pLV3-CMV-ISL2-3×FLAG-CopGFP-Ne

pLV3-CMV-ISL2-3×FLAG-CopGFP-Neo plasmid

PVT94139

pLV3-CMV-ISL2-3×FLAG-CopGFP-Neo plasmid Information

Plasmid name: pLV3-CMV-ISL2(human)-3×FLAG-CopGFP-Neo
Alias: NM_145805.3
Gene lenght: 1077bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ISL2-3×FLAG-CopGFP-Neo plasmid Description

pLV3-CMV-ISL2-3×FLAG-CopGFP-Neo is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-ISL2-3×FLAG-CopGFP-Neo plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcgcca ccatggtgga tattattttt cattatcctt ttctgggtgc 2341 tatgggtgat cattccaaga agaagcccgg gacggccatg tgcgtgggct gcgggagtca 2401 gatccacgac cagtttatcc tgcgggtgtc gcccgacctc gagtggcacg cggcctgcct 2461 caagtgtgcc gagtgcagcc agtacctgga cgagacgtgc acgtgcttcg tgagagacgg 2521 gaagacctac tgcaagcggg actatgtcag gctgttcggc atcaagtgcg ccaagtgcca 2581 ggtgggcttc agcagcagcg acctggtgat gagggcgcgg gacagcgtgt accacatcga 2641 gtgcttccgc tgctccgtgt gcagccgcca gctgctgcct ggggacgagt tctcgctgcg 2701 ggagcacgag ctgctctgcc gcgccgacca cggcctcctg ctcgagcgcg ccgcggccgg 2761 cagcccgcgc agccccggcc cgcttcccgg cgcccgcggc ctgcatctgc ccgacgctgg 2821 gtcgggccgg cagcccgcgt tgcgcccgca cgtgcacaag cagacggaga agacgacccg 2881 cgtgcggact gtgctgaacg agaagcagct gcacactctg cggacctgct acgccgccaa 2941 cccgcggccc gacgctctca tgaaggagca gctggtggag atgaccggcc tgagcccgcg 3001 ggtcatccgc gtctggttcc agaacaagcg ctgcaaggac aagaagaaat ccattctcat 3061 gaagcagctg cagcagcagc agcacagcga caagacgagc cttcagggac tgactgggac 3121 gcccctggtg gcgggcagtc ccatccgcca tgagaacgcc gtgcagggca gcgcagtgga 3181 ggtgcagacg taccagccgc cgtggaaggc gctcagcgag tttgccctcc agagcgacct 3241 ggaccaaccc gccttccaac agctggtctc cttctccgag tccggctccc taggcaactc 3301 ctccggcagc gacgtgacct ccctgtcctc gcagctcccg gacaccccca acagtatggt 3361 gccgagtccc gtggagacgg gatccGACTA CAAAGACCAT GACGGTGATT ATAAAGATCA 3421 TGACATCGAC TACAAGGATG ACGATGACAA GTAgcggccg cGaaggatct gcgatcgctc 3481 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg 3541 ggtcggcaat tgaacgggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt 3601 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt 3661 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacagctga agcttcgagg 3721 ggctcgcatc tctccttcac gcgcccgccg ccctacctga ggccgccatc cacgccggtt 3781 gagtcgcgtt ctgccgcctc ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt 3841 aagtttaaag ctcaggtcga gaccgggcct ttgtccggcg ctcccttgga gcctacctag 3901 actcagccgg ctctccacgc tttgcctgac cctgcttgct caactctacg tctttgtttc 3961 gttttctgtt ctgcgccgtt acagatccaa gctgtgaccg gcgcctacgc tagacgccac 4021 catggagagc gacgagagcg gcctgcccgc catggagatc gagtgccgca tcaccggcac 4081 cctgaacggc gtggagttcg agctggtggg cggcggagag ggcaccccca agcagggccg 4141 catgaccaac aagatgaaga gcaccaaagg cgccctgacc ttcagcccct acctgctgag 4201 ccacgtgatg ggctacggct tctaccactt cggcacctac cccagcggct acgagaaccc 4261 cttcctgcac gccatcaaca acggcggcta caccaacacc cgcatcgaga agtacgagga 4321 cggcggcgtg ctgcacgtga gcttcagcta ccgctacgag gccggccgcg tgatcggcga 4381 cttcaaggtg gtgggcaccg gcttccccga ggacagcgtg atcttcaccg acaagatcat 4441 ccgcagcaac gccaccgtgg agcacctgca ccccatgggc gataacgtgc tggtgggcag 4501 cttcgcccgc accttcagcc tgcgcgacgg cggctactac agcttcgtgg tggacagcca 4561 catgcacttc aagagcgcca tccaccccag catcctgcag aacgggggcc ccatgttcgc 4621 cttccgccgc gtggaggagc tgcacagcaa caccgagctg ggcatcgtgg agtaccagca 4681 cgccttcaag acccccatcg ccttcgccag atcccgcgct cagtcgtcca attctgccgt 4741 ggacggcacc gccggacccg gctccaccgg atctcgcgag ggcagaggaa gtcttctaac 4801 atgcggtgac gtggaggaga atcccggccc tGCCACCatg gctgaacaag atggattgca 4861 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac 4921 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt 4981 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc 5041 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg 5101 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc 5161 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc 5221 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat 5281 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc 5341 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca 5401 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga 5461 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat 5521 