pLV3-CMV-GLS-2-3×FLAG-CopGFP-P

pLV3-CMV-GLS-2-3×FLAG-CopGFP-Puro plasmid

PVT94452

pLV3-CMV-GLS-2-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-GLS(human)-2-3×FLAG-CopGFP-Puro
Alias: BC038507.1;GLS1;KIAA0838
Gene lenght: 2007bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pLV3-CMV-GLS-2-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-GLS-2-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-GLS-2-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc gccaccatga tgcggctgcg aggctcgggg 2341 atgctgcggg acctgctcct gcggtcgccc gccggcgtga gcgcgactct gcggcgggca 2401 cagcccttgg tcaccctgtg ccggcgtccc cgaggcgggg gacggccggc cgcgggcccg 2461 gctgccgccg cgcgactcca cccgtggtgg ggcgggggcg gctggccggc ggagcccctc 2521 gcgcggggcc tgtccagctc tccttcggag atcttgcagg agctgggcaa ggggagcacg 2581 catccgcagc ccggggtgtc gccacccgct gccccggcgg cgcccggccc caaggacggc 2641 cccggggaga cggacgcgtt tggcaacagc gagggcaaag agctggtggc ctcaggtgaa 2701 aataaaataa aacagggtct gttacctagc ttggaagatt tgctgttcta tacaattgct 2761 gaaggacaag agaaaatacc tgttcataaa tttattacag cactcaaatc tacaggattg 2821 cgaacgtctg atcccaggtt gaaagagtgt atggatatgt taagattaac tcttcaaaca 2881 acatcagatg gtgtcatgct agacaaagat ctttttaaaa aatgtgttca gagcaacatt 2941 gttttgttga cacaagcatt tagaagaaag tttgtgattc ctgactttat gtcttttacc 3001 tcacacattg atgagttata tgaaagtgct aaaaagcagt ctggaggaaa ggttgcagat 3061 tatattcctc aactggccaa attcagtccc gatttgtggg gtgtgtctgt ttgtacagta 3121 gatggacaga ggcattctac tggagatacc aaagttccct tctgtcttca gtcctgtgta 3181 aaacctttga aatatgccat tgctgttaat gatcttggaa ctgaatatgt gcatcgatat 3241 gttggaaaag agccgagtgg actaagattc aacaaactat ttttgaatga agatgataaa 3301 ccacataatc ctatggtaaa tgctggagca attgttgtga cttcactaat aaagcaagga 3361 gtaaataatg ctgaaaaatt tgactatgtc atgcagtttt tgaataagat ggctggtaat 3421 gaatatgttg gattcagtaa tgcaacgttt cagtctgaaa gagaaagtgg agatcgaaat 3481 tttgcaatag gatattactt aaaagaaaag aagtgttttc cagaaggcac agacatggtt 3541 ggtatattag acttctactt ccagctgtgc tccattgaag tgacttgtga atcagccagt 3601 gtgatggctg cgacactggc taatggtggt ttctgcccaa ttactggtga aagagtactg 3661 agccctgaag cagttcgaaa tacattgagt ttgatgcatt cctgtggcat gtatgacttc 3721 tcagggcagt ttgctttcca tgttggtctt cctgcaaaat ctggagttgc tgggggcatt 3781 cttttagttg tccccaatgt tatgggtatg atgtgctggt ctcctcctct ggataagatg 3841 ggcaacagtg ttaagggaat tcacttttgt cacgatcttg tttctctgtg taatttccat 3901 aactatgata atttgagaca ctttgcaaaa aaacttgatc ctcgaagaga aggtggtgat 3961 caaagggtaa agtcagtgat aaatcttttg tttgctgcat atactggaga tgtgtctgca 4021 cttcgaagat ttgctttgtc agctatggac atggaacagc gggactatga ttctagaaca 4081 gcactccatg tagctgctgc agagggtcat gttgaagttg ttaaattttt gctggaagcc 4141 tgcaaagtaa accctttccc caaggacagg tggaataaca ctcccatgga tgaagcactg 4201 cactttggac accatgatgt atttaaaatt ctccaagaat accaagtcca gtacacacct 4261 caaggagatt ctgacaacgg gaaggaaaat caaacAgtcc ataagaatct tgatggattg 4321 ttgggatccG ACTACAAAGA CCATGACGGT GATTATAAAG ATCATGACAT CGACTACAAG 4381 GATGACGATG ACAAGtagcg gccgcGaagg atctgcgatc gctccggtgc ccgtcagtgg 4441 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacg 4501 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc 4561 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt 4621 tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc gaggggctcg catctctcct 4681 tcacgcgccc gccgccctac ctgaggccgc catccacgcc ggttgagtcg cgttctgccg 4741 cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct aggtaagttt aaagctcagg 4801 tcgagaccgg gcctttgtcc ggcgctccct tggagcctac ctagactcag ccggctctcc 4861 acgctttgcc tgaccctgct tgctcaactc tacgtctttg tttcgttttc tgttctgcgc 4921 cgttacagat ccaagctgtg accggcgcct acgctagacg ccaccatgga gagcgacgag 4981 agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag 5041 ttcgagctgg tgggcggcgg agagggcacc cccaagcagg gccgcatgac caacaagatg 5101 aagagcacca aaggcgccct gaccttcagc ccctacctgc tgagccacgt gatgggctac 5161 ggcttctacc acttcggcac ctaccccagc ggctacgaga accccttcct gcacgccatc 5221 aacaacggcg gctacaccaa cacccgcatc gagaagtacg aggacggcgg cgtgctgcac 5281 gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc 5341 accggcttcc ccgaggacag cgtgatcttc accgacaaga tcatccgcag caacgccacc 5401 gtggagcacc tgcaccccat gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc 5461 agcctgcgcg acggcggcta ctacagcttc gtggtggaca gccacatgca cttcaagagc 5521 gccatccacc ccagcatcct gcagaacggg ggccccatgt tcgccttccg ccgcgtggag 5581 gagctgcaca gcaacaccga gctgggcatc gtggagtacc agcacgcctt caagaccccc 5641 atcgccttcg ccagatcccg cgctcagtcg tccaattctg ccgtggacgg caccgccgga 5701 cccggctcca ccggatctcg cgagggcaga ggaagtcttc taacatgcgg tgacgtggag 5761 gagaatcccg gccctatgac cgagtacaag cccacggtgc gcctcgccac ccgcgacgac 5821 gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc cacgcgccac 5881 accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact cttcctcacg 5941 cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc 6001 tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg 6061 gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct cctggcgccg 6121 caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag 6181 ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg 6241 gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga gcggctcggc 6301 ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg catgacccgc 6361 aagcccggtg cctgaaatca acctctggat tacaaaattt gtgaaagatt gactggtatt 6421 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat 6481 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct 6541 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct 6601 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc 6661 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg 6721 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa ATCATCGTCC 6781 TTTCCTtggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac 6841 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg 6901 cctcttccgc gtctTcgcct tcgccctcag acgagtcgga tctccctttg gGccgcctcc 6961 ccgcctggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt 7021 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaaGataag atctgctttt 7081 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact 7141 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc 7201 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa 7261 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa 7321 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag 7381 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 7441 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctaTCCCGCC CCTAACTCCG 7501 CCCAtcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt 7561 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga 7621 ggaggctttt ttggaggcct agacttttgc agagaccaaa ttcgtaatca tgtcatagct 7681 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat 7741 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 7801 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 7861 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 7921 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 7981 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 8041 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 8101 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 8161 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 8221 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg 8281 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 8341 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 8401 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 8461 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 8521 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 8581 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 8641 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 8701 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 8761 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 8821 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 8881 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 8941 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 9001 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 9061 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 9121 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 9181 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 9241 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 9301 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 9361 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 9421 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 9481 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 9541 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 9601 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 9661 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 9721 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 9781 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 9841 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg 9901 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg 9961 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg 10021 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat 10081 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc 10141 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca 10201 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca 10261 gtcacgacgt tgtaaaacga cggccagtgc caagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.