pLV3-CMV-ETS2-3×FLAG-EF1a-CopG

pLV3-CMV-ETS2-3×FLAG-EF1a-CopGFP-Neo plasmid

PVT94711

pLV3-CMV-ETS2-3×FLAG-EF1a-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-ETS2(human)-3×FLAG-EF1a-CopGFP-Neo
Alias: NM_005239.6;ETS2IT1
Gene lenght: 1407bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ETS2-3×FLAG-EF1a-CopGFP-Neo plasmid Description

pLV3-CMV-ETS2-3×FLAG-EF1a-CopGFP-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-ETS2-3×FLAG-EF1a-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgaatga tttcggaatc aagaatatgg accaggtagc 2341 ccctgtggct aacagttaca gagggacact caagcgccag ccagcctttg acacctttga 2401 tgggtccctg tttgctgttt ttccttctct aaatgaagag caaacactgc aagaagtgcc 2461 aacaggcttg gattccattt ctcatgactc cgccaactgt gaattgcctt tgttaacccc 2521 gtgcagcaag gctgtgatga gtcaagcctt aaaagctacc ttcagtggct tcaaaaagga 2581 acagcggcgc ctgggcattc caaagaaccc ctggctgtgg agtgagcaac aggtatgcca 2641 gtggcttctc tgggccacca atgagttcag tctggtgaac gtgaatctgc agaggttcgg 2701 catgaatggc cagatgctgt gtaaccttgg caaggaacgc tttctggagc tggcacctga 2761 ctttgtgggt gacattctct gggaacatct ggagcaaatg atcaaagaaa accaagaaaa 2821 gacagaagat caatatgaag aaaattcaca cctcacctcc gttcctcatt ggattaacag 2881 caatacatta ggttttggca cagagcaggc gccctatgga atgcagacac agaattaccc 2941 caaaggcggc ctcctggaca gcatgtgtcc ggcctccaca cccagcgtac tcagctctga 3001 gcaggagttt cagatgttcc ccaagtctcg gctcagctcc gtcagcgtca cctactgctc 3061 tgtcagtcag gacttcccag gcagcaactt gaatttgctc accaacaatt ctgggactcc 3121 caaagaccac gactcccctg agaacggtgc ggacagcttc gagagctcag actccctcct 3181 ccagtcctgg aacagccagt cgtccttgct ggatgtgcaa cgggttcctt ccttcgagag 3241 cttcgaagat gactgcagcc agtctctctg cctcaataag ccaaccatgt ctttcaagga 3301 ttacatccaa gagaggagtg acccagtgga gcaaggcaaa ccagttatac ctgcagctgt 3361 gctggccggc ttcacaggaa gtggacctat tcagctgtgg cagtttctcc tggagctgct 3421 atcagacaaa tcctgccagt cattcatcag ctggactgga gacggatggg agtttaagct 3481 cgccgacccc gatgaggtgg cccgccggtg gggaaagagg aaaaataagc ccaagatgaa 3541 ctacgagaag ctgagccggg gcttacgcta ctattacgac aagaacatca tccacaagac 3601 gtcggggaag cgctacgtgt accgcttcgt gtgcgacctc cagaacttgc tggggttcac 3661 gcccgaggaa ctgcacgcca tcctgggcgt ccagcccgac acggaggacg gatccGACTA 3721 CAAAGACCAT GACGGTGATT ATAAAGATCA TGACATCGAC TACAAGGATG ACGATGACAA 3781 GTAgcggccg cGaaggatct gcgatcgctc cggtgcccgt cagtgggcag agcgcacatc 3841 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg cctagagaag 3901 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg 3961 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt 4021 tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac gcgcccgccg 4081 ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc ccgcctgtgg 4141 tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga gaccgggcct 4201 ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc tttgcctgac 4261 cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt acagatccaa 4321 gctgtgaccg gcgcctacgc tagacgccac catggagagc gacgagagcg gcctgcccgc 4381 catggagatc gagtgccgca tcaccggcac cctgaacggc gtggagttcg agctggtggg 4441 cggcggagag ggcaccccca agcagggccg catgaccaac aagatgaaga gcaccaaagg 4501 cgccctgacc ttcagcccct acctgctgag ccacgtgatg ggctacggct tctaccactt 4561 cggcacctac cccagcggct acgagaaccc cttcctgcac gccatcaaca acggcggcta 4621 caccaacacc cgcatcgaga agtacgagga cggcggcgtg ctgcacgtga gcttcagcta 4681 ccgctacgag gccggccgcg tgatcggcga cttcaaggtg gtgggcaccg gcttccccga 4741 ggacagcgtg atcttcaccg acaagatcat ccgcagcaac gccaccgtgg agcacctgca 4801 ccccatgggc gataacgtgc tggtgggcag cttcgcccgc accttcagcc tgcgcgacgg 4861 cggctactac agcttcgtgg tggacagcca catgcacttc aagagcgcca tccaccccag 4921 catcctgcag aacgggggcc ccatgttcgc cttccgccgc gtggaggagc tgcacagcaa 4981 caccgagctg ggcatcgtgg agtaccagca cgccttcaag acccccatcg ccttcgccag 5041 atcccgcgct cagtcgtcca attctgccgt ggacggcacc gccggacccg gctccaccgg 5101 atctcgcgag ggcagaggaa gtcttctaac atgcggtgac gtggaggaga atcccggccc 5161 tGCCACCatg gctgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag 5221 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg 5281 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa 5341 tgaactgcag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc 5401 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc 5461 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga 5521 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa 5581 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct 5641 ggacgaagag catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat 5701 gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt 5761 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta 5821 tcaggacata gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga 5881 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg 5941 ccttcttgac gagttcttct gaGTCGACAA TCAACCTCTG GATTACAAAA TTTGTGAAAG 6001 ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT 6061 GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC 6121 CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG 6181 CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT 6241 TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT 6301 TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG 6361 GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC 6421 GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT 6481 GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT 6541 TTGGGCCGCC TCCCCGCggt acctttaaga ccaatgactt acaaggcagc tgtagatctt 6601 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaaGataa 6661 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 6721 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 6781 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 6841 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 6901 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt 6961 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 7021 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc 7081 ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt 7141 atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt 7201 ttggaggcct agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc 7261 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 7321 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 7381 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 7441 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 7501 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 7561 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 7621 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 7681 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 7741 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 7801 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 7861 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 7921 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 7981 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 8041 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 8101 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 8161 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 8221 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 8281 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 8341 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 8401 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 8461 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 8521 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 8581 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 8641 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 8701 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 8761 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 8821 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 8881 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 8941 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 9001 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 9061 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 9121 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 9181 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 9241 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 9301 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 9361 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 9421 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg 9481 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 9541 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 9601 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt 9661 gtgaaatacc gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca 9721 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg 9781 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac 9841 gacgttgtaa aacgacggcc agtgccaagc tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.