pLV3-CMV-ATRX-3×FLAG-Puro plas

pLV3-CMV-ATRX-3×FLAG-Puro plasmid

PVT94451

pLV3-CMV-ATRX-3×FLAG-Puro plasmid Information

Plasmid name: pLV3-CMV-ATRX(human)-3×FLAG-Puro
Alias: NM_000489.6;JMS; MRX52; RAD54; RAD54L; XH2; XNP;ZNF-HX;
Gene lenght: 7476bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): BL21(DE3)
Growth Temperature: 30°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: Low
Induction:

pLV3-CMV-ATRX-3×FLAG-Puro plasmid Description

pLV3-CMV-ATRX-3×FLAG-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-ATRX-3×FLAG-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCgccac catgaccgct gagcccatga gtgaaagcaa gttgaataca 2341 ttggtgcaga agcttcatga cttccttgca cactcatcag aagaatctga agaaacaagt 2401 tctcctccac gacttgcaat gaatcaaaac acagataaaa tcagtggttc tggaagtaac 2461 tctgatatga tggaaaacag caaggaagag ggaactagct cttcagaaaa atccaagtct 2521 tcaggatcgt cacgatcaaa gaggaaacct tcaattgtaa caaagtatgt agaatcagat 2581 gatgaaaaac ctttggatga tgaaactgta aatgaagatg cgtctaatga aaattcagaa 2641 aatgatatta ctatgcagag cttgccaaaa ggtacagtga ttgtacagcc agagccagtg 2701 ctgaatgaag acaaagatga ttttaaaggg cctgaattta gaagcagaag taaaatgaaa 2761 actgaaaatc tcaaaaaacg cggagaagat gggcttcatg ggattgtgag ctgcactgct 2821 tgtggacaac aggtcaatca ttttcaaaaa gattccattt atagacaccc ttcattgcaa 2881 gttcttattt gtaagaattg ctttaagtat tacatgagtg atgatattag ccgtgactca 2941 gatggaatgg atgaacaatg taggtggtgt gcggaaggtg gaaacttgat ttgttgtgac 3001 ttttgccata atgctttctg caagaaatgc attctacgca accttggtcg aaaggagttg 3061 tccacaataa tggatgaaaa caaccaatgg tattgctaca tttgtcaccc agagcctttg 3121 ttggacttgg tcactgcatg taacagcgta tttgagaatt tagaacagtt gttgcagcaa 3181 aataagaaga agataaaagt tgacagtgaa aagagtaata aagtatatga acatacatcc 3241 agattttctc caaagaagac tagttcaaat tgtaatggag aagaaaagaa attagatgat 3301 tcctgttctg gctctgtaac ctactcttat tccgcactaa ttgtgcccaa agagatgatt 3361 aagaaggcaa aaaaactgat tgagaccaca gccaacatga actccagtta tgttaaattt 3421 ttaaagcagg caacagataa ttcagaaatc agttctgcta caaaattacg tcagcttaag 3481 gcttttaagt ctgtgttggc tgatattaag aaggctcatc ttgcattgga agaagactta 3541 aattccgagt ttcgagcgat ggatgctgta aacaaagaga aaaataccaa agagcataaa 3601 gtcatagatg ctaagtttga aacaaaagca cgaaaaggag aaaaaccttg tgctttggaa 3661 aagaaggata tttcaaagtc agaagctaaa ctttcaagaa aacaggtaga tagtgagcac 3721 atgcatcaga atgttccaac agaggaacaa agaacaaata aaagtaccgg tggtgaacat 3781 aagaaatctg atagaaaaga agaacctcaa tatgaacctg ccaacacttc tgaagattta 3841 gacatggata ttgtgtctgt tccttcctca gttccagaag acatttttga gaatcttgag 3901 actgctatgg aagttcagag ttcagttgat catcaagggg atggcagcag tggaactgaa 3961 caagaagtgg agagttcatc tgtaaaatta aatatttctt caaaagacaa cagaggaggt 4021 attaaatcaa aaactacagc taaagtaaca aaagaattat atgttaaact cactcctgtt 4081 tccctttcta attccccaat taaaggtgct gattgtcagg aagttccaca agataaagat 4141 ggctataaaa gttgtggtct gaaccccaag ttagagaaat gtggacttgg acaggaaaac 4201 agtgataatg agcatttggt tgaaaatgaa gtttcattac ttttagagga atctgatctt 4261 cgaagatccc cacgtgtaaa gactacaccc ttgaggcgac cgacagaaac taaccctgta 4321 acatctaatt cagatgaaga atgtaatgaa acagttaagg agaaacaaaa actatcagtt 4381 ccagtgagaa aaaaggataa gcgtaattct tctgacagtg