pEF1a-EGFP-P2A-Zfp467-2-HA-Neo

pEF1a-EGFP-P2A-Zfp467-2-HA-Neo plasmid

PVT94212

pEF1a-EGFP-P2A-Zfp467-2-HA-Neo plasmid Information

Plasmid name: pEF1a-EGFP-P2A-Zfp467(mouse)-2-HA-Neo
Alias: NM_001085417.1;1190001I08Rik; EZI; MNCb-3350; Znf467
Gene lenght: 339bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: EF1a
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: BGH-R
Copy number: High
Induction:

pEF1a-EGFP-P2A-Zfp467-2-HA-Neo plasmid Description

pEF1a-EGFP-P2A-Zfp467-2-HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pEF1a-EGFP-P2A-Zfp467-2-HA-Neo plasmid Sequence

1 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc 61 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc 121 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc 181 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc 241 ggcatggacg agctgtacaa gGGATCCGGA GCTACTAACT TCAGCCTGCT GAAGCAGGCT 301 GGCGACGTGG AGGAGAACCC TGGACCTTCG atgagagaga ccctggaggc cctgaactcc 361 ttgggattct cagtgggaca gccagagatg gctccccaga gtgagcccag ggatgggttc 421 agtaatgccc aggagaagat gtcatccaga ggagagagca cactgcactc ctgctcaggg 481 catgagactc ctggccagaa agaaggcatc cacacagaac aagctgaagc tccttgtatg 541 ggcagccagg ccagtacccc acagaaggcc gaacctgcag gctctgtccc aggagaccac 601 atgaagttcc actgttctct gaggaggaaa catctcatgt ctcctgatgc ctgctcttca 661 gtttctgtcT ACCCATACGA CGTACCAGAT TACGCTTGAC GAGAATTCTG CAGATATCCA 721 GCACAGTGGC GGCCGCTCGA GTCTAGAGGG CCCTTCGAAC AAAAACTCAT CTCAGAAGAG 781 GATCTGAATA TGCATACCGG TCATCATCAC CATCACCATT GAGTTTAAAC CCGCTGATCA 841 GCCTCGACTG TGCCTTCTAG TTGCCAGCCA TCTGTTGTTT GCCCCTCCCC CGTGCCTTCC 901 TTGACCCTGG AAGGTGCCAC TCCCACTGTC CTTTCCTAAT AAAATGAGGA AATTGCATCG 961 CATTGTCTGA GTAGGTGTCA TTCTATTCTG GGGGGTGGGG TGGGGCAGGA CAGCAAGGGG 1021 GAGGATTGGG AAGACAATAG CAGGCATGCT GGGGATGCGG TGGGCTCTAT GGCTTCTGAG 1081 GCGGAAAGAA aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc 1141 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 1201 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 1261 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 1321 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 1381 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctcc 1441 cgggagcttg gatatccatt ttcggatctg atcaagagac aggatgagga tcgtttcgca 1501 tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg 1561 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag 1621 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc 1681 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc 1741 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg 1801 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc 1861 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca 1921 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag 1981 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg 2041 gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg 2101 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca 2161 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc 2221 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg 2281 acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga cgcccaacct 2341 gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct tcggaatcgt 2401 tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg agttcttcgc 2461 ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 2521 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 2581 tgtatcttat catgtctgga tcccttgaaa tacatccaca ggtacacctc caattgactc 2641 aaatgatgtc aattagtcta tcagaagctt gtggaaggct actcgaaatg tttgacccaa 2701 gttaaacaat ttaaaggcaa tgctaccaaa tactaattga gtgtatgtaa acttctgacc 2761 cactgggaat gtgatgaaag aaataaaagc tgaaatgaat cattctctct actattattc 2821 tgatatttca cattcttaaa ataaagtggt gatcctaact gacctaagac agggaatttt 2881 tactaggatt aaatgtcagg aattgtgaaa aagtgagttt aaatgtattt ggctaaggtg 2941 tatgtaaact tccgacttca actgtatagg gatcctctag ctagagtcga cctcgagggg 3001 gggcccggta cccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc 3061 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 3121 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 3181 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 3241 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 3301 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 3361 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 3421 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 3481 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 3541 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 3601 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 3661 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 3721 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 