pLV2-HSP90B1-3×HA-Puro plasmid

pLV2-HSP90B1-3×HA-Puro plasmid

PVT92989

pLV2-HSP90B1-3×HA-Puro plasmid Information

Plasmid name: pLV2-HSP90B1(human)-3×HA-Puro
Alias: NM_003299.3;ECGP; GP96; GRP94; HEL-S-125m; HEL35; TRA1
Gene lenght: 2409bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-HSP90B1-3×HA-Puro plasmid Description

pLV2-HSP90B1-3×HA-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-HSP90B1-3×HA-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 ctcaagcttc gaattcGCCA CCATGAGGGC CCTGTGGGTG CTGGGCCTCT GCTGCGTCCT 2881 GCTGACCTTC GGGTCGGTCA GAGCTGACGA TGAAGTTGAT GTGGATGGTA CAGTAGAAGA 2941 GGATCTGGGT AAAAGTAGAG AAGGATCAAG GACGGATGAT GAAGTAGTAC AGAGAGAGGA 3001 AGAAGCTATT CAGTTGGATG GATTAAATGC ATCACAAATA AGAGAACTTA GAGAGAAGTC 3061 GGAAAAGTTT GCCTTCCAAG CCGAAGTTAA CAGAATGATG AAACTTATCA TCAATTCATT 3121 GTATAAAAAT AAAGAGATTT TCCTGAGAGA ACTGATTTCA AATGCTTCTG ATGCTTTAGA 3181 TAAGATAAGG CTAATATCAC TGACTGATGA AAATGCTCTT TCTGGAAATG AGGAACTAAC 3241 AGTCAAAATT AAGTGTGATA AGGAGAAGAA CCTGCTGCAT GTCACAGACA CCGGTGTAGG 3301 AATGACCAGA GAAGAGTTGG TTAAAAACCT TGGTACCATA GCCAAATCTG GGACAAGCGA 3361 GTTTTTAAAC AAAATGACTG AAGCACAGGA AGATGGCCAG TCAACTTCTG AATTGATTGG 3421 CCAGTTTGGT GTCGGTTTCT ATTCCGCCTT CCTTGTAGCA GATAAGGTTA TTGTCACTTC 3481 AAAACACAAC AACGATACCC AGCACATCTG GGAGTCTGAC TCCAATGAAT TTTCTGTAAT 3541 TGCTGACCCA AGAGGAAACA CTCTAGGACG GGGAACGACA ATTACCCTTG TCTTAAAAGA 3601 AGAAGCATCT GATTACCTTG AATTGGATAC AATTAAAAAT CTCGTCAAAA AATATTCACA 3661 GTTCATAAAC TTTCCTATTT ATGTATGGAG CAGCAAGACT GAAACTGTTG AGGAGCCCAT 3721 GGAGGAAGAA GAAGCAGCCA AAGAAGAGAA AGAAGAATCT GATGATGAAG CTGCAGTAGA 3781 GGAAGAAGAA GAAGAAAAGA AACCAAAGAC TAAAAAAGTT GAAAAAACTG TCTGGGACTG 3841 GGAACTTATG AATGATATCA AACCAATATG GCAGAGACCA TCAAAAGAAG TAGAAGAAGA 3901 TGAATACAAA GCTTTCTACA AATCATTTTC AAAGGAAAGT GATGACCCCA TGGCTTATAT 3961 TCACTTTACT GCTGAAGGGG AAGTTACCTT CAAATCAATT TTATTTGTAC CCACATCTGC 4021 TCCACGTGGT CTGTTTGACG AATATGGATC TAAAAAGAGC GATTACATTA AGCTCTATGT 4081 GCGCCGTGTA TTCATCACAG ACGACTTCCA TGATATGATG CCTAAATACC TCAATTTTGT 4141 CAAGGGTGTG GTGGACTCAG ATGATCTCCC CTTGAATGTT TCCCGCGAGA CTCTTCAGCA 4201 ACATAAACTG CTTAAGGTGA TTAGGAAGAA GCTTGTTCGT AAAACGCTGG ACATGATCAA 4261 GAAGATTGCT GATGATAAAT ACAATGATAC TTTTTGGAAA GAATTTGGTA CCAACATCAA 4321 GCTTGGTGTG ATTGAAGACC ACTCGAATCG AACACGTCTT GCTAAACTTC TTAGGTTCCA 4381 GTCTTCTCAT CATCCAACTG ACATTACTAG CCTAGACCAG TATGTGGAAA GAATGAAGGA 4441 AAAACAAGAC AAAATCTACT TCATGGCTGG GTCCAGCAGA AAAGAGGCTG AATCTTCTCC 4501 ATTTGTTGAG CGACTTCTGA AAAAGGGCTA TGAAGTTATT TACCTCACAG AACCTGTGGA 4561 TGAATACTGT ATTCAGGCCC TTCCCGAATT TGATGGGAAG AGGTTCCAGA ATGTTGCCAA 4621 GGAAGGAGTG AAGTTCGATG AAAGTGAGAA AACTAAGGAG AGTCGTGAAG CAGTTGAGAA 4681 AGAATTTGAG CCTCTGCTGA ATTGGATGAA AGATAAAGCC CTTAAGGACA AGATTGAAAA 4741 GGCTGTGGTG TCTCAGCGCC TGACAGAATC TCCGTGTGCT TTGGTGGCCA GCCAGTACGG 4801 ATGGTCTGGC AACATGGAGA GAATCATGAA AGCACAAGCG TACCAAACGG GCAAGGACAT 4861 CTCTACAAAT TACTATGCGA GTCAGAAGAA AACATTTGAA ATTAATCCCA GACACCCGCT 4921 GATCAGAGAC ATGCTTCGAC GAATTAAGGA AGATGAAGAT GATAAAACAG TTTTGGATCT 4981 TGCTGTGGTT TTGTTTGAAA CAGCAACGCT TCGGTCAGGG TATCTTTTAC CAGACACTAA 5041 AGCATATGGA GATAGAATAG AAAGAATGCT TCGCCTCAGT TTGAACATTG ACCCTGATGC 5101 AAAGGTGGAA GAAGAGCCCG AAGAAGAACC TGAAGAGACA GCAGAAGACA CAACAGAAGA 5161 CACAGAGCAA GACGAAGATG AAGAAATGGA TGTGGGAACA GATGAAGAAG AAGAAACAGC 5221 AAAGGAATCT ACAGCTGAAA AAGATGAATT GggatccTAC CCATACGACG TACCTGATTA 5281 TGCAACTTAT CCTTATGATG TACCTGATTA TGCTAGCTAT CCTTATGATG TCCCAGACTA 5341 CGCATAGtct agataattct accgggtagg ggaggcgctt ttcccaaggc agtctggagc 5401 atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct ggcctcgcac 5461 acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc ccttcgcgcc 5521 accttctact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg cgtcgtgcag 5581 gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca gcaccgctga 5641 gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct ccttcgcttt 5701 ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg ggctcagggg 5761 cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc aaaagcgcac 5821 gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca gcccaagctt 5881 accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc 5941 gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac cgtcgatccg 6001 gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg cgtcgggctc 6061 gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg gaccacgccg 6121 gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc 6181 ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca