pLV3-CMV-3×FLAG-NOTCH3-EF1a-Co

pLV3-CMV-3×FLAG-NOTCH3-EF1a-CopGFP-Puro plasmid

PVT94108

pLV3-CMV-3×FLAG-NOTCH3-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-3×FLAG-NOTCH3(human-gc-opt)-EF1a-CopGFP-Puro
Alias: NM_000435.3;CADASIL; CADASIL1; CASIL; IMF2; LMNS;
Gene lenght: 6963bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pLV3-CMV-3×FLAG-NOTCH3-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-3×FLAG-NOTCH3-EF1a-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-3×FLAG-NOTCH3-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 caccatgGAC TACAAGGATG ACGATGACAA GGATTACAAA GACGACGATG ATAAGGACTA 2341 TAAGGATGAT GACGACAAAg aattcATGGG ACCTGGAGCT AGGGGAAGAC GCAGGCGTAG 2401 AAGGCCAATG AGTCCACCTC CTCCGCCTCC TCCAGTGCGA GCACTTCCTC TCCTGCTTCT 2461 TCTCGCAGGA CCTGGAGCCG CTGCTCCCCC CTGTCTCGAT GGGTCCCCCT GTGCCAACGG 2521 GGGCAGGTGC ACCCAGCTTC CTAGCCGAGA AGCTGCGTGT CTGTGTCCAC CTGGATGGGT 2581 Cggtgagcgg tgtcagctgg aggacccctg tcactcaggc ccctgtgctg gccgtggtgt 2641 ctgccagagt tcagtggtgg ctggcaccgc ccgattctca tgccggtgcc cccgtggctt 2701 ccgaggccct gactgctccc tgccagatcc ctgcctcagc agcccttgtg cccacggtgc 2761 ccgctgctca gtggggcccg atggacgctt cctctgctcc tgcccacctg gctaccaggg 2821 ccgcagctgc cgaagcgacg tggatgagtg ccgggtgggt gagccctgcc gccatggtgg 2881 cacctgcctc aacacacctg gctccttccg ctgccagtgt ccagctggct acacagggcc 2941 actatgtgag aaccccgcgg tgccctgtgc accctcacca tgccgtaacg ggggcacctg 3001 caggcagagt ggcgacctca cttacgactg tgcctgtctt cctgggtttg agggtcagaa 3061 ttgtgaagtg aacgtggacg actgtccagg acaccgatgt ctcaatgggg ggacatgcgt 3121 ggatggcgtc aacacctata actgccagtg ccctcctgag tggacaggcc agttctgcac 3181 ggaggacgtg gatgagtgtc agctgcagcc caacgcctgc cacaatgggg gtacctgctt 3241 caacacgctg ggtggccaca gctgcgtgtg tgtcaatggc tggacaggcg agagctgcag 3301 tcagaatatc gatgactgtg ccacagccgt gtgcttccat ggggccacct gccatgaccg 3361 cgtggcttct ttctactgtg cctgccccat gggcaagact ggcctcctgt gtcacctgga 3421 tgacgcctgt gtcagcaacc cctgccacga ggatgctatc tgtgacacaa atccggtgaa 3481 cggccgggcc atttgcacct gtcctcccgg cttcacgggt ggggcatgtg accaggatgt 3541 ggacgagtgc tctatcggcg ccaacccctg cgagcacttg ggcaggtgcg tgaacacgca 3601 gggctccttc ctgtgccagt gcggtcgtgg ctacactgga cctcgctgtg agaccgatgt 3661 caacgagtgt ctgtcggggc cctgccgaaa ccaggccacg tgcctcgacc gcataggcca 3721 gttcacctgt atctgtatgg caggcttcac aggaacctat tgcgaggtgg acattgacga 3781 gtgtcagagt agcccctgtg tcaacggtgg ggtctgcaag gaccgagtca atggcttcag 3841 ctgcacctgc ccctcgggct tcagcggctc cacgtgtcag ctggacgtgg acgaatgcgc 3901 cagcacgccc tgcaggaatg gcgccaaatg cgtggaccag cccgatggct acgagtgccg 3961 ctgtgccgag ggctttgagg gcacgctgtg tgatcgcaac gtggacgact gctcccctga 4021 cccatgccac catggtcgct gcgtggatgg catcgccagc ttctcatgtg cctgtgctcc 4081 tggctacacg ggcacacgct gcgagagcca ggtggacgaa tgccgcagcc agccctgccg 4141 ccatggcggc aaatgcctag acctggtgga caagtacctc tgccgctgcc cttctgggac 4201 cacaggtgtg aactgcgaag tgaacattga cgactgtgcc agcaacccct gcacctttgg 4261 agtctgccgt gatggcatca accgctacga ctgtgtctgc caacctggct tcacagggcc 4321 cctttgtaac gtggagatca atgagtgtgc ttccagccca tgcggcgagg gaggttcctg 4381 tgtggatggg gaaaatggct tccgctgcct ctgcccgcct ggctccttgc ccccactctg 4441 cctccccccg agccatccct gtgcccatga gccctgcagt cacggcatct gctatgatgc 4501 acctggcggg ttccgctgtg tgtgtgagcc tggctggagt ggcccccgct gcagccagag 4561 cctggcccga gacgcctgtg agtcccagcc gtgcagggcc ggtgggacat gcagcagcga 4621 tggaatgggt ttccactgca cctgcccgcc tggtgtccag ggacgtcagt gtgaactcct 4681 ctccccctgc accccgaacc cctgtgagca tgggggccgc tgcgagtctg cccctggcca 4741 gctgcctgtc tgctcctgcc cccagggctg gcaaggccca cgatgccagc aggatgtgga 4801 cgagtgtgct ggccccgcac cctgtggccc tcatggtatc tgcaccaacc tggcagggag 4861 tttcagctgc acctgccatg gagggtacac tggcccttcc tgcgatcagg acatcaatga 4921 ctgtgacccc aacccatgcc tgaacggtgg ctcgtgccaa gacggcgtgg gctccttttc 4981 ctgctcctgc