pCMV-EGFP-PXN-2-Neo plasmid

pCMV-EGFP-PXN-2-Neo plasmid

PVT94548

pCMV-EGFP-PXN-2-Neo plasmid Information

Plasmid name: pCMV-EGFP-PXN(human)-2-Neo
Alias: NM_002859.4
Gene lenght: 1674bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pEGFP-C-5'
Reverse primer:
Copy number: High
Induction:

pCMV-EGFP-PXN-2-Neo plasmid Description

pCMV-EGFP-PXN-2-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-EGFP-PXN-2-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 601 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 661 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 721 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 781 ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc 841 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 901 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 961 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1021 atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat catggccgac 1081 aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc 1141 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1201 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1261 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1321 ctgtacaagt ccggactcag atctcgagGC GGCGGAGGGA GTGGAGGAGG GGGAAGCaag 1381 cttatggacg acctcgacgc cctgctggcg gacttggagt ctaccacctc ccacatctcc 1441 aaacggcctg tgttcttgtc ggaggagacc ccctactcat acccaactgg aaaccacaca 1501 taccaggaga ttgccgtgcc accccccgtc cccccacccc cgtccagcga ggccctcaat 1561 ggcacaatcc ttgacccctt agaccagtgg cagcccagca gctcccgatt catccaccag 1621 cagcctcagt cctcatcacc tgtgtacggc tccagtgcca aaacttccag tgtctccaac 1681 cctcaggaca gtgttggctc tccgtgctcc cgagtgggtg aggaggagca cgtctacagc 1741 ttccccaaca agcagaaatc agctgagcct tcacccaccg taatgagcac gtccctgggc 1801 agcaaccttt ctgaactcga ccgcctgctg ctggaactga acgctgtaca gcataacccg 1861 ccaggcttcc ctgcagatga ggccaactca agccccccgc ttcctggggc cctgagcccc 1921 ctctatggtg tcccagagac taacagcccc ttgggaggca aagctgggcc cctgacgaaa 1981 gagaagccta agcggaatgg gggccggggc ctggaggacg tgcggcccag tgtggagagt 2041 ctcttggatg aactggagag ctccgtgccc agccccgtcc ctgccatcac tgtgaaccag 2101 ggcgagatga gcagcccgca gcgcgtcacc tccacccaac agcagacacg catctcggcc 2161 tcctctgcca ccagggagct ggacgagctg atggcttcgc tgtcggattt caagttcatg 2221 gcccagggga agacagggag cagctcaccc cctggggggc ccccgaagcc cgggagccag 2281 ctggacagca tgctggggag cctgcagtct gacctgaaca agctgggggt cgccacagtc 2341 gccaaaggag tctgcggggc ctgcaagaag cccatcgccg ggcaggttgt gaccgccatg 2401 gggaagacgt ggcaccccga gcacttcgtc tgcacccact gccaggagga gatcggatcc 2461 cggaacttct tcgagcggga tggacagccc tactgtgaaa aggactacca caacctcttc 2521 tccccgcgct gctactactg caacggcccc atcctggata aagtggtgac agcccttgac 2581 cggacgtggc accctgaaca cttcttctgt gcacagtgtg gagccttctt tggtcccgaa 2641 gggttccacg agaaggacgg caaggcctac tgtcgcaagg actacttcga catgttcgca 2701 cccaagtgtg gcggctgcgc ccgggccatc ctggagaact atatctcagc cctcaacacg 2761 ctgtggcatc ctgagtgctt tgtgtgccgg gaatgcttca cgccattcgt gaacggcagc 2821 ttcttcgagc acgacgggca gccctactgt gaggtgcact accacgagcg gcgcggctcg 2881 ctgtgttctg gctgccagaa gcccatcacc ggccgctgca tcaccgccat ggccaagaag 2941 ttccaccccg agcacttcgt ctgtgccttc tgcctcaagc agctcaacaa gggcaccttc 3001 aaggagcaga acgacaagcc ttactgtcag aactgcttcc tcaagctctt ctgctaggga 3061 tccaccggat ctagataact gatcataatc agccatacca catttgtaga ggttttactt 3121 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt 3181 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat 3241 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat 3301 gtatcttaac gcgtaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg 3361 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa 3421 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa 3481 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg 3541 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa 3601 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa 3661 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct 3721 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt caggtggcac 3781 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat 3841 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag 3901 tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca 3961 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt 4021 ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca 4081 gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc 4141 cattctccgc cccatggctg actaattttt tttatttatg cagaggccga ggccgcctcg 4201 gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa 4261 gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc 4321 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat 4381 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt 4441 caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc ggctatcgtg 4501 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag 4561 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc 4621 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc 4681 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga 4741 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga 4801 actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg 4861 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg 4921 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc 4981 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc 5041 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg 5101 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc 5161 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc 5221 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag gctaactgaa 5281 acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa aagacagaat 5341 aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca gggctggcac 5401 tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc ttccttttcc 5461 ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt cggggcggca 5521 ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa acttcatttt 5581 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa 5641 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga 5701 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg 5761 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc 5821 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag 5881 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc 5941 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg 6001 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac 6061 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga 6121 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt 6181 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag 6241 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg 6301 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta 6361 tcccctgatt ctgtggataa ccgtattacc gccatgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.