGC AACATCCTGG 1381 GGCACAAGCT GGAGTACAAC TACAACAGCC ACAACGTCTA TATCATGGCC GACAAGCAGA 1441 AGAACGGCAT CAAGGTGAAC TTCAAGATCC GCCACAACAT CGAGGACGGC AGCGTGCAGC 1501 TCGCCGACCA CTACCAGCAG AACACCCCCA TCGGCGACGG CCCCGTGCTG CTGCCCGACA 1561 ACCACTACCT GAGCACCCAG TCCGCCCTGA GCAAAGACCC CAACGAGAAG CGCGATCACA 1621 TGGTCCTGCT GGAGTTCGTG ACCGCCGCCG GGATCACTCT CGGCATGGAC GAGCTGTACA 1681 AGggtggagg cggatcagga ggcggtggat ctatggtgac agtgatgccg ctggagatgg 1741 agaagaccat cagcaaactc atgtttgact ttcagaggaa ctcgacctca gatgatgact 1801 caggctgtgc tttggaagag tatgcctggg tcccgccggg tctgaagcct gaacaggtac 1861 accagtacta tagctgtctc ccagaagaga aagtccctta tgtcaacagt cctggagaga 1921 aactgcgaat caagcagcta ctacaccagc tgccgccaca tgacaatgag gttcgatatt 1981 gcaactccct ggatgaggaa gagaagaggg agctgaagct tttcagcagc cagaggaaac 2041 gcgaaaactt gggccgcggg aatgtcaggc ctttcccagt caccatgaca ggagctattt 2101 gtgaacagtg cggaggccag atcaatggtg gagacatcgc tgtgtttgcg tcacgcgctg 2161 gccacggcgt ttgctggcac ccgccgtgct tcgtatgcac tgtctgcaat gagctcctgg 2221 tggatctgat ctacttttac caagatggga agatatactg tggcaggcac catgctgagt 2281 gcctgaagcc gcgctgtgct gcctgcgatg agatcatctt tgcagatgaa tgcacagaag 2341 ctgaggggcg acactggcac atgaaacact tttgctgctt cgagtgtgag acagtgctgg 2401 gcggccagcg ctacatcatg aaggagggaa gaccctactg ttgccactgc ttcgagtcct 2461 tgtatgcaga atattgtgac acctgtgccc aacatatagg tatcgaccaa ggtcaaatga 2521 cctatgatgg ccaacactgg catgccactg agacctgttt ctgctgtgct cactgcaaga 2581 aatccctcct ggggcggcca ttcctcccga agcagggcca gatattctgc tcacgggcct 2641 gcagtgctgg ggaagacccc aatggttctg actcctctga ttccgccttc cagaacgcca 2701 gggccaagga gtcccggcgc agtgccaaaa ttggcaagaa caagggcaag acggaggagc 2761 ccatgctgaa ccagcacagc cagctgcaag tgagttctaa ccggctgtca gccgacgtag 2821 accccctgtc actgcagatg gacatgctca gcctgtccag ccagacaccc agcctcaacc 2881 gggaccccat ctggaggagc cgggaagagc cctaccatta tgggaacaag atggagcaga 2941 accagaccca gagccctctg cagctcctca gccagtgcaa catcagaact tcctacagtc 3001 caggagggca aggggctggg gcccagcccg aaatgtgggg caagcacttc agcaacccca 3061 aaaggagctc gtcactggcc atgacaggac atgctggcag cttcatcaag gaatgccgag 3121 aagactatta cccggggagg ctgagatctc aggagagcta cagtgatatg tctagtcaga 3181 gtttcagtga gacccgaggc agcatccaag tccccaaata tgaggaggaa gaggaagagg 3241 aagggggctt gtccactcag cagtgtcgga cccgtcatcc catcagttcc ctgaaataca 3301 cagaggacat gacgcccaca gagcagaccc ctcggggctc catggaatcc ctggccctgt 3361 ctaatgcaac aggcctctct gctgatggtg gtgccaagcg ccaggagcac ctatcccgat 3421 tttccatgcc tgacctcagc aaagactctg gaatgaatgt gtctgagaag ctgagcaaca 3481 tgggcactct taactcgtcc atgcagttcc ggagcgcaga gtcagttcgc agcctgctct 3541 ctgcccagca gtaccaggag atggagggaa acctccacca gctcagcaac cccattggct 3601 acagagacct gcagtcccac ggaaggatgc atcagagctt tgattttgat ggagggatgg 3661 cgggcagcaa gctgccaggg caggagggcg tgaggatcca gcccatgagt gaacgcaccc 3721 ggagaagagc tacttcacgc gacgacaacc gccgtttccg acctcacagg tccaggcgtt 3781 cccgacgctc tcgctccgac aacgccctcc acctggccag cgaacgcgag gccatctccc 3841 ggttaaaaga taggccccct ctgagagcca gggaggacta tgaccaattt atgcgccagc 3901 ggagcttcca ggagagcatg gggcatgggt cccggaggga cctgtacggc cagtgcccta 3961 ggactgtgtc ggacctggct ttgcagaatg cctttgggga ccgctgggga ccctacttcg 4021 ccgagtatga ttggtgttcc acctgctcct cctcttcaga gtctgacaac gagggctatt 4081 tcctaggaga acccatcccc cagccagcgc gcctgcgata cgtcacaagc gatgagctgc 4141 tgcacaaata cagctcctac ggcctcccca aatcttccac attaggtggc agaggacagt 4201 tgcacagcag gaaaagacag aagagcaaaa actgtatcat ttctGACTAC AAAGACCATG 4261 ACGGTGATTA TAAAGATCAT GACATCGATT ACAAGGATGA CGATGACAAG tagcttaagt 4321 ttaaaccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc 4381 tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat 4441 gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg 4501 caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc 4561 tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc ccacgcgccc 4621 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt 4681 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc 4741 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta 4801 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc 4861 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg 4921 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt 4981 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat 5041 taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca 5101 gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct 5161 ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc 5221 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg 5281 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc 5341 agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt 5401 gtatatccat tttcggatct gatcaagaga caggatgagg atcgtttcgc atgattgaac 5461 aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc ggctatgact 5521 gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca gcgcaggggc 5581 gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg caggacgagg 5641 cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg ctcgacgttg 5701 tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag gatctcctgt 5761 catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg cggcggctgc 5821 atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc atcgagcgag 5881 cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa gagcatcagg 5941 ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac ggcgaggatc 6001 tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat ggccgctttt 6061 ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac atagcgttgg 6121 ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc ctcgtgcttt 6181 acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt gacgagttct 6241 tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc tgccatcacg 6301 agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga 6361 cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa 6421 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa 6481 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta 6541 tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt 6601 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa 6661 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact 6721 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc 6781 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg 6841 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc 6901 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag 6961 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca 7021 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca 7081 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg 7141 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag 7201 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt 7261 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca 7321 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg 7381 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt 7441 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc 7501 cggcaaacaa accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag 7561 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 7621 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 7681 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 7741 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 7801 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 7861 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 7921 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 7981 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 8041 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 8101 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 8161 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 8221 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 8281 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 8341 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 8401 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 8461 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 8521 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 8581 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 8641 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 8701 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.