pCMV-GBA2-FLAG-Neo plasmid

pCMV-GBA2-FLAG-Neo plasmid

PVT94572

pCMV-GBA2-FLAG-Neo plasmid Information

Plasmid name: pCMV-GBA2(human)-FLAG-Neo
Alias: NM_020944.2;AD035; NLGase; SPG46
Gene lenght: 2781bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-GBA2-FLAG-Neo plasmid Description

pCMV-GBA2-FLAG-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-GBA2-FLAG-Neo plasmid Sequence

1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCGGC GCGCCAGATC TCAAGCTTAA CTAGCTAGCG GACCGACGCG TACGCGGCCG 1081 CGCCACCatg gggacccagg atccagggaa catgggaacc ggcgtcccag cctcggagca 1141 gataagctgt gccaaagagg atccacaagt ttattgccct gaagagactg gcggcaccaa 1201 ggatgtgcag gttacagact gtaagagtcc cgaagacagc cgacccccaa aagagacgga 1261 ctgctgcaat ccggaggact ctgggcagct gatggtttcc tatgagggta aagctatggg 1321 ctaccaggtg cctccctttg gctggcgcat ctgtctggct catgagttta cagagaagag 1381 gaaacccttt caagctaaca acgtctccct aagcaacatg ataaagcata taggcatggg 1441 cttgaggtac ctgcagtggt ggtaccggaa gacccatgtg gaaaagaaga cacctttcat 1501 cgacatgatc aattctgtac ccctaagaca gatttatggt tgtcccttgg gtggcatcgg 1561 gggaggcact attacccgtg gctggagagg ccagttctgt cgttggcagc ttaaccctgg 1621 aatgtatcag caccggacag tcatcgctga ccaattcaca gtgtgcctgc gtcgggaagg 1681 gcagactgtg taccagcaag tcctgtccct ggagcgccca agtgtcctcc gcagctggaa 1741 ctggggcctg tgtgggtact ttgctttcta ccatgccctc tatccccgag cctggactgt 1801 ctatcagctt cctggccaga atgtcaccct cacctgccgt cagatcacac ccatcttgcc 1861 ccatgactac caggacagca gcctgcctgt aggagtcttt gtgtgggatg tggaaaatga 1921 aggggacgaa gctctagatg tgtccatcat gttctccatg cggaatggac tgggtggtgg 1981 agacgatgcc ccagggggtt tgtggaatga gcccttctgt ctggagcgta gcggggaaac 2041 tgtccggggg ctgctcctgc atcatccaac ccttccaaac ccctacacga tggctgtggc 2101 tgcacgagtc acggcagcta ccacggtaac ccacatcaca gcctttgacc ctgacagcac 2161 ggggcagcag gtgtggcagg atctacttca ggatggacag ctggactctc ccactggcca 2221 aagcacccct acgcagaaag gagtaggcat tgctggagct gtgtgtgttt ccagcaagtt 2281 gcgacctcga ggccagtgcc gcctggagtt ttcactggct tgggacatgc ccaggatcat 2341 gtttggagct aaaggccaag tccactacag gcggtataca aggttctttg gccaggatgg 2401 agatgcagca cctgccctca gccactatgc actgtgccga tacgcagagt gggaagagag 2461 gatctcagct tggcagagcc cggtattgga tgacagatca ctgcctgcct ggtacaaatc 2521 tgcgctgttc aatgaactat acttcctggc tgatggaggc acagtgtggc tggaagttct 2581 tgaggactcc ctaccagagg agctgggcag aaacatgtgt cacctccgcc ccaccctacg 2641 ggactacggt cgatttggct accttgaggg ccaggagtac cgcatgtaca acacatatga 2701 tgtccacttt tatgcttcct ttgccctcat catgctctgg cccaaacttg agctcagcct 2761 acagtatgac atggctctgg ccactctcag ggaggacctg acacggcgac ggtacctgat 2821 gagtggggtg atggcacctg tgaaaaggag gaacgtcatc ccccatgata ttggggaccc 2881 agatgatgaa ccatggctcc gcgtcaatgc atatttaatc catgatactg ctgattggaa 2941 ggacctgaac ctgaagtttg tgctgcaggt ttatcgggac tattacctca cgggtgatca 3001 aaacttcctg aaggacatgt ggcctgtgtg tctagctgtg atggaatctg aaatgaagtt 3061 tgacaaggac catgatggac tcattgaaaa tggaggctat gcagaccaga cctatgatgg 3121 atgggtgacc acaggcccca gtgcttactg tggagggctg tggctggcag ctgtggctgt 3181 gatggtccag atggctgctc tgtgtggggc acaggacatc caggataagt tttcttctat 3241 cctcagccgg ggccaagaag cctatgagag actgctgtgg aatggccgct attacaacta 3301 tgacagcagc tctcggcctc agtctcgtag tgttatgtct gaccagtgtg ctggacagtg 3361 gttcctgaag gcctgtggcc taggagaagg agacactgag gtgtttccta cccaacatgt 3421 ggtccgtgct ctccaaacta tctttgagct gaacgtccag gcctttgcag gaggggccat 3481 gggggctgtg aatgggatgc agccccatgg tgtccctgat aaatccagtg tgcagtctga 3541 tgaagtctgg gtgggtgtgg tctacgggct ggcagctacc atgatccaag agggcctgac 3601 ttgggagggc ttccagacag ctgaaggctg ctaccgtacc gtgtgggagc gcctgggtct 3661 ggccttccag accccagagg catactgcca gcagcgagtg ttccgctcac tggcctacat 3721 gcggccactg agcatatggg ccatgcagct agccctgcaa cagcagcagc acaaaaaggc 3781 ctcctggcca aaagtcaaac agggcacagg actaaggaca gggcctatgt ttggaccaaa 3841 ggaagccatg gcaaacctga gcccagagGA TATCCTGGAT TACAAGGATG ACGACGATAA 3901 GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT 3961 GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC 4021 TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA 4081 TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT 4141 GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT 4201 CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG 4261 CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT 4321 TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT 4381 TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC 4441 TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG 4501 GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC 4561 CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA 4621 CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA 4681 AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT 4741 GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA 4801 AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG 4861 CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA 4921 CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA 4981 CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG 5041 GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG 5101 TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA 5161 ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC 5221 TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG 5281 ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC 5341 GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC 5401 TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG 5461 TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA 5521 CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG 5581 GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA 5641 AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT 5701 TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC 5761 AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG 5821 CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC 5881 GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT 5941 AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG 6001 CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC 6061 GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA 6121 TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC 6181 TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT 6241 CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG 6301 CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG 6361 CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA 6421 CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC 6481 AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA 6541 GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA 6601 ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT 6661 CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC 6721 CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC 6781 TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT 6841 AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA 6901 GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT 6961 GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC 7021 ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC 7081 AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG 7141 GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG 7201 TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG 7261 CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG 7321 CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC 7381 TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA 7441 AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC 7501 CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC 7561 TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT 7621 GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.