pLV2-CMV-Slc9a2-Fluc-Puro plas

pLV2-CMV-Slc9a2-Fluc-Puro plasmid

PVT93452

pLV2-CMV-Slc9a2-Fluc-Puro plasmid Information

Plasmid name: pLV2-CMV-Slc9a2(mouse)-Fluc-Puro
Alias: NM_001033289.2;2210416H12Rik; 4932415O19Rik; NHE-2; NHE2
Gene lenght: 2442bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Mouse
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Fluc
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV2-CMV-Slc9a2-Fluc-Puro plasmid Description

pLV2-CMV-Slc9a2-Fluc-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV2-CMV-Slc9a2-Fluc-Puro plasmid Sequence

1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca 61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac 121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca 181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg 241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag 301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg 361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat 421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga 481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct 541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc 601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag 661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg 721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga 781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg 841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg 901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct 961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat 1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca 1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc 1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag 1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc 1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg 1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc 1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc 1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct 1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa 1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca 1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt 1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt 1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta 1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt 1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct 1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga 1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag 2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac 2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg 2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa 2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg 2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt 2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg 2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc 2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc 2521 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca 2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta 2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa 2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc 2761 tccatagaag acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag 2821 GCCACCatgg gcccccgggg caccgcgcac agcgtgcggg cgccactgtc ttggctgctg 2881 ctgctgctgc tgtctctgca ggtggcagtg ccggcgggcg cgctggccga gaccctgctg 2941 gacgcaccca gggccagggg tgccagctcc aacccgccaa gtcctgccag tgtggtggcc 3001 ccgggaacga cgccgttcga ggaaagtcgg ttgcccgtgt tcaccctgga ttacccccac 3061 gtgcaaatcc ctttcgagat cactttgtgg atcctactgg cctccttggc caagattggt 3121 tttcatctct atcacaagtt gcccacaatc gtgccggaga gctgccttct cataatggtc 3181 gggcttctac tgggtgggat tatcttcggt gttgatgaga agtctccccc ggcaatgaag 3241 acagatgtgt ttttcctata cctcctgcca cccatcgttc tggatgctgg ctacttcatg 3301 ccaactcgcc ccttctttga gaacttcggc accattttct ggtatgctgt ggtaggcaca 3361 ctgtggaatt ccattggcat tggggtgtct ttgtttggca tctgccagat tgaggcgttt 3421 ggcctcagtg acatcaccct gctccagaac ctgctcttcg gcagcctgat ttcagctgtg 3481 gaccccgtgg ctgtgcttgc tgtctttgag aacattcacg tcaatgaaca gctctatatc 3541 ctggtttttg gcgagtctct gctcaacgat gcagtcacag tggtcctgta caacttgttc 3601 aagtccttct gccagatgaa aaccatccag accgtagacg tgtttgctgg catcgctaac 3661 ttcttcgtcg tgggtattgg cggggtgctg attggcatcc tcctgggatt catagcagcg 3721 tttaccaccc gtttcacaca caacatccgg gtcattgagc cactctttgt cttcttgtac 3781 agctacttgt cctacatcac tgctgaaatg ttccaccttt caggcatcat ggcgatcaca 3841 gcgtgtgcca tgacaatgaa taagtacgtg gaagagaacg tgtctcagaa gtcctacaca 3901 accatcaagt acttcatgaa gatgctgagc