pCMV-DDX21-Myc-6×His-Neo plasm

pCMV-DDX21-Myc-6×His-Neo plasmid

PVT91280

pCMV-DDX21-Myc-6×His-Neo plasmid Information

Plasmid name: pCMV-DDX21(human)-Myc-6×His-Neo
Alias: NM_004728.4;GUA; GURDB; RH-II/GU; RH-II/GuA
Gene lenght: 2349bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: T7
Reverse primer:
Copy number: High
Induction:

pCMV-DDX21-Myc-6×His-Neo plasmid Description

pCMV-DDX21-Myc-6×His-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-DDX21-Myc-6×His-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACGG GCCCTCTAGA CTCGAGCGGC CGCCACTGTG CTGGATATCT GCAGAATTCg 961 ccaccatgcc gggaaaactc cgtagtgacg ctggtttgga atcagacacc gcaatgaaaa 1021 aaggggagac actgcgaaag caaaccgagg agaaagagaa aaaagagaag ccaaaatctg 1081 ataagactga agagatagca gaagaggaag aaactgtttt ccccaaagct aaacaagtta 1141 aaaagaaagc agagccttct gaagttgaca tgaattctcc taaatccaaa aaggcaaaaa 1201 agaaagagga gccatctcaa aatgacattt ctcctaaaac caaaagtttg agaaagaaaa 1261 aggagcccat tgaaaagaaa gtggtttctt ctaaaaccaa aaaagtgaca aaaaatgagg 1321 agccttctga ggaagaaata gatgctccta agcccaagaa gatgaagaaa gaaaaggaaa 1381 tgaatggaga aactagagag aaaagcccca aactgaagaa tggatttcct catcctgaac 1441 cggactgtaa ccccagtgaa gctgccagtg aagaaagtaa cagtgagata gagcaggaaa 1501 tacctgtgga acaaaaagaa ggcgctttct ctaattttcc catatctgaa gaaactatta 1561 aacttctcaa aggccgagga gtgaccttcc tatttcctat acaagcaaag acattccatc 1621 atgtttacag cgggaaggac ttaattgcac aggcacggac aggaactggg aagacattct 1681 cctttgccat ccctttgatt gagaaacttc atggggaact gcaagacagg aagagaggcc 1741 gtgcccctca ggtactggtt cttgcaccta caagagagtt ggcaaatcaa gtaagcaaag 1801 acttcagtga catcacaaaa aagctgtcag tggcttgttt ttatggtgga actccctatg 1861 gaggtcaatt tgaacgcatg aggaatggga ttgatatcct ggttggaaca ccaggtcgta 1921 tcaaagacca catacagaat ggcaaactag atctcaccaa acttaagcat gttgtcctgg 1981 atgaagtgga ccagatgttg gatatgggat ttgctgatca agtggaagag attttaagtg 2041 tggcatacaa gaaagattct gaagacaatc cccaaacatt gcttttttct gcaacttgcc 2101 ctcattgggt atttaatgtt gccaagaaat acatgaaatc tacatatgaa caggtggacc 2161 tgattggtaa aaagactcag aaaacggcaa taactgtgga gcatctggct attaagtgcc 2221 actggactca gagggcagca gttattgggg atgtcatccg agtatatagt ggtcatcaag 2281 gacgcactat catcttttgt gaaaccaaga aagaagccca ggagctgtcc cagaattcag 2341 ctataaagca ggatgctcag tccttgcatg gagacattcc acagaagcaa agggaaatca 2401 ccctgaaagg ttttagaaat ggtagttttg gagttttggt ggcaaccaat gttgctgcac 2461 gtgggttaga catccctgag gttgatttgg ttatacaaag ctctccacca aaggatgtag 2521 agtcctacat tcatcgatcc gggcggacag gcagagctgg aaggacgggg gtgtgcatct 2581 gcttttatca gcacaaggaa gaatatcagt tagtacaagt ggagcaaaaa gcgggaatta 2641 agttcaaacg aataggtgtt ccttctgcaa cagaaataat aaaagcttcc agcaaagatg 2701 ccatcaggct tttggattcc gtgcctccca ctgccattag tcacttcaaa caatcagctg 2761 agaagctgat agaggagaag ggagctgtgg aagctctggc agcagcactg gcccatattt 2821 caggtgccac gtccgtagac cagcgctcct tgatcaactc aaatgtgggt tttgtgacca 2881 tgatcttgca gtgctcaatt gaaatgccaa atattagtta tgcttggaaa gaacttaaag 2941 agcagctggg cgaggagatt gattccaaag tgaagggaat ggtttttctc aaaggaaagc 3001 tgggtgtttg ctttgatgta cctaccgcat cagtaacaga aatacaggag aaatggcatg 3061 attcacgacg ctggcagctc tctgtggcca cagagcaacc agaactggaa ggaccacggg 3121 aaggatatgg aggcttcagg ggacagcggg aaggcagtcg aggcttcagg ggacagcggg 3181 acggaaacag aagattcaga ggacagcggg aaggcagtag aggcccgaga ggacagcgat 3241 caggaggtgg caacaaaagt aacagatccc aaaacaaagg ccagaagcgg agtttcagta 3301 aagcatttgg tcaaAAGCTT GGGCCCGAAC AAAAACTCAT CTCAGAAGAG GATCTGAATA 3361 GCGCCGTCGA CCATCATCAT CATCATCATT GAGTTTAAAC GGTCTCCAGC TTAAGTTTAA 3421 ACCGCTGATC AGCCTCGACT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC 3481 CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG 3541 AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG 3601 ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA 3661 TGGCTTCTGA GGCGGAAAGA ACCAGCTGGG GCTCTAGGGG GTATCCCCAC GCGCCCTGTA 3721 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA 3781 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT 3841 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 3901 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 3961 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC 4021 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC 4081 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTAAT 