pLV3-EF1a-Il5ra-3×FLAG-PGK-Cop

pLV3-EF1a-Il5ra-3×FLAG-PGK-CopGFP-Puro plasmid

PVT94028

pLV3-EF1a-Il5ra-3×FLAG-PGK-CopGFP-Puro plasmid Information

Plasmid name: pLV3-EF1a-Il5ra(mouse)-3×FLAG-PGK-CopGFP-Puro
Alias: NM_008370.2;CD125/CDw125/Il5r
Gene lenght: 1245bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: EF1a
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: M13F-72
Reverse primer:
Copy number: High
Induction:

pLV3-EF1a-Il5ra-3×FLAG-PGK-CopGFP-Puro plasmid Description

pLV3-EF1a-Il5ra-3×FLAG-PGK-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-EF1a-Il5ra-3×FLAG-PGK-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtaag gatctgcgat cgctccggtg cccgtcagtg ggcagagcgc acatcgccca 1981 cagtccccga gaagttgggg ggaggggtcg gcaattgaac gggtgcctag agaaggtggc 2041 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg 2101 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg 2161 ccagaacaca gctgaagctt cgaggggctc gcatctctcc ttcacgcgcc cgccgcccta 2221 cctgaggccg ccatccacgc cggttgagtc gcgttctgcc gcctcccgcc tgtggtgcct 2281 cctgaactgc gtccgccgtc taggtaagtt taaagctcag gtcgagaccg ggcctttgtc 2341 cggcgctccc ttggagccta cctagactca gccggctctc cacgctttgc ctgaccctgc 2401 ttgctcaact ctacgtcttt gtttcgtttt ctgttctgcg ccgttacaga tccaagctgt 2461 gaccggcgcc tactctagag ctagcgaatt cgccaccATG GTGCCTGTGT TACTAATTCT 2521 TGTGGGAGCT TTGGCAACAC TGCAAGCTGA CTTACTTAAT CACAAAAAGT TTTTACTTCT 2581 ACCACCTGTC AATTTTACCA TTAAAGCCAC TGGATTAGCT CAAGTTCTTT TACACTGGGA 2641 CCCAAATCCT GACCAAGAGC AAAGGCATGT TGATCTAGAG TATCACGTGA AAATAAATGC 2701 CCCACAAGAA GACGAATATG ATACCAGAAA GACTGAAAGC AAATGTGTGA CCCCCCTTCA 2761 TGAAGGCTTT GCAGCTAGCG TGAGGACCAT TCTGAAGAGC AGCCATACAA CTCTGGCCAG 2821 CAGTTGGGTT TCTGCTGAAC TCAAAGCTCC ACCAGGATCT CCTGGAACCT CGGTTACGAA 2881 TTTAACTTGT ACCACACACA CTGTTGTAAG TAGCCACACC CACTTAAGGC CATACCAAGT 2941 GTCCCTTCGT TGCACCTGGC TTGTTGGGAA GGATGCCCCT GAGGACACAC AGTATTTCCT 3001 ATACTACAGG TTTGGTGTTT TGACTGAAAA ATGCCAAGAA TACAGCAGAG ATGCACTGAA 3061 CAGAAATACT GCATGCTGGT TTCCCAGGAC ATTTATCAAC AGCAAAGGGT TTGAGCAGCT 3121 TGCTGTGCAC ATTAATGGCT CAAGCAAGCG TGCTGCAATC AAGCCCTTTG ATCAGCTGTT 3181 CAGTCCACTT GCCATTGACC AAGTGAATCC TCCAAGGAAT GTCACAGTGG AAATTGAAAG 3241 CAATTCTCTC TATATACAGT GGGAGAAACC ACTTTCTGCC TTTCCAGATC ATTGCTTTAA 3301 CTATGAGCTG AAAATTTACA ACACAAAAAA TGGTCACATT CAGAAGGAAA AACTGATCGC 3361 CAATAAGTTC ATCTCAAAAA TTGATGATGT TTCTACATAT TCCATTCAAG TGAGAGCAGC 3421 TGTGAGCTCA CCTTGCAGAA TGCCAGGAAG GTGGGGCGAG TGGAGTCAAC CTATTTATGT 3481 GGGAAAGGAA AGGAAGTCCT TGGTAGAATG GCATCTCATT GTGCTCCCAA CAGCCGCCTG 3541 CTTCGTCTTG TTAATCTTCT CACTCATCTG CAGAGTGTGT CATCTATGGA CCAGGTTGTT 3601 TCCACCGGTT CCGGCCCCAA AGAGTAACAT CAAAGATCTC CCTGTGGTTA CTGAATATGA 3661 GAAACCTTCG AATGAAACCA AAATTGAAGT TGTACATTGT GTGGAAGAGG TTGGATTTGA 3721 AGTCATGGGA AATTCCACGT TTggatccGA CTACAAAGAC CATGACGGTG ATTATAAAGA 3781 TCATGACATC GACTACAAGG ATGACGATGA CAAGtagcgg ccgctgggta ggggaggcgc 3841 ttttcccaag gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca 3901 caagtggcct ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt 3961 tctttggtgg ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg 4021 ccccgcagct cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg 4081 tgcagatgga cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat 4141 agcagctttg ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg 4201 ggctcagggg cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat 4261 tctgcacgct tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc 4321 tttcgacctg cagcccaagc ttaccatgga gagcgacgag agcggcctgc ccgccatgga 4381 gatcgagtgc cgcatcaccg gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg 4441 agagggcacc cccaagcagg gccgcatgac caacaagatg aagagcacca aaggcgccct 4501 gaccttcagc ccctacctgc tgagccacgt gatgggctac ggcttctacc acttcggcac 4561 ctaccccagc ggctacgaga accccttcct gcacgccatc aacaacggcg gctacaccaa 4621 cacccgcatc gagaagtacg aggacggcgg cgtgctgcac gtgagcttca gctaccgcta 4681 cgaggccggc cgcgtgatcg gcgacttcaa ggtggtgggc accggcttcc ccgaggacag 4741 cgtgatcttc accgacaaga tcatccgcag caacgccacc gtggagcacc tgcaccccat 4801 gggcgataac gtgctggtgg gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta 4861 ctacagcttc gtggtggaca gccacatgca cttcaagagc gccatccacc ccagcatcct 4921 gcagaacggg ggccccatgt tcgccttccg ccgcgtggag gagctgcaca gcaacaccga 4981 gctgggcatc gtggagtacc agcacgcctt caagaccccc atcgccttcg ccagatcccg 5041 cgctcagtcg tccaattctg ccgtggacgg caccgccgga cccggctcca ccggatctcg 5101 cgagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctatgac 5161 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac 5221 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca 5281 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg 5341 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt 5401 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg 5461 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc 5521 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc 5581 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac 5641 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt 5701 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgagtcga 5761 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc 5821 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg 5881 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt 5941 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac 6001 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc 6061 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct 6121 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct 6181 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct 6241 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct 6301 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctggtacctt 6361 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg 6421 gactggaagg gctaattcac tcccaacgaa aataagatct gctttttgct tgtactgggt 6481 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc 6541 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg 6601 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta 6661 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga 6721 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 6781 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 6841 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca gttccgccca 6901 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 6961 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga 7021 cggcccaaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 7081 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 7141 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 7201 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 7261 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 7321 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 7381 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 7441 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 7501 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 7561 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 7621 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 7681 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 7741 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 7801 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 7861 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt 7921 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 7981 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 8041 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 8101 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 8161 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 8221 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 8281 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 8341 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 8401 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 8461 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 8521 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 8581 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 8641 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 8701 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 8761 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 8821 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 8881 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 8941 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 9001 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 9061 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 9121 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 9181 aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag 9241 gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca 9301 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 9361 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact atgcggcatc 9421 agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag 9481 gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt gggaagggcg 9541 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 9601 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtgc 9661 caagctg

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.