pCMV-PGK1-EYFP-Neo plasmid

pCMV-PGK1-EYFP-Neo plasmid

PVT95735

pCMV-PGK1-EYFP-Neo plasmid Information

Plasmid name: pCMV-PGK1(human)-EYFP-Neo
Alias: NM_000291.4;HEL-S-68p; MIG10; PGKA
Gene lenght: 1251bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Yellow
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-PGK1-EYFP-Neo plasmid Description

pCMV-PGK1-EYFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-PGK1-EYFP-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaacgg gccctctaga ctcgagGCCA CCatgtcgct ttctaacaag ctgacgctgg 961 acaagctgga cgttaaaggg aagcgggtcg ttatgagagt cgacttcaat gttcctatga 1021 agaacaacca gataacaaac aaccagagga ttaaggctgc tgtcccaagc atcaaattct 1081 gcttggacaa tggagccaag tcggtagtcc ttatgagcca cctaggccgg cctgatggtg 1141 tgcccatgcc tgacaagtac tccttagagc cagttgctgt agaactcaaa tctctgctgg 1201 gcaaggatgt tctgttcttg aaggactgtg taggcccaga agtggagaaa gcctgtgcca 1261 acccagctgc tgggtctgtc atcctgctgg agaacctccg ctttcatgtg gaggaagaag 1321 ggaagggaaa agatgcttct gggaacaagg ttaaagccga gccagccaaa atagaagctt 1381 tccgagcttc actttccaag ctaggggatg tctatgtcaa tgatgctttt ggcactgctc 1441 acagagccca cagctccatg gtaggagtca atctgccaca gaaggctggt gggtttttga 1501 tgaagaagga gctgaactac tttgcaaagg ccttggagag cccagagcga cccttcctgg 1561 ccatcctggg cggagctaaa gttgcagaca agatccagct catcaataat atgctggaca 1621 aagtcaatga gatgattatt ggtggtggaa tggcttttac cttccttaag gtgctcaaca 1681 acatggagat tggcacttct ctgtttgatg aagagggagc caagattgtc aaagacctaa 1741 tgtccaaagc tgagaagaat ggtgtgaaga ttaccttgcc tgttgacttt gtcactgctg 1801 acaagtttga tgagaatgcc aagactggcc aagccactgt ggcttctggc atacctgctg 1861 gctggatggg cttggactgt ggtcctgaaa gcagcaagaa gtatgctgag gctgtcactc 1921 gggctaagca gattgtgtgg aatggtcctg tgggggtatt tgaatgggaa gcttttgccc 1981 ggggaaccaa agctctcatg gatgaggtgg tgaaagccac ttctaggggc tgcatcacca 2041 tcataggtgg tggagacact gccacttgct gtgccaaatg gaacacggag gataaagtca 2101 gccatgtgag cactgggggt ggtgccagtt tggagctcct ggaaggtaaa gtccttcctg 2161 gggtggatgc tctcagcaat attggtggag gcggatcagg aggcggtgga tctgatatca 2221 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg 2281 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg 2341 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc 2401 tcgtgaccac cttcggctac ggcctgcagt gcttcgcccg ctaccccgac cacatgaagc 2461 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct 2521 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg 2581 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca 2641 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg 2701 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg 2761 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact 2821 acctgagcta ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc 2881 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagtaag 2941 agctcggtac caagcttggg cccgaacaaa aactcatctc agaagaggat ctgaatagcg 3001 ccgtcgacca tcatcatcat catcattgag tttaaacggt ctccagctta agtttaaacc 3061 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg 3121 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa 3181 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca 3241 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg 3301 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg 3361 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg 3421 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc 3481 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc 3541 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga 3601 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa 3661 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga 3721 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct 3781 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat 3841 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc 3901 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac 3961 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact 4021 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta 4081 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc 4141 cattttcgga tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg 4201 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca 4261 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt 4321 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg 4381 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga 4441 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca 4501 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct 4561 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac 4621 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc 4681 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt 4741 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt 4801 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg 4861 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat 4921 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc 4981 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc 5041 gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc 5101 tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt 5161 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca 5221 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc 5281 tgtataccgt cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg 5341 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 5401 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 5461 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 5521 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 5581 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 5641 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 5701 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 5761 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 5821 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 5881 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 5941 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 6001 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 6061 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 6121 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 6181 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 6241 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 6301 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 6361 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 6421 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 6481 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 6541 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 6601 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 6661 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 6721 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 6781 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 6841 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 6901 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 6961 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 7021 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 7081 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 7141 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 7201 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 7261 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 7321 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 7381 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 7441 gttccgcgca catttccccg aaaagtgcca cctgacgtc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.