pLV3-CMV-Slmap-3×FLAG-CopGFP-P

pLV3-CMV-Slmap-3×FLAG-CopGFP-Puro plasmid

PVT92882

pLV3-CMV-Slmap-3×FLAG-CopGFP-Puro plasmid Information

Plasmid name: pLV3-CMV-Slmap(rat)-3×FLAG-CopGFP-Puro
Alias: NM_001106060.1
Gene lenght: 2361bp
Host: Mammalian cells,lentivirus
Use(s): Protein Expression
Fragment type: ORF
Species: Rat
Prokaryotic resistance: Amp
Screening marker: Puro
Fluorescent labeling: Green
Promoter: CMV
Replicon: pUC
Growth Strain(s): Stbl3
Growth Temperature: 37°C
Vector backbone:
Forward primer: CMV-F
Reverse primer:
Copy number: High
Induction:

pLV3-CMV-Slmap-3×FLAG-CopGFP-Puro plasmid Description

pLV3-CMV-Slmap-3×FLAG-CopGFP-Puro is a plasmid suitable for use in mammalian cells, and can also be used for lentivirus packaging.

pLV3-CMV-Slmap-3×FLAG-CopGFP-Puro plasmid Sequence

1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca 61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta 121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga 181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc 241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct 481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt 541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag 601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt 661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt 721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg 781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag 841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag 901 taagaccacc gcacagcaag cggccactga tcttcagacc tggaggagga gatatgaggg 961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag 1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag 1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc 1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga 1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc 1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg 1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata 1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa 1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga 1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa 1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat 1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt 1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg 1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtATCGGTt 1801 aacttttaaa agaaaagggg ggattggggg gtacagtgca ggggaaagaa tagtagacat 1861 aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt 1921 tatcgatact agtattatgc ccagtacatg accttatggg actttcctac ttggcagtac 1981 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg 2041 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg 2101 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca 2161 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtTt atataagcag agctcgttta 2221 gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca tagaagattc 2281 tagagctagc CTGATACGAA CTCGgaattc GCCACCATGC CGTCAGCCTT GGCCATCTTC 2341 ACTTGCCGCC CGAACTCACA CCCGTTTCAG GAGCGACATG TCTACCTGGA CGAGCCCATC 2401 AAGATCGGCC GCTCAGTGGC ACGCTGTCGA CCAGCGCAGA ATAATGCCAC CTTTGACTGC 2461 AAAGTGCTGT CAAGGAACCA TGCTCTCGTC TGGTTTGATC ACAAGACGAG CAAGTTTTAC 2521 CTCCAAGACA CTAAAAGTAG TAATGGTACC TTTATAAATA GCCAGAGATT GAGTCGAGGC 2581 TCTGAAGAAA GTCCACCATG CGAAATTCTT TCTGGTGACA TTATCCAGTT TGGGGTAGAT 2641 GTGACAGAGA ACACACGGAA AGTTACCCAT GGGTGTATTG TTTCCACAAT AAAACTTTTT 2701 CTACCAGATG GTATGGAAGC CCGACTCCGT TCAGATGTCA TCCATGCTCC ATTACCGAGT 2761 CCTGTAGACA AAGTTGCTGC TAACACTCCA AGTATGTACT CTCAGGAACT GTTCCAACTT 2821 TCTCAGTATC TACAGGAGGC CTTACATCGG GAACAGATGT TGGAACAGAA GTTAGCCACA 2881 CTTCAGCGGC TGCTGGCCAT CACCCAAGAG GCTTCAGATA CTAGTTGGCA GGCTTTAATA 2941 GATGAAGATA GACTCTTATC ACGGTTAGAA GTTATGGGGA ACCAGTTGCA GGCATGCTCC 3001 AAAAATCAAA CAGAAGATAG CTTACGGAAG GAGCTGGTAG CGTTGCAGGA GGATAAACAT 3061 AGCTATGAAA CCACAGCCAA AGAGTCCCTG AGGCGGGTTC TTCAGGAGAA AATTGAAGTG 3121 GTTAGAAAAC TCTCAGAAGT GGAGCGGAGT CTGAGTAACA CTGAAGATGA ATGTACCCAC 3181 CTGAGGGAGA TGAATGAGCG GACTCAGGAA GAGTTGAGAG AATTAGCCAA TAAATATAAT 3241 GGAGCAGTTA ATGAAATTAA AGATTTATCT GATAAATTAA AGGCAGCAGA GGGAAAACAA 3301 