agcgtgagcg agaccctgat cttcatcttc 3961 atgggcgtgt ccactgttgg gaagaaccac gagtggaact gggctttcgt ctgctttacc 4021 ctggccttct gcctgatctg gcgagcactg ggcgtctttg tcctgactca ggtgattaac 4081 tggttccgga ccatcccgct aaccttcaag gaccagttca tcattgccta cggagggctc 4141 cgtggcgcca tctgctttgc actggtgttt ctcctccctg ctactgtgtt tccccgaaag 4201 aagctgttca tcacggctgc tattgtcgtt atattcttca ctgtcttcat cctgggtata 4261 accatccggc cactggtgga atttcttgat gttaagagat ccaataagaa gcagcaagct 4321 gtcagtgaag agatccactg tcggtttttt gatcatgtga agactgggat tgaagatgtg 4381 tgtggacact ggggtcacaa cttctggaga gacaagttta agaagtttga tgacaaatac 4441 cttcggaagc ttctgattcg ggaaaaccaa cccaagtcca gtattgtgtc cttatataaa 4501 aagcttgaga taaagcatgc cattgaaatg gcagagacag ggatgataag cactgtcccc 4561 tcatttgcat ctctcaatga ctgccgtgaa gagaaaataa ggaagctcac gccaggcgaa 4621 atggatgaaa ttagagagat attatcacgg aatctctatc aaatccgtca gcggaccttg 4681 tcctataaca gacacaacct gacggcagac acaagcgaga ggcaagccaa ggagatcctg 4741 attcgcaggc ggcacagtct tcgggaaagt ctcaggaaag acaacagctc aaaccgcgag 4801 cgcagggctt ccacttcaac ctcccgatat ttatccttac ctaagaacac aaagcttcca 4861 gagaagctgc agaagaagaa gaaggtttca aatgcagatg gaaatagcag cgactcggac 4921 ccagatgttg gaaccactgt gctcaatttg cagcccagga ccaggcgctt cttgccagat 4981 cagttctcaa agaaagcctc ccaggcctac aaaatggaat ggaagaacga ggtggatgtg 5041 ggttctgtgc gagtgccccc cagcgtctct ccagcgcccc gcagtaaaga cgggggcacc 5101 caaacaccgg ggttgctgag gcagcccctg ctctccaaag accaacggtt tggccggggt 5161 agggaagaca gtttgactga agacgttcca cccaagcccc caccaaggtt ggtgcgacga 5221 gcgtcagaac ccgggaacag gaaaggccgc cttggcaatg aaaagccagg atccATGGCC 5281 ACCAACTTCA GCCTGCTGAA GCAGGCCGGC GACGTGGAGG AGAACCCCGG CCCCGAAGAT 5341 GCCAAAAACA TTAAGAAGGG CCCAGCGCCA TTCTACCCAC TCGAAGACGG GACCGCCGGC 5401 GAGCAGCTGC ACAAAGCCAT GAAGCGCTAC GCCCTGGTGC CCGGCACCAT CGCCTTTACC 5461 GACGCACATA TCGAGGTGGA CATTACCTAC GCCGAGTACT TCGAGATGAG CGTTCGGCTG 5521 GCAGAAGCTA TGAAGCGCTA TGGGCTGAAT ACAAACCATC GGATCGTGGT GTGCAGCGAG 5581 AATAGCTTGC AGTTCTTCAT GCCCGTGTTG GGTGCCCTGT TCATCGGTGT GGCTGTGGCC 5641 CCAGCTAACG ACATCTACAA CGAGCGCGAG CTGCTGAACA GCATGGGCAT CAGCCAGCCC 5701 ACCGTCGTAT TCGTGAGCAA GAAAGGGCTG CAAAAGATCC TCAACGTGCA AAAGAAGCTA 5761 CCGATCATAC AAAAGATCAT CATCATGGAT AGCAAGACCG ACTACCAGGG CTTCCAAAGC 5821 ATGTACACCT TCGTGACTTC CCATTTGCCA CCCGGCTTCA ACGAGTACGA CTTCGTGCCC 5881 GAGAGCTTCG ACCGGGACAA AACCATCGCC CTGATCATGA ACAGTAGTGG CAGTACCGGA 5941 TTGCCCAAGG GCGTAGCCCT ACCGCACCGC ACCGCTTGTG TCCGATTCAG TCATGCCCGC 6001 GACCCCATCT TCGGCAACCA GATCATCCCC GACACCGCTA TCCTCAGCGT GGTGCCATTT 6061 CACCACGGCT TCGGCATGTT CACCACGCTG GGCTACTTGA TCTGCGGCTT TCGGGTCGTG 6121 CTCATGTACC GCTTCGAGGA GGAGCTATTC TTGCGCAGCT TGCAAGACTA TAAGATTCAA 6181 TCTGCCCTGC TGGTGCCCAC ACTATTTAGC TTCTTCGCTA AGAGCACTCT CATCGACAAG 6241 TACGACCTAA GCAACTTGCA CGAGATCGCC AGCGGCGGGG CGCCGCTCAG CAAGGAGGTA 6301 GGTGAGGCCG TGGCCAAACG CTTCCACCTA CCAGGCATCC GCCAGGGCTA CGGCCTGACA 6361 GAAACAACCA GCGCCATTCT GATCACCCCC GAAGGGGACG ACAAGCCTGG CGCAGTAGGC 6421 AAGGTGGTGC CCTTCTTCGA GGCTAAGGTG GTGGACTTGG ACACCGGTAA GACACTGGGT 6481 GTGAACCAGC GCGGCGAGCT GTGCGTCCGT GGCCCCATGA TCATGAGCGG CTACGTTAAC 6541 AACCCCGAGG CTACAAACGC TCTCATCGAC AAGGACGGCT GGCTGCACAG CGGCGACATC 6601 GCCTACTGGG ACGAGGACGA GCACTTCTTC ATCGTGGACC GGCTGAAGAG CCTGATCAAA 6661 TACAAGGGCT ACCAGGTAGC CCCAGCCGAA CTGGAGAGCA TCCTGCTGCA ACACCCCAAC 6721 ATCTTCGACG CCGGGGTCGC CGGCCTGCCC GACGACGATG CCGGCGAGCT GCCCGCCGCA 6781 GTCGTCGTGC TGGAACACGG TAAAACCATG ACCGAGAAGG AGATCGTGGA CTATGTGGCC 6841 AGCCAGGTTA CAACCGCCAA GAAGCTGCGC GGTGGTGTTG TGTTCGTGGA CGAGGTGCCT 6901 AAAGGACTGA CCGGCAAGTT GGACGCCCGC AAGATCCGCG AGATTCTCAT TAAGGCCAAG 6961 AAGGGCGGCA AGATCGCCGT GTAGtctaga taattctacc gggtagggga ggcgcttttc 7021 ccaaggcagt ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt 7081 ggcctctggc ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt 7141 ggtggcccct tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg 7201 cagctcgcgt cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag 7261 atggacagca ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag 7321 ctttgctcct tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc 7381 aggggcgggc tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc 7441 acgcttcaaa agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg 7501 acctgcagcc caagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg 7561 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc 7621 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc 7681 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg 7741 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc 7801 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg 7861 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc 7921 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg 7981 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc 8041 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga 8101 cccgcaagcc cggtgcctga ccgcgtctgg aacaatcaac ctctggatta caaaatttgt 8161 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 8221 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 8281 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 8341 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 8401 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 8461 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 8521 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc 8581 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 8641 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc 8701 tccctttggg ccgcctcccc gcctggaatt aattctgcag tcgagaccta gaaaaacatg 8761 gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg ctagaagcac 8821 aagaggagga ggaggtgggt tttccagtca cacctcaggt acctttaaga ccaatgactt 8881 acaaggcagc tgtagatctt agccactttt taaaagaaaa gaggggactg gaagggctaa 8941 ttcactccca acgaagacaa gatatccttg atctgtggat ctaccacaca caaggctact 9001 tccctgatta gcagaactac acaccagggc caggggtcag atatccactg acctttggat 9061 ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat aaaggagaga 9121 acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag agagaagtgt 9181 tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg 9241 agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct ggggactttc 9301 cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc tgcatataag 9361 cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc 9421 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa 9481 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag 9541 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa 9601 cttgcaaaga aatgaatatc agagagtgag aggccttgac attgctagcg ttttaccgtc 9661 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta 9721 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc 9781 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg 9841 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg 9901 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 9961 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 10021 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 10081 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 10141 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 10201 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 10261 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 10321 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 10381 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 10441 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 10501 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct 10561 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 10621 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 10681 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 10741 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 10801 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 10861 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 10921 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 10981 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 11041 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 11101 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 11161 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 11221 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 11281 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 11341 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 11401 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 11461 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 11521 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 11581 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 11641 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 11701 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 11761 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 11821 atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg tttattgcag 11881 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 11941 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca 12001 actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca 12061 ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat 12121 tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttttt aaagaaattg 12181 tatttgttaa atatgtacta caaacttagt agt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.