pCDH-CMV-HLA-B-EF1-CopGFP-T2A-

pCDH-CMV-HLA-B-EF1-CopGFP-T2A-Puro plasmid

PVT93560

pCDH-CMV-HLA-B-EF1-CopGFP-T2A-Puro plasmid Information

Plasmid name: pCDH-CMV-HLA-B(human)-EF1-CopGFP-T2A-Puro
Alias: NM_005514.8;AS; B-4901; HLAB
Gene lenght: 1089bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCDH-CMV-HLA-B-EF1-CopGFP-T2A-Puro plasmid Description

pCDH-CMV-HLA-B-EF1-CopGFP-T2A-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pCDH-CMV-HLA-B-EF1-CopGFP-T2A-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc gaattcGCCA CCatgctggt catggcgccc cgaaccgtcc tcctgctgct 2341 ctcggcggcc ctggccctga ccgagacctg ggccggctcc cactccatga ggtatttcta 2401 cacctccgtg tcccggcccg gccgcgggga gccccgcttc atctcagtgg gctacgtgga 2461 cgacacccag ttcgtgaggt tcgacagcga cgccgcgagt ccgagagagg agccgcgggc 2521 gccgtggata gagcaggagg ggccggagta ttgggaccgg aacacacaga tctacaaggc 2581 ccaggcacag actgaccgag agagcctgcg gaacctgcgc ggctactaca accagagcga 2641 ggccgggtct cacaccctcc agagcatgta cggctgcgac gtggggccgg acgggcgcct 2701 cctccgcggg catgaccagt acgcctacga cggcaaggat tacatcgccc tgaacgagga 2761 cctgcgctcc tggaccgccg cggacacggc ggctcagatc acccagcgca agtgggaggc 2821 ggcccgtgag gcggagcagc ggagagccta cctggagggc gagtgcgtgg agtggctccg 2881 cagatacctg gagaacggga aggacaagct ggagcgcgct gaccccccaa agacacacgt 2941 gacccaccac cccatctctg accatgaggc caccctgagg tgctgggccc tgggtttcta 3001 ccctgcggag atcacactga cctggcagcg ggatggcgag gaccaaactc aggacactga 3061 gcttgtggag accagaccag caggagatag aaccttccag aagtgggcag ctgtggtggt 3121 gccttctgga gaagagcaga gatacacatg ccatgtacag catgaggggc tgccgaagcc 3181 cctcaccctg agatgggagc cgtcttccca gtccaccgtc cccatcgtgg gcattgttgc 3241 tggcctggct gtcctagcag ttgtggtcat cggagctgtg gtcgctgctg tgatgtgtag 3301 gaggaagagt tcaggtggaa aaggagggag ctactctcag gctgcgtgca gcgacagtgc 3361 ccagggctct gatgtgtctc tcacagcttg aggatccgcg gccgcGaagg atctgcgatc 3421 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg 3481 gaggggtcgg caattgaacg ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg 3541 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag 3601 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag ctgaagcttc 3661 gaggggctcg catctctcct tcacgcgccc gccgccctac ctgaggccgc catccacgcc 3721 ggttgagtcg cgttctgccg cctcccgcct gtggtgcctc ctgaactgcg tccgccgtct 3781 aggtaagttt aaagctcagg tcgagaccgg gcctttgtcc ggcgctccct tggagcctac 3841 ctagactcag ccggctctcc acgctttgcc tgaccctgct tgctcaactc tacgtctttg 3901 tttcgttttc tgttctgcgc cgttacagat ccaagctgtg accggcgcct acgctagacg 3961 ccaccatgga gagcgacgag agcggcctgc ccgccatgga gatcgagtgc cgcatcaccg 4021 gcaccctgaa cggcgtggag ttcgagctgg tgggcggcgg agagggcacc cccaagcagg 4081 gccgcatgac caacaagatg aagagcacca aaggcgccct gaccttcagc ccctacctgc 4141 tgagccacgt gatgggctac ggcttctacc acttcggcac ctaccccagc ggctacgaga 4201 accccttcct gcacgccatc aacaacggcg gctacaccaa cacccgcatc gagaagtacg 4261 aggacggcgg cgtgctgcac gtgagcttca gctaccgcta cgaggccggc cgcgtgatcg 4321 gcgacttcaa ggtggtgggc accggcttcc ccgaggacag cgtgatcttc accgacaaga 4381 tcatccgcag caacgccacc gtggagcacc tgcaccccat gggcgataac gtgctggtgg 4441 gcagcttcgc ccgcaccttc agcctgcgcg acggcggcta ctacagcttc gtggtggaca 4501 gccacatgca cttcaagagc gccatccacc ccagcatcct gcagaacggg ggccccatgt 4561 tcgccttccg ccgcgtggag gagctgcaca gcaacaccga gctgggcatc gtggagtacc 4621 agcacgcctt caagaccccc atcgccttcg ccagatcccg cgctcagtcg tccaattctg 4681 ccgtggacgg caccgccgga cccggctcca ccggatctcg cgagggcaga ggaagtcttc 4741 taacatgcgg tgacgtggag gagaatcccg gccctatgac cgagtacaag cccacggtgc 4801 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg 4861 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc 4921 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg 4981 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg 5041 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga 5101 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg 5161 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg 5221 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc 5281 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc 5341 gcacctggtg catgacccgc aagcccggtg cctgaaatca acctctggat tacaaaattt 5401 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg 5461 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt 5521 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg 5581 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc 5641 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg 5701 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt 5761 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc 5821 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg 5881 gcctgctgcc ggctctgcgg cctcttccgc gtctccgcct tcgccctcag acgagtcgga 5941 tctccctttg gccgcctccc cgcctggtac ctttaagacc aatgacttac aaggcagctg 6001 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac 6061 gaaaataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct 6121 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag 6181 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac 6241 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt 6301 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt 6361 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac 6421 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc 6481 tatcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt 6541 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg 6601 aggctttttt ggaggcctag acttttgcag agacggccca aattcgtaat catggtcata 6661 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag 6721 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg 6781 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca 6841 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 6901 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 6961 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 7021 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 7081 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 7141 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 7201 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 7261 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 7321 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 7381 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 7441 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac 7501 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc 7561 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat 7621 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc 7681 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt 7741 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta 7801 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct 7861 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg 7921 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga 7981 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt 8041 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt 8101 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt 8161 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat 8221 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc 8281 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc 8341 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat 8401 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag 8461 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 8521 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 8581 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 8641 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 8701 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 8761 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac 8821 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc 8881 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc 8941 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg 9001 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca 9061 tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc 9121 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg 9181 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc 9241 ccagtcacga cgttgtaaaa cgacggccag tgccaagctg

//

Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.