3781 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 3841 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 3901 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 3961 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 4021 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 4081 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 4141 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 4201 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 4261 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 4321 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 4381 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 4441 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 4501 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 4561 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 4621 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 4681 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 4741 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 4801 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 4861 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 4921 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 4981 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 5041 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 5101 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 5161 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgc 5221 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 5281 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 5341 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 5401 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 5461 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 5521 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 5581 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 5641 gaattttaac aaaatattaa cgcttacaat ttccattcgc cattcaggct gcgcaactgt 5701 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt 5761 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg 5821 acggccagtg agcgcgcgta atacgactca ctatagggcg aattggagct cggatcccta 5881 tacagttgaa gtcggaagtt tacatacact taagttggag tcattaaaac tcgtttttca 5941 actactccac aaatttcttg ttaacaaaca atagttttgg caagtcagtt aggacatcta 6001 ctttgtgcat gacacaagtc atttttccaa caattgttta cagacagatt atttcactta 6061 taattcactg tatcacaatt ccagtgggtc agaagtttac atacactaag ttgactgtgc 6121 ctttaaacag cttggaaaat tccagaaaat gatgtcatgg ctttagaagc ttcccacaat 6181 aagttgggtg aattttggct cattcctcct ttctatagga ttgaggtcag agctttgtga 6241 tggCTAGGTC TTGAAAGGAG TGGGAATTGG CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC 6301 ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGAACCG GTGCCTAGAG 6361 AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA 6421 GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT TTCGCAACGG 6481 GTTTGCCGCC AGAACACAGG TAAGTGCCGT GTGTGGTTCC CGCGGGCCTG GCCTCTTTAC 6541 GGGTTATGGC CCTTGCGTGC CTTGAATTAC TTCCACCTGG CTGCAGTACG TGATTCTTGA 6601 TCCCGAGCTT CGGGTTGGAA GTGGGTGGGA GAGTTCGAGG CCTTGCGCTT AAGGAGCCCC 6661 TTCGCCTCGT GCTTGAGTTG AGGCCTGGCC TGGGCGCTGG GGCCGCCGCG TGCGAATCTG 6721 GTGGCACCTT CGCGCCTGTC TCGCTGCTTT CGATAAGTCT CTAGCCATTT AAAATTTTTG 6781 ATGACCTGCT GCGACGCTTT TTTTCTGGCA AGATAGTCTT GTAAATGCGG GCCAAGATCT 6841 GCACACTGGT ATTTCGGTTT TTGGGGCCGC GGGCGGCGAC GGGGCCCGTG CGTCCCAGCG 6901 CACATGTTCG GCGAGGCGGG GCCTGCGAGC GCGGCCACCG AGAATCGGAC GGGGGTAGTC 6961 TCAAGCTGGC CGGCCTGCTC TGGTGCCTGG CCTCGCGCCG CCGTGTATCG CCCCGCCCTG 7021 GGCGGCAAGG CTGGCCCGGT CGGCACCAGT TGCGTGAGCG GAAAGATGGC CGCTTCCCGG 7081 CCCTGCTGCA GGGAGCTCAA AATGGAGGAC GCGGCGCTCG GGAGAGCGGG CGGGTGAGTC 7141 ACCCACACAA AGGAAAAGGG CCTTTCCGTC CTCAGCCGTC GCTTCATGTG ACTCCACGGA 7201 GTACCGGGCG CCGTCCAGGC ACCTCGATTA GTTCTCGAGC TTTTGGAGTA CGTCGTCTTT 7261 AGGTTGGGGG GAGGGGTTTT ATGCGATGGA GTTTCCCCAC ACTGAGTGGG TGGAGACTGA 7321 AGTTAGGCCA GCTTGGCACT TGATGTAATT CTCCTTGGAA TTTGCCCTTT TTGAGTTTGG 7381 ATCTTGGTTC ATTCTCAAGC CTCAGACAGT GGTTCAAAGT TTTTTTCTTC CATTTCAGGT 7441 GTCGTGAGGA ATTAGCTTGG TACTAATACG ACTCACTATA GGGAGACCCA AGCTGGCTAG 7501 GTAAGCTTGG TACCGAGCTC GGATCCACTA GTCCAGTGTG GTGGtaccct cgtaaagtcg 7561 acccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga 7621 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc 7681 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg 7741 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca 7801 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac 7861 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga 7921 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct 7981 ggggcacaag ctggagtaca actacaacag ccacaacgtc tat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.