pLV3-CMV-HLX-3×FLAG-EF1a-CopGF

pLV3-CMV-HLX-3×FLAG-EF1a-CopGFP-Neo plasmid

PVT94701

pLV3-CMV-HLX-3×FLAG-EF1a-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-HLX(human)-3×FLAG-EF1a-CopGFP-Neo
Alias: NM_021958.4;HB24; HLX1
Gene lenght: 1464bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-HLX-3×FLAG-EF1a-CopGFP-Neo plasmid Description

pLV3-CMV-HLX-3×FLAG-EF1a-CopGFP-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-HLX-3×FLAG-EF1a-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatgttcgc agccgggctg gctcccttct acgcctccaa 2341 cttcagcctc tggtcggccg cttactgctc ctcggccggc ccaggcggct gctccttccc 2401 cttggacccc gccgccgtca aaaagccctc cttctgcatc gcagacattc tgcacgccgg 2461 cgtgggggat ctgggggcgg ccccggaggg cctggcaggg gcctcggccg ccgccctcac 2521 cgcgcacttg ggctcggttc acccgcacgc ctctttccaa gcggcggcca gatccccgct 2581 tcgacccacc ccagtggtgg cgccctccga agtcccggct ggcttcccgc agcggctgtc 2641 tccgctctca gccgcctacc accaccatca cccgcaacaa caacagcagc agcaacagcc 2701 gcagcagcaa cagcctccgc ctccgccccg ggctggcgcc ctgcagcccc cggcctcggg 2761 gacgcgagtg gttccgaacc cccaccacag tggctctgcc ccggccccct ccagcaaaga 2821 cctcaaattt ggaattgacc gcattttatc tgcagaattt gacccaaaag tcaaagaagg 2881 caacacgctg agagatctca cttccctgct aaccggtggg cggcccgccg gggtgcacct 2941 ctcaggcctg cagccctcgg ccggccagtt cttcgcatct ctagatccca ttaacgaggc 3001 ttctgcaatc ctgagtccct taaactcgaa cccaagaaat tcagttcagc atcagttcca 3061 agacacgttt ccaggtccct atgctgtgct cacgaaggac accatgccgc agacgtacaa 3121 aaggaagcgt tcatggtcgc gcgctgtgtt ctccaacctg cagaggaaag gcctggagaa 3181 aaggtttgag attcagaagt acgtgaccaa gccggaccga aagcagctgg cggcgatgct 3241 gggcctcacg gacgcacagg tgaaggtgtg gttccagaac cggcggatga agtggcggca 3301 ctccaaggag gcccaggccc aaaaggacaa ggacaaggag gctggcgaga agccatcagg 3361 tggagccccg gctgcggatg gcgagcagga cgagaggagc cccagccgtt ctgaaggcga 3421 ggctgagagc gagagcagcg actccgagtc cctggacatg gcccccagcg acacggagcg 3481 gactgagggg agtgagcgtt ctctgcacca aacaacagtt attaaggccc cggtcactgg 3541 cgccctcatt accgccagca gtgctgggag tggtgggagc agcggcggcg gcggcaatag 3601 tttcagcttc agcagcgcca gcagtcttag tagcagcagc accagtgcgg gttgcgccag 3661 cagccttggc ggcggcggcg cctcggagct tctccctgca acacagccca cagccagcag 3721 cgctcccaaa agccccgagc cagcccaagg cgcgcttggc tgcttaggat ccGACTACAA 3781 AGACCATGAC GGTGATTATA AAGATCATGA CATCGACTAC AAGGATGACG ATGACAAGTA 3841 gcggccgcGa aggatctgcg atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc 3901 cacagtcccc gagaagttgg ggggaggggt cggcaattga acgggtgcct agagaaggtg 3961 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg 4021 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc 4081 cgccagaaca cagctgaagc ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc 4141 tacctgaggc cgccatccac gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc 4201 ctcctgaact gcgtccgccg tctaggtaag tttaaagctc aggtcgagac cgggcctttg 4261 tccggcgctc ccttggagcc tacctagact cagccggctc tccacgcttt gcctgaccct 4321 gcttgctcaa ctctacgtct ttgtttcgtt ttctgttctg cgccgttaca gatccaagct 4381 gtgaccggcg cctacgctag acgccaccat ggagagcgac gagagcggcc tgcccgccat 4441 ggagatcgag tgccgcatca ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg 4501 cggagagggc acccccaagc agggccgcat gaccaacaag atgaagagca ccaaaggcgc 4561 cctgaccttc agcccctacc tgctgagcca cgtgatgggc tacggcttct accacttcgg 4621 cacctacccc agcggctacg agaacccctt cctgcacgcc atcaacaacg gcggctacac 4681 caacacccgc atcgagaagt acgaggacgg cggcgtgctg cacgtgagct tcagctaccg 4741 ctacgaggcc ggccgcgtga tcggcgactt caaggtggtg ggcaccggct tccccgagga 4801 cagcgtgatc ttcaccgaca agatcatccg cagcaacgcc accgtggagc acctgcaccc 4861 catgggcgat aacgtgctgg tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg 4921 ctactacagc ttcgtggtgg acagccacat gcacttcaag agcgccatcc accccagcat 4981 cctgcagaac gggggcccca tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac 5041 cgagctgggc atcgtggagt accagcacgc cttcaagacc cccatcgcct tcgccagatc 5101 ccgcgctcag tcgtccaatt ctgccgtgga cggcaccgcc ggacccggct ccaccggatc 5161 tcgcgagggc agaggaagtc ttctaacatg cggtgacgtg gaggagaatc ccggccctGC 5221 CACCatggct gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct 5281 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct 5341 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga 5401 actgcaggac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc 5461 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg 5521 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc 5581 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca 5641 