pB3GNT5-Fluc plasmid

pB3GNT5-Fluc plasmid

PVT93437

pB3GNT5-Fluc plasmid Information

Plasmid name: pB3GNT5(human)-Fluc
Alias: -
Gene lenght: 722bp
Host: Mammalian cells
Use(s):
Promoter detection
Fragment type: Promoter
Species: Human
Prokaryotic resistance: Amp
Screening marker:
Fluorescent labeling: Fluc
Promoter: B3GNT5
Replicon: pUC
Growth Strain(s): DH5a
Growth Temperature: 37°C
Vector backbone:
Forward primer:
Reverse primer: Fluc
Copy number: High
Induction:

pB3GNT5-Fluc plasmid Description

pB3GNT5-Fluc is a plasmid suitable for promoter detection in mammalian cells.

pB3GNT5-Fluc plasmid Sequence

1 acatctgtgt gttggttttt tgtgtgaatc gatagtacta acatacgctc tccatcaaaa 61 caaaacgaaa caaaacaaac tagcaaaata ggctgtcccc agtgcaagtg caggtgccag 121 aacatttctc tatcgatagg taccCGCTCA AAATATTATC TCCGTTAAAT GTTTTGTGGC 181 TTTAGGGAGA GGTCTAACAA CATGCGGGAA ACAAGAAATC AAGCGCATCC AGGATTCATT 241 TATAATCTCT CTCGTTGAGT AGAAGTCCGC ATCTCTCGAT ATTGTCTGGT TACCTGCATG 301 AAGTATTCTA AAGGAGGAAA ATAGCTCAAA GAGGACATTC ATGTGCACTC TGGCTTCCAG 361 TTGGCCATTT GAGTAAGTGA TCGCAATTAA CTGACGAGCG GCAGGGAAAC ACTTCCTGGA 421 ATTCTCATCT ACAGACAAGA ACAAACTGGG GCGGGGCCCA TCACCTTCAC CTACGCGCCG 481 GGAGGGTGGC GGCTGGCGGG CGGGGCCGGG CTCGGGCCGT GACGCCGAGA GTGCGGGGCG 541 CGCGGCTGGG AGCCTCGCGC CCCCGCCCGG GCCCGCCCCC ATCCCGCCCG CATACAGCCC 601 GCATCCCGCC GGGGAAGCGA GCCCAGTCCA GCGCTGCCCG TCCAGTCCTC GCCCAAGATT 661 TAAAGCCCGC AAGTTTTGTT CTTGAGACCA GCGACTTTAG CTCCGATGCG GGAAGGAAAG 721 CCGACCTCCG ATTTGGACAT TTAAAGAGCT GGGCTTGAAC TTCGTGAGTT TCGCTCTAAA 781 CTGCCCTTGA AATGAAGCTG GACTTGGAGG TAAAGTCACT GGGAAGCTGG CCTGGGGCGG 841 GGTTTCCCCC TCTTCTCGTA TTTTAGaagc ttggcattcc ggtactgttg gtaaagccac 901 catggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatccgc tggaagatgg 961 aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat 1021 tgcttttaca gatgcacata tcgaggtgga catcacttac gctgagtact tcgaaatgtc 1081 cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt 1141 atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt 1201 tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgggcat 1261 ttcgcagcct accgtggtgt tcgtttccaa aaaggggttg caaaaaattt tgaacgtgca 1321 aaaaaagctc ccaatcatcc aaaaaattat tatcatggat tctaaaacgg attaccaggg 1381 atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga 1441 ttttgtgcca gagtccttcg atagggacaa gacaattgca ctgatcatga actcctctgg 1501 atctactggt ctgcctaaag gtgtcgctct gcctcataga actgcctgcg tgagattctc 1561 gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt 1621 tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt 1681 tcgagtcgtc ttaatgtata gatttgaaga agagctgttt ctgaggagcc ttcaggatta 1741 caagattcaa agtgcgctgc tggtgccaac cctattctcc ttcttcgcca aaagcactct 1801 gattgacaaa tacgatttat ctaatttaca cgaaattgct tctggtggcg ctcccctctc 1861 taaggaagtc ggggaagcgg ttgccaagag gttccatctg ccaggtatca ggcaaggata 1921 tgggctcact gagactacat cagctattct gattacaccc gagggggatg ataaaccggg 1981 cgcggtcggt aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa 2041 aacgctgggc gttaatcaaa gaggcgaact gtgtgtgaga ggtcctatga ttatgtccgg 2101 ttatgtaaac aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc 2161 tggagacata gcttactggg acgaagacga acacttcttc atcgttgacc gcctgaagtc 2221 tctgattaag tacaaaggct atcaggtggc tcccgctgaa ttggaatcca tcttgctcca 2281 acaccccaac atcttcgacg caggtgtcgc aggtcttccc gacgatgacg ccggtgaact 2341 tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga 2401 ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga 2461 cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat 2521 aaaggccaag aagggcggaa agatcgccgt gtaattctag agtcggggcg gccggccgct 2581 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg 2641 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag 2701 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga 2761 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga 2821 tccgtcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg 2881 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg 2941 tgccggcagc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 3001 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 3061 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 3121 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 3181 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 3241 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 3301 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg 3361 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 3421 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 3481 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 3541 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 3601 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 3661 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 3721 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 3781 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 3841 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 3901 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 3961 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 4021 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 4081 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 4141 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 4201 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 4261 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 4321 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 4381 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 4441 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 4501 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 4561 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 4621 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 4681 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 4741 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 4801 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 4861 acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt 4921 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 4981 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 5041 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 5101 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 5161 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 5221 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 5281 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt 5341 tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 5401 ctattacgcc agcccaagct accatgataa gtaagtaata ttaaggtacg ggaggtactt 5461 ggagcggccg caataaaata tctttatttt catt

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.