ctccctggtt tcgccggccc acgatgcgcc cgcgatgtgg atgagtgcct 5041 gagcaacccc tgcggcccgg gcacctgtac cgaccacgtg gcctccttca cctgcacctg 5101 cccgccaggc tacggaggct tccactgcga acaggacctg cccgactgca gccccagctc 5161 ctgcttcaat ggcgggacct gtgtggacgg cgtgaactcg ttcagctgcc tgtgccgtcc 5221 cggctacaca ggagcccact gccaacatga ggcagacccc tgcctctcgc ggccctgcct 5281 acacgggggc gtctgcagcg ccgcccaccc tggcttccgc tgcacctgcc tcgagagctt 5341 cacgggcccg cagtgccaga cgctggtgga ttggtgcagc cgccagcctt gtcaaaacgg 5401 gggtcgctgc gtccagactg gggcctattg cctttgtccc cctggatgga gcggacgcct 5461 ctgtgacatc cgaagcttgc cctgcaggga ggccgcagcc cagatcgggg tgcggctgga 5521 gcagctgtgt caggcgggtg ggcagtgtgt ggatgaagac agctcccact actgcgtgtg 5581 cccagagggc cgtactggta gccactgtga gcaggaggtg gacccctgct tggcccagcc 5641 ctgccagcat ggggggacct gccgtggcta tatggggggc tacatgtgtg agtgtcttcc 5701 tggctacaat ggtgataact gtgaggacga cgtggacgag tgtgcctccc agccctgcca 5761 gcacgggggt tcatgcattg acctcgtggc ccgctatctc tgctcctgtc ccccaggaac 5821 gctgggggtg ctctgcgaga ttaatgagga tgactgcggc ccaggcccac cgctggactc 5881 agggccccgg tgcctacaca atggcacctg cgtggacctg gtgggtggtt tccgctgcac 5941 ctgtccccca ggatacactg gtttgcgctg cgaggcagac atcaatgagt gtcgctcagg 6001 tgcctgccac gcggcacaca cccgggactg cctgcaggac ccaggcggag gtttccgttg 6061 cctttgtcat gctggcttct caggtcctcg ctgtcagact gtcctgtctc cctgcgagtc 6121 ccagccatgc cagcatggag gccagtgccg tcctagcccg ggtcctgggg gtgggctgac 6181 cttcacctgt cactgtgccc agccgttctg gggtccgcgt tgcgagcggg tggcgcgctc 6241 ctgccgggag ctgcagtgcc cggtgggcgt cccatgccag cagacgcccc gcgggccgcg 6301 ctgcgcctgc cccccagggt tgtcgggacc ctcctgccgc agcttcccgg ggtcgccgcc 6361 gggggccagc aacgccagct gcgcggccgc cccctgtctc cacgggggct cctgccgccc 6421 cgcgccgctc gcgcccttct tccgctgcgc ttgcgcgcag ggctggaccg ggccgcgctg 6481 cgaggcgccc gccgcggcac ccgaggtctc ggaggagccg cggtgcccgc gcgccgcctg 6541 ccaggccaag cgcggggacc agcgctgcga ccgcgagtgc aacagcccag gctgcggctg 6601 ggacggcggc gactgctcgc tgagcgtggg cgacccctgg cggcaatgcg aggcgctgca 6661 gtgctggcgc ctcttcaaca acagccgctg cgaccccgcc tgcagctcgc ccgcctgcct 6721 ctacgacaac ttcgactgcc acgccggtgg ccgcgagcgc acttgcaacc cggtgtacga 6781 gaagtactgc gccgaccact ttgccgacgg ccgctgcgac cagggctgca acacggagga 6841 gtgcggctgg gatgggctgg attgtgccag cgaggtgccg gccctgctgg cccgcggcgt 6901 gctggtgctc acagtgctgc tgccgccaga ggagctactg cgttccagcg ccgactttct 6961 gcagcggctc agcgccatcc tgcgcacctc gctgcgcttc cgcctggacg cgcacggcca 7021 ggccatggtc ttcccttacc accggcctag tcctggctcc gaaccccggg cccgtcggga 7081 gctggccccc gaggtgatcg gctcggtagt aatgctggag attgacaacc ggctctgcct 7141 gcagtcgcct gagaatgatc actgcttccc cgatgcccag agcgccgctg actacctggg 7201 agcgttgtca gcggtggagc gcctggactt cccgtaccca ctgcgggacg tgcgggggga 7261 gccgctggag cctccagaac ccagcgtccc gctgctgcca ctgctagtgg cgggcgctgt 7321 cttgctgctg gtcattctcg tcctgggtgt catggtggcc cggcgcaagc gcgagcacag 7381 caccctctgg ttccctgagg gcttctcact gcacaaggac gtggcctctg gtcacaaggg 7441 ccggcgggaa cccgtgggcc aggacgcgct gggcatgaag aacatggcca agggtgagag 7501 cctgatgggg gaggtggcca cagactggat ggacacagag tgcccagagg ccaagcggct 7561 aaaggtagag gagccaggca tgggggctga ggaggctgtg gattgccgtc agtggactca 7621 acaccatctg gttgctgctg acatccgcgt ggcaccagcc atggcactga caccaccaca 7681 gggcgacgca gatgctgatg gcatggatgt caatgtgcgt ggcccagatg gcttcacccc 7741 gctaatgctg gcttccttct gtgggggggc tctggagcca atgccaactg aagaggatga 7801 ggcagatgac acatcagcta gcatcatctc cgacctgatc tgccaggggg ctcagcttgg 7861 ggcacggact gaccgtactg gcgagactgc tttgcacctg gctgcccgtt atgcccgtgc 7921 tgatgcagcc aagcggctgc tggatgctgg ggcagacacc aatgcccagg accactcagg 7981 ccgcactccc ctgcacacag ctgtcacagc cgatgcccag ggtgtcttcc agattctcat 8041 ccgaaaccgc tctacagact tggatgcccg catggcagat ggctcaacgg cactgatcct 8101 ggcggcccgc ctggcagtag agggcatggt ggaagagctc atcgccagcc atgctgatgt 8161 caatgctgtg