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga 5701 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc 5761 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga 5821 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca 5881 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg 5941 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct 6001 tcttgacgag ttcttctgaG TCGACAATCA ACCTCTGGAT TACAAAATTT GTGAAAGATT 6061 GACTGGTATT CTTAACTATG TTGCTCCTTT TACGCTATGT GGATACGCTG CTTTAATGCC 6121 TTTGTATCAT GCTATTGCTT CCCGTATGGC TTTCATTTTC TCCTCCTTGT ATAAATCCTG 6181 GTTGCTGTCT CTTTATGAGG AGTTGTGGCC CGTTGTCAGG CAACGTGGCG TGGTGTGCAC 6241 TGTGTTTGCT GACGCAACCC CCACTGGTTG GGGCATTGCC ACCACCTGTC AGCTCCTTTC 6301 CGGGACTTTC GCTTTCCCCC TCCCTATTGC CACGGCGGAA CTCATCGCCG CCTGCCTTGC 6361 CCGCTGCTGG ACAGGGGCTC GGCTGTTGGG CACTGACAAT TCCGTGGTGT TGTCGGGGAA 6421 ATCATCGTCC TTTCCTTGGC TGCTCGCCTG TGTTGCCACC TGGATTCTGC GCGGGACGTC 6481 CTTCTGCTAC GTCCCTTCGG CCCTCAATCC AGCGGACCTT CCTTCCCGCG GCCTGCTGCC 6541 GGCTCTGCGG CCTCTTCCGC GTCTTCGCCT TCGCCCTCAG ACGAGTCGGA TCTCCCTTTG 6601 GGCCGCCTCC CCGCggtacc tttaagacca atgacttaca aggcagctgt agatcttagc 6661 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aaGataagat 6721 ctgctttttg cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct 6781 ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta 6841 gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca 6901 gtgtggaaaa tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt 6961 gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac 7021 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 7081 tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc 7141 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 7201 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 7261 gaggcctaga cttttgcaga gacggcccaa attcgtaatc atggtcatag ctgtttcctg 7321 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 7381 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 7441 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 7501 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 7561 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 7621 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 7681 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 7741 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 7801 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 7861 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 7921 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 7981 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 8041 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 8101 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 8161 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 8221 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 8281 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 8341 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 8401 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 8461 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 8521 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 8581 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 8641 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 8701 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 8761 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 8821 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 8881 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 8941 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 9001 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 9061 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 9121 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 9181 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 9241 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 9301 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 9361 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 9421 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 9481 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg 9541 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 9601 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 9661 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg 9721 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgccattcg ccattcaggc 9781 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga 9841 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac 9901 gttgtaaaac gacggccagt gccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.