pCMV-GRM1-FLAG-TwinStrep-Neo p

pCMV-GRM1-FLAG-TwinStrep-Neo plasmid

PVT93532

pCMV-GRM1-FLAG-TwinStrep-Neo plasmid Information

Plasmid name: pCMV-GRM1(human)-FLAG-TwinStrep-Neo
Alias: NM_001278064.2;GPRC1A; MGLU1; MGLUR1; PPP1R85; SCA44;SCAR13
Gene lenght: 3582bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-GRM1-FLAG-TwinStrep-Neo plasmid Description

pCMV-GRM1-FLAG-TwinStrep-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-GRM1-FLAG-TwinStrep-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTGAATT CGCCACCatg gtcgggctcc ttttgttttt tttcccagcg 961 atctttttgg aggtgtccct tctccccaga agccccggca ggaaagtgtt gctggcagga 1021 gcgtcgtctc agcgctcggt ggccagaatg gacggagatg tcatcattgg agccctcttc 1081 tcagtccatc accagcctcc ggccgagaaa gtgcccgaga ggaagtgtgg ggagatcagg 1141 gagcagtatg gcatccagag ggtggaggcc atgttccaca cgttggataa gatcaacgcg 1201 gacccggtcc tcctgcccaa catcaccctg ggcagtgaga tccgggactc ctgctggcac 1261 tcttccgtgg ctctggaaca gagcattgag ttcattaggg actctctgat ttccattcga 1321 gatgagaagg atgggatcaa ccggtgtctg cctgacggcc agtccctccc cccaggcagg 1381 actaagaagc ccattgcggg agtgatcggt cccggctcca gctctgtagc cattcaagtg 1441 cagaacctgc tccagctctt cgacatcccc cagatcgctt attcagccac aagcatcgac 1501 ctgagtgaca aaactttgta caaatacttc ctgagggttg tcccttctga cactttgcag 1561 gcaagggcca tgcttgacat agtcaaacgt tacaattgga cctatgtctc tgcagtccac 1621 acggaaggga attatgggga gagcggaatg gacgctttca aagagctggc tgcccaggaa 1681 ggcctctgta tcgcccattc tgacaaaatc tacagcaacg ctggggagaa gagctttgac 1741 cgactcttgc gcaaactccg agagaggctt cccaaggcta gagtggtggt ctgcttctgt 1801 gaaggcatga cagtgcgagg actcctgagc gccatgcggc gccttggcgt cgtgggcgag 1861 ttctcactca ttggaagtga tggatgggca gacagagatg aagtcattga aggttatgag 1921 gtggaagcca acgggggaat cacgataaag ctgcagtctc cagaggtcag gtcatttgat 1981 gattatttcc tgaaactgag gctggacact aacacgagga atccctggtt ccctgagttc 2041 tggcaacatc ggttccagtg ccgccttcca ggacaccttc tggaaaatcc caactttaaa 2101 cgaatctgca caggcaatga aagcttagaa gaaaactatg tccaggacag taagatgggg 2161 tttgtcatca atgccatcta tgccatggca catgggctgc agaacatgca ccatgccctc 2221 tgccctggcc acgtgggcct ctgcgatgcc atgaagccca tcgacggcag caagctgctg 2281 gacttcctca tcaagtcctc attcattgga gtatctggag aggaggtgtg gtttgatgag 2341 aaaggagacg ctcctggaag gtatgatatc atgaatctgc agtacactga agctaatcgc 2401 tatgactatg tgcacgttgg aacctggcat gaaggagtgc tgaacattga tgattacaaa 2461 atccagatga acaagagtgg agtggtgcgg tctgtgtgca gtgagccttg cttaaagggc 2521 cagattaagg ttatacggaa aggagaagtg agctgctgct ggatttgcac ggcctgcaaa 2581 gagaatgaat atgtgcaaga tgagttcacc tgcaaagctt gtgacttggg atggtggccc 2641 aatgcagatc taacaggctg tgagcccatt cctgtgcgct atcttgagtg gagcaacatc 2701 gaatccatta tagccatcgc cttttcatgc ctgggaatcc ttgttacctt gtttgtcacc 2761 ctaatctttg tactgtaccg ggacacacca gtggtcaaat cctccagtcg ggagctctgc 2821 tacatcatcc tagctggcat cttccttggt tatgtgtgcc cattcactct cattgccaaa 2881 cctactacca cctcctgcta cctccagcgc ctcttggttg gcctctcctc tgcgatgtgc 2941 tactctgctt tagtgactaa aaccaatcgt attgcacgca tcctggctgg cagcaagaag 3001 aagatctgca cccggaagcc caggttcatg agtgcctggg ctcaggtgat cattgcctca 3061 attctgatta gtgtgcaact aaccctggtg gtaaccctga tcatcatgga accccctatg 3121 cccattctgt cctacccaag tatcaaggaa gtctacctta tctgcaatac cagcaacctg 3181 ggtgtggtgg cccctttggg ctacaatgga ctcctcatca tgagctgtac ctactatgcc 3241 ttcaagaccc gcaacgtgcc cgccaacttc aacgaggcca aatatatcgc gttcaccatg 3301 tacaccacct