ctatagataa tcctaagcct 4441 aataaattgc caaaatctaa gcaatcagag actgtggatc aaaattcaga ttctgatgaa 4501 atgctagcaa tcctcaaaga ggtgagcagg atgagtcaca gttcttcttc agatactgat 4561 attaatgaaa ttcatacaaa ccataagact ttgtatgatt taaagactca ggcggggaaa 4621 gatgataaag gaaaaaggaa acgaaaaagt tctacatctg gctcagattt tgatactaaa 4681 aagggcaaat cagctaagag ctctataatt tctaaaaaga aacgacaaac ccagtctgag 4741 tcttctaatt atgactcaga attagaaaaa gagataaaga gcatgagtaa aattggtgct 4801 gccagaacca ccaaaaaaag aattccaaat acaaaagatt ttgactcttc tgaagatgag 4861 aaacacagca aaaaaggaat ggataatcaa gggcacaaaa atttgaagac ctcacaagaa 4921 ggatcatctg atgatgctga aagaaaacaa gagagagaga ctttctcttc agcagaaggc 4981 acagttgata aagacacgac catcatggaa ttaagagatc gacttcctaa gaagcagcaa 5041 gcaagtgctt ccactgatgg tgtcgataag ctttctggga aagaggagag ttttacttct 5101 ttggaagtta gaaaagttgc tgaaactaaa gaaaagagca agcatctcaa aaccaaaaca 5161 tgtaaaaaag tacaggatgg cttatctgat attgcagaga aattcctaaa gaaagaccag 5221 agcgatgaaa cttctgaaga tgataaaaag cagagcaaaa agggaactga agaaaaaaag 5281 aaaccttcag actttaagaa aaaagtaatt aaaatggaac aacagtatga atcttcatct 5341 gatggcactg aaaagttacc tgagcgagaa gaaatttgtc attttcctaa gggcataaaa 5401 caaattaaga atggaacaac tgatggagaa aagaaaagta aaaaaataag agataaaact 5461 tctaaaaaga aggatgaatt atctgattat gctgagaagt caacagggaa aggagatagt 5521 tgtgactctt cagaggataa aaagagtaag aatggagcat atggtagaga gaagaaaagg 5581 tgcaagttgc ttggaaagag ttcaaggaag agacaagatt gttcatcatc tgatactgag 5641 aaatattcca tgaaagaaga tggttgtaac tcttctgata agagactgaa aagaatagaa 5701 ttgagggaaa gaagaaattt aagttcaaag agaaatacta aggaaataca aagtggctca 5761 tcatcatctg atgctgagga aagttctgaa gataataaaa agaagaagca aagaacttca 5821 tctaaaaaga aggcagtcat tgtcaaggag aaaaagagaa actccctaag aacaagcact 5881 aaaaggaagc aagctgacat tacatcctca tcttcttctg atatagaaga tgatgatcag 5941 aattctatag gtgagggaag cagcgatgaa cagaaaatta agcctgtgac tgaaaattta 6001 gtgctgtctt cacatactgg attttgccaa tcttcaggag atgaagcctt atctaaatca 6061 gtgcctgtca cagtggatga tgatgatgac gacaatgatc ctgagaatag aattgccaag 6121 aagatgcttt tagaagaaat taaagccaat ctttcctctg atgaggatgg atcttcagat 6181 gatgagccag aagaagggaa aaaaagaact ggaaaacaaa atgaagaaaa cccaggagat 6241 gaggaagcaa aaaatcaagt caattctgaa tcagattcag attctgaaga atctaagaag 6301 ccaagataca gacataggct tttgcggcac aaattgactg tgagtgacgg agaatctgga 6361 gaagaaaaaa agacaaagcc taaagagcat aaagaagtca aaggcagaaa cagaagaaag 6421 gtgagcagtg aagattcaga agattctgat tttcaggaat caggagttag tgaagaagtt 6481 agtgaatccg aagatgaaca gcggcccaga acaaggtctg caaagaaagc agagttggaa 6541 gaaaatcagc ggagctataa acagaaaaag aaaaggcgac gtattaaggt tcaagaagat 6601 tcatccagtg aaaacaagag taattctgag gaagaagagg aggaaaaaga agaggaggag 6661 gaagaggagg aggaggagga agaggaggag gaagatgaaa atgatgattc caagtctcct 6721 ggaaaaggca gaaagaaaat tcggaagatt cttaaagatg ataaactgag aacagaaaca 6781 caaaatgctc ttaaggaaga ggaagagaga cgaaaacgta ttgctgagag ggagcgtgag 6841 cgagaaaaat tgagagaggt gatagaaatt gaagatgctt cacccaccaa gtgtccaata 6901 acaaccaagt tggttttaga tgaagatgaa gaaaccaaag aacctttagt gcaggttcat 6961 agaaatatgg ttatcaaatt gaaaccccat caagtagatg gtgttcagtt tatgtgggat 7021 tgctgctgtg agtctgtgaa