GAGGAAATCC AGCAGAAGGG ACAAGCTGAG AAGAAAGAAT TACAGGCTAA AATCGATGAC 3361 ATGGAAGAAA AAGAACAAGA GCTCCAGGCA AAAATAGAAG CTTTGCAAGC TGATAACGAT 3421 TTCACCAACG AGAGGCTGAC AGCTTTACAA GAGAAGCTGA TGGTCCAGGG GCATCTAACC 3481 AAAGTGGTGG AGGAATCCAA GCTTTCCAAA GAAAACCAGG CAAAAGCAAA GGAATCTGAC 3541 TTATCAGATA CTCTGAGTCC AAGCAAAGAA AAAAGCAGCG ACGACACTAC AGACGCCCAG 3601 ATGGATGAGC AAGACCTGAA TGAACCCCTG GCTAAGGTGT CTCTGTTAAA AGATGACTTA 3661 CAGGGTACCC AGGCAGAAAC TGAGGCCAAA CAAGACACTC AACATCTTCG GAAAGAATTG 3721 GTGGAAGCCC AGGAGCTAGC TAGAGCAAGT AAACAAAAAT GCTTTGACCT TCAAGCTCTT 3781 TTGGAAGAAG AACGAAAAGC CTATCGAAAT CAAGTTGAGG AATCAGCTAA ACAAATCCAG 3841 GTTCTTCAAG TCCAGCTGCA GAGGCTACAC ATGGATATGG AGAATCTCCA GGAGGAAAAG 3901 GACACTGAGA TTTCTAGCAC GAGAGATAAA TTGCTGAGTG CTCAAGATGA GATTTTGCTC 3961 CTTCATCAAG CAGCAGCAAA GGCTGTGTCT GAGCGGGACA CTGACTTTAT GTCTTTGCAA 4021 GAAGAGCTCA AGAAGGTGAG AGCAGAGCTT GAGGGCTGGA GGAAAGCAGC ATCTGAGTAT 4081 GAGGAGGAAA TCAGAAGTCT GCAGAGCACT TTCCAACTTA GATGTCAGCA GTGTGAAGTC 4141 CAGCAAAGAG AGGAGGCCAC AAGGTTGCAA GGTGAACTAG AGAAGTTGAA AAAGGAATGG 4201 GATGTACTGG AAAATGAGTG CCGTTCTCTG AAAAAGGAGA ATGTGTTATT GTCATCAGAA 4261 CTGCAGCGAC AAGAAAAAGA ATTGCACAAT TCTCAGAAGC AAAGTTTGGA GCTCACGAGT 4321 GACCTCAGCA TCCTTCAAAT GACTAGGAAA GAACTCGAGA ACCAAATGGG ATCCTTGAAA 4381 GAACAACACC TTCGGGATGA AGCTGACTTA AAAACTCTTC TCAGTAAGGC TGAAAACCAA 4441 GCCAAGGATG TACAGAAAGA GTATGAAAAG ACACAGACTG TACTCTCTGA ACTGAAGTTG 4501 AAGTTTGAAA TGACTGAGCA GGAAAAGCAA TCAATCACAG ATGAGCTCAA ACAATGTAAA 4561 GACAACCTGA AGCTGCTGCG AGAGAAAGGA AATAATCCCT CCATATTACA ACCTGTCCCA 4621 GCCGTTTTCA TCGGCCTATT CCTGGCTTTC CTGTTTTGGT GTTTCGGTCC GTTGTGGgga 4681 tccGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG ACATCGACTA CAAGGATGAC 4741 GATGACAAGt agcggccgcG aaggatctgc gatcgctccg gtgcccgtca gtgggcagag 4801 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aacgggtgcc 4861 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt 4921 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc 4981 aacgggtttg ccgccagaac acagctgaag cttcgagggg ctcgcatctc tccttcacgc 5041 gcccgccgcc ctacctgagg ccgccatcca cgccggttga gtcgcgttct gccgcctccc 5101 gcctgtggtg cctcctgaac tgcgtccgcc gtctaggtaa gtttaaagct caggtcgaga 5161 ccgggccttt gtccggcgct cccttggagc ctacctagac tcagccggct ctccacgctt 5221 tgcctgaccc tgcttgctca actctacgtc tttgtttcgt tttctgttct gcgccgttac 5281 agatccaagc tgtgaccggc gcctacgcta gacgccacca tggagagcga cgagagcggc 5341 ctgcccgcca tggagatcga gtgccgcatc accggcaccc tgaacggcgt ggagttcgag 5401 ctggtgggcg gcggagaggg cacccccaag cagggccgca tgaccaacaa gatgaagagc 5461 accaaaggcg ccctgacctt cagcccctac ctgctgagcc acgtgatggg ctacggcttc 5521 taccacttcg gcacctaccc cagcggctac gagaacccct tcctgcacgc catcaacaac 5581 ggcggctaca ccaacacccg catcgagaag tacgaggacg gcggcgtgct gcacgtgagc 5641 ttcagctacc gctacgaggc cggccgcgtg atcggcgact tcaaggtggt gggcaccggc 5701 ttccccgagg acagcgtgat cttcaccgac aagatcatcc gcagcaacgc caccgtggag 5761 cacctgcacc ccatgggcga taacgtgctg gtgggcagct tcgcccgcac cttcagcctg 5821 cgcgacggcg gctactacag cttcgtggtg gacagccaca tgcacttcaa gagcgccatc 5881 caccccagca tcctgcagaa cgggggcccc atgttcgcct tccgccgcgt ggaggagctg 5941 cacagcaaca ccgagctggg catcgtggag taccagcacg ccttcaagac ccccatcgcc 6001 ttcgccagat cccgcgctca gtcgtccaat tctgccgtgg acggcaccgc cggacccggc 6061 tccaccggat ctcgcgaggg cagaggaagt cttctaacat gcggtgacgt ggaggagaat 6121 cccggcccta tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc 6181 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc 6241 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc 6301 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc 6361 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag 6421 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg 6481 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag 6541 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc 6601 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc 6661 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc 6721 ggtgcctgaa atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac 6781 tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt 6841 gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat 6901 gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca 6961 acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc 7021 cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg 7081 gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaATCATC GTCCTTTCCT 7141 tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct 7201 tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt 7261 ccgcgtctTc gccttcgccc tcagacgagt cggatctccc tttggGccgc ctccccgcct 7321 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga 7381 aaagggggga ctggaagggc taattcactc ccaacgaaGa taagatctgc tttttgcttg 7441 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa 7501 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct 7561 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc 7621 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat 7681 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag 7741 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 7801 actcatcaat gtatcttatc atgtctggct ctagctaTCC CGCCCCTAAC TCCGCCCAtc 7861 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 7921 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc 7981 ttttttggag gcctagactt ttgcagagac caaattcgta atcatgtcat agctgtttcc 8041 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 8101 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 8161 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 8221 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 8281 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 8341 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 8401 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 8461 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 8521 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 8581 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 8641 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 8701 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 8761 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 8821 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 8881 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 8941 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 9001 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 9061 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 9121 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 9181 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 9241 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 9301 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 9361 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 9421 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 9481 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 9541 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 9601 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 9661 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 9721 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 9781 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 9841 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 9901 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 9961 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 10021 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 10081 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 10141 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 10201 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg 10261 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 10321 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 10381 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 10441 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag 10501 gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc 10561 gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg 10621 acgttgtaaa acgacggcca gtgccaagct g

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Caution:
1.This product is FOR RESEARCH USE ONLY!
2.The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20μl ddH2O in to the tube of plasmid.Take 2ul plasmid into 100 μ L corresponding competent cell, centrifugal then full coating on plate.
3.Shipping temperature is 2-8℃.