gtatcatctg gctagctttt gtgcccattt actttgggag caactacaag 3361 atcatcacaa cttgctttgc agtgagtctc agtgtaacag tggctctggg gtgcatgttc 3421 actcccaaga tgtacatcat tattgccaag cctgagagga atgtccgcag tgccttcacc 3481 acctctgatg ttgtccgcat gcatgttggc gatggcaagc tgccctgccg ctccaacact 3541 ttcctcaaca tcttccgaag aaagaaggca ggggcaggga atgccaattc taatggcaag 3601 tctgtgtcat ggtctgaacc aggtggagga caggtgccca agggacagca tatgtggcac 3661 cgcctctctg tgcacgtgaa gaccaatgag acggcctgca accaaacagc cgtcatcaag 3721 cccctcacta aaagttacca aggctctggc aagagcctga ccttttcaga taccagcacc 3781 aagacccttt acaacgtaga ggaggaggag gatgcccagc cgattcgctt tagcccgcct 3841 ggtagccctt ccatggtggt gcacaggcgc gtgccaagcg cggcgaccac tccgcctctg 3901 ccgtcccacc tgaccgcaga ggagaccccc ctcttcctgg ccgaaccagc cctccccaag 3961 ggcttgcccc ctcctctcca gcagcagcag caaccccctc cacagcagaa atcgctgatg 4021 gaccagctcc agggagtggt cagcaacttc agtaccgcga tcccggattt tcacgcggtg 4081 ctggcaggcc ccggtggtcc cgggaacggg ctgcggtccc tgtacccgcc cccgccacct 4141 ccgcagcacc tgcagatgct gccgctgcag ctgagcacct ttggggagga gctggtctcc 4201 ccgcccgcgg acgacgacga cgacagcgag aggtttaagc tcctccagga gtacgtgtat 4261 gagcacgagc gggaagggaa cacggaagaa gacgaactgg aagaggagga ggaggacctg 4321 caggcggcca gcaaactgac cccggatgat tcgcctgcgc tgacgcctcc gtcgcctttc 4381 cgcgactcgg tggcctcggg cagctcggtg cccagctccc ccgtgtccga gtcggtgctc 4441 tgcacccctc ccaacgtatc ctacgcctct gtcattctgc gggactacaa gcaaagctct 4501 tccaccctgC TCGAGGATTA CAAGGATGAC GACGATAAGG GATCCagcgc ctggtcccac 4561 cctcagtttg agaagggcgg aggctctggc ggcggaagcg gaggatctgc ttggagccac 4621 ccccagttcg aaaagtgaTG AGGGCCCGTT TAAACCCGCT GATCAGCCTC GACTGTGCCT 4681 TCTAGTTGCC AGCCATCTGT TGTTTGCCCC TCCCCCGTGC CTTCCTTGAC CCTGGAAGGT 4741 GCCACTCCCA CTGTCCTTTC CTAATAAAAT GAGGAAATTG CATCGCATTG TCTGAGTAGG 4801 TGTCATTCTA TTCTGGGGGG TGGGGTGGGG CAGGACAGCA AGGGGGAGGA TTGGGAAGAC 4861 AATAGCAGGC ATGCTGGGGA TGCGGTGGGC TCTATGGCTT CTGAGGCGGA AAGAACCAGC 4921 TGGGGCTCTA GGGGGTATCC CCACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 4981 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 5041 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 5101 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 5161 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 5221 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 5281 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 5341 GAGCTGATTT AACAAAAATT TAACGCGAAT TAATTCTGTG GAATGTGTGT CAGTTAGGGT 5401 GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT 5461 CAGCAACCAG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC 5521 ATCTCAATTA GTCAGCAACC ATAGTCCCGC CCCTAACTCC GCCCATCCCG CCCCTAACTC 5581 CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT TTTTTTTATT TATGCAGAGG 5641 CCGAGGCCGC CTCTGCCTCT GAGCTATTCC AGAAGTAGTG AGGAGGCTTT TTTGGAGGCC 5701 TAGGCTTTTG CAAAAAGCTC CCGGGAGCTT GTATATCCAT TTTCGGATCT GATCAAGAGA 5761 CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT GCACGCAGGT TCTCCGGCCG 5821 CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA GACAATCGGC TGCTCTGATG 5881 CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT TTTTGTCAAG ACCGACCTGT 5941 CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT ATCGTGGCTG GCCACGACGG 6001 GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC GGGAAGGGAC TGGCTGCTAT 6061 TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT TGCTCCTGCC GAGAAAGTAT 6121 CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA TCCGGCTACC TGCCCATTCG 6181 ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG GATGGAAGCC GGTCTTGTCG 6241 ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC AGCCGAACTG TTCGCCAGGC 6301 TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC CCATGGCGAT GCCTGCTTGC 6361 CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT CGACTGTGGC CGGCTGGGTG 6421 TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA TATTGCTGAA GAGCTTGGCG 6481 GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC CGCTCCCGAT TCGCAGCGCA 6541 TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG ACTCTGGGGT TCGAAATGAC 6601 CGACCAAGCG ACGCCCAACC TGCCATCACG AGATTTCGAT TCCACCGCCG CCTTCTATGA 6661 AAGGTTGGGC TTCGGAATCG TTTTCCGGGA CGCCGGCTGG ATGATCCTCC AGCGCGGGGA 6721 TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTTGTTTATT GCAGCTTATA ATGGTTACAA 6781 ATAAAGCAAT AGCATCACAA ATTTCACAAA TAAAGCATTT TTTTCACTGC ATTCTAGTTG 6841 TGGTTTGTCC AAACTCATCA ATGTATCTTA TCATGTCTGT ATACCGTCGA CCTCTAGCTA 6901 GAGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA AATTGTTATC CGCTCACAAT 6961 TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC TGGGGTGCCT AATGAGTGAG 7021 CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC CAGTCGGGAA ACCTGTCGTG 7081 CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC GGTTTGCGTA TTGGGCGCTC 7141 TTCCGCTTCC TCGCTCACTG ACTCGCTGCG CTCGGTCGTT CGGCTGCGGC GAGCGGTATC 7201 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 7261 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 7321 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 7381 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 7441 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 7501 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 7561 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 7621 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 7681 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 7741 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 7801 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTTT 7861 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 7921 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 7981 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 8041 AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC 8101 ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA 8161 GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA 8221 CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG 8281 CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC 8341 TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT 8401 CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG 8461 GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT 8521 CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA 8581 TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA 8641 GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA 8701 TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG 8761 GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC 8821 ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG 8881 AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT 8941 CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT 9001 ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT 9061 GCCACCTGAC GTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.