pLV3-CMV-3×FLAG-MIGA2-EF1a-Cop

pLV3-CMV-3×FLAG-MIGA2-EF1a-CopGFP-Puro plasmid

PVT91972

pLV3-CMV-3×FLAG-MIGA2-EF1a-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-3×FLAG-MIGA2(human)-EF1a-CopGFP-Puro
Alias: NM_032809.4;C9orf54; FAM73B
Gene lenght: 1971bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-3×FLAG-MIGA2-EF1a-CopGFP-Puro plasmid Description

pLV3-CMV-3×FLAG-MIGA2-EF1a-CopGFP-Puro It is a plasmid suitable for protein expression in mammalian cells and can also be used for lentivirus packaging.

pLV3-CMV-3×FLAG-MIGA2-EF1a-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 caccatgGAC TACAAGGATG ACGATGACAA GGATTACAAA GACGACGATG ATAAGGACTA 2341 TAAGGATGAT GACGACAAAg aattcatgga aggagcaggc gtgcgggccg tgagcggcgc 2401 cagagggtac ctggctctgt ggaggggccc tctggtatgt gtgtccctgt ccttctgggg 2461 cgtggatggt gcctgggacc cagctggcaa ccagttgaag acgttctcct tggaagctct 2521 tggccctgag gactttgcct ggggcattgg ccctgccatg gcgttccgga gggccgaggg 2581 cacgtctatg atccaggccc tggccatgac ggtggccgag atccccgtgt tcctgtacac 2641 gacgtttggg cagtctgcat tctcccagct acggttgacg ccaggcctgc ggaaagtcct 2701 ctttgccacg gccctgggga ctgtggccct ggccctggct gcccaccagc tgaagaggcg 2761 acggaggagg aagaagcagg ttggtcccga gatgggaggg gagcagctgg gcacggtgcc 2821 cctccctatc ctcttggcca ggaaggtccc ttcagtgaag aaaggatact ccagccggag 2881 agtccagagc cccagcagca agagcaacga caccctgagt ggcatctctt ccattgagcc 2941 cagcaagcac tcgggctcct cccacagtgt ggcctcgatg atggcagtga actcatccag 3001 ccccacagcc gcgtgctcgg gactatggga tgccagaggg atggaggagt ctctgaccac 3061 cagcgacggc aatgcagaga gcctgtacat gcaaggcatg gagctgtttg aggaagctct 3121 gcagaagtgg gagcaggcac taagcgtggg ccagcggggg gacagcggca gcacccccat 3181 gcccagggac ggcctccgga acccagagac tgcatcagag ccactgtctg agccagagtc 3241 acagcggaag gagtttgcag agaagctgga gtccctgctg caccgtgcct accacctgca 3301 ggaggagttc ggctccacct tccccgcaga cagcatgctg ctagacctcg agaggaccct 3361 catgctgccc ctgaccgagg gctcgctgcg gctgcgggcg gacgatgagg acagcctgac 3421 ttcagaggat tccttcttct ccgccaccga gctctttgag tccctgcaga ctggagatta 3481 cccgatccca ctctccagac ccgccgctgc ctatgaggag gccctgcagc tggtgaagga 3541 ggggagagtg ccttgccgga ccctcaggac ggagctgctg ggctgctaca gtgaccagga 3601 ctttctggcc aagctgcact gtgtgcggca ggccttcgag gggcttctgg aagacaagag 3661 taaccagctt ttcttcggga aagtgggccg acagatggtg acaggcctga tgaccaaggc 3721 tgagaagagc cccaaaggct tcctggagag ctacgaggag atgctgagct atgccctgcg 3781 gcccgagacc tgggccacaa cacggctgga gctggagggc cgaggggtgg tatgcatgag 3841 cttcttcgac atcgtgctgg acttcatcct catggacgcc ttcgaggacc tggagaaccc 3901 tccggcctcg gtgctcgccg tcctgcggaa ccgctggctg tcagacagct tcaaggagac 3961 ggccttggcc actgcttgct ggtcggtcct gaaagccaag aggaggctgc tgatggtgcc 4021 tgatggcttc atctcccatt tctactccgt atcggagcat gtgagccctg tcctagcctt 4081 cggcttcctt ggacccaagc ctcagcttgc tgaagtctgt gctttcttca agcaccagat 4141 tgtgcagtac ctgagggaca tgttcgacct ggacaatgtg cgctacacgt cactgcccgc 4201 gctggcagac gacatcctgc agctgtcccg gcgccgcagc gagatattgc tggggtacct 4261 gggggtgccc gcggccagca gcgcaggcgt gaatggggcg ctgccccgag agaatgggcc 4321 cctgggggag ctgcagTAGg gatccgcggc cgcgaaggat ctgcgatcgc tccggtgccc 4381 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca 4441 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact 4501 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga 4561 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca 4621 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg 4681 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa 4741 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc 4801 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg 4861 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggaga 4921 gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg 4981 gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca 5041 acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga 5101 tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc 5161 acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg 5221 tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg 5281 tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca 5341 acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc 5401 gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact 5461 tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc 5521 gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca 5581 agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca 5641 ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta acatgcggtg 5701 acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc 5761 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca 5821 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct 5881 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg 5941 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc 6001 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc 6061 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg 6121 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc 6181 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc 6241 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca 6301 tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt gaaagattga 6361 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 6421 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 6481 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 6541 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 6601 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 6661 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc 6721 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 6781 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 6841 ctctgcggcc tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggc 6901 cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 6961 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc 7021 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 7081 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 7141 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 7201 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 7261 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca 7321 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 7381 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct 7441 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 7501 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 7561 aggcctagac ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt 7621 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 7681 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 7741 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 7801 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 7861 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 7921 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 7981 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 8041 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 8101 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 8161 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 8221 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 8281 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 8341 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 8401 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 8461 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 8521 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 8581 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 8641 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 8701 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 8761 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 8821 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 8881 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 8941 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 9001 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 9061 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 9121 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 9181 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 9241 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 9301 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 9361 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 9421 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 9481 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 9541 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 9601 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 9661 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 9721 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 9781 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 9841 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 9901 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 9961 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 10021 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 10081 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 10141 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 10201 ttgtaaaacg acggccagtg ccaagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.