pLV3-EF1a-CDK1-PGK-CopGFP-Puro

pLV3-EF1a-CDK1-PGK-CopGFP-Puro plasmid

PVT94359

pLV3-EF1a-CDK1-PGK-CopGFP-Puro plasmid Information

Plasmid name: pLV3-EF1a-CDK1(human)-PGK-CopGFP-Puro
Alias: NM_001786.5;CDC2; CDC28A; P34CDC2;
Gene lenght: 894bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: M13F-72
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-CDK1-PGK-CopGFP-Puro plasmid Description

pLV3-EF1a-CDK1-PGK-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-EF1a-CDK1-PGK-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 2461 gaccggcgcc tactctagag ctagcgaatt cGCCACCatg gaagattata ccaaaataga 2521 gaaaattgga gaaggtacct atggagttgt gtataagggt agacacaaaa ctacaggtca 2581 agtggtagcc atgaaaaaaa tcagactaga aagtgaagag gaaggggttc ctagtactgc 2641 aattcgggaa atttctctat taaaggaact tcgtcatcca aatatagtca gtcttcagga 2701 tgtgcttatg caggattcca ggttatatct catctttgag tttctttcca tggatctgaa 2761 gaaatacttg gattctatcc ctcctggtca gtacatggat tcttcacttg ttaagagtta 2821 tttataccaa atcctacagg ggattgtgtt ttgtcactct agaagagttc ttcacagaga 2881 cttaaaacct caaaatctct tgattgatga caaaggaaca attaaactgg ctgattttgg 2941 ccttgccaga gcttttggaa tacctatcag agtatataca catgaggtag taacactctg 3001 gtacagatct ccagaagtat tgctggggtc agctcgttac tcaactccag ttgacatttg 3061 gagtataggc accatatttg ctgaactagc aactaagaaa ccacttttcc atggggattc 3121 agaaattgat caactcttca ggattttcag agctttgggc actcccaata atgaagtgtg 3181 gccagaagtg gaatctttac aggactataa gaatacattt cccaaatgga aaccaggaag 3241 cctagcatcc catgtcaaaa acttggatga aaatggcttg gatttgctct cgaaaatgtt 3301 aatctatgat ccagccaaac gaatttctgg caaaatggca ctgaatcatc catattttaa 3361 tgatttggac aatcagatta agaagatgta ggcggccgct gggtagggga ggcgcttttc 3421 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt 3481 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt 3541 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg 3601 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag 3661 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag 3721 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc 3781 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc 3841 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg 3901 acctgcagcc caagcttacc atggagagcg acgagagcgg cctgcccgcc atggagatcg 3961 agtgccgcat caccggcacc ctgaacggcg tggagttcga gctggtgggc ggcggagagg 4021 gcacccccaa gcagggccgc atgaccaaca agatgaagag caccaaaggc gccctgacct 4081 tcagccccta cctgctgagc cacgtgatgg gctacggctt ctaccacttc ggcacctacc 4141 ccagcggcta cgagaacccc ttcctgcacg ccatcaacaa cggcggctac accaacaccc 4201 gcatcgagaa gtacgaggac ggcggcgtgc tgcacgtgag cttcagctac cgctacgagg 4261 ccggccgcgt gatcggcgac ttcaaggtgg tgggcaccgg cttccccgag gacagcgtga 4321 tcttcaccga caagatcatc cgcagcaacg ccaccgtgga gcacctgcac cccatgggcg 4381 ataacgtgct ggtgggcagc ttcgcccgca ccttcagcct gcgcgacggc ggctactaca 4441 gcttcgtggt ggacagccac atgcacttca agagcgccat ccaccccagc atcctgcaga 4501 acgggggccc catgttcgcc ttccgccgcg tggaggagct gcacagcaac accgagctgg 4561 gcatcgtgga gtaccagcac gccttcaaga cccccatcgc cttcgccaga tcccgcgctc 4621 agtcgtccaa ttctgccgtg gacggcaccg ccggacccgg ctccaccgga tctcgcgagg 4681 gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct atgaccgagt 4741 acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg 4801 ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg 4861 agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg 4921 tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag agcgtcgaag 4981 cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg 5041 ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt 5101 tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg 5161 tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg 5221 cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg 5281 tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga gtcgacaatc 5341 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt 5401 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg 5461 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc 5521 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt 5581 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg 5641 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg 5701 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct 5761 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc 5821 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc 5881 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggt acctttaaga 5941 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 6001 gaagggctaa ttcactccca acgaaaataa gatctgcttt ttgcttgtac tgggtctctc 6061 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 6121 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 6181 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 6241 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 6301 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 6361 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 6421 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc 6481 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 6541 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc 6601 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 6661 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 6721 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 6781 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 6841 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 6901 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 6961 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 7021 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 7081 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 7141 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 7201 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 7261 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 7321 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 7381 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 7441 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 7501 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 7561 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 7621 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 7681 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 7741 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 7801 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 7861 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 7921 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 7981 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 8041 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 8101 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 8161 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 8221 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 8281 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 8341 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 8401 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 8461 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 8521 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 8581 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 8641 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 8701 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 8761 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 8821 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 8881 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 8941 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 9001 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 9061 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 9121 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa 9181 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc 9241 tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.