pTac-ACSL3-GST plasmid

pTac-ACSL3-GST plasmid

PVT94458

pTac-ACSL3-GST plasmid Information

Plasmid name: pTac-ACSL3(human)-GST
Alias: NM_004457.5;ACS3; FACL3; LACS 3; LACS3; PRO2194
Gene lenght: 2160bp
Host: E.coli
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling:
Promoter: Tac
Replicon: pBR322
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: Tac-P
Reverse primer:
Copy number: Low
Induction: IPTG Induction

pTac-ACSL3-GST plasmid Description

pTac-ACSL3-GST is a plasmid suitable for protein expression in Escherichia coli.

pTac-ACSL3-GST plasmid Sequence

1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg 61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt 121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc 181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca 241 cacaggaaac agtattcgga tatgaataac cacgtgtctt caaaaccatc taccatgaag 301 ctaaaacata ccatcaaccc tattctttta tattttatac attttctaat atcactttat 361 actattttaa catacattcc gttttatttt ttctccgagt caagacaaga aaaatcaaac 421 cgaattaaag caaagcctgt aaattcaaaa cctgattctg catacagatc tgttaatagt 481 ttggatggtt tggcttcagt attataccct ggatgtgata ctttagataa agtttttaca 541 tatgcaaaaa acaaatttaa gaacaaaaga ctcttgggaa cacgtgaagt tttaaatgag 601 gaagatgaag tacaaccaaa tggaaaaatt tttaaaaagg ttattcttgg acagtataat 661 tggctttcct atgaagatgt ctttgttcga gcctttaatt ttggaaatgg attacagatg 721 ttgggtcaga aaccaaagac caacatcgcc atcttctgtg agaccagggc cgagtggatg 781 atagctgcac aggcgtgttt tatgtataat tttcagcttg ttacattata tgccactcta 841 ggaggtccag ccattgttca tgcattaaat gaaacagagg tgaccaacat cattactagt 901 aaagaactct tacaaacaaa gttgaaggat atagtttctt tggtcccacg cctgcggcac 961 atcatcactg ttgatggaaa gccaccgacc tggtccgagt tccccaaggg catcattgtg 1021 cataccatgg ctgcagtgga ggccctggga gccaaggcca gcatggaaaa ccaacctcat 1081 agcaaaccat tgccctcaga tattgcagta atcatgtaca caagtggatc cacaggactt 1141 ccaaagggag tcatgatctc acatagtaac attattgctg gtataactgg gatggcagaa 1201 aggattccag aactaggaga ggaagatgtc tacattggat atttgcctct ggcccatgtt 1261 ctagaattaa gtgctgagct tgtctgtctt tctcacggat gccgcattgg ttactcttca 1321 ccacagactt tagcagatca gtcttcaaaa attaaaaaag gaagcaaagg ggatacatcc 1381 atgttgaaac caacactgat ggcagcagtt ccggaaatca tggatcggat ctacaaaaat 1441 gtcatgaata aagtcagtga aatgagtagt tttcaacgta atctgtttat tctggcctat 1501 aattacaaaa tggaacagat ttcaaaagga cgtaatactc cactgtgcga cagctttgtt 1561 ttccggaaag ttcgaagctt gctaggggga aatattcgtc tcctgttgtg tggtggcgct 1621 ccactttctg caaccacgca gcgattcatg aacatctgtt tctgctgtcc tgttggtcag 1681 ggatacgggc tcactgaatc tgctggggct ggaacaattt ccgaagtgtg ggactacaat 1741 actggcagag tgggagcacc attagtttgc tgtgaaatca aattaaaaaa ctgggaggaa 1801 ggtggatact ttaatactga taagccacac cccaggggtg aaattcttat tgggggccaa 1861 agtgtgacaa tggggtacta caaaaatgaa gcaaaaacaa aagctgattt ctttgaagat 1921 gaaaatggac aaaggtggct ctgtactggg gatattggag agtttgaacc cgatggatgc 1981 ttaaagatta ttgatcgtaa aaaggacctt gtaaaactac aggcagggga atatgtttct 2041 cttgggaaag tagaggcagc tttgaagaat cttccactag tagataacat ttgtgcatat 2101 gcaaacagtt atcattctta tgtcattgga tttgttgtgc caaatcaaaa ggaactaact 2161 gaactagctc gaaagaaagg acttaaaggg acttgggagg agctgtgtaa cagttgtgaa 2221 atggaaaatg aggtacttaa agtgctttcc gaagctgcta tttcagcaag tctggaaaag 2281 tttgaaattc cagtaaaaat tcgtttgagt cctgaaccgt ggacccctga aactggtctg 2341 gtgacagatg ccttcaagct gaaacgcaaa gagcttaaaa cacattacca ggcggacatt 2401 gagcgaatgt atggaagaaa aCTCGAGCTG GTGCCGCGCG GCAGCtcaGG AGGAAGCgga 2461 gggagtatgt cccctatact aggttattgg aaaattaagg gccttgtgca acccactcga 2521 cttcttttgg aatatcttga agaaaaatat gaagagcatt tgtatgagcg cgatgaaggt 2581 gataaatggc gaaacaaaaa gtttgaattg ggtttggagt ttcccaatct tccttattat 2641 attgatggtg atgttaaatt aacacagtct atggccatca tacgttatat agctgacaag 2701 cacaacatgt tgggtggttg tccaaaagag cgtgcagaga tttcaatgct tgaaggagcg 2761 gttttggata ttagatacgg tgtttcgaga attgcatata gtaaagactt tgaaactctc 2821 aaagttgatt ttcttagcaa gctacctgaa atgctgaaaa tgttcgaaga tcgtttatgt 2881 cataaaacat atttaaatgg tgatcatgta acccatcctg acttcatgtt gtatgacgct 2941 cttgatgttg ttttatacat ggacccaatg tgcctggatg cgttcccaaa attagtttgt 3001 tttaaaaaac gtattgaagc tatcccacaa attgataagt acttgaaatc cagcaagtat 3061 atagcatggc ctttgcaggg ctggcaagcc acgtttggtg gtggcgacca tcctccaaaa 3121 tagcggccgc atcgtgactg actgacgatc tgcctcgcgc gtttcggtga tgacggtgaa 3181 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 3241 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg 3301 acccagtcac gtagcgatag cggagtgtat aattcttgaa gacgaaaggg cctcgtgata 3361 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact 3421 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg 3481 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt 3541 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct 3601 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca 3661 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc 3721 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc 3781 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg 3841 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta 3901 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc 3961 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt 4021 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg 4081 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct 4141 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc 4201 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct 4261 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac 4321 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc 4381 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat 4441 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg 4501 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc 4561 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa 4621 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag 4681 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta 4741 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta 4801 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag 4861 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg 4921 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg 4981 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag 5041 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc 5101 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa 5161 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg 5221 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct 5281 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa 5341 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataaat 5401 tccgacacca tcgaatggtg caaaaccttt cgcggtatgg catgatagcg cccggaagag 5461 agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc 5521 ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa 5581 acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca 5641 caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg 5701 cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc 5761 gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat 5821 cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc 5881 attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag 5941 acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat 6001 ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg 6061 gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata 6121 gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg 6181 aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca 6241 atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac 6301 gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt 6361 cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg 6421 aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat 6481 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 6541 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 6601 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 6661 ataacaattt cacacaggaa acagctatga ccatgattac ggattcactg gccgtcgttt 6721 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc 6781 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt 6841 tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg 6901 aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc 6961 agatgcacgg ttacgatgcg cccatctaca ccaacgtaac ctatcccatt acggtcaatc 7021 cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg 7081 aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttgga att

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.