pLV3-CMV-ICAM1-Puro plasmid

pLV3-CMV-ICAM1-Puro plasmid

PVT93426

pLV3-CMV-ICAM1-Puro plasmid Information

Plasmid name: pLV3-CMV-ICAM1(human)-Puro
Alias: NM_000201.3;BB2; CD54; P3.58;
Gene lenght: 1599bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Human
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling:
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-ICAM1-Puro plasmid Description

pLV3-CMV-ICAM1-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-ICAM1-Puro plasmid Sequence

1 ACGCGTGTAG TCTTATGCAA TACTCTTGTA GTCTTGCAAC ATGGTAACGA TGAGTTAGCA 61 ACATGCCTTA CAAGGAGAGA AAAAGCACCG TGCATGCCGA TTGGTGGAAG TAAGGTGGTA 121 CGATCGTGCC TTATTAGGAA GGCAACAGAC GGGTCTGACA TGGATTGGAC GAACCACTGA 181 ATTGCCGCAT TGCAGAGATA TTGTATTTAA GTGCCTAGCT CGATACAATA AACGGGTCTC 241 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 301 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 361 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 421 CCCGAACAGG GACCTGAAAG CGAAAGGGAA ACCAGAGCTC TCTCGACGCA GGACTCGGCT 481 TGCTGAAGCG CGCACGGCAA GAGGCGAGGG GCGGCGACTG GTGAGTACGC CAAAAATTTT 541 GACTAGCGGA GGCTAGAAGG AGAGAGATGG GTGCGAGAGC GTCAGTATTA AGCGGGGGAG 601 AATTAGATCG CGATGGGAAA AAATTCGGTT AAGGCCAGGG GGAAAGAAAA AATATAAATT 661 AAAACATATA GTATGGGCAA GCAGGGAGCT AGAACGATTC GCAGTTAATC CTGGCCTGTT 721 AGAAACATCA GAAGGCTGTA GACAAATACT GGGACAGCTA CAACCATCCC TTCAGACAGG 781 ATCAGAAGAA CTTAGATCAT TATATAATAC AGTAGCAACC CTCTATTGTG TGCATCAAAG 841 GATAGAGATA AAAGACACCA AGGAAGCTTT AGACAAGATA GAGGAAGAGC AAAACAAAAG 901 TAAGACCACC GCACAGCAAG CGGCCACTGA TCTTCAGACC TGGAGGAGGA GATATGAGGG 961 ACAATTGGAG AAGTGAATTA TATAAATATA AAGTAGTAAA AATTGAACCA TTAGGAGTAG 1021 CACCCACCAA GGCAAAGAGA AGAGTGGTGC AGAGAGAAAA AAGAGCAGTG GGAATAGGAG 1081 CTTTGTTCCT TGGGTTCTTG GGAGCAGCAG GAAGCACTAT GGGCGCAGCC TCAATGACGC 1141 TGACGGTACA GGCCAGACAA TTATTGTCTG GTATAGTGCA GCAGCAGAAC AATTTGCTGA 1201 GGGCTATTGA GGCGCAACAG CATCTGTTGC AACTCACAGT CTGGGGCATC AAGCAGCTCC 1261 AGGCAAGAAT CCTGGCTGTG GAAAGATACC TAAAGGATCA ACAGCTCCTG GGGATTTGGG 1321 GTTGCTCTGG AAAACTCATT TGCACCACTG CTGTGCCTTG GAATGCTAGT TGGAGTAATA 1381 AATCTCTGGA ACAGATTGGA ATCACACGAC CTGGATGGAG TGGGACAGAG AAATTAACAA 1441 TTACACAAGC TTAATACACT CCTTAATTGA AGAATCGCAA AACCAGCAAG AAAAGAATGA 1501 ACAAGAATTA TTGGAATTAG ATAAATGGGC AAGTTTGTGG AATTGGTTTA ACATAACAAA 1561 TTGGCTGTGG TATATAAAAT TATTCATAAT GATAGTAGGA GGCTTGGTAG GTTTAAGAAT 1621 AGTTTTTGCT GTACTTTCTA TAGTGAATAG AGTTAGGCAG GGATATTCAC CATTATCGTT 1681 TCAGACCCAC CTCCCAACCC CGAGGGGACC CGACAGGCCC GAAGGAATAG AAGAAGAAGG 1741 TGGAGAGAGA GACAGAGACA GATCCATTCG ATTAGTGAAC GGATCTCGAC GGTATCGGTT 1801 AACTTTTAAA AGAAAAGGGG GGATTGGGGG GTACAGTGCA GGGGAAAGAA TAGTAGACAT 1861 AATAGCAACA GACATACAAA CTAAAGAATT ACAAAAACAA ATTACAAAAT TCAAAATTTT 1921 ATCGATACTA GTATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA 1981 