pLV3-CMV-Trps1-3×FLAG-CopGFP-P

pLV3-CMV-Trps1-3×FLAG-CopGFP-Puro-WPRE plasmid

PVT94669

pLV3-CMV-Trps1-3×FLAG-CopGFP-Puro-WPRE plasmid Information

Plasmid name: pLV3-CMV-Trps1(mouse)-3×FLAG-CopGFP-Puro-WPRE
Alias: NM_032000.2;D15Ertd586e
Gene lenght: 3843bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pLV3-CMV-Trps1-3×FLAG-CopGFP-Puro-WPRE plasmid Description

pLV3-CMV-Trps1-3×FLAG-CopGFP-Puro-WPRE is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Trps1-3×FLAG-CopGFP-Puro-WPRE plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtttatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 tagcgaattc GCCACCatgg tccggaaaaa gaacccccct ctgagaaacg ttgctagtga 2341 aggcgagggt caaaccctgg agccaacagc tacagagagc aaggtatctg ggaagaacaa 2401 agaattgtct gcagatcaga tgtcagaaaa tacagatcag agtgatgttg cagagttgaa 2461 ctcgaaggag gaacacagca cgcatggcca agagccatct tccagcggta agaaggactt 2521 gcaaatctca ggcctgagtg agaaggctgg cttcaattat gaaagcccca gtaagggagg 2581 gagccttgtc tccttcccac atgatgaggt aacagacaga aatatgttgg ctttctcatc 2641 tccagctgct gggggagtct gcgagccctt gaagtctcct caaagagcag aggcagatga 2701 ccctcaagat atggcctgca ccccatcagg ggattcattg gagacaaagg aagagcataa 2761 aatgtcacca aaggccactg aggaaacagg gccagtgcag agtggtcaag ccaattgtca 2821 aggtttgagt ccagtttccg tggcctcaaa aaacccacaa gtgccttcag atgggggtgt 2881 cagactgagt aaacccaaag gtgacttact ggtgaatgac aacccagacc cggcacctct 2941 gtctccagag cttcaggact ttaaatgcaa tatctgtgga tacggttact atggcaatga 3001 ccccacagat ctgattaagc acttccgaaa gtatcactta ggactgcata atcgcaccag 3061 gcaggatgct gagctggaca gcaaaatctt ggcccttcat aacatggtgc agttcagcca 3121 ttccaaagac ttccagaagg tcaaccgttc tgtgctttct ggcgtgctgc aggatatcag 3181 ctcttcacgg cctgctttac taaacggaac ctatgatgta caggtcactt caggaggaac 3241 tttcattggc attggacgga aaactccaga ttgccaaggc aacaccaagt atttccgctg 3301 caaattctgc aatttcactt atatgggaaa ctcctcaact gaactagagc aacattttct 3361 tcagacgcac ccgaataaaa tcaaagtgtc tctcccgtcc tccgagggtg tgaaaccttc 3421 agagaaaaac tctaacaaat ccatccctgc gcttcgagcc agtgactctg gggatgtggg 3481 aaagtggcag gacaaaatga cagtcaaagc tggagatgac acccctgtcg gctactcagt 3541 gcccatcaag cccctcgatt cctcacgaca aaatggtaca gaggccacca gttattactg 3601 gtgtaaattt tgtagtttca gctgtgagtc atccagctcc cttaagctgc tagaacatta 3661 tggcaagcag cacggagcag tgcagtcggg cggccttaat ccagagttga atgacaagct 3721 tcccaggggc tctgtcatta atcagaatga tctagccaaa agtgtagaag gagagccatt 3781 gaccaagcca gaaaagggct tgagtggggc taaaaagaag gacttcccca gcaagggagc 3841 agaggataat atggtaacga gctataactg tcagttctgt gactttagat attccaaaag 3901 ccatggccct gatgtgattg tagtgggtcc acttctccgt cattatcagc aactccataa 3961 catccacaag tgtaccatta agcactgtcc attctgtccc agaggccttt gcagcccaga 4021 aaagcacctt ggagagatta cttatccgtt tgcttgtaga aaaagtaatt gttcccactg 4081 tgcactgttg ctgttgcact tgtctcctgg ggtggccgga agttcgagag tcaaacacca 4141 atgccaccag tgttccttct ctacccccga tgtagatgtg ctcctctttc actatgagac 4201 cgtgcatgag tcccaagcat cggatgtcaa acaggaggcc aaccacctgc taggatctga 4261 cgggcagcag gctgtcaggg acagcaagga gcactcgtgc accaaatgcg attttataac 4321 ccaggtggaa gaagagatct cgagacacta caggagagca cacagctgct ataaatgtcg 4381 tcagtgcagt ttcacagctg ctgacactca gtcactattg gagcacttca acacagttca 4441 ctgccaggaa caggaaatca ctactgctaa tggcgaagag ggtggccatg ccatacccac 4501 catcaaagaa gagcccaaaa ttgacctcaa ggtctacagt ctgctaaacc cagactctaa 4561 aatgggagaa acagttcccg agagcatagt caagagagag aaacttgatg acaaggaagg 4621 gctgaaagat aaaatctgga ctgagagttc aactgatgac cttcgtggtg tggcttggag 4681 aggggcagac atcctacggg gtagtccatc ctacactcaa gcaagcttag gggtcttgac 4741 gcctgtgtcc agctcccaag agcagacaaa gactctgagg gacagcccca atgttgaagc 4801 tgcccacctg gcacgaccta tgtatggctt ggcagtagat accaagggct tcctgcaagg 4861 ggctcctgct ggcagcgaga agtctgcatc tcttacccag cagtatcctg cctccggaga 4921 gagcaagacc aaggatgaat cccagtcgct gttacggagg cgaagaggat ctggtgtttt 4981 ttgtgccaat tgcctgacca caaagacctc