aaaaacaaag aaatctccag gttcaggatg cattcttgcc 7081 cactgtatgg gccttggtaa gactttacag gtggtaagtt ttcttcatac agttcttttg 7141 tgtgacaaac tggatttcag cacggcgtta gtggtttgtc ctcttaatac tgctttgaat 7201 tggatgaatg aatttgagaa gtggcaagag ggattaaaag atgatgagaa gcttgaggtt 7261 tctgaattag caactgtgaa acgtcctcag gagagaagct acatgctgca gaggtggcaa 7321 gaagatggtg gtgttatgat cataggctat gagatgtata gaaatcttgc tcaaggaagg 7381 aatgtgaaga gtcggaaact taaagaaata tttaacaaag ctttggttga tccaggccct 7441 gattttgttg tttgtgatga aggccatatt ctaaaaaatg aagcatctgc tgtttctaaa 7501 gctatgaatt ctatacgatc aaggaggagg attattttaa caggaacacc acttcaaaat 7561 aacctaattg agtatcattg tatggttaat tttatcaagg aaaatttact tggatccatt 7621 aaggagttca ggaatagatt tataaatcca attcaaaatg gtcagtgtgc agattctacc 7681 atggtagatg tcagagtgat gaaaaaacgt gctcacattc tctatgagat gttagctgga 7741 tgtgttcaga ggaaagatta tacagcatta acaaaattct tgcctccaaa acacgaatat 7801 gtgttagctg tgagaatgac ttctattcag tgcaagctct atcagtacta cttagatcac 7861 ttaacaggtg tgggcaataa tagtgaaggt ggaagaggaa aggcaggtgc aaagcttttc 7921 caagattttc agatgttaag tagaatatgg actcatcctt ggtgtttgca gctagactac 7981 attagcaaag aaaataaggg ttattttgat gaagacagta tggatgaatt tatagcctca 8041 gattctgatg aaacctccat gagtttaagc tccgatgatt atacaaaaaa gaagaaaaaa 8101 gggaaaaagg ggaaaaaaga tagtagctca agtggaagtg gcagtgacaa tgatgttgaa 8161 gtgattaagg tctggaattc aagatctcgg ggaggtggtg aaggaaatgt ggatgaaaca 8221 ggaaacaatc cttctgtttc tttaaaactg gaagaaagta aagctacttc ttcttctaat 8281 ccaagcagcc cagctccaga ctggtacaaa gattttgtta cagatgctga tgctgaggtt 8341 ttagagcatt ctgggaaaat ggtacttctc tttgaaattc ttcgaatggc agaggaaatt 8401 ggggataaag tccttgtttt cagccagtcc ctcatatctc tggacttgat tgaagatttt 8461 cttgaattag ctagtaggga gaagacagaa gataaagata aaccccttat ttataaaggt 8521 gaggggaagt ggcttcgaaa cattgactat taccgtttag atggttccac tactgcacag 8581 tcaaggaaga agtgggctga agaatttaat gatgaaacta atgtgagagg acgattattt 8641 atcatttcta ctaaagcagg atctctagga attaatctgg tagctgctaa tcgagtaatt 8701 atattcgacg cttcttggaa tccatcttat gacatccaga gtatattcag agtttatcgc 8761 tttggacaaa ctaagcctgt ttatgtatat aggttcttag ctcagggaac catggaagat 8821 aagatttatg atcggcaagt aactaagcag tcactgtctt ttcgagttgt tgatcagcag 8881 caggtggagc gtcattttac tatgaatgag cttactgaac tttatacttt tgagccagac 8941 ttattagatg accctaattc agaaaagaag aagaagaggg atactcccat gctgccaaag 9001 gataccatac ttgcagagct ccttcagata cataaagaac acattgtagg ataccatgaa 9061 catgattctc ttttggacca caaagaagaa gaagagttga ctgaagaaga aagaaaagca 9121 gcttgggctg agtatgaagc agagaagaag ggactgacca tgcgtttcaa cataccaact 9181 gggaccaatt taccccctgt cagtttcaac tctcaaactc cttatattcc tttcaatttg 9241 ggagccctgt cagcaatgag taatcaacag ctggaggacc tcattaatca aggaagagaa 9301 aaagttgtag aagcaacaaa cagtgtgaca gcagtgagga ttcaacctct tgaggatata 9361 atttcagctg tatggaagga gaacatgaat ctctcagagg cccaagtaca ggcgttagca 9421 ttaagtagac aagccagcca ggagcttgat gttaaacgaa gagaagcaat ctacaatgat 9481 gtattgacaa aacaacagat gttaatcagc tgtgttcagc gaatacttat gaacagaagg 9541 ctccagcagc agtacaatca gcagcaacag caacaaatga cttatcaaca agcaacactg 9601 ggtcacctca tgatgccaaa gcccccaaat ttgatcatga atccttctaa ctaccagcag 9661 attgatatga