TCTACGTATT AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC 2041 GTGGATAGCG GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA 2101 GTTTGTTTTG GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT 2161 TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTTTA TATAAGCAGA GCTCGTTTAG 2221 TGAACCGTCA GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGATTCT 2281 AGAGCTAGCG AATTCGCCAC Catggctccc agcagccccc ggcccgcgct gcccgcactc 2341 ctggtcctgc tcggggctct gttcccagga cctggcaatg cccagacatc tgtgtccccc 2401 tcaaaagtca tcctgccccg gggaggctcc gtgctggtga catgcagcac ctcctgtgac 2461 cagcccaagt tgttgggcat agagaccccg ttgcctaaaa aggagttgct cctgcctggg 2521 aacaaccgga aggtgtatga actgagcaat gtgcaagaag atagccaacc aatgtgctat 2581 tcaaactgcc ctgatgggca gtcaacagct aaaaccttcc tcaccgtgta ctggactcca 2641 gaacgggtgg aactggcacc cctcccctct tggcagccag tgggcaagaa ccttacccta 2701 cgctgccagg tggagggtgg ggcaccccgg gccaacctca ccgtggtgct gctccgtggg 2761 gagaaggagc tgaaacggga gccagctgtg ggggagcccg ctgaggtcac gaccacggtg 2821 ctggtgagga gagatcacca tggagccaat ttctcgtgcc gcactgaact ggacctgcgg 2881 ccccaagggc tggagctgtt tgagaacacc tcggccccct accagctcca gacctttgtc 2941 ctgccagcga ctcccccaca acttgtcagc ccccgggtcc tagaggtgga cacgcagggg 3001 accgtggtct gttccctgga cgggctgttc ccagtctcgg aggcccaggt ccacctggca 3061 ctgggggacc agaggttgaa ccccacagtc acctatggca acgactcctt ctcggccaag 3121 gcctcagtca gtgtgaccgc agaggacgag ggcacccagc ggctgacgtg tgcagtaata 3181 ctggggaacc agagccagga gacactgcag acagtgacca tctacagctt tccggcgccc 3241 aacgtgattc tgacgaagcc agaggtctca gaagggaccg aggtgacagt gaagtgtgag 3301 gcccacccta gagccaaggt gacgctgaat ggggttccag cccagccact gggcccgagg 3361 gcccagctcc tgctgaaggc caccccagag gacaacgggc gcagcttctc ctgctctgca 3421 accctggagg tggccggcca gcttatacac aagaaccaga cccgggagct tcgtgtcctg 3481 tatggccccc gactggacga gagggattgt ccgggaaact ggacgtggcc agaaaattcc 3541 cagcagactc caatgtgcca ggcttggggg aacccattgc ccgagctcaa gtgtctaaag 3601 gatggcactt tcccactgcc catcggggaa tcagtgactg tcactcgaga tcttgagggc 3661 acctacctct gtcgggccag gagcactcaa ggggaggtca cccgcaaggt gaccgtgaat 3721 gtgctctccc cccggtatga gattgtcatc atcactgtgg tagcagccgc agtcataatg 3781 ggcactgcag gcctcagcac gtacctctat aaccgccagc ggaagatcaa gaaatacaga 3841 ctacaacagg cccaaaaagg gacccccatg aaaccgaaca cacaagccac gcctccctga 3901 GGATCCGCGG CCGCGAAGGA TCTGCGATCG CTCCGGTGCC CGTCAGTGGG CAGAGCGCAC 3961 ATCGCCCACA GTCCCCGAGA AGTTGGGGGG AGGGGTCGGC AATTGAACGG GTGCCTAGAG 4021 AAGGTGGCGC GGGGTAAACT GGGAAAGTGA TGTCGTGTAC TGGCTCCGCC TTTTTCCCGA 4081 GGGTGGGGGA GAACCGTATA TAAGTGCAGT AGTCGCCGTG AACGTTCTTT TTCGCAACGG 4141 GTTTGCCGCC AGAACACAGC TGAAGCTTCG AGGGGCTCGC ATCTCTCCTT CACGCGCCCG 4201 CCGCCCTACC TGAGGCCGCC ATCCACGCCG GTTGAGTCGC GTTCTGCCGC CTCCCGCCTG 4261 TGGTGCCTCC TGAACTGCGT CCGCCGTCTA GGTAAGTTTA AAGCTCAGGT CGAGACCGGG 4321 CCTTTGTCCG