pCMV-MET-ECFP-Neo plasmid

pCMV-MET-ECFP-Neo plasmid

PVT94574

pCMV-MET-ECFP-Neo plasmid Information

Plasmid name: pCMV-MET(human)-ECFP-Neo
Alias: NM_000245.4;AUTS9; c-Met; DA11; DFNB97; HGFR; RCCP2
Gene lenght: 4170bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-MET-ECFP-Neo plasmid Description

pCMV-MET-ECFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-MET-ECFP-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggactcag atgaattcgc caccatgaag gcccccgctg tgcttgcacc tggcatcctc 661 gtgctcctgt ttaccttggt gcagaggagc aatggggagt gtaaagaggc actagcaaag 721 tccgagatga atgtgaatat gaagtatcag cttcccaact tcaccgcgga aacacccatc 781 cagaatgtca ttctacatga gcatcacatt ttccttggtg ccactaacta catttatgtt 841 ttaaatgagg aagaccttca gaaggttgct gagtacaaga ctgggcctgt gctggaacac 901 ccagattgtt tcccatgtca ggactgcagc agcaaagcca atttatcagg aggtgtttgg 961 aaagataaca tcaacatggc tctagttgtc gacacctact atgatgatca actcattagc 1021 tgtggcagcg tcaacagagg gacctgccag cgacatgtct ttccccacaa tcatactgct 1081 gacatacagt cggaggttca ctgcatattc tccccacaga tagaagagcc cagccagtgt 1141 cctgactgtg tggtgagcgc cctgggagcc aaagtccttt catctgtaaa ggaccggttc 1201 atcaacttct ttgtaggcaa taccataaat tcttcttatt tcccagatca tccattgcat 1261 tcgatatcag tgagaaggct aaaggaaacg aaagatggtt ttatgttttt gacggaccag 1321 tcctacattg atgttttacc tgagttcaga gattcttacc ccattaagta tgtccatgcc 1381 tttgaaagca acaattttat ttacttcttg acggtccaaa gggaaactct agatgctcag 1441 acttttcaca caagaataat caggttctgt tccataaact ctggattgca ttcctacatg 1501 gaaatgcctc tggagtgtat tctcacagaa aagagaaaaa agagatccac aaagaaggaa 1561 gtgtttaata tacttcaggc tgcgtatgtc agcaagcctg gggcccagct tgctagacaa 1621 ataggagcca gcctgaatga tgacattctt ttcggggtgt tcgcacaaag caagccagat 1681 tctgccgaac caatggatcg atctgccatg tgtgcattcc ctatcaaata tgtcaacgac 1741 ttcttcaaca agatcgtcaa caaaaacaat gtgagatgtc tccagcattt ttacggaccc 1801 aatcatgagc actgctttaa taggacactt ctgagaaatt catcaggctg tgaagcgcgc 1861 cgtgatgaat atcgaacaga gtttaccaca gctttgcagc gcgttgactt attcatgggt 1921 caattcagcg aagtcctctt aacatctata tccaccttca ttaaaggaga cctcaccata 1981 gctaatcttg ggacatcaga gggtcgcttc atgcaggttg tggtttctcg atcaggacca 2041 tcaacccctc atgtgaattt tctcctggac tcccatccag tgtctccaga agtgattgtg 2101 gagcatacat taaaccaaaa tggctacaca ctggttatca ctgggaagaa gatcacgaag 2161 atcccattga atggcttggg ctgcagacat ttccagtcct gcagtcaatg cctctctgcc 2221 ccaccctttg ttcagtgtgg ctggtgccac gacaaatgtg tgcgatcgga ggaatgcctg 2281 agcgggacat ggactcaaca gatctgtctg cctgcaatct acaaggtttt cccaaatagt 2341 gcaccccttg aaggagggac aaggctgacc atatgtggct gggactttgg atttcggagg 2401 aataataaat ttgatttaaa gaaaactaga gttctccttg gaaatgagag ctgcaccttg 2461 actttaagtg agagcacgat gaatacattg aaatgcacag ttggtcctgc catgaataag 2521 catttcaata tgtccataat tatttcaaat ggccacggga caacacaata cagtacattc 2581 tcctatgtgg atcctgtaat aacaagtatt tcgccgaaat acggtcctat ggctggtggc 2641 actttactta ctttaactgg aaattaccta aacagtggga attctagaca catttcaatt 2701 ggtggaaaaa catgtacttt aaaaagtgtg tcaaacagta ttcttgaatg ttatacccca 2761 gcccaaacca tttcaactga gtttgctgtt aaattgaaaa ttgacttagc caaccgagag 2821 acaagcatct tcagttaccg tgaagatccc attgtctatg aaattcatcc aaccaaatct 2881 tttattagtg gtgggagcac aataacaggt gttgggaaaa acctgaattc agttagtgtc 2941 ccgagaatgg tcataaatgt gcatgaagca ggaaggaact ttacagtggc atgtcaacat 3001 cgctctaatt cagagataat ctgttgtacc actccttccc tgcaacagct gaatctgcaa 3061 ctccccctga aaaccaaagc ctttttcatg ttagatggga tcctttccaa atactttgat 3121 ctcatttatg tacataatcc tgtgtttaag ccttttgaaa agccagtgat gatctcaatg 3181 ggcaatgaaa atgtactgga aattaaggga aatgatattg accctgaagc agttaaaggt 3241 gaagtgttaa aagttggaaa taagagctgt gagaatatac acttacattc tgaagccgtt 3301 ttatgcacgg tccccaatga cctgctgaaa ttgaacagcg agctaaatat agagtggaag 3361 caagcaattt cttcaaccgt ccttggaaaa gtaatagttc aaccagatca gaatttcaca 3421 ggattgattg ctggtgttgt ctcaatatca acagcactgt tattactact tgggtttttc 3481 ctgtggctga aaaagagaaa gcaaattaaa gatctgggca gtgaattagt tcgctacgat 3541 gcaagagtac acactcctca tttggatagg cttgtaagtg cccgaagtgt aagcccaact 3601 acagaaatgg tttcaaatga atctgtagac taccgagcta cttttccaga agatcagttt 3661 cctaattcat ctcagaacgg ttcatgccga caagtgcagt atcctctgac agacatgtcc 3721 cccatcctaa ctagtgggga ctctgatata tccagtccat tactgcaaaa tactgtccac 3781 attgacctca gtgctctaaa tccagagctg gtccaggcag tgcagcatgt agtgattggg 3841 cccagtagcc tgattgtgca tttcaatgaa gtcataggaa gagggcattt tggttgtgta 3901 tatcatggga ctttgttgga caatgatggc aagaaaattc actgtgctgt gaaatccttg 3961 aacagaatca ctgacatagg agaagtttcc caatttctga ccgagggaat catcatgaaa 4021 gattttagtc atcccaatgt cctctcgctc ctgggaatct gcctgcgaag tgaagggtct 4081 ccgctggtgg tcctaccata catgaaacat ggagatcttc gaaatttcat tcgaaatgag 4141 actcataatc caactgtaaa agatcttatt ggctttggtc ttcaagtagc caaaggcatg 4201 aaatatcttg caagcaaaaa gtttgtccac agagacttgg ctgcaagaaa ctgtatgctg 4261 gatgaaaaat tcacagtcaa ggttgctgat