1179_pAAV-U6-BbsI-gRNA-CB-EmGF

1179_pAAV-U6-BbsI-gRNA-CB-EmGFP plasmid

PVT94295

1179_pAAV-U6-BbsI-gRNA-CB-EmGFP plasmid Information

Plasmid name: 1179_pAAV-U6-BbsI-gRNA-CB-EmGFP
Alias: Plasmid#89060
Gene lenght:
Host: Mammalian cells,Adeno associated virus
Use(s): Gene editing
Fragment type: CRISPR
Species: Empty Vector
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: EGFP-F4
Reverse primer:
Copy number: High
Induction:

1179_pAAV-U6-BbsI-gRNA-CB-EmGFP plasmid Description

1179_pAAV-U6-BbsI-gRNA-CB-EmGFP is a plasmid suitable for use in mammalian cells, and can also be used for adeno-associated virus packaging.

1179_pAAV-U6-BbsI-gRNA-CB-EmGFP plasmid Sequence

1 cttcgctatt acgccagctg cgcgctcgct cgctcactga ggccgcccgg gcaaagcccg 61 ggcgtcgggc gacctttggt cgcccggcct cagtgagcga gcgagcgcgc agagagggag 121 tggccaactc catcactagg ggttccttgt agttaatgat taacccgcca tgctacttat 181 ctacgtagcc atgctctggt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac 241 tggatccggt accaaggtcg ggcaggaaga gggcctattt cccatgattc cttcatattt 301 gcatatacga tacaaggctg ttagagagat aattagaatt aatttgactg taaacacaaa 361 gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt ttgcagtttt 421 aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt atttcgattt 481 cttggcttta tatatcttgt ggaaaggacg aaacaccggg tcttcgagaa gacctgtttt 541 agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa aaagtggcac 601 cgagtcggtg ctttttttct agacccagct ttcaagcttg attaacccgc catgctactt 661 atctacgtag ccatgctcta ggaagatcgg aattcgccct taagctagta tgccaagtac 721 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac 781 cttatgggac tttcctactt ggcagtacat ctactcgagg ccacgttctg cttcactctc 841 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt 901 gcagcgatgg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc 961 agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata 1021 aaaagcgaag cgcgcggcgg gcgggagcgg gatcagaatg atctgatatc atcgatgaat 1081 tcgagctcac catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg 1141 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg 1201 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc 1261 cctggcccac cctcgtgacc accttcacct acggcgtgca gtgcttcgcc cgctaccccg 1321 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc 1381 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg 1441 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca 1501 tcctggggca caagctggag tacaactaca acagccacaa ggtctatatc accgccgaca 1561 agcagaagaa cggcatcaag gtgaacttca agacccgcca caacatcgag gacggcagcg 1621 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc 1681 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg 1741 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc 1801 tgtacaagta agaattctgc agtcgacgcg ggcccgggat ccaccggtgc ggccgctcgc 1861 gaataaaaga tctttatttt cattagatct gtgtgttggt tttttgtgtg atgcagctac 1921 gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg 1981 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga 2041 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cattaatgaa 2101 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 2161 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 2221 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 2281 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 2341 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 2401 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 2461 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 2521 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 2581 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 2641 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 2701 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 2761 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 2821 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 2881 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 2941 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 3001 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 3061 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 3121 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 3181 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 3241 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 3301 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 3361 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 3421 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 3481 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 3541 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 3601 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 3661 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 3721 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 3781 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 3841 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 3901 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 3961 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 4021 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt 4081 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa 4141 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt 4201 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa 4261 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag 4321 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt 4381 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg 4441 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc 4501 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg 4561 ggaagggcga tcggtgcggg cct

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.