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc 5581 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gaGTCGACAA 5641 TCAACCTCTG GATTACAAAA TTTGTGAAAG ATTGACTGGT ATTCTTAACT ATGTTGCTCC 5701 TTTTACGCTA TGTGGATACG CTGCTTTAAT GCCTTTGTAT CATGCTATTG CTTCCCGTAT 5761 GGCTTTCATT TTCTCCTCCT TGTATAAATC CTGGTTGCTG TCTCTTTATG AGGAGTTGTG 5821 GCCCGTTGTC AGGCAACGTG GCGTGGTGTG CACTGTGTTT GCTGACGCAA CCCCCACTGG 5881 TTGGGGCATT GCCACCACCT GTCAGCTCCT TTCCGGGACT TTCGCTTTCC CCCTCCCTAT 5941 TGCCACGGCG GAACTCATCG CCGCCTGCCT TGCCCGCTGC TGGACAGGGG CTCGGCTGTT 6001 GGGCACTGAC AATTCCGTGG TGTTGTCGGG GAAATCATCG TCCTTTCCTT GGCTGCTCGC 6061 CTGTGTTGCC ACCTGGATTC TGCGCGGGAC GTCCTTCTGC TACGTCCCTT CGGCCCTCAA 6121 TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG 6181 CCTTCGCCCT CAGACGAGTC GGATCTCCCT TTGGGCCGCC TCCCCGCggt acctttaaga 6241 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg 6301 gaagggctaa ttcactccca acgaaGataa gatctgcttt ttgcttgtac tgggtctctc 6361 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag 6421 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct 6481 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt 6541 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag 6601 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 6661 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 6721 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccagttcc gcccattctc 6781 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 6841 agctattcca gaagtagtga ggaggctttt ttggaggcct agacttttgc agagacggcc 6901 caaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 6961 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 7021 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 7081 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 7141 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 7201 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 7261 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 7321 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 7381 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 7441 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 7501 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 7561 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 7621 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 7681 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 7741 actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct 7801 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 7861 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 7921 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 7981 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 8041 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 8101 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 8161 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 8221 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 8281 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 8341 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 8401 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 8461 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 8521 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 8581 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 8641 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 8701 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 8761 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 8821 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 8881 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 8941 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 9001 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 9061 tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta 9121 tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc 9181 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc 9241 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc 9301 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 9361 aataccgcat caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg 9421 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa 9481 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc 9541 tg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.