gatgagcttg ggaaatcagc cttacactgg gctgcggctg tgaacaacgt 8221 ggaagccact ttggccctgc tcaaaaatgg agccaataag gacatgcagg atagcaagga 8281 ggagaccccc ctattcctgg ccgcccgcga gggcagctat gaggctgcca agctgctgtt 8341 ggaccacttt gccaaccgtg agatcaccga ccacctggac aggctgccgc gggacgtagc 8401 ccaggagaga ctgcaccagg acatcgtgcg cttgctggat caacccagtg ggccccgcag 8461 cccccccggt ccccacggcc tggggcctct gctctgtcct ccaggggcct tcctccctgg 8521 cctcaaagcg gcacagtcgg ggtccaagaa gagcaggagg ccccccggga aggcggggct 8581 ggggccgcag gggccccggg ggcggggcaa gaagctgacg ctggcctgcc cgggccccct 8641 ggctgacagc tcggtcacgc tgtcgcccgt ggactcgctg gactccccgc ggcctttcgg 8701 tgggccccct gcttcccctg gtggcttccc ccttgagggg ccctatgcag ctgccactgc 8761 cactgcagtg tctctggcac agcttggtgg cccaggccgg gcgggtctag ggcgccagcc 8821 ccctggagga tgtgtactca gcctgggcct gctgaaccct gtggctgtgc ccctcgattg 8881 ggcccggctg cccccacctg cccctccagg cccctcgttc ctgctgccac tggcgccggg 8941 accccagctg ctcaacccag ggacccccgt ctccccgcag gagcggcccc cgccttacct 9001 ggcagtccca ggacatggcg aggagtaccc ggcggctggg gcacacagca gccccccaaa 9061 ggcccgcttc ctgcgggttc ccagtgagca cccttacctg accccatccc ccgaatcccc 9121 tgagcactgg gccagcccct cacctccctc cctctcagac tggtccgaat ccacgcctag 9181 cccagccact gccactgggg ccatggccac caccactggg gcactgcctg cccagccact 9241 tcccttgtct gttcccagct cccttgctca ggcccagacc cagctggggc cccagccgga 9301 agttaccccc aagaggcaag tgttggcctg aggatccgcg gccgcgaagg atctgcgatc 9361 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg 9421 gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg 9481 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag 9541 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc 9601 gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc 9661 ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct 9721 aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac 9781 ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg 9841 tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgctagacg 9901 ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg 9961 gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc cccaagcagg 10021 gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc ccctacctgc 10081 tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc ggctacgaga 10141 accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc gagaagtacg 10201 aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg 10261 gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc accgacaaga 10321 tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac gtgctggtgg 10381 gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc gtggtggaca 10441 gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg ggccccatgt 10501 tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc gtggagtacc 10561 agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg tccaattctg 10621 ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga ggaagtcttc 10681 taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag cccacggtgc 10741 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg 10801 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc 10861 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg 10921 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg 10981 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga 11041 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg 11101 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg 11161 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc 11221 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc 11281 gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat tacaaaattt 11341 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 11401 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 11461 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 11521 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 11581 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 11641 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 11701 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc 11761 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 11821 gcctgctgcc ggctctgcgg cctcttccgc gtctccgcct tcgccctcag acgagtcgga 11881 tctccctttg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg 11941 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 12001 gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct 12061 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 12121 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 12181 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 12241 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt 12301 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 12361 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc 12421 tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 12481 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 12541 aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata 12601 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 12661 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 12721 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 12781 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 12841 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 12901 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 12961 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 13021 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 13081 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 13141 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 13201 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 13261 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 13321 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 13381 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 13441 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 13501 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 13561 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 13621 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 13681 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 13741 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 13801 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 13861 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 13921 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 13981 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 14041 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 14101 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 14161 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 14221 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 14281 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 14341 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 14401 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 14461 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 14521 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 14581 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 14641 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 14701 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 14761 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 14821 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 14881 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 14941 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 15001 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 15061 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 15121 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 15181 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.