GCGCTCCCTT GGAGCCTACC TAGACTCAGC CGGCTCTCCA CGCTTTGCCT 4381 GACCCTGCTT GCTCAACTCT ACGTCTTTGT TTCGTTTTCT GTTCTGCGCC GTTACAGATC 4441 CAAGCTGTGA CCGGCGCCTA CGCTAGATGA CCGAGTACAA GCCCACGGTG CGCCTCGCCA 4501 CCCGCGACGA CGTCCCCCGG GCCGTACGCA CCCTCGCCGC CGCGTTCGCC GACTACCCCG 4561 CCACGCGCCA CACCGTCGAC CCGGACCGCC ACATCGAGCG GGTCACCGAG CTGCAAGAAC 4621 TCTTCCTCAC GCGCGTCGGG CTCGACATCG GCAAGGTGTG GGTCGCGGAC GACGGCGCCG 4681 CGGTGGCGGT CTGGACCACG CCGGAGAGCG TCGAAGCGGG GGCGGTGTTC GCCGAGATCG 4741 GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC GGCTGGCCGC GCAGCAACAG ATGGAAGGCC 4801 TCCTGGCGCC GCACCGGCCC AAGGAGCCCG CGTGGTTCCT GGCCACCGTC GGCGTCTCGC 4861 CCGACCACCA GGGCAAGGGT CTGGGCAGCG CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG 4921 AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA CCTCCGCGCC CCGCAACCTC CCCTTCTACG 4981 AGCGGCTCGG CTTCACCGTC ACCGCCGACG TCGAGGTGCC CGAAGGACCG CGCACCTGGT 5041 GCATGACCCG CAAGCCCGGT GCCTGAGTCG ACAATCAACC TCTGGATTAC AAAATTTGTG 5101 AAAGATTGAC TGGTATTCTT AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT 5161 TAATGCCTTT GTATCATGCT ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA 5221 AATCCTGGTT GCTGTCTCTT TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG 5281 TGTGCACTGT GTTTGCTGAC GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC 5341 TCCTTTCCGG GACTTTCGCT TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT 5401 GCCTTGCCCG CTGCTGGACA GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT 5461 CGGGGAAATC ATCGTCCTTT CCTTGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG 5521 GGACGTCCTT CTGCTACGTC CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC 5581 TGCTGCCGGC TCTGCGGCCT CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT 5641 CCCTTTGGGC CGCCTCCCCG CCTGGTACCT TTAAGACCAA TGACTTACAA GGCAGCTGTA 5701 GATCTTAGCC ACTTTTTAAA AGAAAAGGGG GGACTGGAAG GGCTAATTCA CTCCCAACGA 5761 AAATAAGATC TGCTTTTTGC TTGTACTGGG TCTCTCTGGT TAGACCAGAT CTGAGCCTGG 5821 GAGCTCTCTG GCTAACTAGG GAACCCACTG CTTAAGCCTC AATAAAGCTT GCCTTGAGTG 5881 CTTCAAGTAG TGTGTGCCCG TCTGTTGTGT GACTCTGGTA ACTAGAGATC CCTCAGACCC 5941 TTTTAGTCAG TGTGGAAAAT CTCTAGCAGT AGTAGTTCAT GTCATCTTAT TATTCAGTAT 6001 TTATAACTTG CAAAGAAATG AATATCAGAG AGTGAGAGGA ACTTGTTTAT TGCAGCTTAT 6061 AATGGTTACA AATAAAGCAA TAGCATCACA AATTTCACAA ATAAAGCATT TTTTTCACTG 6121 CATTCTAGTT GTGGTTTGTC CAAACTCATC AATGTATCTT ATCATGTCTG GCTCTAGCTA 6181 TCCCGCCCCT AACTCCGCCC AGTTCCGCCC ATTCTCCGCC CCATGGCTGA CTAATTTTTT 6241 TTATTTATGC AGAGGCCGAG GCCGCCTCGG CCTCTGAGCT ATTCCAGAAG TAGTGAGGAG 6301 GCTTTTTTGG AGGCCTAGAC TTTTGCAGAG ACGGCCCAAA TTCGTAATCA TGGTCATAGC 6361 TGTTTCCTGT GTGAAATTGT TATCCGCTCA CAATTCCACA CAACATACGA GCCGGAAGCA 6421 TAAAGTGTAA AGCCTGGGGT GCCTAATGAG TGAGCTAACT CACATTAATT GCGTTGCGCT 6481 CACTGCCCGC TTTCCAGTCG GGAAACCTGT CGTGCCAGCT GCATTAATGA ATCGGCCAAC 6541 GCGCGGGGAG