gaggaatgta tcagccagtg gctggtggta tgcagccacc accattacag 9721 cgtgcaccac ccccaatgag aagcaaaaat ccaggacctt cccaagggaa atcaatgGGA 9781 TCCGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA CAAGGATGAC 9841 GATGACAAGT AGCGGCCGCG AAGGATCTGC GATCGCTCCG GTGCCCGTCA GTGGGCAGAG 9901 CGCACATCGC CCACAGTCCC CGAGAAGTTG GGGGGAGGGG TCGGCAATTG AACGGGTGCC 9961 TAGAGAAGGT GGCGCGGGGT AAACTGGGAA AGTGATGTCG TGTACTGGCT CCGCCTTTTT 10021 CCCGAGGGTG GGGGAGAACC GTATATAAGT GCAGTAGTCG CCGTGAACGT TCTTTTTCGC 10081 AACGGGTTTG CCGCCAGAAC ACAGCTGAAG CTTCGAGGGG CTCGCATCTC TCCTTCACGC 10141 GCCCGCCGCC CTACCTGAGG CCGCCATCCA CGCCGGTTGA GTCGCGTTCT GCCGCCTCCC 10201 GCCTGTGGTG CCTCCTGAAC TGCGTCCGCC GTCTAGGTAA GTTTAAAGCT CAGGTCGAGA 10261 CCGGGCCTTT GTCCGGCGCT CCCTTGGAGC CTACCTAGAC TCAGCCGGCT CTCCACGCTT 10321 TGCCTGACCC TGCTTGCTCA ACTCTACGTC TTTGTTTCGT TTTCTGTTCT GCGCCGTTAC 10381 AGATCCAAGC TGTGACCGGC GCCTACGCTA GATGACCGAG TACAAGCCCA CGGTGCGCCT 10441 CGCCACCCGC GACGACGTCC CCCGGGCCGT ACGCACCCTC GCCGCCGCGT TCGCCGACTA 10501 CCCCGCCACG CGCCACACCG TCGACCCGGA CCGCCACATC GAGCGGGTCA CCGAGCTGCA 10561 AGAACTCTTC CTCACGCGCG TCGGGCTCGA CATCGGCAAG GTGTGGGTCG CGGACGACGG 10621 CGCCGCGGTG GCGGTCTGGA CCACGCCGGA GAGCGTCGAA GCGGGGGCGG TGTTCGCCGA 10681 GATCGGCCCG CGCATGGCCG AGTTGAGCGG TTCCCGGCTG GCCGCGCAGC AACAGATGGA 10741 AGGCCTCCTG GCGCCGCACC GGCCCAAGGA GCCCGCGTGG TTCCTGGCCA CCGTCGGCGT 10801 CTCGCCCGAC CACCAGGGCA AGGGTCTGGG CAGCGCCGTC GTGCTCCCCG GAGTGGAGGC 10861 GGCCGAGCGC GCCGGGGTGC CCGCCTTCCT GGAGACCTCC GCGCCCCGCA ACCTCCCCTT 10921 CTACGAGCGG CTCGGCTTCA CCGTCACCGC CGACGTCGAG GTGCCCGAAG GACCGCGCAC 10981 CTGGTGCATG ACCCGCAAGC CCGGTGCCTG AGTCGACAAT CAACCTCTGG ATTACAAAAT 11041 TTGTGAAAGA TTGACTGGTA TTCTTAACTA TGTTGCTCCT TTTACGCTAT GTGGATACGC 11101 TGCTTTAATG CCTTTGTATC ATGCTATTGC TTCCCGTATG GCTTTCATTT TCTCCTCCTT 11161 GTATAAATCC TGGTTGCTGT CTCTTTATGA GGAGTTGTGG CCCGTTGTCA GGCAACGTGG 11221 CGTGGTGTGC ACTGTGTTTG CTGACGCAAC CCCCACTGGT TGGGGCATTG CCACCACCTG 11281 TCAGCTCCTT TCCGGGACTT TCGCTTTCCC CCTCCCTATT GCCACGGCGG AACTCATCGC 11341 CGCCTGCCTT GCCCGCTGCT GGACAGGGGC TCGGCTGTTG GGCACTGACA ATTCCGTGGT 11401 GTTGTCGGGG AAATCATCGT CCTTTCCTTG GCTGCTCGCC TGTGTTGCCA CCTGGATTCT 11461 GCGCGGGACG TCCTTCTGCT ACGTCCCTTC GGCCCTCAAT CCAGCGGACC TTCCTTCCCG 11521 CGGCCTGCTG CCGGCTCTGC GGCCTCTTCC GCGTCTTCGC CTTCGCCCTC AGACGAGTCG 11581 GATCTCCCTT TGGGCCGCCT CCCCGCCTGG TACCTTTAAG ACCAATGACT TACAAGGCAG 11641 CTGTAGATCT TAGCCACTTT TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC 11701 AACGAAAATA AGATCTGCTT TTTGCTTGTA CTGGGTCTCT CTGGTTAGAC CAGATCTGAG 11761 CCTGGGAGCT CTCTGGCTAA CTAGGGAACC CACTGCTTAA GCCTCAATAA AGCTTGCCTT 11821 GAGTGCTTCA AGTAGTGTGT GCCCGTCTGT TGTGTGACTC TGGTAACTAG AGATCCCTCA 11881 GACCCTTTTA GTCAGTGTGG AAAATCTCTA GCAGTAGTAG TTCATGTCAT CTTATTATTC 11941 AGTATTTATA ACTTGCAAAG AAATGAATAT CAGAGAGTGA GAGGAACTTG TTTATTGCAG 12001 CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA GCATTTTTTT 12061 CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT GTCTGGCTCT 12121 AGCTATCCCG CCCCTAACTC CGCCCAGTTC CGCCCATTCT CCGCCCCATG GCTGACTAAT 12181 TTTTTTTATT TATGCAGAGG CCGAGGCCGC CTCGGCCTCT GAGCTATTCC AGAAGTAGTG 12241 AGGAGGCTTT