pCMV-Ano4-3×HA-Neo plasmid

pCMV-Ano4-3×HA-Neo plasmid

PVT93499

pCMV-Ano4-3×HA-Neo plasmid Information

Plasmid name: pCMV-Ano4(mouse)-3×HA-Neo
Alias: NM_001277188.1;A330096O15Rik; Gm65; Tmem16d
Gene lenght: 2865bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-Ano4-3×HA-Neo plasmid Description

pCMV-Ano4-3×HA-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-Ano4-3×HA-Neo plasmid Sequence

1 GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATCTG CTCCCTGCTT 61 GTGTGTTGGA GGTCGCTGAG TAGTGCGCGA GCAAAATTTA AGCTACAACA AGGCAAGGCT 121 TGACCGACAA TTGCATGAAG AATCTGCTTA GGGTTAGGCG TTTTGCGCTG CTTCGCGATG 181 TACGGGCCAG ATATACGCGT TGACATTGAT TATTGACTAG TTATTAATAG TAATCAATTA 241 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 301 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 361 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 421 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 481 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 541 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 601 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 661 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 721 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 781 GAGCTCTCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC 841 ACTATAGGGA GACCCAAGCT GGCTAGCGTT TAAACTTAAG CTTGGTACCG AGCTCGGATC 901 Cgccaccatg gaggccagct cttctggaat cactaatgga aaaaataaag ttttccacgc 961 agaaggtgga ctggatttgc aaagccatca gctggatatg caaatactgc ccgacggccc 1021 aaagagcgat gtggactttt cagagattct taatgcaata caagaaatgg ccaaggatgt 1081 caatattctt tttgacgaac ttgaggctgt caacagccct tgcaaagatg atgattctct 1141 gcttcaccct ggaaatctga cgagcacttc ggaggacacc agcaggctag aagcaggggg 1201 agagacagtg cgagaacgaa acaaatccaa tggactttac tttagagacg gaaaatgtcg 1261 aattgactac attcttgtgt acagaaaatc caaccctcag actgaaaaga gagaagtgtt 1321 tgaaagaaat attagagcag aaggattaca aatggagaaa gagtcctctc taataaatag 1381 tgacattatc tttgtgaagt tgcatgcccc atgggaagtc cttggaagat atgcagagca 1441 aatgaatgta cggatgcctt tcaggagaaa aatctattac ctgccccgcc ggtacaagtt 1501 catgagcagg atcgataaac aaataagcag gttccggaga tggttaccta agaagccaat 1561 gaggctggac aaggagacac tgccagacct ggaagagaat gactgctaca ctgccccttt 1621 cagccaacaa aggatccatc acttcatcat acacaacaaa gacactttct tcaacaatgc 1681 cacaagaagc agaatcgtgc atcatatttt acaaagaata aagtacgagg aaggaaagaa 1741 taaaatcggt ctgaaccgct tgcttaccaa tggctcctat gaagctgcct ttcccctgca 1801 tgagggaagt tacaggagta aaaactccat taaaacccat ggagctgtaa accaccgaca 1861 tctactgtat gagtgctggg cctcctgggg cgtgtggtac aagtaccagc ctttggatct 1921 tgtcaggcga tattttggag agaagattgg actgtatttc gcctggctgg gctggtacac 1981 aggcatgctc ttccccgcag ccttcattgg gttgtttgtc ttcttgtatg gagtcaccac 2041 tctggatcac tgccaagtca gtaaagaagt ctgccaagca acagatatca tcatgtgtcc 2101 tgtatgtgac aaatactgtc cattcatgag gctttcagac agctgtgtct atgccaaggt 2161 gacccacctt tttgacaatg gggccactgt gttctttgct gtgttcatgg cagtctgggc 2221 tactgttttc ctggaattct ggaagagacg ccgagcagtg atcgcttatg actgggatct 2281 gattgactgg gaagaagagg aggaagaaat acgaccccag tttgaagcca agtattccaa 2341 gaaagagcgg atgaatccca tttcaggaaa gccagaacct tatcaagcat ttactgataa 2401 atgcagcaga cttatcgttt ctgcatcagg aatattcttc atgatctgcg tggtgatcgc 2461 tgctgtgttc gggatcgtca tttacagggt ggtgactgtg agcactttcg ctgcctttaa 2521 gtgggcatta atcaggaata actctcaggt tgcaaccaca gggactgcag tatgcatcaa 2581 cttttgtatc attatgctgc tgaatgtgct ctatgaaaaa gtcgcactcc ttctaacaaa 2641 tctggaacag cctcgcacag aatccgagtg ggagaacagc tttaccttga aaatgtttct 2701 ttttcagttt gtcaatctga acagctccac tttttacatc gcattcttcc tcggaagatt 2761 tacaggacac ccaggtgcct acttgagatt gataaacagg tggaggctag aagagtgcca 2821 ccctagcgga tgccttattg atctctgcat gcaaatgggt attataatgg tgctaaagca 2881 gacctggaat aatttcatgg aacttggcta cccgttaatt caaaattggt ggactagaag 2941 aaaagtccga caagaacatg ggactgaaag gaaaataaat ttcccacagt gggaaaagga 3001 ctacaatctt cagccgatga atgcctatgg actcttcgat gaatacctgg aaatgatcct 3061 tcagttcgga ttcacaacga tctttgtggc agcttttcct ctagcaccgc ttctggcctt 3121 actgaataac atcattgaaa tccgacttga tgcttataag tttgttactc agtggagaag 3181 gcctttggct tcgagagcca aagacatagg aatttggtat ggaattctcg aaggcattgg 3241 gattctgtct gttattacaa atgcatttgt catagcaata acatccgact tcatccctcg 3301 cttagtatat gcttataaat atggaccttg tgcaggccaa ggagaagctg ggcaaaagtg 3361 catggttggc tatgtgaatg ccagcttgtc tgtgttccga atttctgact ttgaaaatcg 3421 gtcggagcca gagtctgatg gcagtgagtt ctcaggaacc cctctgaagt actgcagata 3481 tcgagactac cgggaccctc ctcactcttt ggcaccctat ggctacacac tgcagttctg 3541 gcatgttctc gcagctcggc tggcttttat cattgtgttt gagcacctcg tgttttgtat 3601 aaagcacctc atttcctatc tgataccaga cctcccgaaa gatctaaggg accggatgag 3661 gagagagaag tacttgatcc aggagatgat gtatgaggct gagctggagc gcctccagaa 3721 agaacggaag gagaggaaga agaacggaaa agcacatcac aatgagtggc caCTCGAGTA 3781 CCCATACGAC GTACCTGATT ATGCAACTTA TCCTTATGAT GTACCTGATT ATGCTAGCTA 3841 TCCTTATGAT GTCCCAGACT ACGCAtgaGG GCCCGTTTAA ACCCGCTGAT