tctctggcga aagaatgcaa atggcggata 5041 tgtatgcaat gcatgtggtc tctaccagaa acttcactcg actcccaggc ctttaaacat 5101 cattaaacaa aacaacggcg agcagattat tagaagacgg acaagaaagc gccttaaccc 5161 agaggcactt caggctgagc agctcaacaa acagcagagg ggaagtgggg aggagcaggt 5221 caatggaagc cccttagaga ggaggtcaga agatcactta agtgaaagtc atccgagaga 5281 aattccactt ccgagcctga gtaaatacga agcccagggt tcattgacta aaagccattc 5341 tgctcagcag ccagtcctgg tcagccaagc tctggatatc cacaaaagga tgcagccttt 5401 gcacattcag ataaaaagtc ctcaggaaag tactggagat ccaggaaata gttcatccgt 5461 atctgatggg aaaggaagtt ctgaaagagg cagccccatc gaaaagtaca tgagacctgc 5521 aaaacacccc aactattctc caccaggcag ccccatcgaa aagtaccaat acccgctttt 5581 tggagttcct tttgtacata atgacttcca gagtgaagct gattggctac ggttctggag 5641 taaatataag ctctccgttc ctgggaatcc gcactacttg agtcatgtgc ctggcttacc 5701 aaatccttgc caaaactatg tgccttatcc caccttcaat ctgcctcctc atttctcagc 5761 tgttggatca gacaatgaca ttcctctaga tttggcgatc aagcattcca gacctgggcc 5821 aactgcaaat ggtgcctcca aggagaaaac caaggcacca ccaactgtaa aaaatgaagg 5881 tcccttgaat gtagttaaga cggagaaagt tgacagaagc actcaagatg aactttcaac 5941 gaaatgtgtg cactgtggca ttgtttttct ggatgaagtg atgtatgctt tgcatatgag 6001 ttgccatggt gacagtggac ctttccagtg cagcatatgc cagcatcttt gcacggacaa 6061 atatgacttc acaacacata tccagagggg cctgcatagg aacaatgcac aagcagaaaa 6121 aaatggaaaa cctaaagagg gatccGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA 6181 TGACATCGAC TACAAGGATG ACGATGACAA GTGAgcggcc gcGaaggatc tgcgatcgct 6241 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag 6301 gggtcggcaa ttgaacgggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg 6361 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag 6421 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacagctg aagcttcgag 6481 gggctcgcat ctctccttca cgcgcccgcc gccctacctg aggccgccat ccacgccggt 6541 tgagtcgcgt tctgccgcct cccgcctgtg gtgcctcctg aactgcgtcc gccgtctagg 6601 taagtttaaa gctcaggtcg agaccgggcc tttgtccggc gctcccttgg agcctaccta 6661 gactcagccg gctctccacg ctttgcctga ccctgcttgc tcaactctac gtctttgttt 6721 cgttttctgt tctgcgccgt tacagatcca agctgtgacc ggcgcctacg ctagacgcca 6781 ccatggagag cgacgagagc ggcctgcccg ccatggagat cgagtgccgc atcaccggca 6841 ccctgaacgg cgtggagttc gagctggtgg gcggcggaga gggcaccccc aagcagggcc 6901 gcatgaccaa caagatgaag agcaccaaag gcgccctgac cttcagcccc tacctgctga 6961 gccacgtgat gggctacggc ttctaccact tcggcaccta ccccagcggc tacgagaacc 7021 ccttcctgca cgccatcaac aacggcggct acaccaacac ccgcatcgag aagtacgagg 7081 acggcggcgt gctgcacgtg agcttcagct accgctacga ggccggccgc gtgatcggcg 7141 acttcaaggt ggtgggcacc ggcttccccg aggacagcgt gatcttcacc gacaagatca 7201 tccgcagcaa cgccaccgtg gagcacctgc accccatggg cgataacgtg ctggtgggca 7261 gcttcgcccg caccttcagc ctgcgcgacg gcggctacta cagcttcgtg gtggacagcc 7321 acatgcactt caagagcgcc atccacccca gcatcctgca gaacgggggc cccatgttcg 7381 ccttccgccg cgtggaggag ctgcacagca acaccgagct gggcatcgtg gagtaccagc 7441 acgccttcaa gacccccatc gccttcgcca gatcccgcgc tcagtcgtcc aattctgccg 7501 tggacggcac cgccggaccc ggctccaccg gatctcgcga gggcagagga agtcttctaa 7561 catgcggtga cgtggaggag aatcccggcc ctatgaccga gtacaagccc acggtgcgcc 7621 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact 7681 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc 7741 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg 7801 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg 7861 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg 7921 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg 7981 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg 8041 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct 8101 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca 8161 cctggtgcat gacccgcaag cccggtgcct gaaatcaacc tctggattac aaaatttgtg 8221 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt 8281 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata 8341 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg 8401 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc 8461 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct 8521 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt 8581 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg 8641 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc 8701 tgctgccggc tctgcggcct cttccgcgtc tccgccttcg ccctcagacg agtcggatct 8761 ccctttggcc gcctccccgc ctggtacctt taagaccaat gacttacaag gcagctgtag 8821 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa 8881 aataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg 8941 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc 9001 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct 9061 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt 9121 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata 9181 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 9241 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat 9301 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt 9361 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg 9421 cttttttgga ggcctagact tttgcagaga cggcccaaat tcgtaatcat ggtcatagct 9481 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 9541 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 9601 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 9661 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 9721 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 9781 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 9841 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 9901 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 9961 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 10021 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 10081 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 10141 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 10201 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 10261 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 10321 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 10381 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 10441 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 10501 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 10561 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 10621 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 10681 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 10741 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 10801 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 10861 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 10921 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 10981 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 11041 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 11101 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 11161 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 11221 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 11281 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 11341 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 11401 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 11461 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 11521 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 11581 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 11641 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg 11701 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 11761 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 11821 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 11881 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc 11941 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 12001 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca 12061 gtcacgacgt tgtaaaacga cggccagtgc caagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.