pCDH-CMV-3×FLAG-TMED3-EF1a-Cop

pCDH-CMV-3×FLAG-TMED3-EF1a-CopGFP-T2A-Puro plasmid

PVT93579

pCDH-CMV-3×FLAG-TMED3-EF1a-CopGFP-T2A-Puro plasmid Information

Plasmid name: pCDH-CMV-3×FLAG-TMED3(human)-EF1a-CopGFP-T2A-Puro
Alias: NM_007364.4;C15orf22; P24B; p24g4; p24gamma4; p26
Gene lenght: 651bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: EF1a-R
Copy number: High
Induction:

pCDH-CMV-3×FLAG-TMED3-EF1a-CopGFP-T2A-Puro plasmid Description

pCDH-CMV-3×FLAG-TMED3-EF1a-CopGFP-T2A-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pCDH-CMV-3×FLAG-TMED3-EF1a-CopGFP-T2A-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt 1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc 1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga 1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac 1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga 2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg 2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg 2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac 2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagagc 2281 caccatgGAC TACAAGGATG ACGATGACAA GGATTACAAA GACGACGATG ATAAGGACTA 2341 TAAGGATGAT GACGACAAAg aattcatggg cagcactgtc ccgcgctccg cctccgtgct 2401 gcttctgctg ctgctcctgc gccgggccga gcagccctgc ggggccgagc tcaccttcga 2461 gctgccggac aacgccaagc agtgcttcca cgaggaggtg gagcagggcg tgaagttctc 2521 cctggattac caggtcatca ctggaggcca ctacgatgtt gactgctatg tagaggaccc 2581 ccaggggaac accatctaca gagaaacgaa gaagcagtac gacagcttca cgtaccgggc 2641 tgaagtcaag ggcgtttatc agttttgctt cagtaatgag ttttccacct tctctcacaa 2701 gaccgtctac tttgactttc aagtgggcga tgagcctccc attctcccag acatggggaa 2761 cagggtcaca gctctcaccc agatggagtc cgcctgcgtg accatccatg aggctctgaa 2821 aacggtgatt gactcccaga cgcattaccg gctgcgggag gcccaggacc gggcccgagc 2881 agaagacctt aatagccgag tctcttactg gtctgttggc gagacgattg ccctgttcgt 2941 ggtcagcttc agtcaggtgc tactgttgaa aagcttcttc acagaaaaac gacccatcag 3001 cagggcagtc cactccTAGg gatccgcggc cgcgaaggat ctgcgatcgc tccggtgccc 3061 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca 3121 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact 3181 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga 3241 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca 3301 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg 3361 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa 3421 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc 3481 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg 3541 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac gctagacgcc accatggaga 3601 gcgacgagag cggcctgccc gccatggaga tcgagtgccg catcaccggc accctgaacg 3661 gcgtggagtt cgagctggtg ggcggcggag agggcacccc caagcagggc cgcatgacca 3721 acaagatgaa gagcaccaaa ggcgccctga ccttcagccc ctacctgctg agccacgtga 3781 tgggctacgg cttctaccac ttcggcacct accccagcgg ctacgagaac cccttcctgc 3841 acgccatcaa caacggcggc tacaccaaca cccgcatcga gaagtacgag gacggcggcg 3901 tgctgcacgt gagcttcagc taccgctacg aggccggccg cgtgatcggc gacttcaagg 3961 tggtgggcac cggcttcccc gaggacagcg tgatcttcac cgacaagatc atccgcagca 4021 acgccaccgt ggagcacctg caccccatgg gcgataacgt gctggtgggc agcttcgccc 4081 gcaccttcag cctgcgcgac ggcggctact acagcttcgt ggtggacagc cacatgcact 4141 tcaagagcgc catccacccc agcatcctgc agaacggggg ccccatgttc gccttccgcc 4201 gcgtggagga gctgcacagc aacaccgagc tgggcatcgt ggagtaccag cacgccttca 4261 agacccccat cgccttcgcc agatcccgcg ctcagtcgtc caattctgcc gtggacggca 4321 ccgccggacc cggctccacc ggatctcgcg agggcagagg aagtcttcta acatgcggtg 4381 acgtggagga gaatcccggc cctatgaccg agtacaagcc cacggtgcgc ctcgccaccc 4441 gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca 4501 cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct 4561 tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg 4621 tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc 4681 cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc 4741 tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg 4801 accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc 4861 gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc 4921 ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca 4981 tgacccgcaa gcccggtgcc tgaaatcaac ctctggatta caaaatttgt gaaagattga 5041 ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt 5101 tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt 5161 tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg 5221 tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg 5281 ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc 5341 gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc 5401 tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct 5461 tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg 5521 ctctgcggcc tcttccgcgt ctccgccttc gccctcagac gagtcggatc tccctttggc 5581 cgcctccccg cctggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc 5641 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga aaataagatc 5701 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg 5761 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag 5821 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag 5881 tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg 5941 caaagaaatg aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca 6001 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt 6061 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct 6121 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc 6181 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg 6241 aggcctagac ttttgcagag acggcccaaa ttcgtaatca tggtcatagc tgtttcctgt 6301 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 6361 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 6421 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 6481 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 6541 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 6601 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 6661 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 6721 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 6781 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 6841 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 6901 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 6961 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 7021 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 7081 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 7141 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 7201 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 7261 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 7321 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 7381 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 7441 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 7501 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 7561 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 7621 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 7681 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 7741 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 7801 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 7861 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 7921 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 7981 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 8041 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 8101 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 8161 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 8221 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 8281 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 8341 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 8401 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 8461 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga 8521 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc 8581 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg 8641 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 8701 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct 8761 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 8821 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 8881 ttgtaaaacg acggccagtg ccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.