pCMV-PAGR1-EGFP plasmid

pCMV-PAGR1-EGFP plasmid

PVT93502

pCMV-PAGR1-EGFP plasmid Information

Plasmid name: pCMV-PAGR1(human)-EGFP
Alias: NM_024516.4;C16orf53; GAS; PA1
Gene lenght: 765bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: pcaggs-F
Reverse primer:
Copy number: High
Induction:

pCMV-PAGR1-EGFP plasmid Description

pCMV-PAGR1-EGFP is a plasmid suitable for protein expression in mammalian cells.

pCMV-PAGR1-EGFP plasmid Sequence

1 tagttattaa ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg 61 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc 121 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt 181 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc 241 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 301 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 361 ctattaccat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct 421 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg 481 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga 541 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg 601 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg gggagtcgct 661 gcgacgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc cgccccggct 721 ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg 781 taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg aaagccttga 841 ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggggtgcgt gcgtgtgtgt 901 gtgcgtgggg agcgccgcgt gcggctccgc gctgcccggc ggctgtgagc gctgcgggcg 961 cggcgcgggg ctttgtgcgc tccgcagtgt gcgcgagggg agcgcggccg ggggcggtgc 1021 cccgcggtgc ggggggggct gcgaggggaa caaaggctgc gtgcggggtg tgtgcgtggg 1081 ggggtgagca gggggtgtgg gcgcgtcggt cgggctgcaa ccccccctgc acccccctcc 1141 ccgagttgct gagcacggcc cggcttcggg tgcggggctc cgtacggggc gtggcgcggg 1201 gctcgccgtg ccgggcgggg ggtggcggca ggtgggggtg ccgggcgggg cggggccgcc 1261 tcgggccggg gagggctcgg gggaggggcg cggcggcccc cggagcgccg gcggctgtcg 1321 aggcgcggcg agccgcagcc attgcctttt atggtaatcg tgcgagaggg cgcagggact 1381 tcctttgtcc caaatctgtg cggagccgaa atctgggagg cgccgccgca ccccctctag 1441 cgggcgcggg gcgaagcggt gcggcgccgg caggaaggaa atgggcgggg agggccttcg 1501 tgcgtcgccg cgccgccgtc cccttctccc tctccagcct cggggctgtc cgcgggggga 1561 cggctgcctt cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg 1621 ctctagagcc tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa 1681 cgtgctggtt attgtgctgt ctcatcattt tggcaaagga tccctcgagg ccaccatgtc 1741 ccttgctcgg ggccatggag acactgcggc cagtacggcg gcgcctctgt ctgaagaagg 1801 ggaagtgacc tccggcctcc aggctctggc cgtggaggat accggaggcc cctctgcctc 1861 ggccggtaag gccgaggacg agggggaagg aggccgagag gagaccgagc gtgaggggtc 1921 cgggggcgag gaggcgcagg gagaagtccc cagcgctggg ggagaagagc ctgccgagga 1981 ggactccgag gactggtgcg tgccctgcag cgacgaggag gtggagctgc ctgcggatgg 2041 gcagccctgg atgcccccgc cctccgaaat ccagcggctc tatgaactgc tggctgccca 2101 cggtactctg gagctgcaag ccgagatcct gccccgccgg cctcccacgc cggaggccca 2161 gagcgaagag gagagatccg atgaggagcc ggaggccaaa gaagaggaag aggaaaaacc 2221 acacatgccc acggaatttg attttgatga tgagccagtg acaccaaagg actccctgat 2281 tgaccggaga cgcaccccag gaagctcagc ccggagccag aaacgggagg cccgcctgga 2341 caaggtgctg tcggacatga agagacacaa gaagctggag gagcagatcc ttcgtaccgg 2401 gagggacctc ttcagcctgg actcggagga ccccagcccc gccagccccc cactccgatc 2461 ctccgggagt agtctcttcc ctcggcagcg gaaatacACC GGTATGGTGA GCAAGGGCGA 2521 GGAGCTGTTC ACCGGGGTGG TGCCCATCCT GGTCGAGCTG GACGGCGACG TAAACGGCCA 2581 CAAGTTCAGC GTGTCCGGCG AGGGCGAGGG CGATGCCACC TACGGCAAGC TGACCCTGAA 2641 GTTCATCTGC ACCACCGGCA AGCTGCCCGT GCCCTGGCCC ACCCTCGTGA CCACCCTGAC 2701 CTACGGCGTG CAGTGCTTCA GCCGCTACCC CGACCACATG AAGCAGCACG ACTTCTTCAA 2761 GTCCGCCATG CCCGAAGGCT ACGTCCAGGA GCGCACCATC TTCTTCAAGG ACGACGGCAA 2821 CTACAAGACC CGCGCCGAGG TGAAGTTCGA GGGCGACACC CTGGTGAACC GCATCGAGCT 2881 GAAGGGCATC GACTTCAAGG AGGACGGCAA CATCCTGGGG CACAAGCTGG AGTACAACTA 2941 CAACAGCCAC AACGTCTATA TCATGGCCGA CAAGCAGAAG AACGGCATCA AGGTGAACTT 3001 CAAGATCCGC CACAACATCG AGGACGGCAG CGTGCAGCTC GCCGACCACT ACCAGCAGAA 3061 CACCCCCATC GGCGACGGCC CCGTGCTGCT GCCCGACAAC CACTACCTGA GCACCCAGTC 3121 CGCCCTGAGC AAAGACCCCA ACGAGAAGCG CGATCACATG GTCCTGCTGG AGTTCGTGAC 3181 CGCCGCCGGG ATCACTCTCG GCATGGACGA GCTGTACAAG taagcggccg cgactctaga 3241 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc 3301 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag 3361 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 3421 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtaaattgta 3481 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac 3541 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg 3601 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa 3661 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt 3721 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt 3781 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga 3841 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc 3901 gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga 3961 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 4021 ccctgataaa tgcttcaata atattgaaaa aggaagagtc ctgaggcgga aagaaccagc 4081 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta 4141 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag 4201 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa 4261 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac 4321 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt 4381 agtgaggagg cttttttgga ggcctaggct tttgcaaaga tcgatcaaga gacaggatga 4441 ggatcgtttc gcatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca 4501 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat 4561 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag 4621 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc 4681 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct 4741 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca 4801 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt 4861 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat 4921 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt 4981 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta 5041 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga 5101 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt 5161 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc 5221 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct 5281 gagataggtg cctcactgat taagcattgg taagcgggac tctggggttc gaaatgaccg 5341 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa 5401 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc 5461 tcatgctgga gttcttcgcc caccctaggg ggaggctaac tgaaacacgg aaggagacaa 5521 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacggtgttg 5581 ggtcgtttgt tcataaacgc ggggttcggt cccagggctg gcactctgtc gataccccac 5641 cgagacccca ttggggccaa tacgcccgcg tttcttcctt ttccccaccc caccccccaa 5701 gttcgggtga aggcccaggg ctcgcagcca acgtcggggc ggcaggccct gccatagcct 5761 caggttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct 5821 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc 5881 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc 5941 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg 6001 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa 6061 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc 6121 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt 6181 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa 6241 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc 6301 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc 6361 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct 6421 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat 6481 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc 6541 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg 6601 ataaccgtat taccgccatg cat

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.