4141 TCTGTGGAAT GTGTGTCAGT TAGGGTGTGG AAAGTCCCCA GGCTCCCCAG CAGGCAGAAG 4201 TATGCAAAGC ATGCATCTCA ATTAGTCAGC AACCAGGTGT GGAAAGTCCC CAGGCTCCCC 4261 AGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCATAG TCCCGCCCCT 4321 AACTCCGCCC ATCCCGCCCC TAACTCCGCC CAGTTCCGCC CATTCTCCGC CCCATGGCTG 4381 ACTAATTTTT TTTATTTATG CAGAGGCCGA GGCCGCCTCT GCCTCTGAGC TATTCCAGAA 4441 GTAGTGAGGA GGCTTTTTTG GAGGCCTAGG CTTTTGCAAA AAGCTCCCGG GAGCTTGTAT 4501 ATCCATTTTC GGATCTGATC AAGAGACAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA 4561 TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC 4621 ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC 4681 GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC 4741 GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC 4801 TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC 4861 TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC 4921 GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG 4981 TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT 5041 CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT 5101 CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG 5161 ATTCATCGAC TGTGGCCGGC TGGGTGTGGC GGACCGCTAT CAGGACATAG CGTTGGCTAC 5221 CCGTGATATT GCTGAAGAGC TTGGCGGCGA ATGGGCTGAC CGCTTCCTCG TGCTTTACGG 5281 TATCGCCGCT CCCGATTCGC AGCGCATCGC CTTCTATCGC CTTCTTGACG AGTTCTTCTG 5341 AGCGGGACTC TGGGGTTCGA AATGACCGAC CAAGCGACGC CCAACCTGCC ATCACGAGAT 5401 TTCGATTCCA CCGCCGCCTT CTATGAAAGG TTGGGCTTCG GAATCGTTTT CCGGGACGCC 5461 GGCTGGATGA TCCTCCAGCG CGGGGATCTC ATGCTGGAGT TCTTCGCCCA CCCCAACTTG 5521 TTTATTGCAG CTTATAATGG TTACAAATAA AGCAATAGCA TCACAAATTT CACAAATAAA 5581 GCATTTTTTT CACTGCATTC TAGTTGTGGT TTGTCCAAAC TCATCAATGT ATCTTATCAT 5641 GTCTGTATAC CGTCGACCTC TAGCTAGAGC TTGGCGTAAT CATGGTCATA GCTGTTTCCT 5701 GTGTGAAATT GTTATCCGCT CACAATTCCA CACAACATAC GAGCCGGAAG CATAAAGTGT 5761 AAAGCCTGGG GTGCCTAATG AGTGAGCTAA CTCACATTAA TTGCGTTGCG CTCACTGCCC 5821 GCTTTCCAGT CGGGAAACCT GTCGTGCCAG CTGCATTAAT GAATCGGCCA ACGCGCGGGG 5881 AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG 5941 GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA 6001 GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC 6061 CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC 6121 AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG 6181 TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC 6241 CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT 6301 CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG 6361 CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC 6421 TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT 6481 GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT 6541 ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC 6601 AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA 6661 AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC 6721 GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC 6781 CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT 6841 GACAGTTACC AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA 6901 TCCATAGTTG CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT 6961 GGCCCCAGTG CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA 7021 ATAAACCAGC CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC 7081 ATCCAGTCTA TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG 7141 CGCAACGTTG TTGCCATTGC TACAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT 7201 TCATTCAGCT CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA 7261 AAAGCGGTTA GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA 7321 TCACTCATGG TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC 7381 TTTTCTGTGA CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG 7441 AGTTGCTCTT GCCCGGCGTC AATACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA 7501 GTGCTCATCA TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG 7561 AGATCCAGTT CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC 7621 ACCAGCGTTT CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG 7681 GCGACACGGA AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 7741 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 7801 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG TC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.