pCMV-PTPRF-mNeonGreen-Neo plas

pCMV-PTPRF-mNeonGreen-Neo plasmid

PVT93644

pCMV-PTPRF-mNeonGreen-Neo plasmid Information

Plasmid name: pCMV-PTPRF(human)-mNeonGreen-Neo
Alias: NM_002840.5;BNAH2; LAR
Gene lenght: 5721bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Kan
Screening marker: Neo/G418
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-PTPRF-mNeonGreen-Neo plasmid Description

pCMV-PTPRF-mNeonGreen-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-PTPRF-mNeonGreen-Neo plasmid Sequence

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagccacc 601 atggcccctg agccagcccc agggaggacg atggtgcccc ttgtgcctgc actggtgatg 661 cttggtttgg tggcaggcgc ccatggtgac agcaaacctg tcttcattaa agtccctgag 721 gaccagactg ggctgtcagg aggggtagcc tccttcgtgt gccaagctac aggagaaccc 781 aagccgcgca tcacatggat gaagaagggg aagaaagtca gctcccagcg cttcgaggtc 841 attgagtttg atgatggggc agggtcagtg cttcggatcc agccattgcg ggtgcagcga 901 gatgaagcca tctatgagtg tacagctact aacagcctgg gtgagatcaa cactagtgcc 961 aagctctcag tgctcgaaga ggaacagctg ccccctgggt tcccttccat cgacatgggg 1021 cctcagctga aggtggtgga gaaggcacgc acagccacca tgctatgtgc cgcaggcgga 1081 aatccagacc ctgagatttc ttggttcaag gacttccttc ctgtagaccc tgccacgagc 1141 aacggccgca tcaagcagct gcgttcaggt gccttgcaga tagagagcag tgaggaatcc 1201 gaccaaggca agtacgagtg tgtggcgacc aactcggcag gcacacgtta ctcagcccct 1261 gcgaacctgt atgtgcgagt gcgccgcgtg gctcctcgtt tctccatccc tcccagcagc 1321 caggaggtga tgccaggcgg cagcgtgaac ctgacatgcg tggcagtggg tgcacccatg 1381 ccctacgtga agtggatgat gggggccgag gagctcacca aggaggatga gatgccagtt 1441 ggccgcaacg tcctggagct cagcaatgtc gtacgctctg ccaactacac ctgtgtggcc 1501 atctcctcgc tgggcatgat cgaggccaca gcccaggtca cagtgaaagc tcttccaaag 1561 cctccgattg atcttgtggt gacagagaca actgccacca gtgtcaccct cacctgggac 1621 tctgggaact cggagcctgt aacctactat ggcatccagt accgcgcagc gggcacggag 1681 ggcccctttc aggaggtgga tggtgtggcc accacccgct acagcattgg cggcctcagc 1741 cctttctcgg aatatgcctt ccgcgtgctg gcggtgaaca gcatcgggcg agggccgccc 1801 agcgaggcag tgcgggcacg cacgggagaa caggcgccct ccagcccacc gcgccgcgtg 1861 caggcacgca tgctgagcgc cagcaccatg ctggtgcagt gggagcctcc cgaggagccc 1921 aacggcctgg tgcggggata ccgcgtctac tatactccgg actcccgccg ccccccgaac 1981 gcctggcaca agcacaacac cgacgcgggg ctcctcacga ccgtgggcag cctgctgcct 2041 ggcatcacct acagcctgcg cgtgcttgcc ttcaccgccg tgggcgatgg ccctcccagc 2101 cccaccatcc aggtcaagac gcagcaggga gtgcctgccc agcccgcgga cttccaggcc 2161 gaggtggagt cggacaccag gatccagctc tcgtggctgc tgccccctca ggagcggatc 2221 atcatgtatg aactggtgta ctgggcggca gaggacgaag accaacagca caaggtgacc 2281 ttcgacccaa cctcctccta cacactagag gacctgaagc ctgacacact ctaccgcttc 2341 cagctggctg cacgctcgga tatgggggtg ggcgtcttca cccccaccat tgaggcccgc 2401 acagcccagt ccaccccctc cgcccctccc cagaaggtga tgtgtgtgag catgggctcc 2461 accacggtcc gggtaagttg ggtcccgccg cctgccgaca gccgcaacgg cgttatcacc 2521 cagtactccg tggcctacga ggcggtggac ggcgaggacc gcgggcggca tgtggtggat 2581 ggcatcagcc gtgagcactc cagctgggac ctggtgggcc tggagaagtg gacggagtac 2641 cgggtgtggg tgcgggcaca cacagacgtg ggccccggcc ccgagagcag cccggtgctg 2701 gtgcgcaccg atgaggacgt gcccagcggg cctccgcgga aggtggaggt ggagccactg 2761 aactccactg ctgtgcatgt ctactggaag ctgcctgtcc ccagcaagca gcatggccag 2821 atccgcggct accaggtcac ctacgtgcgg ctggagaatg gcgagccccg tggactcccc 2881 atcatccaag acgtcatgct agccgaggcc cagtggcggc cagaggagtc cgaggactat 2941 gaaaccacta tcagcggcct gaccccggag accacctact ccgttactgt tgctgcctat 3001 accaccaagg gggatggtgc ccgcagcaag cccaaaattg tcactacaac aggtgcagtc 3061 ccaggccggc ccaccatgat gatcagcacc acggccatga acactgcgct gctccagtgg 3121 cacccaccca aggaactgcc tggcgagctg ctgggctacc ggctgcagta ctgccgggcc 3181 gacgaggcgc ggcccaacac catagatttc ggcaaggatg accagcactt cacagtcacc 3241 ggcctgcaca aggggaccac ctacatcttc cggcttgctg ccaagaaccg ggctggcttg 3301 ggtgaggagt tcgagaagga gatcaggacc cccgaggacc tgcccagcgg cttcccccaa 3361 aacctgcatg tgacaggact gaccacgtct accacagaac tggcctggga cccgccagtg 3421 