TTTGGAGGCC TAGACTTTTG CAGAGACGGC CCAAATTCGT AATCATGGTC 12301 ATAGCTGTTT CCTGTGTGAA ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG 12361 AAGCATAAAG TGTAAAGCCT GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT 12421 GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG 12481 CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCTCT TCCGCTTCCT CGCTCACTGA 12541 CTCGCTGCGC TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 12601 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 12661 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 12721 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 12781 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 12841 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 12901 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 12961 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 13021 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 13081 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 13141 GACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 13201 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 13261 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 13321 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 13381 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 13441 GTAAACTTGG TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG 13501 TCTATTTCGT TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA 13561 GGGCTTACCA TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC 13621 AGATTTATCA GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC 13681 TTTATCCGCC TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC 13741 AGTTAATAGT TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC 13801 GTTTGGTATG GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC 13861 CATGTTGTGC AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT 13921 GGCCGCAGTG TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC 13981 ATCCGTAAGA TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG 14041 TATGCGGCGA CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG 14101 CAGAACTTTA AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT 14161 CTTACCGCTG TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC 14221 ATCTTTTACT TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA 14281 AAAGGGAATA AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATATTA 14341 TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA 14401 AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGTCTAAGA 14461 AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC CCTTTCGTCT 14521 CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC 14581 AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT 14641 TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA 14701 CCATATGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGCCA 14761 TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT 14821 ACGCCAGCTG GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT 14881 TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGCCAAG CTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.