CAGCCTCGAC 3901 TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT 3961 GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT 4021 GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG 4081 GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG 4141 AACCAGCTGG GGCTCTAGGG GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC 4201 GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC 4261 TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA 4321 TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT 4381 TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT 4441 GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA 4501 CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT 4561 AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG 4621 TTAGGGTGTG GAAAGTCCCC AGGCTCCCCA GCAGGCAGAA GTATGCAAAG CATGCATCTC 4681 AATTAGTCAG CAACCAGGTG TGGAAAGTCC CCAGGCTCCC CAGCAGGCAG AAGTATGCAA 4741 AGCATGCATC TCAATTAGTC AGCAACCATA GTCCCGCCCC TAACTCCGCC CATCCCGCCC 4801 CTAACTCCGC CCAGTTCCGC CCATTCTCCG CCCCATGGCT GACTAATTTT TTTTATTTAT 4861 GCAGAGGCCG AGGCCGCCTC TGCCTCTGAG CTATTCCAGA AGTAGTGAGG AGGCTTTTTT 4921 GGAGGCCTAG GCTTTTGCAA AAAGCTCCCG GGAGCTTGTA TATCCATTTT CGGATCTGAT 4981 CAAGAGACAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA CGCAGGTTCT 5041 CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC AATCGGCTGC 5101 TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT TGTCAAGACC 5161 GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC GTGGCTGGCC 5221 ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG AAGGGACTGG 5281 CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC TCCTGCCGAG 5341 AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC GGCTACCTGC 5401 CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT GGAAGCCGGT 5461 CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC CGAACTGTTC 5521 GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA TGGCGATGCC 5581 TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA CTGTGGCCGG 5641 CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT TGCTGAAGAG 5701 CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC TCCCGATTCG 5761 CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT CTGGGGTTCG 5821 AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC ACCGCCGCCT 5881 TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG ATCCTCCAGC 5941 GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA GCTTATAATG 6001 GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT TCACTGCATT 6061 CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA CCGTCGACCT 6121 CTAGCTAGAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT TGTTATCCGC 6181 TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG GGTGCCTAAT 6241 GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG TCGGGAAACC 6301 TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT TTGCGTATTG 6361 GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG CTGCGGCGAG 6421 CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG 6481 GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 6541 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 6601 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 6661 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 6721 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 6781 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 6841 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 6901 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 6961 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 7021 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 7081 GCGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 7141 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 7201 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 7261 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 7321 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 7381 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 7441 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 7501 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 7561 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 7621 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 7681 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 7741 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 7801 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 7861 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 7921 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 7981 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 8041 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 8101 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 8161 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 8221 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 8281 GAAAAGTGCC ACCTGACGTC GACGGATCGG GAGATCTCCC GATCCCCTAT GGT

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.