pCMV-GRIN1-FLAG-TwinStrep-Neo

pCMV-GRIN1-FLAG-TwinStrep-Neo plasmid

PVT95379

pCMV-GRIN1-FLAG-TwinStrep-Neo plasmid Information

Plasmid name: pCMV-GRIN1(human)-FLAG-TwinStrep-Neo
Alias: NM_007327.4;DEE101; GluN1; MRD8; NDHMSD; NDHMSR;NMD-R1; NMDA1; NMDAR1; NR1
Gene lenght: 2814bp
Host: Mammalian cells
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Neo/G418
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pCMV-GRIN1-FLAG-TwinStrep-Neo plasmid Description

pCMV-GRIN1-FLAG-TwinStrep-Neo is a plasmid suitable for protein expression in mammalian cells.

pCMV-GRIN1-FLAG-TwinStrep-Neo plasmid Sequence

1 GACGGATCGG GAGATCTCCC GATCCCCTAT GGTGCACTCT CAGTACAATC TGCTCTGATG 61 CCGCATAGTT AAGCCAGTAT CTGCTCCCTG CTTGTGTGTT GGAGGTCGCT GAGTAGTGCG 121 CGAGCAAAAT TTAAGCTACA ACAAGGCAAG GCTTGACCGA CAATTGCATG AAGAATCTGC 181 TTAGGGTTAG GCGTTTTGCG CTGCTTCGCG ATGTACGGGC CAGATATACG CGTTGACATT 241 GATTATTGAC TAGTTATTAA TAGTAATCAA TTACGGGGTC ATTAGTTCAT AGCCCATATA 301 TGGAGTTCCG CGTTACATAA CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC 361 CCCGCCCATT GACGTCAATA ATGACGTATG TTCCCATAGT AACGCCAATA GGGACTTTCC 421 ATTGACGTCA ATGGGTGGAG TATTTACGGT AAACTGCCCA CTTGGCAGTA CATCAAGTGT 481 ATCATATGCC AAGTACGCCC CCTATTGACG TCAATGACGG TAAATGGCCC GCCTGGCATT 541 ATGCCCAGTA CATGACCTTA TGGGACTTTC CTACTTGGCA GTACATCTAC GTATTAGTCA 601 TCGCTATTAC CATGGTGATG CGGTTTTGGC AGTACATCAA TGGGCGTGGA TAGCGGTTTG 661 ACTCACGGGG ATTTCCAAGT CTCCACCCCA TTGACGTCAA TGGGAGTTTG TTTTGGCACC 721 AAAATCAACG GGACTTTCCA AAATGTCGTA ACAACTCCGC CCCATTGACG CAAATGGGCG 781 GTAGGCGTGT ACGGTGGGAG GTCTATATAA GCAGAGCTCT CTGGCTAACT AGAGAACCCA 841 CTGCTTACTG GCTTATCGAA ATTAATACGA CTCACTATAG GGAGACCCAA GCTGGCTAGC 901 GTTTAAACTT AAGCTGAATT Cgccaccatg agcaccatgc gcctgctgac gctcgccctg 961 ctgttctcct gctccgtcgc ccgtgccgcg tgcgacccca agatcgtcaa cattggcgcg 1021 gtgctgagca cgcggaagca cgagcagatg ttccgcgagg ccgtgaacca ggccaacaag 1081 cggcacggct cctggaagat tcagctcaat gccacctccg tcacgcacaa gcccaacgcc 1141 atccagatgg ctctgtcggt gtgcgaggac ctcatctcca gccaggtcta cgccatccta 1201 gttagccatc cacctacccc caacgaccac ttcactccca cccctgtctc ctacacagcc 1261 ggcttctacc gcatacccgt gctggggctg accacccgca tgtccatcta ctcggacaag 1321 agcatccacc tgagcttcct gcgcaccgtg ccgccctact cccaccagtc cagcgtgtgg 1381 tttgagatga tgcgtgtcta cagctggaac cacatcatcc tgctggtcag cgacgaccac 1441 gagggccggg cggctcagaa acgcctggag acgctgctgg aggagcgtga gtccaaggca 1501 gagaaggtgc tgcagtttga cccagggacc aagaacgtga cggccctgct gatggaggcg 1561 aaagagctgg aggcccgggt catcatcctt tctgccagcg aggacgatgc tgccactgta 1621 taccgcgcag ccgcgatgct gaacatgacg ggctccgggt acgtgtggct ggtcggcgag 1681 cgcgagatct cggggaacgc cctgcgctac gccccagacg gcatcctcgg gctgcagctc 1741 atcaacggca agaacgagtc ggcccacatc agcgacgccg tgggcgtggt ggcccaggcc 1801 gtgcacgagc tcctcgagaa ggagaacatc accgacccgc cgcggggctg cgtgggcaac 1861 accaacatct ggaagaccgg gccgctcttc aagagagtgc tgatgtcttc caagtatgcg 1921 gatggggtga ctggtcgcgt ggagttcaat gaggatgggg accggaagtt cgccaactac 1981 agcatcatga acctgcagaa ccgcaagctg gtgcaagtgg gcatctacaa tggcacccac 2041 gtcatcccta atgacaggaa gatcatctgg ccaggcggag agacagagaa gcctcgaggg 2101 taccagatgt ccaccagact gaagattgtg acgatccacc aggagccctt cgtgtacgtc 2161 aagcccacgc tgagtgatgg gacatgcaag gaggagttca cagtcaacgg cgacccagtc 2221 aagaaggtga tctgcaccgg gcccaacgac acgtcgccgg gcagcccccg ccacacggtg 2281 cctcagtgtt gctacggctt ttgcatcgac ctgctcatca agctggcacg gaccatgaac 2341 ttcacctacg aggtgcacct ggtggcagat ggcaagttcg gcacacagga gcgggtgaac 2401 aacagcaaca agaaggagtg