ctggcggaga ggaacgggcg catcatcagc tacaccgtgg tgttccgaga catcaacagc 3481 caacaggagc tgcagaacat cacgacagac acccgcttta cccttactgg cctcaagcca 3541 gacaccactt acgacatcaa ggtccgcgca tggaccagca aaggctctgg cccactcagc 3601 cccagcatcc agtcccggac catgccggtg gagcaagtgt ttgccaagaa cttccgggtg 3661 gcggctgcaa tgaagacgtc tgtgctgctc agctgggagg ttcccgactc ctataagtca 3721 gctgtgccct ttaagattct gtacaatggg cagagtgtgg aggtggacgg gcactcgatg 3781 cggaagctga tcgcagacct gcagcccaac acagagtact cgtttgtgct gatgaaccgt 3841 ggcagcagcg cagggggcct gcagcacctg gtgtccatcc gcacagcccc cgacctcctg 3901 cctcacaagc cgctgcctgc ctctgcctac atagaggacg gccgcttcga tctctccatg 3961 ccccatgtgc aagacccctc gcttgtcagg tggttctaca ttgttgtggt gcccattgac 4021 cgtgtgggcg ggagcatgct gacgccaagg tggagcacac ccgaggaact ggagctggac 4081 gagcttctag aagccatcga gcaaggcgga gaggagcagc ggcggcggcg gcggcaggca 4141 gaacgtctga agccatatgt ggctgctcaa ctggatgtgc tcccggagac ctttaccttg 4201 ggggacaaga agaactaccg gggcttctac aaccggcccc tgtctccgga cttgagctac 4261 cagtgctttg tgcttgcctc cttgaaggaa cccatggacc agaagcgcta tgcctccagc 4321 ccctactcgg atgagatcgt ggtccaggtg acaccagccc agcagcagga ggagccggag 4381 atgctgtggg tgacgggtcc cgtgctggca gtcatcctca tcatcctcat tgtcatcgcc 4441 atcctcttgt tcaaaaggaa aaggacccac tctccgtcct ctaaggatga gcagtcgatc 4501 ggactgaagg actccttgct ggcccactcc tctgaccctg tggagatgcg gaggctcaac 4561 taccagaccc caggtatgcg agaccaccca cccatcccca tcaccgacct ggcggacaac 4621 atcgagcgcc tcaaagccaa cgatggcctc aagttctccc aggagtatga gtccatcgac 4681 cctggacagc agttcacgtg ggagaattca aacctggagg tgaacaagcc caagaaccgc 4741 tatgcgaatg tcatcgccta cgaccactct cgagtcatcc ttacctctat cgatggcgtc 4801 cccgggagtg actacatcaa tgccaactac atcgatggct accgcaagca gaatgcctac 4861 atcgccacgc agggccccct gcccgagacc atgggtgatt tctggaggat ggtgtgggaa 4921 cagcgcacgg ccactgtggt catgatgaca cggctggagg agaagtcccg ggtaaaatgt 4981 gatcagtact ggccagcccg tggcaccgag acctgtggcc ttattcaggt gaccctgttg 5041 gacacagtgg agctggccac atacactgtg cgcaccttcg cactccacaa gagtggctcc 5101 agtgagaagc gcgagctgcg tcagtttcag ttcatggcct ggccagacca tggagttcct 5161 gagtacccaa ctcccatcct ggccttccta cgacgggtca aggcctgcaa ccccctagac 5221 gcagggccca tggtggtgca ctgcagcgcg ggcgtgggcc gcaccggctg cttcatcgtg 5281 attgatgcca tgttggagcg gatgaagcac gagaagacgg tggacatcta tggccacgtg 5341 acctgcatgc gatcacagag gaactacatg gtgcagacgg aggaccagta cgtgttcatc 5401 catgaggcgc tgctggaggc tgccacgtgc ggccacacag aggtgcctgc ccgcaacctg 5461 tatgcccaca tccagaagct gggccaagtg cctccagggg agagtgtgac cgccatggag 5521 ctcgagttca agttgctggc cagctccaag gcccacacgt cccgcttcat cagcgccaac 5581 ctgccctgca acaagttcaa gaaccggctg gtgaacatca tgccctacga attgacccgt 5641 gtgtgtctgc agcccatccg tggtgtggag ggctctgact acatcaatgc cagcttcctg 5701 gatggttata gacagcagaa ggcctacata gctacacagg ggcctctggc agagagcacc 5761 gaggacttct ggcgcatgct atgggagcac aattccacca tcatcgtcat gctgaccaag 5821 cttcgggaga tgggcaggga gaaatgccac cagtactggc cagcagagcg ctctgctcgc 5881 taccagtact ttgttgttga cccgatggct gagtacaaca tgccccagta tatcctgcgt 5941 gagttcaagg tcacggatgc ccgggatggg cagtcaagga caatccggca gttccagttc 6001 acagactggc cagagcaggg cgtgcccaag acaggcgagg gattcattga cttcatcggg 6061 caggtgcata agaccaagga gcagtttgga caggatgggc ctatcacggt gcactgcagt 6121 gctggcgtgg gccgcaccgg ggtgttcatc actctgagca tcgtcctgga gcgcatgcgc 6181 tacgagggcg tggtcgacat gtttcagacc gtgaagaccc tgcgtacaca gcgtcctgcc 6241 atggtgcaga cagaggacca gtatcagctg tgctaccgtg cggccctgga gtacctcggc 6301 agctttgacc actatgcaac gGGAGGAGGG GGAAGCatgg tgagcaaggg cgaggaggat 6361 aacatggcct ctctcccagc gacacatgag ttacacatct ttggctccat caacggtgtg 6421 gactttgaca tggtgggtca gggcaccggc aatccaaatg atggttatga ggagttaaac 6481 ctgaagtcca ccaagggtga cctccagttc tccccctgga ttctggtccc tcatatcggg 6541 tatggcttcc atcagtacct gccctaccct gacgggatgt cgcctttcca ggccgccatg 6601 gtagatggct ccggatacca agtccatcgc acaatgcagt ttgaagatgg tgcctccctt 6661 actgttaact accgctacac ctacgaggga agccacatca aaggagaggc ccaggtgaag 6721 gggactggtt