pCMV-DDX21-EGFP-Neo plasmid

pCMV-DDX21-EGFP-Neo plasmid

PVT94140

pCMV-DDX21-EGFP-Neo plasmid Information

Plasmid name: pCMV-DDX21(human)-EGFP-Neo
Alias: NM_004728.4;gu-alpha; GUA; GURDB; II/Gu; RH; RH II/Gu;RH-II/GU; RH-II/GuA
Gene lenght: 2349bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-DDX21-EGFP-Neo plasmid Description

pCMV-DDX21-EGFP-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-DDX21-EGFP-Neo plasmid Sequence

1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc 901 gtttaaagcc actgggatcc GCCACCatgc cgggaaaact ccgtagtgac gctggtttgg 961 aatcagacac cgcaatgaaa aaaggggaga cactgcgaaa gcaaaccgag gagaaagaga 1021 aaaaagagaa gccaaaatct gataagactg aagagatagc agaagaggaa gaaactgttt 1081 tccccaaagc taaacaagtt aaaaagaaag cagagccttc tgaagttgac atgaattctc 1141 ctaaatccaa aaaggcaaaa aagaaagagg agccatctca aaatgacatt tctcctaaaa 1201 ccaaaagttt gagaaagaaa aaggagccca ttgaaaagaa agtggtttct tctaaaacca 1261 aaaaagtgac aaaaaatgag gagccttctg aggaagaaat agatgctcct aagcccaaga 1321 agatgaagaa agaaaaggaa atgaatggag aaactagaga gaaaagcccc aaactgaaga 1381 atggatttcc tcatcctgaa ccggactgta accccagtga agctgccagt gaagaaagta 1441 acagtgagat agagcaggaa atacctgtgg aacaaaaaga aggcgctttc tctaattttc 1501 ccatatctga agaaactatt aaacttctca aaggccgagg agtgaccttc ctatttccta 1561 tacaagcaaa gacattccat catgtttaca gcgggaagga cttaattgca caggcacgga 1621 caggaactgg gaagacattc tcctttgcca tccctttgat tgagaaactt catggggaac 1681 tgcaagacag gaagagaggc cgtgcccctc aggtactggt tcttgcacct acaagagagt 1741 tggcaaatca agtaagcaaa gacttcagtg acatcacaaa aaagctgtca gtggcttgtt 1801 tttatggtgg aactccctat ggaggtcaat ttgaacgcat gaggaatggg attgatatcc 1861 tggttggaac accaggtcgt atcaaagacc acatacagaa tggcaaacta gatctcacca 1921 aacttaagca tgttgtcctg gatgaagtgg accagatgtt ggatatggga tttgctgatc 1981 aagtggaaga gattttaagt gtggcataca agaaagattc tgaagacaat ccccaaacat 2041 tgcttttttc tgcaacttgc cctcattggg tatttaatgt tgccaagaaa tacatgaaat 2101 ctacatatga acaggtggac ctgattggta aaaagactca gaaaacggca ataactgtgg 2161 agcatctggc tattaagtgc cactggactc agagggcagc agttattggg gatgtcatcc 2221 gagtatatag tggtcatcaa ggacgcacta tcatcttttg tgaaaccaag aaagaagccc 2281 aggagctgtc ccagaattca gctataaagc aggatgctca gtccttgcat ggagacattc 2341 cacagaagca aagggaaatc accctgaaag gttttagaaa tggtagtttt ggagttttgg 2401 tggcaaccaa tgttgctgca cgtgggttag acatccctga ggttgatttg gttatacaaa 2461 gctctccacc aaaggatgta gagtcctaca ttcatcgatc cgggcggaca ggcagagctg 2521 gaaggacggg ggtgtgcatc tgcttttatc agcacaagga agaatatcag ttagtacaag 2581 tggagcaaaa agcgggaatt aagttcaaac gaataggtgt tccttctgca acagaaataa 2641 taaaagcttc cagcaaagat gccatcaggc ttttggattc cgtgcctccc actgccatta 2701 gtcacttcaa acaatcagct gagaagctga tagaggagaa gggagctgtg gaagctctgg 2761 cagcagcact ggcccatatt tcaggtgcca cgtccgtaga ccagcgctcc ttgatcaact 2821 caaatgtggg ttttgtgacc atgatcttgc agtgctcaat tgaaatgcca aatattagtt 2881 atgcttggaa agaacttaaa gagcagctgg gcgaggagat tgattccaaa gtgaagggaa 2941 tggtttttct caaaggaaag ctgggtgttt gctttgatgt acctaccgca tcagtaacag 3001 aaatacagga gaaatggcat gattcacgac gctggcagct ctctgtggcc acagagcaac 3061 cagaactgga aggaccacgg gaaggatatg gaggcttcag gggacagcgg gaaggcagtc 3121 gaggcttcag gggacagcgg gacggaaaca gaagattcag aggacagcgg gaaggcagta 3181 gaggcccgag aggacagcga tcaggaggtg gcaacaaaag taacagatcc caaaacaaag 3241 gccagaagcg gagtttcagt aaagcatttg gtcaaCCctc gagtggcggt ggaggaagcg 3301 gcggaggtgg atctatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc 3361 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg 3421 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg 3481 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc 3541 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg 3601 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg 3661 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca 3721 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg 3781 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca 3841 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc 3901 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc 3961 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg 4021 agctgtacaa gtagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt 4081 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg 4141 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt 4201 gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca 4261 atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct 4321 ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg 4381 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt 4441 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc 4501 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg 4561 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg 4621 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct 4681 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg 4741 agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg 4801 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 4861 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 4921 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 4981 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 5041 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 5101 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac 5161 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 5221 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 5281 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 5341 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg 5401 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 5461 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 5521 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 5581 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 5641 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct 5701 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 5761 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 5821 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg 5881 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 5941 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 6001 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 6061 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 6121 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa 6181 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 6241 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag 6301 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt 6361 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc 6421 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc 6481 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct 6541 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 6601 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 6661 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 6721 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 6781 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 6841 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 6901 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 6961 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 7021 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 7081 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 7141 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc 7201 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtttt 7261 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc 7321 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg 7381 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca 7441 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca 7501 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag 7561 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac 7621 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc 7681 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct 7741 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc 7801 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg 7861 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc 7921 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat 7981 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag 8041 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat 8101 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg 8161 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca 8221 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga 8281 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc 8341 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 8401 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 8461 ccacctgacg tc

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.