gaatgggatg atgggcgagc tgctcagcgg gcaggcagac 2461 atgatcgtgg cgccgctaac cataaacaac gagcgcgcgc agtacatcga gttttccaag 2521 cccttcaagt accagggcct gactattctg gtcaagaagg agattccccg gagcacgctg 2581 gactcgttca tgcagccgtt ccagagcaca ctgtggctgc tggtggggct gtcggtgcac 2641 gtggtggccg tgatgctgta cctgctggac cgcttcagcc ccttcggccg gttcaaggtg 2701 aacagcgagg aggaggagga ggacgcactg accctgtcct cggccatgtg gttctcctgg 2761 ggcgtcctgc tcaactccgg catcggggaa ggcgccccca gaagcttctc agcgcgcatc 2821 ctgggcatgg tgtgggccgg ctttgccatg atcatcgtgg cctcctacac cgccaacctg 2881 gcggccttcc tggtgctgga ccggccggag gagcgcatca cgggcatcaa cgaccctcgg 2941 ctgaggaacc cctcggacaa gtttatctac gccacggtga agcagagctc cgtggatatc 3001 tacttccggc gccaggtgga gctgagcacc atgtaccggc atatggagaa gcacaactac 3061 gagagtgcgg cggaggccat ccaggccgtg agagacaaca agctgcatgc cttcatctgg 3121 gactcggcgg tgctggagtt cgaggcctcg cagaagtgcg acctggtgac gactggagag 3181 ctgtttttcc gctcgggctt cggcataggc atgcgcaaag acagcccctg gaagcagaac 3241 gtctccctgt ccatcctcaa gtcccacgag aatggcttca tggaagacct ggacaagacg 3301 tgggttcggt atcaggaatg tgactcgcgc agcaacgccc ctgcgaccct tacttttgag 3361 aacatggccg gggtcttcat gctggtagct gggggcatcg tggccgggat cttcctgatt 3421 ttcatcgaga ttgcctacaa gcggcacaag gatgctcgcc ggaagcagat gcagctggcc 3481 tttgccgccg ttaacgtgtg gcggaagaac ctgcaggata gaaagagtgg tagagcagag 3541 cctgacccta aaaagaaagc cacatttagg gctatcacct ccaccctggc ttccagcttc 3601 aagaggcgta ggtcctccaa agacacgagc accgggggtg gacgcggcgc tttgcaaaac 3661 caaaaagaca cagtgctgcc gcgacgcgct attgagaggg aggagggcca gctgcagctg 3721 tgttcccgtc atagggagag cCTCGAGGAT TACAAGGATG ACGACGATAA GGGATCCagc 3781 gcctggtccc accctcagtt tgagaagggc ggaggctctg gcggcggaag cggaggatct 3841 gcttggagcc acccccagtt cgaaaagtga TGAGGGCCCG TTTAAACCCG CTGATCAGCC 3901 TCGACTGTGC CTTCTAGTTG CCAGCCATCT GTTGTTTGCC CCTCCCCCGT GCCTTCCTTG 3961 ACCCTGGAAG GTGCCACTCC CACTGTCCTT TCCTAATAAA ATGAGGAAAT TGCATCGCAT 4021 TGTCTGAGTA GGTGTCATTC TATTCTGGGG GGTGGGGTGG GGCAGGACAG CAAGGGGGAG 4081 GATTGGGAAG ACAATAGCAG GCATGCTGGG GATGCGGTGG GCTCTATGGC TTCTGAGGCG 4141 GAAAGAACCA GCTGGGGCTC TAGGGGGTAT CCCCACGCGC CCTGTAGCGG CGCATTAAGC 4201 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 4261 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 4321 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 4381 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 4441 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 4501 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 4561 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTAATTCTG TGGAATGTGT 4621 GTCAGTTAGG GTGTGGAAAG TCCCCAGGCT CCCCAGCAGG CAGAAGTATG CAAAGCATGC 4681 ATCTCAATTA GTCAGCAACC AGGTGTGGAA AGTCCCCAGG CTCCCCAGCA GGCAGAAGTA 4741 TGCAAAGCAT GCATCTCAAT TAGTCAGCAA CCATAGTCCC GCCCCTAACT CCGCCCATCC 4801 CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA TGGCTGACTA ATTTTTTTTA 4861 TTTATGCAGA GGCCGAGGCC GCCTCTGCCT CTGAGCTATT CCAGAAGTAG TGAGGAGGCT 4921 TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TCCCGGGAGC TTGTATATCC ATTTTCGGAT 4981 CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA TTGCACGCAG 5041 GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA CAGACAATCG 5101 GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA 5161 AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG CTATCGTGGC 5221 TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA GCGGGAAGGG 5281 ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC CTTGCTCCTG 5341 CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT GATCCGGCTA 5401 CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT CGGATGGAAG 5461 CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG CCAGCCGAAC 5521 TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG ACCCATGGCG 5581 ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC ATCGACTGTG 5641 GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT GATATTGCTG 5701 AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC GCCGCTCCCG 5761 ATTCGCAGCG CATCGCCTTC TATCGCCTTC TTGACGAGTT CTTCTGAGCG GGACTCTGGG 5821 GTTCGAAATG ACCGACCAAG CGACGCCCAA CCTGCCATCA CGAGATTTCG ATTCCACCGC 5881 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT 5941 CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA 6001 TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT 6061 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC 6121 GACCTCTAGC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA 6181 TCCGCTCACA ATTCCACACA ACATACGAGC CGGAAGCATA AAGTGTAAAG CCTGGGGTGC 6241 CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT TCCAGTCGGG 6301 AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG GCGGTTTGCG 6361 TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG TTCGGCTGCG 6421 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 6481 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 6541 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 6601 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 6661 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 6721 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 6781 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 6841 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 6901 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 6961 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 7021 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 7081 TGGTAGCGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 7141 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 7201 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 7261 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG TCTGACAGTT ACCAATGCTT 7321 AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT TCATCCATAG TTGCCTGACT 7381 CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA TCTGGCCCCA GTGCTGCAAT 7441 GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA GCAATAAACC AGCCAGCCGG 7501 AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC TCCATCCAGT CTATTAATTG 7561 TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT TTGCGCAACG TTGTTGCCAT 7621 TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG GCTTCATTCA GCTCCGGTTC 7681 CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC AAAAAAGCGG TTAGCTCCTT 7741 CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG TTATCACTCA TGGTTATGGC 7801 AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA TGCTTTTCTG TGACTGGTGA 7861 GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA CCGAGTTGCT CTTGCCCGGC 7921 GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA AAAGTGCTCA TCATTGGAAA 7981 ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG TTGAGATCCA GTTCGATGTA 8041 ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT TTCACCAGCG TTTCTGGGTG 8101 AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA AGGGCGACAC GGAAATGTTG 8161 AATACTCATA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 8221 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT 8281 TCCCCGAAAA GTGCCACCTG ACGTC

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.