tccctgctga cggtcctgtg atgaccaact cgctgaccgc tgcggactgg 6781 tgcaggtcga agaagactta ccccaacgac aaaaccatca tcagtacctt taagtggagt 6841 tacaccactg gaaatggcaa gcgctaccgg agcactgcgc ggaccaccta cacctttgcc 6901 aagccaatgg cggctaacta tctgaagaac cagccgatgt acgtgttccg taagacggag 6961 ctcaagcact ccaagaccga gctcaacttc aaggagtggc aaaaggcctt taccgatgtg 7021 atgggcatgg acgagctgta caagtaggat ccaccggatc tagataactg atcataatca 7081 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga 7141 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg 7201 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt 7261 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat 7321 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc 7381 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt 7441 ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa 7501 accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg 7561 tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga 7621 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct 7681 agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat 7741 gcgccgctac agggcgcgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt 7801 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa 7861 atgcttcaat aatattgaaa aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg 7921 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 7981 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa 8041 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca 8101 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt 8161 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag 8221 gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg aggatcgttt 8281 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 8341 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 8401 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 8461 ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 8521 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 8581 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 8641 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 8701 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 8761 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc 8821 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 8881 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 8941 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 9001 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 9061 cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa gcgacgccca 9121 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 9181 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 9241 tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg gaaggaaccc 9301 gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg tttgttcata 9361 aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga ccccattggg 9421 gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg ggtgaaggcc 9481 cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt tactcatata 9541 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt 9601 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc 9661 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct 9721 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa 9781 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag 9841 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc 9901 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg 9961 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca 10021 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat 10081 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg 10141 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc 10201 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc 10261 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc 10321 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg 10381 ccatgcat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.