pLV3-U6-DUXAP8-lncRNA-shRNA2-C

pLV3-U6-DUXAP8-lncRNA-shRNA2-CopGFP-Puro plasmid

PVT94209

pLV3-U6-DUXAP8-lncRNA-shRNA2-CopGFP-Puro plasmid Information

Plasmid name: pLV3-U6-DUXAP8(human)-lncRNA-shRNA2-CopGFP-Puro
Alias: CAGCATACTTCAAATTCACAGCAAAT
Gene lenght:
Host: Mammalian cells,lentivirus
Use(s): Interference silence
Fragment type: shRNA
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: U6
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: U6-F
Reverse primer:
Copy number: High
Induction:

pLV3-U6-DUXAP8-lncRNA-shRNA2-CopGFP-Puro plasmid Description

pLV3-U6-DUXAP8-lncRNA-shRNA2-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-U6-DUXAP8-lncRNA-shRNA2-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct 241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa 301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc 361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc 421 ccgaacaggg acctgaaagc gaaagggaaa ccagagctct ctcgacgcag gactcggctt 481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg 541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga 601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta 661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta 721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga 781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg 841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt 901 aagaccaccg cacagcaagc ggccactgat cttcagacct ggaggaggag atatgaggga 961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc 1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc 1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcct caatgacgct 1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag 1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca 1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg 1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa 1381 atctctggaa cagattggaa tcacacgacc tggatggagt gggacagaga aattaacaat 1441 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa 1501 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat 1561 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata 1621 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt 1681 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt 1741 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtATCGGTta 1801 acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata 1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaatt caaaatttta 1921 tcgatactag tGAGGGCCTA TTTCCCATGA TTCCTTCATA TTTGCATATA CGATACAAGG 1981 CTGTTAGAGA GATAATTAGA ATTAATTTGA CTGTAAACAC AAAGATATTA GTACAAAATA 2041 CGTGACGTAG AAAGTAATAA TTTCTTGGGT AGTTTGCAGT TTTAAAATTA TGTTTTAAAA 2101 TGGACTATCA TATGCTTACC GTAACTTGAA AGTATTTCGA TTTCTTGGCT TTATATATCT 2161 TGTGGAAAGG ACTCATCgct agcgaattcg CAGCATACTT CAAATTCACA GCAAATctcg 2221 agATTTGCTG TGAATTTGAA GTATGCTGtt ttttggatcc gcggccgcGa aggatctgcg 2281 atcgctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg 2341 ggggaggggt cggcaattga acgggtgcct agagaaggtg gcgcggggta aactgggaaa 2401 gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg 2461 cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca cagctgaagc 2521 ttcgaggggc tcgcatctct ccttcacgcg cccgccgccc tacctgaggc cgccatccac 2581 gccggttgag tcgcgttctg ccgcctcccg cctgtggtgc ctcctgaact gcgtccgccg 2641 tctaggtaag tttaaagctc aggtcgagac cgggcctttg tccggcgctc ccttggagcc 2701 tacctagact cagccggctc tccacgcttt gcctgaccct gcttgctcaa ctctacgtct 2761 ttgtttcgtt ttctgttctg cgccgttaca gatccaagct gtgaccggcg cctacgctag 2821 acgccaccat ggagagcgac gagagcggcc tgcccgccat ggagatcgag tgccgcatca 2881 ccggcaccct gaacggcgtg gagttcgagc tggtgggcgg cggagagggc acccccaagc 2941 agggccgcat gaccaacaag atgaagagca ccaaaggcgc cctgaccttc agcccctacc 3001 tgctgagcca cgtgatgggc tacggcttct accacttcgg cacctacccc agcggctacg 3061 agaacccctt cctgcacgcc atcaacaacg gcggctacac caacacccgc atcgagaagt 3121 acgaggacgg cggcgtgctg cacgtgagct tcagctaccg ctacgaggcc ggccgcgtga 3181 tcggcgactt caaggtggtg ggcaccggct tccccgagga cagcgtgatc ttcaccgaca 3241 agatcatccg cagcaacgcc accgtggagc acctgcaccc catgggcgat aacgtgctgg 3301 tgggcagctt cgcccgcacc ttcagcctgc gcgacggcgg ctactacagc ttcgtggtgg 3361 acagccacat gcacttcaag agcgccatcc accccagcat cctgcagaac gggggcccca 3421 tgttcgcctt ccgccgcgtg gaggagctgc acagcaacac cgagctgggc atcgtggagt 3481 accagcacgc cttcaagacc cccatcgcct tcgccagatc ccgcgctcag tcgtccaatt 3541 ctgccgtgga cggcaccgcc ggacccggct ccaccggatc tcgcgagggc agaggaagtc 3601 ttctaacatg cggtgacgtg gaggagaatc ccggccctat gaccgagtac aagcccacgg 3661 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg 3721 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg 3781 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg 3841 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt 3901 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac 3961 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg 4021 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag 4081 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc 4141 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac 4201 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgaaa tcaacctctg gattacaaaa 4261 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg 4321 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct 4381 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg 4441 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct 4501 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg 4561 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg 4621 tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc 4681 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc 4741 gcggcctgct gccggctctg cggcctcttc cgcgtctccg ccttcgccct cagacgagtc 4801 ggatctccct ttggccgcct ccccgcctgg tacctttaag accaatgact tacaaggcag 4861 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc 4921 aacgaaaata agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag 4981 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt 5041 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca 5101 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc 5161 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag 5221 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 5281 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct 5341 agctatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat 5401 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg 5461 aggaggcttt tttggaggcc tagacttttg cagagacggc ccaaattcgt aatcatggtc 5521 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg 5581 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt 5641 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 5701 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 5761 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 5821 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 5881 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 5941 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 6001 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 6061 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 6121 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 6181 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 6241 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 6301 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 6361 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 6421 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 6481 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 6541 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 6601 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 6661 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 6721 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 6781 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 6841 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 6901 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 6961 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 7021 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 7081 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 7141 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 7201 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 7261 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 7321 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 7381 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 7441 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 7501 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 7561 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 7621 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga 7681 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct 7741 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 7801 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 7861 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca 7921 ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgcca 7981 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt 8041 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt 8101 ttcccagtca cgacgttgta aaacgacggc cagtgccaag ctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.