AGGCGGTTTG CGTATTGGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC 6601 TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT 6661 TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG 6721 CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG 6781 AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT 6841 ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA 6901 CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT 6961 GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC 7021 CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA 7081 GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG 7141 TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG 7201 TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT 7261 GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA 7321 CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC 7381 AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA 7441 CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA 7501 CTTGGTCTGA CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT 7561 TTCGTTCATC CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT 7621 TACCATCTGG CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT 7681 TATCAGCAAT AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT 7741 CCGCCTCCAT CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA 7801 ATAGTTTGCG CAACGTTGTT GCCATTGCTA CAGGCATCGT GGTGTCACGC TCGTCGTTTG 7861 GTATGGCTTC ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT 7921 TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG 7981 CAGTGTTATC ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG 8041 TAAGATGCTT TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC 8101 GGCGACCGAG TTGCTCTTGC CCGGCGTCAA TACGGGATAA TACCGCGCCA CATAGCAGAA 8161 CTTTAAAAGT GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC 8221 CGCTGTTGAG ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT 8281 TTACTTTCAC CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG 8341 GAATAAGGGC GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA 8401 GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA 8461 AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA 8521 TTATTATCAT GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTCGCGC 8581 GTTTCGGTGA TGACGGTGAA AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT 8641 GTCTGTAAGC GGATGCCGGG AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG 8701 GGTGTCGGGG CTGGCTTAAC TATGCGGCAT CAGAGCAGAT TGTACTGAGA GTGCACCATA 8761 TGCGGTGTGA AATACCGCAC AGATGCGTAA GGAGAAAATA CCGCATCAGG CGCCATTCGC 8821 CATTCAGGCT GCGCAACTGT TGGGAAGGGC GATCGGTGCG GGCCTCTTCG CTATTACGCC 8881 AGCTGGCGAA AGGGGGATGT GCTGCAAGGC GATTAAGTTG GGTAACGCCA GGGTTTTCCC 8941 AGTCACGACG TTGTAAAACG ACGGCCAGTG CCAAGCTG

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.