ccggcccaag 6241 gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg caagggtctg 6301 ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt gcccgccttc 6361 ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt caccgtcacc 6421 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc 6481 tgaccgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat 6541 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca 6601 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc 6661 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc 6721 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt 6781 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg 6841 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc 6901 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta 6961 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg 7021 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc 7081 cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc 7141 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg 7201 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat 7261 cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga 7321 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac 7381 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta 7441 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac 7501 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac 7561 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc 7621 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg 7681 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg 7741 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 7801 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 7861 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 7921 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 7981 atcagagagt gagaggcctt gacattgcta gcgttttacc gtcgacctct agctagagct 8041 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 8101 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 8161 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 8221 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 8281 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 8341 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 8401 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 8461 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 8521 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 8581 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 8641 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 8701 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 8761 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 8821 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 8881 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 8941 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 9001 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 9061 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 9121 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 9181 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 9241 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 9301 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 9361 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 9421 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 9481 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 9541 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 9601 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 9661 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 9721 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 9781 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 9841 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 9901 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 9961 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 10021 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 10081 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 10141 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 10201 cacctgacgt cgacggatcg ggagatcaac ttgtttattg cagcttataa tggttacaaa 10261 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 10321 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct 10381 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg 10441 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt 10501 tgttaaatat gtactacaaa cttagtagtt tttaaagaaa ttgtatttgt taaatatgta 10561 ctacaaactt agtagt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.