tttggtcttg ccagagacat gtatgataaa 4321 gaatactata gtgtacacaa caaaacaggt gcaaagctgc cagtgaagtg gatggctttg 4381 gaaagtctgc aaactcaaaa gtttaccacc aagtcagatg tgtggtcctt tggcgtgctc 4441 ctctgggagc tgatgacaag aggagcccca ccttatcctg acgtaaacac ctttgatata 4501 actgtttact tgttgcaagg gagaagactc ctacaacccg aatactgccc agacccctta 4561 tatgaagtaa tgctaaaatg ctggcaccct aaagccgaaa tgcgcccatc cttttctgaa 4621 ctggtgtccc ggatatcagc gatcttctct actttcattg gggagcacta tgtccatgtg 4681 aacgctactt atgtgaacgt aaaatgtgtc gctccgtatc cttctctgtt gtcatcagaa 4741 gataacgctg atgatgaggt ggacacacga ccagcctcct tctgggagac atcactcgag 4801 ggtggcggag gttctggcgg tggaggttca ctggatccac cggtcgccac catggtgagc 4861 aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta 4921 aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg 4981 accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc 5041 accctgacct ggggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac 5101 ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac 5161 gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc 5221 atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag 5281 tacaactaca tcagccacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag 5341 gccaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac 5401 cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc 5461 acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag 5521 ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta gcggccgcga 5581 ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc 5641 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt 5701 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 5761 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta 5821 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 5881 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 5941 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 6001 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 6061 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 6121 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 6181 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 6241 acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt 6301 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 6361 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag 6421 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 6481 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 6541 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 6601 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 6661 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 6721 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 6781 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 6841 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 6901 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 6961 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 7021 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 7081 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 7141 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 7201 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 7261 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 7321 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 7381 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 7441 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 7501 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 7561 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 7621 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 7681 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 7741 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 7801 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 7861 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 7921 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 7981 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 8041 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 8101 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 8161 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 8221 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 8281 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 8341 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 8401 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 8461 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 8521 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 8581 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 8641 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 8701 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 8761 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 8821 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 8881 gataaccgta ttaccgccat gcat

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.